##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294764_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2374944
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36849837301427	33.0	33.0	33.0	27.0	33.0
2	31.353786868237734	33.0	33.0	33.0	27.0	33.0
3	31.320640402468438	33.0	33.0	33.0	27.0	33.0
4	34.78004028726572	37.0	37.0	37.0	27.0	37.0
5	35.09025349650349	37.0	37.0	37.0	33.0	37.0
6	35.3102831056227	37.0	37.0	37.0	33.0	37.0
7	35.24431733969306	37.0	37.0	37.0	33.0	37.0
8	35.171971633857474	37.0	37.0	37.0	33.0	37.0
9	34.86544272201787	37.0	37.0	37.0	33.0	37.0
10	34.95471935338265	37.0	37.0	37.0	33.0	37.0
11	35.197882560599325	37.0	37.0	37.0	33.0	37.0
12	35.127434583720714	37.0	37.0	37.0	33.0	37.0
13	35.06715653084873	37.0	37.0	37.0	33.0	37.0
14	36.34421780050393	40.0	37.0	40.0	27.0	40.0
15	36.668672187638954	40.0	37.0	40.0	33.0	40.0
16	36.69846741649487	40.0	37.0	40.0	33.0	40.0
17	36.637476083646604	40.0	37.0	40.0	33.0	40.0
18	36.641255120120725	40.0	37.0	40.0	33.0	40.0
19	36.73074101957773	40.0	37.0	40.0	33.0	40.0
20	36.625271164288506	40.0	37.0	40.0	33.0	40.0
21	36.58181245536737	40.0	37.0	40.0	33.0	40.0
22	36.44299612959295	40.0	37.0	40.0	27.0	40.0
23	36.068880360968514	40.0	37.0	40.0	27.0	40.0
24	36.38059634248218	40.0	37.0	40.0	27.0	40.0
25	35.40880248123745	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.408285087262378
1101	2	0.5929077358415
1101	3	0.4533780887712169
1101	4	0.5365762296076113
1101	5	0.4581948557626063
1101	6	0.3709945517800364
1101	7	0.37298611846895113
1101	8	0.4261066112170724
1101	9	0.6614552151542625
1101	10	0.6703439322938891
1101	11	0.4052637274568127
1101	12	0.5547346911100277
1101	13	0.4614792998772401
1101	14	1.0981288045578879
1101	15	0.8602638469620771
1101	16	0.8090796468746575
1101	17	0.7190211336613288
1101	18	0.8273764049631538
1101	19	0.7483125939318356
1101	20	1.0431438613761372
1101	21	0.9679045490401137
1101	22	1.0621400260467908
1101	23	1.3824881033942162
1101	24	1.0072835346260334
1101	25	0.8039553768510359
1102	1	0.3350557955456921
1102	2	0.5352846866218108
1102	3	0.3207250275467288
1102	4	0.3321944769065297
1102	5	0.4134769686077462
1102	6	0.37789731288447825
1102	7	0.30215778713641583
1102	8	0.4279073315051818
1102	9	0.5672175200762339
1102	10	0.65533792989293
1102	11	0.4163681692335217
1102	12	0.4743025182408829
1102	13	0.5401107524582756
1102	14	1.120637808159323
1102	15	0.881872490419461
1102	16	0.6236054571987921
1102	17	0.8129587086913403
1102	18	0.6470042561036067
1102	19	0.6345670957325567
1102	20	0.8738761542933062
1102	21	0.8196452453186254
1102	22	0.9540968087598785
1102	23	1.2186225571757348
1102	24	0.910044639067813
1102	25	0.9564163612447913
1103	1	0.3281050821028799
1103	2	0.49530202689125247
1103	3	0.2958500819660337
1103	4	0.11508212624946879
1103	5	0.3134758423876889
1103	6	0.34872011869985897
1103	7	0.3603036488232192
1103	8	0.4266224727947687
1103	9	0.6551177637269063
1103	10	0.6255821401539876
1103	11	0.4799943233011277
1103	12	0.3754548877198971
