##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294765_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3331392
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.966495086738515	33.0	33.0	33.0	27.0	33.0
2	30.943528410946534	33.0	33.0	33.0	27.0	33.0
3	30.992719860046492	33.0	33.0	33.0	27.0	33.0
4	34.29505173813229	37.0	37.0	37.0	27.0	37.0
5	34.773836282250784	37.0	37.0	37.0	27.0	37.0
6	34.82898259946593	37.0	37.0	37.0	33.0	37.0
7	34.901275202678036	37.0	37.0	37.0	33.0	37.0
8	34.77380296284556	37.0	37.0	37.0	27.0	37.0
9	34.494620867193056	37.0	37.0	37.0	27.0	37.0
10	34.630564040497184	37.0	37.0	37.0	27.0	37.0
11	34.89072795996388	37.0	37.0	37.0	33.0	37.0
12	34.836871794132904	37.0	37.0	37.0	33.0	37.0
13	34.672978442645	37.0	37.0	37.0	27.0	37.0
14	35.97846635880737	40.0	37.0	40.0	27.0	40.0
15	36.179805618792386	40.0	37.0	40.0	27.0	40.0
16	36.13882064914606	40.0	37.0	40.0	27.0	40.0
17	36.15923463825332	40.0	37.0	40.0	27.0	40.0
18	36.133956015983706	40.0	37.0	40.0	27.0	40.0
19	36.12407906364667	40.0	37.0	40.0	27.0	40.0
20	36.08300494207827	40.0	37.0	40.0	27.0	40.0
21	35.991679754288896	40.0	37.0	40.0	27.0	40.0
22	35.93426081349778	40.0	37.0	40.0	27.0	40.0
23	35.58813883205579	37.0	37.0	40.0	27.0	40.0
24	35.95126031400688	40.0	37.0	40.0	27.0	40.0
25	34.93047290742128	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3188763283454321
1101	2	0.4160015053417574
1101	3	0.2544098167474651
1101	4	0.33762196288786583
1101	5	0.2557078044212915
1101	6	0.14193354666749514
1101	7	0.14801526075603277
1101	8	0.3212715556262893
1101	9	0.5395460535608194
1101	10	0.5321037565992697
1101	11	0.36134255703007057
1101	12	0.32563994492854675
1101	13	0.2674781658706422
1101	14	0.9495514285864175
1101	15	0.7174959545120174
1101	16	0.5651754685785377
1101	17	0.6898392141731762
1101	18	0.6874482760708602
1101	19	0.5988827797927314
1101	20	0.7971117796024885
1101	21	0.7265267107916316
1101	22	0.80747012356764
1101	23	1.0536083998365129
1101	24	0.853185124790734
1101	25	0.6358378310149178
1102	1	0.18310825919514429
1102	2	0.3177708098599439
1102	3	0.26037482476219864
1102	4	0.2395943831100027
1102	5	0.06857533584549458
1102	6	0.19448367190954485
1102	7	0.1924165052186666
1102	8	0.13846628522851034
1102	9	0.372053660926035
1102	10	0.4861169970147472
1102	11	0.21035104165807184
1102	12	0.20861536262311375
1102	13	0.25037552044017275
1102	14	0.8019852170353445
1102	15	0.5949861113582671
1102	16	0.3944714983486364
1102	17	0.5526121982687613
1102	18	0.5992388395274659
1102	19	0.5127514897427474
1102	20	0.6001012941332178
1102	21	0.5992527433280372
1102	22	0.6881544264374071
1102	23	1.0264723691569486
1102	24	0.7015713781017894
1102	25	0.5604148311930217
1103	1	0.12918974440936992
1103	2	0.3344491687927551
1103	3	0.08402626184051343
1103	4	0.06585496584596484
1103	5	0.21137322926142588
1103	6	0.17965894885980305
1103	7	0.1890366580744569
1103	8	0.24698191910577805
1103	9	0.4463609353270499
1103	10	0.3551152826979802
1103	11	0.2627437157122756
1103	12	0.31019768852773666
1103	13	0.2909391617741477
1103	14	0.8260792722590722
1103	15	0.7244894864708655
1103	16	0.33166286780942045
1103	17	0.4447439618741953
1103	18	0.39843116742471807
1103	19	0.45582966818648885
1103	20	0.5475925619865691
1103	21	0.696426096818179
1103	22	0.5393989117224436
1103	23	1.0480014129107218
1103	24	0.7834227167015797
1103	25	0.6061993987191556
