##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294763_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3588597
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99174524194274	33.0	33.0	33.0	27.0	33.0
2	30.99280944614288	33.0	33.0	33.0	27.0	33.0
3	31.014255710518622	33.0	33.0	33.0	27.0	33.0
4	34.34297414839281	37.0	37.0	37.0	27.0	37.0
5	34.75551921823487	37.0	37.0	37.0	27.0	37.0
6	34.78650709455534	37.0	37.0	37.0	27.0	37.0
7	34.8634042774934	37.0	37.0	37.0	33.0	37.0
8	34.7558310392613	37.0	37.0	37.0	27.0	37.0
9	34.469288136840106	37.0	37.0	37.0	27.0	37.0
10	34.6043863381706	37.0	37.0	37.0	27.0	37.0
11	34.839173359393655	37.0	37.0	37.0	33.0	37.0
12	34.7846648704215	37.0	37.0	37.0	27.0	37.0
13	34.6495368524245	37.0	37.0	37.0	27.0	37.0
14	35.90971123255133	40.0	37.0	40.0	27.0	40.0
15	36.14016229741038	40.0	37.0	40.0	27.0	40.0
16	36.12894760821569	40.0	37.0	40.0	27.0	40.0
17	36.11884226621156	40.0	37.0	40.0	27.0	40.0
18	36.10711567779831	40.0	37.0	40.0	27.0	40.0
19	36.1282924775337	40.0	37.0	40.0	27.0	40.0
20	36.054583727289526	40.0	37.0	40.0	27.0	40.0
21	35.980218453061184	40.0	37.0	40.0	27.0	40.0
22	35.8840279362659	40.0	37.0	40.0	27.0	40.0
23	35.53600501811711	37.0	37.0	40.0	27.0	40.0
24	35.877325038169516	40.0	37.0	40.0	27.0	40.0
25	34.821489846867735	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.35709070532260156
1101	2	0.5151570107772834
1101	3	0.3678769466239622
1101	4	0.41343021824851434
1101	5	0.3192684843617499
1101	6	0.2780191802895047
1101	7	0.2961094889165281
1101	8	0.3671401707963895
1101	9	0.5610257111265398
1101	10	0.609241757880298
1101	11	0.3683861374344417
1101	12	0.4379428532326983
1101	13	0.4063705861918194
1101	14	0.9986659642935081
1101	15	0.8236554277802242
1101	16	0.8588014846876035
1101	17	0.8770547715658523
1101	18	0.8175392633129093
1101	19	0.7093972418643801
1101	20	0.830531268455097
1101	21	0.812719244190923
1101	22	0.9092476320507856
1101	23	1.2082535795351816
1101	24	0.9670577108976488
1101	25	0.7780460781962475
1102	1	0.2785661660269234
1102	2	0.4726631294611394
1102	3	0.3029871265551449
1102	4	0.2529424335145123
1102	5	0.3022934261411905
1102	6	0.26895515279963433
1102	7	0.3158421716453219
1102	8	0.4076964446575104
1102	9	0.6643307746282403
1102	10	0.634445122691119
1102	11	0.39721541859378107
1102	12	0.40656896733335657
1102	13	0.40336577150884523
1102	14	1.1033014198562725
1102	15	0.8160775221615069
1102	16	0.6628464174635127
1102	17	0.7625136786711977
1102	18	0.7417275634820442
1102	19	0.8129483322929403
1102	20	0.8947653075309248
1102	21	0.8427591028795689
1102	22	0.8639218964709912
1102	23	1.133696806059092
1102	24	0.8865610302254723
1102	25	0.808443417499376
1103	1	0.20337039458541994
1103	2	0.4327090843876391
1103	3	0.2068417198295336
1103	4	0.027733155404213505
1103	5	0.3037428217885392
1103	6	0.17176107096391036
1103	7	0.10241447869544373
1103	8	0.32906000253984047
1103	9	0.5396501738384245
1103	10	0.4375911050179866
1103	11	0.4215421869478533
1103	12	0.317660719722042
1103	13	0.2925971829245526
1103	14	0.9706892258106308
1103	15	0.6279149688112398
1103	16	0.4555499422489433
1103	17	0.45040402014515735
1103	18	0.556926518612741
1103	19	0.63710673494964
1103	20	0.7585540467462835
1103	21	0.7714203114042562
1103	22	0.6599316344602499
1103	23	1.0817246926535375
1103	24	0.7732104424721555
1103	25	0.5726539274436675
1104	1	0.19922368189599382
1104	2	0.4453228507702498
1104	3	0.2835891634111469
1104	4	0.20127180632979957
1104	5	0.11026801022327248
1104	6	0.11591177893371452
1104	7	0.16566485350360693
1104	8	0.342211327248954
1104	9	0.5361646628152243
1104	10	0.5070444357778499
1104	11	0.2812716496923926
1104	12	0.3323313273574726
1104	13	0.3772593831646418
1104	14	0.7137998734438895
1104	15	0.5792480708981103
1104	16	0.4435280458049391
1104	17	0.5401945873226595
1104	18	0.49324713835044776
1104	19	0.6040562546629786
1104	20	0.6181569465501866
1104	21	0.6240413198041637
1104	22	0.7808164437872733