1103	13	0.4889315296697774
1103	14	1.1144495808895272
1103	15	0.8293500742410274
1103	16	0.6007588515380533
1103	17	0.6498922507977127
1103	18	0.7927483057023395
1103	19	0.7559033171164486
1103	20	0.899065209686789
1103	21	0.9482853557163438
1103	22	0.8185718863898757
1103	23	1.234571592902597
1103	24	0.9063652429470039
1103	25	0.9232953731837839
1104	1	0.2473586117141977
1104	2	0.4510057735488999
1104	3	0.3276055124168131
1104	4	0.1552922866305977
1104	5	0.1833124760203404
1104	6	0.12370424684971226
1104	7	0.10481662973985095
1104	8	0.27377066959193996
1104	9	0.5116844341251223
1104	10	0.5471130220695457
1104	11	0.42478510515837087
1104	12	0.416517270189793
1104	13	0.35023013219045396
1104	14	0.8355225443095122
1104	15	0.6231549993638907
1104	16	0.5771095610728736
1104	17	0.6391101028529462
1104	18	0.6669203408326609
1104	19	0.6458589711374003
1104	20	0.7509993143720664
1104	21	0.8114850952046524
1104	22	0.8642955649802531
1104	23	1.2119908525947949
1104	24	0.908942139500283
1104	25	0.9331289076724687
1105	1	0.2234207415240803
1105	2	0.2672702808595062
1105	3	0.25718601194048674
1105	4	0.21573589347315902
1105	5	0.32100157845168553
1105	6	0.22069683269240414
1105	7	0.27898371750856654
1105	8	0.3304379437500842
1105	9	-1.535975757234688
1105	10	-0.032522814404792655
1105	11	0.1656478811182751
1105	12	0.21610563948937767
1105	13	0.3059546900333032
1105	14	-1.4312325399799306
1105	15	-0.2245284699646959
1105	16	0.05892118348930353
1105	17	0.2928794769986638
1105	18	0.23043042657179313
1105	19	0.3661565315068671
1105	20	0.3765580270424067
1105	21	0.6099669740100992
1105	22	0.5406274210047712
1105	23	0.9295685355670855
1105	24	0.7081622861266368
1105	25	0.4557729038618419
1106	1	0.0672848055134736
1106	2	0.11003947974881001
1106	3	0.16553753914840996
1106	4	-0.928276291035111
1106	5	0.02248593337728977
1106	6	-0.044290361140745915
1106	7	0.10822869243499866
1106	8	0.2462009674974439
1106	9	0.35962621549521856
1106	10	0.3559576033497791
1106	11	0.25168750149209984
1106	12	0.15923689233864735
1106	13	0.11840551683922484
1106	14	0.5884949248963594
1106	15	0.37763763720924715
1106	16	0.1563419634636105
1106	17	0.01933055246801274
1106	18	0.1884447661631583
1106	19	0.21210820791285556
1106	20	0.35495779495653323
1106	21	0.4470740236184554
1106	22	0.5941298454732546
1106	23	0.8199645735297025
1106	24	0.46527961382359706
1106	25	0.42886826628879504
1107	1	0.2928268279749453
1107	2	0.3187604921407612
1107	3	0.17143179600716607
1107	4	0.3818010223741055
1107	5	0.3105317199519746
1107	6	0.1271329637255505
1107	7	0.1508565610829038
1107	8	0.23994870164889903
1107	9	0.2716090460217515
1107	10	0.36100771476753124
1107	11	0.1296040741789426
1107	12	0.1099404912048314
1107	13	0.10551778365609721
1107	14	0.5906775201092316
1107	15	0.40835309623030724
1107	16	0.41660406776920667
1107	17	0.3203456526140869
1107	18	0.29379309375536167
1107	19	0.29365759760494825
1107	20	0.4493178642201556
1107	21	0.4506291178364279
1107	22	0.44131747721721837
1107	23	0.6742353314577656