1104	1	0.14252003613833963
1104	2	0.3258633435094893
1104	3	0.15667010128259307
1104	4	0.057081989773465125
1104	5	0.08420103566670178
1104	6	0.004044438112046578
1104	7	0.04287779065026598
1104	8	0.2892557753364784
1104	9	0.3770180931821159
1104	10	0.4172255872398267
1104	11	0.20974957783095505
1104	12	0.20983848184021525
1104	13	0.21289251500558493
1104	14	0.6954284658945227
1104	15	0.530236664178787
1104	16	0.4723978370798463
1104	17	0.43744184878340064
1104	18	0.307708771842897
1104	19	0.3516634459625436
1104	20	0.5245980653187559
1104	21	0.5668390439033146
1104	22	0.5737798692750928
1104	23	0.9590151722108899
1104	24	0.7074353750767202
1104	25	0.6564423553525316
1105	1	0.193525870521583
1105	2	0.24692951493914705
1105	3	0.20261476132581535
1105	4	0.2665482548266809
1105	5	0.16782646348700325
1105	6	0.2076688134259328
1105	7	0.2695611600666865
1105	8	0.29828556965292563
1105	9	-1.5286720615467075
1105	10	-0.13789049231911576
1105	11	0.009938941143630586
1105	12	0.17600107938771714
1105	13	0.17927807142332597
1105	14	-1.583565470534566
1105	15	-0.33931215315297436
1105	16	-0.08735785525895778
1105	17	0.2313600945890073
1105	18	0.21616518973234378
1105	19	0.26641705445044295
1105	20	0.4032897535286679
1105	21	0.5574063344794951
1105	22	0.5009978528564716
1105	23	0.7951253895965849
1105	24	0.5211332349830116
1105	25	0.24740502259863462
1106	1	0.010702675047561172
1106	2	0.09460815850865956
1106	3	0.07427738809975892
1106	4	-1.1981644990659746
1106	5	-0.3308969547489724
1106	6	-0.20046175460893778
1106	7	-0.03465517375500582
1106	8	0.11537573551566993
1106	9	0.33158723791318323
1106	10	0.4452987677315363
1106	11	0.18424278974986663
1106	12	0.024951356258277713
1106	13	0.10082988507556223
1106	14	0.5645812489589659
1106	15	0.3554103922065792
1106	16	0.15870162688845113
1106	17	0.0077780320371942935
1106	18	0.16936514170792094
1106	19	0.16000043929646068
1106	20	0.16082889911569254
1106	21	0.4244821011262019
1106	22	0.5031018419666111
1106	23	0.6507855388941479
1106	24	0.3105573555762362
1106	25	0.22313269002298597
1107	1	0.2078592574488347
1107	2	0.276002365192479
1107	3	0.24750999135817153
1107	4	0.297853581124059
1107	5	0.22620129295863478
1107	6	0.12986665714505818
1107	7	0.1736027785452663
1107	8	0.29166804880806296
1107	9	0.4053673973625962
1107	10	0.42948638816763207
1107	11	0.29629437345518284
1107	12	0.25544347666528466
1107	13	0.2572731105394368
1107	14	0.7467462466417132
1107	15	0.5166658077263477
1107	16	0.5496988705788652
1107	17	0.5333661635820164
1107	18	0.5493081579624501
1107	19	0.5078144362455674
1107	20	0.6764293955891532
1107	21	0.5723203963796877
1107	22	0.5516883185999006
1107	23	0.9063847500905027
1107	24	0.8712660123397029
1107	25	0.7612594033348898
1108	1	0.2857734913665837
1108	2	0.2517183008770516
1108	3	0.35978211531897486
1108	4	0.3902768061615589
1108	5	0.4547107780251878
1108	6	0.11708965619928335
1108	7	0.24375044740527585
1108	8	0.2651807607152463
1108	9	0.09842588706889899
1108	10	0.34988711338875333
1108	11	0.23228936297577718
1108	12	0.18944570934660732
1108	13	0.30087770908321687
1108	14	-0.9843896151840639
1108	15	-0.07838370704662623
1108	16	0.22846949651285087
1108	17	0.34549737868766783
1108	18	0.5040219124837719
1108	19	0.5439288136480798
1108	20	0.490588307569503
1108	21	0.6188636999195651
1108	22	0.7157558904527974
1108	23	0.9875419675444093
