FastQCFastQC Report
Wed 25 May 2016
SRR1294763_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294763_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588597
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1033302.879398271803716No Hit
CCCATGTACTCTGCGTTGATACCAC996272.7762103128325637No Hit
GAGTACATGGGAAGCAGTGGTATCA508141.4159851329084876No Hit
CATGTACTCTGCGTTGATACCACTG418281.1655808662828397No Hit
GCGTTGATACCACTGCTTCCCATGT332290.925960758480264No Hit
GCTTCCCATGTACTCTGCGTTGATA286490.7983342793855092No Hit
ACGCAGAGTACATGGGAAGCAGTGG272490.7593218185268505No Hit
GTATCAACGCAGAGTACATGGGAAG263570.7344653077511908No Hit
ACTCTGCGTTGATACCACTGCTTCC221650.6176508535229784No Hit
TATCAACGCAGAGTACATGGGAAGC203990.5684394207541276No Hit
GGTATCAACGCAGAGTACATGGGAA180360.5025919600334058No Hit
CAGTGGTATCAACGCAGAGTACATG148280.4131976925801365No Hit
GTGGTATCAACGCAGAGTACATGGG139210.38792319115241974No Hit
GCAGTGGTATCAACGCAGAGTACAT123430.3439505745560173No Hit
GTACATGGTAAGCAGTGGTATCAAC111510.31073425073921646No Hit
ATACCACTGCTTCCCATGTACTCTG111330.31023266195674803No Hit
GTACTCTGCGTTGATACCACTGCTT105460.2938752944395818No Hit
ACATGGGAAGCAGTGGTATCAACGC102740.28629573061561386No Hit
CATGGGAAGCAGTGGTATCAACGCA102660.2860728022678501No Hit
CTGCTTCCCATGTACTCTGCGTTGA101020.28150277113869293No Hit
GGGAAGCAGTGGTATCAACGCAGAG94570.26352917310023943No Hit
GTTGATACCACTGCTTCCCATGTAC86640.24143140062815638No Hit
AAGCAGTGGTATCAACGCAGAGTAC85390.23794814519434754No Hit
TACCACTGCTTCCCATGTACTCTGC85250.23755802058576095No Hit
CTTCCCATGTACTCTGCGTTGATAC84490.2354402012820052No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA82540.23000632280526348No Hit
CCACTGCTTCCCATGTACTCTGCGT81550.2272475845016869No Hit
GCAGAGTACATGGGAAGCAGTGGTA81040.22582641628469288No Hit
GATACCACTGCTTCCCATGTACTCT81040.22582641628469288No Hit
ACCATGTACTCTGCGTTGATACCAC80550.2244609801546398No Hit
CCATGTACTCTGCGTTGATACCACT75630.21075088676716833No Hit
GTACATGGGTAAGCAGTGGTATCAA72700.20258613603032047No Hit
ATGGGAAGCAGTGGTATCAACGCAG67020.18675822333909325No Hit
TTCCCATGTACTCTGCGTTGATACC60800.1694255443004606No Hit
TGATACCACTGCTTCCCATGTACTC58290.16243116738937252No Hit
GGTAAGCAGTGGTATCAACGCAGAG52960.1475785662196117No Hit
CCCCATGTACTCTGCGTTGATACCA49060.13671080926612825No Hit
GCTTACCATGTACTCTGCGTTGATA43680.12171887787901511No Hit
GAGTACATGGTAAGCAGTGGTATCA38950.10853823931748258No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA38940.1085103732740121No Hit
ATCAACGCAGAGTACATGGGAAGCA38540.10739573153519329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCT500.00150529813.294039
GTCGTAG604.0920413E-412.6675121
AATACCG550.003075952612.0876725
TATCGAC1156.866685E-811.59119919
CCGGTCA600.00589787411.0783599
CGCAAAA3100.011.0314572
CGCTGCG951.3682498E-510.9950639
AAGCGGT1502.1518645E-910.76183410
GATACGA750.00263441210.14008818
GTATAGA4550.010.0226471
GTACGCT1054.1260668E-59.9484686
TACGCTG1054.129226E-59.9477757
GTACAAA7850.09.5611491
GTCCTAG2900.09.5006341
ATGTACG1106.856229E-59.4981184
GAAGCGG1804.2200554E-99.4957369
GAACAAA15300.09.3764421
TTAGACA3550.09.3643424
GCCTTAC1856.699338E-99.2438611
GGTATCG1752.6442649E-89.23472417
Produced by FastQC (version 0.11.5)