FastQCFastQC Report
Wed 25 May 2016
SRR1294764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374944
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93910.3954198498996187No Hit
GGTATCAACGCAGAGTACTTTTTTT80050.33706057911260223No Hit
TATCAACGCAGAGTACTTTTTTTTT60460.25457442364956817No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40990.17259354325828313No Hit
GTACATGGGAAGCAGTGGTATCAAC40500.17053033671530782No Hit
CCCATGTACTCTGCGTTGATACCAC33940.14290863279302587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGAA604.114237E-412.6590612
TGTACCG657.926731E-411.7096935
TCCCGCA951.338378E-511.0165892
CTACACC2350.010.9311244
TGGACCG700.001473525910.8732855
AGGACCG700.001473525910.8732855
GTCTCGC1105.9047998E-610.3845241
CCTACAC2750.010.3790463
TCCGAAT750.002661684810.12724713
GTAGCAC2201.8189894E-129.9465863
CTAGTAC1356.316259E-79.8665013
GGACCGT800.00453464139.4954946
GTCTAGG2550.09.3324971
GTACTAG1451.6608446E-69.19091
CGAGGCG1054.5207358E-49.04218512
AAACCGC850.0074661678.9369356
CTAGCAC2605.456968E-128.78226953
CTAGGAC3150.08.7590363
GTGCTAG2303.8926373E-108.6913951
AGGACAG5500.08.6492055
Produced by FastQC (version 0.11.5)