1108	24	0.7791756096320839
1108	25	0.6930339150533342
1109	1	0.3476343693787669
1109	2	0.21804428890533245
1109	3	0.3820813153974463
1109	4	0.4910757812432749
1109	5	0.4677526308626838
1109	6	0.3184419598132564
1109	7	0.26559642744896905
1109	8	0.43299187787789606
1109	9	0.484805646309475
1109	10	0.4840089463336099
1109	11	0.2669623089017037
1109	12	0.3320618149840868
1109	13	0.29090740607325216
1109	14	0.7636034826211215
1109	15	0.4462095564731783
1109	16	0.4498981355449203
1109	17	0.39977235999530336
1109	18	0.4075493926319993
1109	19	0.4589332791667857
1109	20	0.6392349069350374
1109	21	0.6223809412098689
1109	22	0.6782237004299176
1109	23	1.1138750625446576
1109	24	0.8181296242538636
1109	25	0.6782501657556423
1110	1	0.37810650953366576
1110	2	0.2890156940019253
1110	3	0.4558633606759024
1110	4	0.5941088900858276
1110	5	0.4810296925025739
1110	6	0.3129559131498283
1110	7	0.37335356304878076
1110	8	0.3905744371295512
1110	9	0.4522385545009726
1110	10	0.5069417722212819
1110	11	0.2985857092984645
1110	12	0.36215709585298583
1110	13	0.2652031407484188
1110	14	0.7277622949485334
1110	15	0.607806946022869
1110	16	0.563819331050567
1110	17	0.5501773161046586
1110	18	0.6886012780991351
1110	19	0.5095362965953356
1110	20	0.7178228925630918
1110	21	0.6919572255043107
1110	22	0.7775360732492231
1110	23	0.9278711474367967
1110	24	0.8471063180807903
1110	25	0.7491610846352899
1111	1	0.13869773087612458
1111	2	0.03878222640460649
1111	3	0.20692206898279508
1111	4	0.3219022247066263
1111	5	0.26051799498277717
1111	6	0.10808309608910349
1111	7	0.019375302778968262
1111	8	0.1545380065283979
1111	9	0.1467563766655644
1111	10	0.09250057708789683
1111	11	0.06175507503908051
1111	12	0.06830001802585883
1111	13	-0.04470871402576648
1111	14	-1.7294245067178124
1111	15	-0.6738349829643582
1111	16	-0.18518127589621258
1111	17	0.07528663549661019
1111	18	0.1691810502182065
1111	19	0.12236011649766709
1111	20	0.33576127367049224
1111	21	0.41003354906212763
1111	22	0.15258262882623796
1111	23	-1.703244186245989
1111	24	-0.32644390676540525
1111	25	0.09163839310220112
1112	1	0.02168147103676077
1112	2	-0.0656403232142182
1112	3	0.1591323329853367
1112	4	0.33782600447741373
1112	5	-0.5711321876700239
1112	6	-0.7364484050332294
1112	7	-0.8238422841824331
1112	8	-0.5846446415564941
1112	9	-0.4014070209393594
1112	10	-0.7205390337379782
1112	11	-0.536871710130093
1112	12	-0.6047791625197476
1112	13	-0.5948935232157524
1112	14	-0.3773560252260211
1112	15	-0.5250768939823587
1112	16	-0.5948454656367943
1112	17	-0.7158816753035921
1112	18	-0.8836699305677058
1112	19	-0.6043336083458755
1112	20	-0.6944415009269918
1112	21	-1.6336355316359814
1112	22	-1.5403121401134214
1112	23	-1.2036202064028245
1112	24	-1.3542361765187607
1112	25	-1.6569961078927733
1113	1	0.07253122073189289
1113	2	-0.11330517715471444
1113	3	-0.025113150744864754
1113	4	0.19459275319365332
1113	5	0.20937669998285457
1113	6	0.02101549281363191
1113	7	0.05467979060860273
1113	8	0.15543046941145633
1113	9	0.1928815326990474
1113	10	-0.03737315775426708
1113	11	-0.07023642346236159
1113	12	0.03785338088889745
1113	13	0.017666991456785297
1113	14	0.07905476241349163
1113	15	-0.03239430150333078
1113	16	0.054045079688116004
1113	17	0.04472016733011941
1113	18	0.07411658655594522
1113	19	-0.013185776875509703
1113	20	-0.5695446525338426