1107	24	0.5009252246806213
1107	25	0.5928300854861845
1108	1	0.47705953802806533
1108	2	0.4461532571144353
1108	3	0.5258776354225816
1108	4	0.7628880320762974
1108	5	0.5375934262357376
1108	6	0.48563797276510456
1108	7	0.5002500180731886
1108	8	0.5226914823068185
1108	9	0.3688103824512865
1108	10	0.6705047979395502
1108	11	0.38871617719685503
1108	12	0.43643312034636494
1108	13	0.5114869044350385
1108	14	-0.8501122677237589
1108	15	0.19977859167967438
1108	16	0.4541463362466729
1108	17	0.5221808924213533
1108	18	0.7131845452197965
1108	19	0.5994358261158865
1108	20	0.7170258126406708
1108	21	0.8598900925807555
1108	22	0.9101811374561635
1108	23	1.2526071309727627
1108	24	1.0052683268103735
1108	25	0.9361981494338067
1109	1	0.35610308996546536
1109	2	0.26049613666367577
1109	3	0.2964696225734933
1109	4	0.41197417995240926
1109	5	0.3487154816836622
1109	6	0.14326787878152203
1109	7	0.13804893074244973
1109	8	0.1794427247789585
1109	9	0.325791311038401
1109	10	0.4286445216059178
1109	11	0.24096821005306168
1109	12	0.22559387815834242
1109	13	0.2356976619208524
1109	14	0.6135951517393252
1109	15	0.3323018991307336
1109	16	0.4719382039521136
1109	17	0.4180540801732633
1109	18	0.3910592274897553
1109	19	0.30177863898439483
1109	20	0.5572059520758685
1109	21	0.46606871430461183
1109	22	0.6427042999491235
1109	23	0.938359126502256
1109	24	0.6349329879816779
1109	25	0.4818998879888241
1110	1	0.34989377493634066
1110	2	0.28702350369560037
1110	3	0.3920889322246204
1110	4	0.587735367295835
1110	5	0.47953985785184017
1110	6	0.3259207211428077
1110	7	0.3063417811760232
1110	8	0.3915729082331154
1110	9	0.5147003806237649
1110	10	0.46388234213296187
1110	11	0.16129376378073346
1110	12	0.09628611831074352
1110	13	0.22565845220115222
1110	14	0.6557645660448017
1110	15	0.4275509828108923
1110	16	0.3608296286353223
1110	17	0.399936041060748
1110	18	0.47979427818979303
1110	19	0.47128465568471967
1110	20	0.5471847042326203
1110	21	0.5815439274434127
1110	22	0.676707399957678
1110	23	0.8606008954723734
1110	24	0.6568063271684679
1110	25	0.6181622750799391
1111	1	0.11882728409484855
1111	2	0.036122724226984815
1111	3	0.18349735313077886
1111	4	0.45122034477102346
1111	5	0.22329434197189357
1111	6	0.06950441061948709
1111	7	0.07133808840109879
1111	8	0.10935037904031475
1111	9	0.13902295774860107
1111	10	0.04177068341068235
1111	11	0.01165331490319943
1111	12	-0.07259935738986201
1111	13	0.08319807219726982
1111	14	-1.9122468954522631
1111	15	-0.7343534139746808
1111	16	-0.23828856795174858
1111	17	-0.023700395968262455
1111	18	0.0011461441618365598
1111	19	0.032313009826800965
1111	20	0.12244134752557301
1111	21	0.28504381800907197
1111	22	0.12123027741333914
1111	23	-1.87371383767519
1111	24	-0.477502361228062
1111	25	-0.1601115914937452
1112	1	-0.1612021670988284
1112	2	-0.3510443194550952
1112	3	0.013869552624026227
1112	4	0.1419420996793619
1112	5	-0.7682774148273097
1112	6	-0.9517029957074286
1112	7	-1.0383859378610936
1112	8	-0.8014393621272262
