FastQCFastQC Report
Wed 25 May 2016
SRR1294764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374944
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38550.16231961679938559No Hit
GTATCAACGCAGAGTACTTTTTTTT33030.1390769634989288No Hit
CCCATGTACTCTGCGTTGATACCAC33010.13899275098697064No Hit
GGTATCAACGCAGAGTACTTTTTTT25280.10644461511513535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAC400.00527898714.2504893
ACTATCG1600.012.46392711
CTATCGG1701.8189894E-1211.73075512
ATCGGTC1855.456968E-1210.77870514
GTACTGG4900.010.6654291
GTATTAG3200.010.3927341
TATCGGT1951.4551915E-1110.22573613
GCACCGT750.002656035110.1298416
TTAGACT1951.6370905E-109.743724
CGGTCAG2054.1836756E-119.73552916
TCGGTCA2054.1836756E-119.73245215
CTAGTAC1804.1818566E-99.5003273
CGTATCC800.0045156529.5003263
TAGAACC1304.2395295E-69.5001274
AGCACCG900.00111469229.4993265
ACACCGT800.0045297959.4967256
GACCGTC800.00453136869.496325511
TATACTG5200.09.3166475
ATTAGAC1554.259873E-79.1938643
CTAGGGC1554.259873E-79.1938643
Produced by FastQC (version 0.11.5)