1113	21	-0.4302400707100631
1113	22	0.1172505134732802
1113	23	-0.160389672984941
1113	24	0.169958748293233
1113	25	-0.8852748212696113
1114	1	-0.8174999994220968
1114	2	-0.5752777580924402
1114	3	-0.3864347086007669
1114	4	-1.5184130868363752
1114	5	-0.6202424669971407
1114	6	-0.472129938851495
1114	7	-0.4383808962242597
1114	8	-0.3443194380036587
1114	9	-0.37951228265070824
1114	10	-0.4096078645170991
1114	11	-0.4283519114055565
1114	12	-0.49646252352024334
1114	13	-0.4697242069278431
1114	14	-0.5438873401669397
1114	15	-1.271931684131907
1114	16	-0.827064100649217
1114	17	-0.7979743909274148
1114	18	-0.8273031162033675
1114	19	-0.8793598803668985
1114	20	-2.0339445253311297
1114	21	-6.6955191070410045
1114	22	-7.752865178015295
1114	23	-7.876626007058935
1114	24	-5.71869745572867
1114	25	-7.020151152655963
1115	1	-0.8624750653505338
1115	2	-0.946061100249203
1115	3	-0.6214800302433723
1115	4	-0.4368673140860082
1115	5	-0.5158502701220726
1115	6	-0.7984245118503779
1115	7	-1.005294456304938
1115	8	-0.6435443039428819
1115	9	-2.5007486875113543
1115	10	-1.4187414014365203
1115	11	-0.9703908168849296
1115	12	-0.9532229809894304
1115	13	-1.1647701488060207
1115	14	-0.9734804769929752
1115	15	-1.8242418043246076
1115	16	-1.2407570173384102
1115	17	-2.968778946744841
1115	18	-2.6386603714256935
1115	19	-1.9582573875746334
1115	20	-1.5380025235061154
1115	21	-1.00306415248496
1115	22	-0.503923939521151
1115	23	-0.04284262419920992
1115	24	-1.0087451410168597
1115	25	-0.6240805121016564
1116	1	-1.3000606324927482
1116	2	-0.6544087653340469
1116	3	-0.47030796267026176
1116	4	-0.28496028168056853
1116	5	-0.2527701164304972
1116	6	-0.3931326145271896
1116	7	-0.666314977366028
1116	8	-1.6524263520599334
1116	9	-0.7469442190154894
1116	10	-2.273999993901718
1116	11	-0.9777690659902092
1116	12	-0.6916202834840917
1116	13	-0.7160536088985197
1116	14	-1.0876047473907917
1116	15	-1.1563506980388283
1116	16	-0.6159684185133045
1116	17	-0.5351835650184853
1116	18	-1.0669901986543877
1116	19	-0.6798837183577291
1116	20	-0.8702980749555351
1116	21	-0.4216836378966775
1116	22	-0.012370954690851477
1116	23	0.045162358358076915
1116	24	0.03654156300643763
1116	25	0.14887933014244936
1201	1	0.2020766587873979
1201	2	0.31754937176694753
1201	3	0.19087713794341
1201	4	0.27909128579956644
1201	5	0.289048636245397
1201	6	0.15351197897454938
1201	7	0.11495708390603454
1201	8	0.19491982557195797
1201	9	0.41845152809454333
1201	10	0.3687121510961404
1201	11	0.21628031320202723
1201	12	0.2857956339318193
1201	13	0.3070994285402264
1201	14	0.8485606110602788
1201	15	0.6512784347331575
1201	16	0.5993895568446703
1201	17	0.48967234098027745
1201	18	0.4191274931775837
1201	19	0.44261207156662863
1201	20	0.540704446332704
1201	21	0.5692513318954369
1201	22	0.6659836430923178
1201	23	0.5886792224463662
1201	24	0.6206642974148906
1201	25	0.5181289376777727
1202	1	-0.30150340657207764
1202	2	-0.042446480420082366
1202	3	-0.14339159006061308
1202	4	-0.09789250002245353
1202	5	-0.09187356083856457
1202	6	-0.12841687974842841
1202	7	-0.12483929618446155
1202	8	-0.016320621889079234
1202	9	0.04563554016090876
1202	10	0.25125086401720864
1202	11	0.04713036347854427
1202	12	0.030996111558771133
1202	13	0.09989731692635218
1202	14	0.35615525661081193
1202	15	0.3705227786245615
1202	16	0.3358098685591031
1202	17	0.20178508607831702