1112	9	-0.7628226040815349
1112	10	-0.9016779695599482
1112	11	-0.7500034845330887
1112	12	-0.717808560424821
1112	13	-0.8187640286854148
1112	14	-0.6546176393650285
1112	15	-1.0439876434238684
1112	16	-1.0820380614699374
1112	17	-0.8720360099168261
1112	18	-1.0668131026717305
1112	19	-0.8417576684073325
1112	20	-0.9724930240086493
1112	21	-2.004911904868777
1112	22	-1.7463281059507523
1112	23	-1.3657021637036095
1112	24	-1.7610171790528284
1112	25	-1.991923935834997
1113	1	0.09693669194025745
1113	2	-0.03224652691386609
1113	3	0.04735634023684554
1113	4	0.3252707771940848
1113	5	0.11617079932833008
1113	6	0.03850514191256593
1113	7	0.06296128332859041
1113	8	0.0777241224376084
1113	9	0.29182619325293047
1113	10	-0.06879088142154899
1113	11	-0.1892319102581439
1113	12	-0.07147918528823993
1113	13	-0.05228336948157164
1113	14	0.14301345008901478
1113	15	-0.00924171970569887
1113	16	0.03775596361290923
1113	17	0.10835998998405216
1113	18	0.18428946275941627
1113	19	0.06376150417860771
1113	20	-0.5276560264709715
1113	21	-0.4544901402684687
1113	22	0.07805892173710305
1113	23	-0.11021205243705623
1113	24	0.10219036457481678
1113	25	-0.9162663202298944
1114	1	-0.7991015821156253
1114	2	-0.5679305965196768
1114	3	-0.34799491213791
1114	4	-1.255819331885931
1114	5	-0.5573517789388163
1114	6	-0.36062008515943234
1114	7	-0.3672552975625436
1114	8	-0.35595970713791303
1114	9	-0.30304856555564186
1114	10	-0.5274787331764585
1114	11	-0.49533350995226755
1114	12	-0.45446126067336223
1114	13	-0.4148050920370281
1114	14	-0.45893309895537726
1114	15	-1.1684836104161178
1114	16	-0.7130959395560481
1114	17	-0.7770755219399916
1114	18	-0.6967714413967911
1114	19	-0.6378457326974782
1114	20	-1.933700516791177
1114	21	-6.657979534162262
1114	22	-7.973883298587669
1114	23	-8.022247639342698
1114	24	-5.8287394332535385
1114	25	-7.438489743842116
1115	1	-1.0186470352798906
1115	2	-1.0477279662921148
1115	3	-0.8147558519216247
1115	4	-0.6514317956883957
1115	5	-0.7834705220449578
1115	6	-0.9395827466239837
1115	7	-1.0245953067391724
1115	8	-0.814142249063238
1115	9	-2.7812620558031966
1115	10	-1.7702958205397579
1115	11	-1.2034070414642528
1115	12	-1.0199028326111161
1115	13	-1.4602191340975779
1115	14	-1.166465376672889
1115	15	-2.035953535696507
1115	16	-1.3889339143780575
1115	17	-3.113830718736075
1115	18	-2.833800949414183
1115	19	-2.0909378301641794
1115	20	-1.8289061856365336
1115	21	-1.3190289360103478
1115	22	-0.9805469874088999
1115	23	-0.464561759495723
1115	24	-1.3188073311681379
1115	25	-1.197070800491069
1116	1	-1.462453155521608
1116	2	-0.7519264468938616
1116	3	-0.6190602639141929
1116	4	-0.17335387896995513
1116	5	-0.3379427290537649
1116	6	-0.41910926168833385
1116	7	-0.665970949729676
1116	8	-1.7347289908407078
1116	9	-0.8444578777648246
1116	10	-2.406875402551826
1116	11	-1.1249658437415135
1116	12	-0.670628972735102
1116	13	-0.7964694159932009
1116	14	-1.0035231840695218
1116	15	-1.2087916071775666
1116	16	-0.822209027634905
1116	17	-0.6335754938869158
