FastQCFastQC Report
Wed 25 May 2016
SRR1294765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331392
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1503624.513488655793134No Hit
GTACATGGGAAGCAGTGGTATCAAC1439634.321406787312931No Hit
GAGTACATGGGAAGCAGTGGTATCA779332.3393524388603923No Hit
CATGTACTCTGCGTTGATACCACTG658491.976621184177665No Hit
GCGTTGATACCACTGCTTCCCATGT501351.5049264691756479No Hit
ACGCAGAGTACATGGGAAGCAGTGG459461.379183236316831No Hit
GCTTCCCATGTACTCTGCGTTGATA433071.2999671008395288No Hit
GTATCAACGCAGAGTACATGGGAAG387081.1619167002862467No Hit
ACTCTGCGTTGATACCACTGCTTCC346981.04154659673794No Hit
TATCAACGCAGAGTACATGGGAAGC336261.0093678558392407No Hit
GGTATCAACGCAGAGTACATGGGAA275490.8269516166215204No Hit
CAGTGGTATCAACGCAGAGTACATG247970.744343505657695No Hit
GTGGTATCAACGCAGAGTACATGGG200320.6013102030622635No Hit
GCAGTGGTATCAACGCAGAGTACAT190640.5722532803104527No Hit
CATGGGAAGCAGTGGTATCAACGCA181600.545117476418266No Hit
ATACCACTGCTTCCCATGTACTCTG171880.5159404837377288No Hit
ACATGGGAAGCAGTGGTATCAACGC168830.506785151672334No Hit
GGGAAGCAGTGGTATCAACGCAGAG158130.47466644573799777No Hit
CTGCTTCCCATGTACTCTGCGTTGA154720.46443048431406453No Hit
AAGCAGTGGTATCAACGCAGAGTAC148160.4447390160029201No Hit
GTACTCTGCGTTGATACCACTGCTT146660.4402363936756767No Hit
GCAGAGTACATGGGAAGCAGTGGTA140000.4202447505427161No Hit
GTACATGGTAAGCAGTGGTATCAAC134450.4035850479319155No Hit
TACCACTGCTTCCCATGTACTCTGC132380.39737142912031964No Hit
CCACTGCTTCCCATGTACTCTGCGT128570.38593476840912144No Hit
CTTCCCATGTACTCTGCGTTGATAC128370.385334418765489No Hit
GATACCACTGCTTCCCATGTACTCT127810.38365343976331817No Hit
GTTGATACCACTGCTTCCCATGTAC126280.3790607649895299No Hit
ATGGGAAGCAGTGGTATCAACGCAG115000.3452010450886596No Hit
CCATGTACTCTGCGTTGATACCACT112560.33787677943634375No Hit
ACCATGTACTCTGCGTTGATACCAC106790.32055669221754746No Hit
GTACATGGGTAAGCAGTGGTATCAA94560.2838453115094231No Hit
GTATCAACGCAGAGTACTTTTTTTT88260.2649342977350008No Hit
TTCCCATGTACTCTGCGTTGATACC85550.2567995600637812No Hit
TGATACCACTGCTTCCCATGTACTC81580.24488261963767696No Hit
GGTAAGCAGTGGTATCAACGCAGAG77930.23392623864138476No Hit
CCCCATGTACTCTGCGTTGATACCA71830.215615574510595No Hit
TATCAACGCAGAGTACTTTTTTTTT71090.2133942808291549No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA69650.20907176339500125No Hit
GGTATCAACGCAGAGTACTTTTTTT66370.19922602923942903No Hit
ATCAACGCAGAGTACATGGGAAGCA63550.19076109926421148No Hit
GTACTTTTTTTTTTTTTTTTTTTTT60900.1828064664860815No Hit
GCTTACCATGTACTCTGCGTTGATA59570.1788141413559257No Hit
GAGTACATGGTAAGCAGTGGTATCA54980.16503611703456092No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54790.1644657848731101No Hit
GGAAGCAGTGGTATCAACGCAGAGT50450.1514381976062859No Hit
GAGTACTTTTTTTTTTTTTTTTTTT44380.133217585922041No Hit
AAAAAGTACTCTGCGTTGATACCAC43230.1297655754711544No Hit
CTGCGTTGATACCACTGCTTCCCAT39760.11934950915413137No Hit
ACTCTGCGTTGATACCACTGCTTAC39180.11760849518759725No Hit
TCCATGTACTCTGCGTTGATACCAC37350.11211529594836032No Hit
ATGGTAAGCAGTGGTATCAACGCAG36410.10929365262328782No Hit
GCGTTGATACCACTGCTTACCATGT36060.10824304074693102No Hit
TGGGAAGCAGTGGTATCAACGCAGA35580.10680220160221314No Hit
CTCTGCGTTGATACCACTGCTTCCC35100.10536136245749524No Hit
ACGCAGAGTACATGGTAAGCAGTGG33920.10181929956006378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGA751.4526075E-512.6895241
TAGACTG2550.011.1910835
CGACCAT600.00589782111.0783346
ATCCGAA600.00590111611.07750212
GTACTTG4000.010.9447151
GTACGGA700.001469901910.8767361
CACGGGA909.396351E-510.5693564
CTATACT2800.010.5316094
CTAGTAC1659.094947E-1010.3771863
TCTACAC2300.010.3395873
GTCTAGT1409.447831E-810.1969391
GAGTTAG1409.447831E-810.1969391
TATCGGT856.629153E-410.05353213
AAAAAGT10550.010.0132961
GGACCAT1252.5871705E-69.8755436
AAAAGTA10700.09.8680252
TTAGACT1356.3350853E-79.8647324
ACTGGTC1951.6552804E-109.7386098
AGTACTC13750.09.6853735
CCCCTAG1002.707488E-49.5171431
Produced by FastQC (version 0.11.5)