1202	18	0.15368883053304216
1202	19	0.182016420485688
1202	20	0.3178570355735246
1202	21	0.3276683756360086
1202	22	0.2736681579238862
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2211	5	0.26893168919689714
2211	6	0.14620681224366905
2211	7	0.17115102537543692
2211	8	0.30210599054777276
2211	9	0.3779391071079985
2211	10	0.33769327347960143
2211	11	0.19049123453815753
2211	12	0.1944599319784217
2211	13	0.04132884223542277
2211	14	0.5056224377514908
2211	15	0.19200220511969945
2211	16	0.16468042624239132
2211	17	0.20909063072244294
2211	18	0.21105363789170895
2211	19	0.2597484384459037
2211	20	0.2813971678200815
2211	21	0.3179619749900482
2211	22	0.27200484652253465
2211	23	0.578895622999994
2211	24	0.5219825629000994
2211	25	0.523191392195983
2212	1	-0.09173240270127536
2212	2	-0.16974873627387765
2212	3	-0.15818472414272478
2212	4	-0.3466134689925653
2212	5	-0.12261591441429687
2212	6	-0.42674243974588677
2212	7	-0.3879032596339229
2212	8	-0.1603553798051891
2212	9	-0.044622407678424736
2212	10	-0.35990783894617806
2212	11	-0.3187300178908288
2212	12	-0.49195704219464176
2212	13	-0.4567012933915606
2212	14	-0.2259823314341176
2212	15	-0.4647737186151062
2212	16	-0.2885317774844296
2212	17	-0.250283969699332
2212	18	-0.18970621356874062
2212	19	-0.3557338333375242
2212	20	-0.3085905499331645
2212	21	-0.26767000243366823
2212	22	-0.3497890728648656
2212	23	0.2335140494316832
2212	24	-0.05600373843275719
2212	25	-0.7412261780802538
2213	1	0.019362860115229807
2213	2	-1.0510364564248391
2213	3	-0.01636889719855006
2213	4	0.20367319399245787
2213	5	0.29136795052164643
2213	6	0.13170669985866823
2213	7	0.11290580185475108
2213	8	0.2280233512704939
2213	9	0.2562892346191319
2213	10	-0.14555427080063765
2213	11	0.12122685490722063
2213	12	0.1368553679056035
2213	13	-0.20682530932134569
2213	14	0.23072634603706632
2213	15	0.024449888675476927
2213	16	0.1465400581168339
2213	17	0.3069016011034904
2213	18	0.2062883005501277
2213	19	0.3281771449285813
2213	20	0.3635789833633467
2213	21	-0.3578762935146216
2213	22	-0.6232089567595338
2213	23	0.11481127855940088
2213	24	0.2696789698051205
2213	25	-0.4033030213554767
2214	1	-0.08809205888693583
2214	2	-0.26994801474167573
2214	3	-0.26636877719014507
2214	4	-0.40331790051973115
2214	5	-0.09577132781052455
2214	6	-0.06878619226744576
2214	7	-0.18386609042276092
2214	8	0.09178577610082783
2214	9	0.1657335793765924
2214	10	-1.7096101657879714
2214	11	-0.6227114964864171
2214	12	-0.3015218675429807
2214	13	-0.09208565603811536
2214	14	-0.5406022759902456
2214	15	-0.33548415598448855
2214	16	-0.5366552223461696
2214	17	-0.36326719888060666
2214	18	-0.3639853739802206
2214	19	-1.250088865448916
2214	20	-2.110315075967719
2214	21	-1.4656998143366096
2214	22	-1.7895819548553078
2214	23	-2.1584655279343394
2214	24	-1.2823995101882417
2214	25	-1.558122122123848
2215	1	-0.14504141701435103
2215	2	-0.25510118515911984
2215	3	-0.6802039340458244
2215	4	-0.21797446137842513
2215	5	-0.06464064386224777
2215	6	-0.10619251381157824
2215	7	-0.1443828278828576
2215	8	-0.1590556921076427
2215	9	-0.07053950128424447
2215	10	-0.07619320885003589
2215	11	-0.3955997047392188
2215	12	-0.3382807658588618
2215	13	-0.1889432192881344
2215	14	-0.04995897479604139
2215	15	-0.48504802112390877
2215	16	-0.31643555341921115
2215	17	-0.1391459291849273
2215	18	-0.1539866205997953