1116	18	-1.2168108123093546
1116	19	-0.7907021117253663
1116	20	-1.0327823080413694
1116	21	-0.5945251490206545
1116	22	-0.10323640209385587
1116	23	0.04407651970598181
1116	24	-0.054598832724508384
1116	25	-0.11656639524865398
1201	1	0.3111592804179004
1201	2	0.37778255533451244
1201	3	0.3639727614228896
1201	4	0.4853513918464216
1201	5	0.4960752253017304
1201	6	0.3213877525385982
1201	7	0.3363714726106082
1201	8	0.49290289494795303
1201	9	0.7557190076277749
1201	10	0.618384017893348
1201	11	0.4713118858506036
1201	12	0.5609284729706019
1201	13	0.46014398314185456
1201	14	0.9824781964885716
1201	15	0.8526303587841966
1201	16	0.8269998584375458
1201	17	0.7053852445230646
1201	18	0.5626966200056884
1201	19	0.6341103913116584
1201	20	0.735833980902207
1201	21	0.6670755217770363
1201	22	1.0273000030810806
1201	23	0.775227807971703
1201	24	0.6769514017728966
1201	25	0.7117924247136571
1202	1	-0.2501346901352228
1202	2	0.06543445292280836
1202	3	0.013510667770997742
1202	4	-0.11897740583636107
1202	5	0.029071570303123906
1202	6	-0.05839344478754782
1202	7	6.201201332558526E-4
1202	8	0.09426510847968217
1202	9	0.10924189512196136
1202	10	0.35772515917671655
1202	11	0.13797064991357644
1202	12	0.24393177492704154
1202	13	0.19506126463552675
1202	14	0.33184555754547773
1202	15	0.3650936083472658
1202	16	0.371835280500072
1202	17	0.26462000965723576
1202	18	0.1787747247233611
1202	19	0.18647921452784288
1202	20	0.3123466790988445
1202	21	0.3865758001884245
1202	22	0.3859368518921116
1202	23	0.6093008338902024
1202	24	0.2667989889614759
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2211	4	0.3124964568534381
2211	5	0.26851264485007675
2211	6	0.12267268782319007
2211	7	0.170555146079991
2211	8	0.27556470431346014
2211	9	0.4263280657029327
2211	10	0.35659589786884993
2211	11	0.13350502397541675
2211	12	0.18809155498233565
2211	13	0.062429539127869305
2211	14	0.5335501561971299
2211	15	0.3125701215845993
2211	16	0.13072802453558552
2211	17	0.2954629780047
2211	18	0.2979435050426247
2211	19	0.22712031420304868
2211	20	0.2935982684910954
2211	21	0.3103351832656358
2211	22	0.4239875819705716
2211	23	0.4872405677602458
2211	24	0.39178804065380035
2211	25	0.5909821593950397
2212	1	-0.13019752159665288
2212	2	-0.1722798878076297
2212	3	-0.042641241530919416
2212	4	-0.349135056554303
2212	5	-0.19694083937211815
2212	6	-0.5028826762116552
2212	7	-0.37279819196032804
2212	8	-0.25817286663667716
2212	9	-0.10021518611991809
2212	10	-0.5302954667935893
2212	11	-0.35654165367422763
2212	12	-0.5479014209954371
2212	13	-0.5121814988738862
2212	14	-0.22621394736990652
2212	15	-0.4937339300729562
2212	16	-0.12504853487580192
2212	17	-0.25320713161513453
2212	18	-0.2103553269388314
2212	19	-0.23362977115257877
2212	20	-0.28237003446226083
2212	21	-0.24931693592949244
2212	22	-0.4202433721230179
2212	23	0.17790445774485875
2212	24	-0.12732747617037177
2212	25	-0.7040235972756861
2213	1	-0.0532412793818402
2213	2	-1.35041613029032
2213	3	-0.22824255948808414