2215	19	-2.1118112296219778
2215	20	-2.2124396068929855
2215	21	-3.121739959126465
2215	22	-2.0931190331430116
2215	23	-3.575484341880461
2215	24	-2.390350167711823
2215	25	-1.75276061154851
2216	1	-1.319397103222947
2216	2	-1.0417739553124754
2216	3	-0.6229694707324676
2216	4	-0.7010469824362602
2216	5	-1.6022014632486048
2216	6	-1.227808391711676
2216	7	-0.8906049150705968
2216	8	-4.63294281585166
2216	9	-3.4787601382878535
2216	10	-1.756068564123595
2216	11	-0.7909604622826549
2216	12	-0.9475541869789765
2216	13	-1.6724332339192642
2216	14	-3.300477878905035
2216	15	-1.8502991421614894
2216	16	-2.828364152860253
2216	17	-2.527347614673822
2216	18	-2.3287132027118886
2216	19	-1.3980557321743419
2216	20	-0.5967653359175671
2216	21	-0.5226723704185119
2216	22	-0.4780881062965108
2216	23	-0.8161028572625426
2216	24	-0.2610797471584263
2216	25	-0.16594713259342342
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	118.0
8	524.0
9	1189.0
10	2007.0
11	3236.0
12	4747.0
13	7051.0
14	10303.0
15	15647.0
16	19997.0
17	22134.0
18	21883.0
19	22160.0
20	21920.0
21	23567.0
22	23721.0
23	24429.0
24	25793.0
25	26907.0
26	27872.0
27	30069.0
28	33700.0
29	39007.0
30	47306.0
31	57571.0
32	78409.0
33	113866.0
34	169732.0
35	277385.0
36	501070.0
37	1678066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.580594822976074	19.566420417677442	10.59378104948731	24.25920370985918
2	11.937552650397757	24.32506918479922	35.90560485437418	27.831773310428847
3	21.642471495252888	30.059076589404043	26.93186331892321	21.36658859641986
4	13.106110826890326	20.174411482839872	32.96302567834694	33.75645201192286
5	11.102227693046714	41.14062687642608	34.737150234177975	13.019995196349226
6	31.951078051647773	33.124623623053274	18.551428326121506	16.37286999917745
7	22.975808003323536	34.56285977839287	23.218469402120615	19.24286281616298
8	26.517384874044424	36.29605571036326	22.05270919827951	15.133850217312803
9	26.779250843645897	18.071282299735785	20.459215927121964	34.69025092949636
10	16.252490326113026	32.722097166018635	31.939871363043242	19.08554114482509
11	28.220182483811435	28.85913997990012	19.058019477907546	23.86265805838089
12	22.75238451456885	32.75350720412477	28.400203404931307	16.09390487637507
13	28.245940707356006	23.355247655049503	22.452929135621545	25.94588250197295
14	20.881925718826107	27.804154988534748	25.7908385411523	25.523080751486848
15	31.585309624641734	24.95290160908996	21.254942479326537	22.20684628694177
16	18.456288504008484	29.824642505393527	31.729269185176157	19.989799805421832
17	25.59961360569049	30.656840307962746	27.265984092753847	16.477561993592914
18	25.58045291942746	26.382897355133295	28.798875881369586	19.237773844069665
19	22.550705008769647	32.15649264553366	29.207051618594953	16.085750727101736
20	23.341708241029245	28.33048166225408	27.199779070535335	21.128031026181343
21	18.49739218837169	30.894569064423354	30.55142725901438	20.05661148819057
22	21.141011071066256	30.336277666775718	22.279811113244033	26.242900148913996
23	16.95581615560058	31.483617766305926	27.60504913588614	23.955516942207353
24	20.180041070424583	36.265827287487866	24.355854426627516	19.19827721546003
25	21.87370909689359	27.312252857399454	21.699477606394485	29.114560439312477
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13004.0
1	13004.0
2	14898.0
3	16792.0
4	16792.0
5	16792.0
6	19885.5