2213	4	0.02671150322925797
2213	5	0.03444408716248404
2213	6	0.017776972065220775
2213	7	0.12729730635874148
2213	8	0.17478637580583012
2213	9	0.057438876911653836
2213	10	-0.3443276924535752
2213	11	-0.027592537000195705
2213	12	0.02241922833955101
2213	13	-0.3515087246008406
2213	14	-0.05139978736669093
2213	15	-0.0037688880831581173
2213	16	-0.002374983758080873
2213	17	0.08379933762584102
2213	18	-0.025304765065833124
2213	19	0.07334816913753173
2213	20	0.2290458319204518
2213	21	-0.30676473330697007
2213	22	-0.7138673525143915
2213	23	-0.08218059335277417
2213	24	0.12667099692802708
2213	25	-0.33438737488380355
2214	1	-0.2451685006998403
2214	2	-0.4013803349640277
2214	3	-0.37454553074965347
2214	4	-0.4893737022404352
2214	5	-0.3148008013409509
2214	6	-0.34148561807650424
2214	7	-0.585663975762607
2214	8	-0.20473330390143474
2214	9	-0.12006733054204233
2214	10	-1.919318722831484
2214	11	-0.9297560573689552
2214	12	-0.6161825798412153
2214	13	-0.3686765812769721
2214	14	-0.9406267366259513
2214	15	-0.6414560324168548
2214	16	-0.6590640645797237
2214	17	-0.5851066060853256
2214	18	-0.5082396729341525
2214	19	-1.5231321749157871
2214	20	-2.368714257419356
2214	21	-1.7076209461068004
2214	22	-2.1273469718697626
2214	23	-2.6365622834434177
2214	24	-1.781120575191295
2214	25	-2.0408743719018503
2215	1	-0.1939993349306839
2215	2	-0.3683143149213244
2215	3	-0.8331285852561727
2215	4	-0.27610751584299464
2215	5	-0.18442076332942747
2215	6	-0.2587979995318648
2215	7	-0.15892595260328335
2215	8	-0.15855936617487743
2215	9	-0.23882015763112463
2215	10	-0.2799717737704981
2215	11	-0.46349695818513936
2215	12	-0.31981988790690963
2215	13	-0.2360710780539108
2215	14	-0.13611176976897355
2215	15	-0.6647377294861414
2215	16	-0.49529770366912373
2215	17	-0.16092400594060052
2215	18	-0.24534247446175783
2215	19	-1.9992694914295583
2215	20	-2.2643557489734505
2215	21	-3.1200865443248844
2215	22	-2.1093980639166503
2215	23	-3.197615097450047
2215	24	-2.29697037704765
2215	25	-1.773705221580606
2216	1	-1.2431396683436233
2216	2	-1.0386372181754808
2216	3	-0.7196500760687776
2216	4	-0.7582582111694336
2216	5	-1.7221682990792857
2216	6	-1.277652747965213
2216	7	-0.9368903745011181
2216	8	-4.956593041967711
2216	9	-4.0567274153039605
2216	10	-1.808853936979098
2216	11	-0.8872822518781831
2216	12	-0.982908221618807
2216	13	-1.7202860370607738
2216	14	-3.351211064035091
2216	15	-1.857860469824331
2216	16	-2.725514441313301
2216	17	-2.347081431167112
2216	18	-2.3375134271671953
2216	19	-1.3892203853615754
2216	20	-0.6903562446353178
2216	21	-0.6955491154028195
2216	22	-0.5581450813638966
2216	23	-0.8806557687456475
2216	24	-0.5131237439817937
2216	25	-0.142533093260667
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	46.0
8	170.0
9	427.0
10	941.0
11	1760.0
12	2903.0
13	4475.0
14	6004.0
15	7852.0
16	8975.0
17	9717.0
18	10259.0
19	10570.0
20	10704.0
21	11742.0
22	12357.0
23	13327.0
24	14376.0
25	15569.0
26	17638.0
27	19829.0
28	22864.0
29	27293.0
30	33456.0