7	22979.0
8	22979.0
9	22979.0
10	23648.0
11	24317.0
12	24317.0
13	24317.0
14	28785.5
15	33254.0
16	33254.0
17	33254.0
18	43428.5
19	53603.0
20	53603.0
21	53603.0
22	69559.0
23	85515.0
24	85515.0
25	85515.0
26	106745.5
27	127976.0
28	127976.0
29	127976.0
30	150056.0
31	172136.0
32	172136.0
33	172136.0
34	191756.5
35	211377.0
36	211377.0
37	211377.0
38	230057.5
39	248738.0
40	248738.0
41	248738.0
42	289405.5
43	330073.0
44	330073.0
45	330073.0
46	601422.0
47	872771.0
48	872771.0
49	872771.0
50	738348.5
51	603926.0
52	603926.0
53	603926.0
54	425112.5
55	246299.0
56	246299.0
57	246299.0
58	183115.5
59	119932.0
60	119932.0
61	119932.0
62	97986.0
63	76040.0
64	76040.0
65	76040.0
66	58086.0
67	40132.0
68	40132.0
69	40132.0
70	29382.5
71	18633.0
72	18633.0
73	18633.0
74	13549.5
75	8466.0
76	8466.0
77	8466.0
78	5880.5
79	3295.0
80	3295.0
81	3295.0
82	2329.5
83	1364.0
84	1364.0
85	1364.0
86	945.5
87	527.0
88	527.0
89	527.0
90	351.5
91	176.0
92	176.0
93	176.0
94	114.5
95	53.0
96	53.0
97	53.0
98	33.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030707884271799896
2	0.013748006839183141
3	0.018941031256603846
4	0.01945132845369143
5	0.017770349451520565
6	0.008705069832670548
7	3.0017482181622576E-5
8	5.70332161450829E-4
9	5.403146792692064E-4
10	3.0017482181622576E-5
11	8.404895010854322E-4
12	0.003181853111251993
13	0.002551485985437919
14	6.904020901773193E-4
15	0.0020411887883503353
16	0.0024914510210746737
17	0.0024013985745298064
18	0.002131241234895203
19	4.5026223272433865E-4
20	3.602097861794709E-4
21	0.08221788369546425
22	0.07879589072675926
23	0.07996657253184254
24	0.0012307167694465258
25	0.22972379113595756
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3331392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.111527356179636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.31538659965136	43.37303524960358
2	8.444193043396671	8.294144353032747
3	1.6095964269224592	2.37149216859634
4	0.5364643343427944	1.0538633132676338
5	0.2591435508255586	0.6363467792773475
6	0.14671868131593005	0.43233471186659406
7	0.10021175968583232	0.34450868040551574
8	0.07726765910782533	0.30357862032175414
9	0.0554522073406505	0.2451008337993812
>10	0.37816843754807694	3.5148906360096723
>50	0.0390737420279257	1.32879807302444
>100	0.028081711669520622	2.704114832430449
>500	0.003910523081334702	1.352562634364388
>1k	0.003910523081334196	4.188557460426174
>5k	6.207179494181264E-4	2.3480918991760293
>10k+	0.0018000820533125665	27.508579754398042
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	145736	4.374627783220948	No Hit
CCCATGTACTCTGCGTTGATACCAC	140301	4.211482767563829	No Hit
GAGTACATGGGAAGCAGTGGTATCA	70174	2.1064467946131824	No Hit
CATGTACTCTGCGTTGATACCACTG	57667	1.731018144967629	No Hit
GCGTTGATACCACTGCTTCCCATGT	46101	1.3838359460549823	No Hit
GCTTCCCATGTACTCTGCGTTGATA	39315	1.1801373119704917	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	37211	1.1169805294603576	No Hit
GTATCAACGCAGAGTACATGGGAAG	35869	1.0766970683726202	No Hit
ACTCTGCGTTGATACCACTGCTTCC	30653	0.9201258813132768	No Hit
TATCAACGCAGAGTACATGGGAAGC	28279	0.8488643786141049	No Hit
GGTATCAACGCAGAGTACATGGGAA	24300	0.7294248170134287	No Hit
CAGTGGTATCAACGCAGAGTACATG	20499	0.6153283672410812	No Hit
GTGGTATCAACGCAGAGTACATGGG	17901	0.5373429485332257	No Hit
GCAGTGGTATCAACGCAGAGTACAT	16661	0.5001212706280137	No Hit
ATACCACTGCTTCCCATGTACTCTG	15731	0.47220501219910477	No Hit
GTACTCTGCGTTGATACCACTGCTT	14884	0.4467802047912704	No Hit
CATGGGAAGCAGTGGTATCAACGCA	14190	0.42594807215722436	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	14026	0.4210252050794383	No Hit
GTACATGGTAAGCAGTGGTATCAAC	13877	0.4165526002343765	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	13341	0.4004632297850268	No Hit
ACATGGGAAGCAGTGGTATCAACGC	13153	0.3948199431348817	No Hit
GTTGATACCACTGCTTCCCATGTAC	12178	0.3655528980077997	No Hit
TACCACTGCTTCCCATGTACTCTGC	12131	0.36414207634526347	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	12024	0.3609302057518299	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	11918	0.35774835264057786	No Hit
CCACTGCTTCCCATGTACTCTGCGT	11695	0.35105445411407604	No Hit
CTTCCCATGTACTCTGCGTTGATAC	11212	0.33655601022035236	No Hit
GATACCACTGCTTCCCATGTACTCT	11148	0.3346348913607285	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	10207	0.30638844062782167	No Hit
CCATGTACTCTGCGTTGATACCACT	9726	0.2919500316984612	No Hit
ACCATGTACTCTGCGTTGATACCAC	9672	0.2903290876606536	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	9238	0.27730150039382934	No Hit
GTACATGGGTAAGCAGTGGTATCAA	8732	0.26211265440992837	No Hit
TTCCCATGTACTCTGCGTTGATACC	8214	0.24656359863984784	No Hit
TGATACCACTGCTTCCCATGTACTC	7770	0.23323583655120741	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6677	0.20042672852669396	No Hit
CCCCATGTACTCTGCGTTGATACCA	6370	0.19121136149693582	No Hit
GCTTACCATGTACTCTGCGTTGATA	5365	0.16104379190440513	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5262	0.157951991239698	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4918	0.14762597736921984	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4897	0.14699561024340577	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4210	0.12637359998463105	No Hit
AAAAAGTACTCTGCGTTGATACCAC	3885	0.11661791827560371	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3807	0.11427655466543715	No Hit
TCCATGTACTCTGCGTTGATACCAC	3630	0.10896346031928997	No Hit
ACTCTGCGTTGATACCACTGCTTAC	3608	0.10830307571129427	No Hit
CTGCGTTGATACCACTGCTTCCCAT	3472	0.10422069813459359	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.003496436324515E-5	0.0
4	0.0	0.0	0.0	6.003496436324515E-5	0.0
5	0.0	0.0	0.0	6.003496436324515E-5	0.0
6	0.0	0.0	0.0	6.003496436324515E-5	0.0
7	0.0	0.0	0.0	9.005244654486773E-5	0.0
8	0.0	0.0	0.0	9.005244654486773E-5	0.0
9	0.0	0.0	0.0	9.005244654486773E-5	0.0
10	0.0	0.0	0.0	1.8010489308973546E-4	0.0
11	0.0	0.0	0.0	2.401398574529806E-4	0.0
12	0.0	0.0	0.0	2.701573396346032E-4	0.0
13	0.0	0.0	0.0	2.701573396346032E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCACG	35	0.0021769237	16.278961	11
CGCACGA	50	0.0015048712	13.294484	12
CGCAAAA	395	0.0	12.264234	2
GTACAAA	690	0.0	11.840645	1
TCACGCA	65	8.055628E-4	11.687459	9
AAGCGGT	195	0.0	11.687458	10
GTATCGA	180	0.0	11.619201	18
GGTATCG	185	0.0	11.305168	17
GAACAAA	2210	0.0	10.961599	1
TATCGAC	195	1.8189894E-12	10.740102	19
ACGCAAA	510	0.0	10.617705	1
CTAGCAC	190	9.094947E-12	10.498167	3
ACGCTGC	165	9.367795E-10	10.359338	8
GTCTTAG	215	1.8189894E-12	10.162847	1
GTACAAG	300	0.0	10.1333885	1
GCGTTGT	75	0.0026580617	10.12913	13
GGCCTAC	85	6.596825E-4	10.058879	1
CTATGCG	85	6.6223653E-4	10.054652	9
CGGTGGT	220	1.8189894E-12	9.927699	13
GACTATC	115	1.0323443E-5	9.908933	7
>>END_MODULE