31	41166.0
32	56302.0
33	82418.0
34	123699.0
35	201841.0
36	363833.0
37	1242429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.95117112639573	20.706087498578473	12.73038189545154	23.61235947957426
2	15.954489981041	20.70615742117192	39.729714250580514	23.60963834720656
3	18.968468921868677	25.062846106320997	29.46285267626074	26.50583229554958
4	12.51693569342952	16.65086633679003	38.005654426359584	32.82654354342087
5	13.30056398046979	37.79654670938391	35.42434149984017	13.478547810306132
6	31.87588698775799	38.27895949229558	16.820488417045325	13.024665102901109
7	26.865255597208964	32.51701447941356	22.57406556685386	18.04366435652361
8	24.217837457734884	37.28341102114203	20.708832830152133	17.78991869097095
9	24.020720396756353	16.96867770620391	21.919794783519688	37.09080711352005
10	14.453876836751494	30.355427756722037	34.02183574724632	21.16885965928015
11	32.96331135911178	25.212345425330877	22.850102572567145	18.9742406429902
12	20.67362758044521	27.318989853392985	31.430226917479654	20.577155648682155
13	26.97785699578211	23.518778414581615	26.395259383522795	23.108105206113486
14	20.21802080162162	23.23084949288626	27.985427664447215	28.565702041044904
15	22.843651666208398	30.88729002649815	23.790603579207904	22.478454728085552
16	21.93143424026733	30.941248597828615	25.591985811541427	21.535331350362625
17	20.283230198645157	30.04364837415491	28.30154516760369	21.371576259596242
18	20.711754171661628	29.69607472841934	29.024170773167462	20.568000326751562
19	21.148016027406257	28.77602390300017	29.152371520531954	20.923588549061613
20	22.169121331371734	28.601679036133216	28.60500544436182	20.624194188133227
21	22.371868377125168	28.640234376810785	27.979068923622307	21.00882832244174
22	21.37438036067297	28.481811481306202	28.890626995140657	21.253181162880175
23	21.58197314058294	28.694265529628126	28.518998309276057	21.204763020512882
24	21.47581827207986	28.29804712846323	28.649470527973026	21.57666407148388
25	21.870633067285002	28.311799470660752	28.070975858973423	21.74659160308082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1687.0
1	1687.0
2	1853.0
3	2019.0
4	2019.0
5	2019.0
6	3216.5
7	4414.0
8	4414.0
9	4414.0
10	7080.0
11	9746.0
12	9746.0
13	9746.0
14	16360.0
15	22974.0
16	22974.0
17	22974.0
18	35991.0
19	49008.0
20	49008.0
21	49008.0
22	68962.5
23	88917.0
24	88917.0
25	88917.0
26	114452.5
27	139988.0
28	139988.0
29	139988.0
30	166520.5
31	193053.0
32	193053.0
33	193053.0
34	213970.0
35	234887.0
36	234887.0
37	234887.0
38	249488.5
39	264090.0
40	264090.0
41	264090.0
42	264059.5
43	264029.0
44	264029.0
45	264029.0
46	265605.0
47	267181.0
48	267181.0
49	267181.0
50	256189.5
51	245198.0
52	245198.0
53	245198.0
54	226253.5
55	207309.0
56	207309.0
57	207309.0
58	182926.0
59	158543.0
60	158543.0
61	158543.0
62	134397.0
63	110251.0
64	110251.0
65	110251.0
66	86650.5
67	63050.0
68	63050.0
69	63050.0
70	46412.0
71	29774.0
72	29774.0
73	29774.0
74	21204.5
75	12635.0
76	12635.0
77	12635.0
78	8411.5
79	4188.0
80	4188.0
81	4188.0
82	2792.0
83	1396.0
84	1396.0
85	1396.0
86	914.5
87	433.0
88	433.0
89	433.0
90	280.5
91	128.0
92	128.0
93	128.0
94	80.5
95	33.0
96	33.0
97	33.0
98	23.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030905991888650848
2	0.014568764568764568
3	0.020758384197690556
4	0.02193735936510503
5	0.024042672164059448
6	0.014063489497015509
7	0.002315844078849859
8	0.0022737378228707707
9	0.0036211380142015977
10	0.001305293935351739
11	0.0016842502391635338
12	0.006737000956654135
13	0.002652694126682566
14	0.0013895064473099154
15	0.0025684816147243895
16	0.0023579503348289477
17	0.0023579503348289477
18	0.002147419054933506
19	5.052750717490602E-4
20	4.2106255979088345E-4
21	0.08475989328590484
22	0.0838335556543649
23	0.08404408693426034
24	0.001305293935351739
25	0.23474237708341755
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2374944.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.46467689714578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.83459222768431	70.59212168662631
2	8.366095715995241	13.296181859243145
3	1.4659192760260134	3.49466404880115
4	0.4566692280449943	1.4515629062185782
5	0.21939534100578756	0.8717089942882013
6	0.1379063749617551	0.6575211317035501
7	0.08814305767230017	0.49029817190590735
8	0.05997231135780034	0.3812544275858104
9	0.04849998833101892	0.3468632312015783
>10	0.2883974595506	4.326507244287776
>50	0.023554294857321675	1.2769063900615072
>100	0.00952760591998572	1.3332586400688335
>500	4.7776269341617616E-4	0.2658833573209936
>1k	8.493558994062686E-4	1.2152679106867263
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3855	0.16231961679938559	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3303	0.1390769634989288	No Hit
CCCATGTACTCTGCGTTGATACCAC	3301	0.13899275098697064	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2528	0.10644461511513535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.210625597908835E-5	0.0
6	0.0	0.0	0.0	4.210625597908835E-5	0.0
7	0.0	0.0	0.0	4.210625597908835E-5	0.0
8	0.0	0.0	0.0	4.210625597908835E-5	0.0
9	0.0	0.0	0.0	4.210625597908835E-5	0.0
10	0.0	0.0	0.0	8.42125119581767E-5	0.0
11	0.0	0.0	0.0	8.42125119581767E-5	0.0
12	0.0	0.0	0.0	1.2631876793726504E-4	0.0
13	0.0	0.0	0.0	1.684250239163534E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAC	40	0.005278987	14.250489	3
ACTATCG	160	0.0	12.463927	11
CTATCGG	170	1.8189894E-12	11.730755	12
ATCGGTC	185	5.456968E-12	10.778705	14
GTACTGG	490	0.0	10.665429	1
GTATTAG	320	0.0	10.392734	1
TATCGGT	195	1.4551915E-11	10.225736	13
GCACCGT	75	0.0026560351	10.129841	6
TTAGACT	195	1.6370905E-10	9.74372	4
CGGTCAG	205	4.1836756E-11	9.735529	16
TCGGTCA	205	4.1836756E-11	9.732452	15
CTAGTAC	180	4.1818566E-9	9.500327	3
CGTATCC	80	0.004515652	9.500326	3
TAGAACC	130	4.2395295E-6	9.500127	4
AGCACCG	90	0.0011146922	9.499326	5
ACACCGT	80	0.004529795	9.496725	6
GACCGTC	80	0.0045313686	9.4963255	11
TATACTG	520	0.0	9.316647	5
ATTAGAC	155	4.259873E-7	9.193864	3
CTAGGGC	155	4.259873E-7	9.193864	3
>>END_MODULE