1104	23	0.9759093858332051
1104	24	0.7748909366149661
1104	25	0.6844283859517972
1105	1	0.13014217099386372
1105	2	0.2278455922127982
1105	3	0.1564971686465242
1105	4	0.1638998857576368
1105	5	0.11256309120131647
1105	6	0.05414995447073778
1105	7	0.08205102009834064
1105	8	0.09229312351471464
1105	9	-1.7686278337129195
1105	10	-0.23790747321955052
1105	11	-0.08954679255923281
1105	12	0.09938170043687222
1105	13	0.01247893578562298
1105	14	-1.9087280197329406
1105	15	-0.4998600756770486
1105	16	-0.2057067832895001
1105	17	0.0902395973759269
1105	18	-0.08124020843045088
1105	19	0.11449543480899393
1105	20	0.2774139378811853
1105	21	0.39072455899246705
1105	22	0.41081455974228476
1105	23	0.6692731052438674
1105	24	0.4276166066603224
1105	25	0.23880451477927522
1106	1	0.049912179155370495
1106	2	0.020232977827024712
1106	3	0.12963200149305365
1106	4	-1.0159302184000083
1106	5	-0.23441901383808528
1106	6	-0.0887465675718957
1106	7	0.04208859977801893
1106	8	0.1738567740971746
1106	9	0.35483684479974187
1106	10	0.3130059071933502
1106	11	0.27386120152038984
1106	12	0.14962237309214288
1106	13	0.19130383599740242
1106	14	0.5696012590888628
1106	15	0.34420818707629053
1106	16	0.10689957219012314
1106	17	0.04380633411085455
1106	18	0.17922108738618192
1106	19	0.11620961405251506
1106	20	0.3454542573084609
1106	21	0.4913298752574846
1106	22	0.49623411639653625
1106	23	0.6816867861367513
1106	24	0.3113095974727216
1106	25	0.26915955352676235
1107	1	0.24426436190360334
1107	2	0.2162753014203247
1107	3	0.20219033657819097
1107	4	0.3098745605222817
1107	5	0.24143271344101436
1107	6	0.04846596133288017
1107	7	0.1469782361034362
1107	8	0.11175881319744718
1107	9	0.2743070991617387
1107	10	0.3026212109562252
1107	11	0.08911731531760125
1107	12	0.10949344029135943
1107	13	0.10522437415170316
1107	14	0.5115002150134629
1107	15	0.3642936751289838
1107	16	0.44292928279204347
1107	17	0.3764501572486836
1107	18	0.37585762301788606
1107	19	0.40514521772429646
1107	20	0.4183489388304338
1107	21	0.4495612535771514
1107	22	0.51472195887429
1107	23	0.7299693171436914
1107	24	0.5625744002195532
1107	25	0.504391842784699
1108	1	0.5095210708036397
1108	2	0.5222784441311887
1108	3	0.4830201440705224
1108	4	0.714884192892967
1108	5	0.6799036897730844
1108	6	0.4934551818510542
1108	7	0.46440070203964723
1108	8	0.5912243281595551
1108	9	0.3302396777233412
1108	10	0.5488946130509618
1108	11	0.3947682911728947
1108	12	0.4167573216625797
1108	13	0.5364457575587664
1108	14	-0.8111105934936305
1108	15	0.23430423677178425
1108	16	0.5181862375549926
1108	17	0.6321732890100549
1108	18	0.782061521387277
1108	19	0.7093012125831493
1108	20	0.8148400566405414
1108	21	0.8340009413506877
1108	22	0.9471699291638842
1108	23	1.264491570763461
1108	24	1.0917767110917467
1108	25	0.9455914116502626
1109	1	0.4030408275891837
1109	2	0.21065302899825156
1109	3	0.38818976784424564
1109	4	0.46396445830217914
1109	5	0.4608454122244652
1109	6	0.27650220903838374
1109	7	0.23972031600128219
1109	8	0.31838319186302755
1109	9	0.463881731403319
1109	10	0.4480085154353972
1109	11	0.29018482225716014
1109	12	0.22411475950941195
1109	13	0.21693217392621023
1109	14	0.6092683977231346
1109	15	0.39210246633206935
1109	16	0.369620589652925
1109	17	0.27237577545025005
1109	18	0.37429523598145664
1109	19	0.42679162463452514
1109	20	0.6233602115524448
1109	21	0.594314200964817
1109	22	0.6309232387851864
1109	23	1.0176179285467697
1109	24	0.7234192926810081
1109	25	0.6207307088537846
1110	1	0.38088079788529683
1110	2	0.28960782339164126
1110	3	0.40404514703296
1110	4	0.5908056353187945
1110	5	0.4553882508023932
1110	6	0.3166006031718638
1110	7	0.3329756766250682
1110	8	0.29036035699809304
1110	9	0.45866684232877475
1110	10	0.5344217707960226
1110	11	0.25363140166864895
1110	12	0.2587550639742773
1110	13	0.20407131808290302
1110	14	0.5483336534550602
1110	15	0.3679764104516323
1110	16	0.2679423199044848
1110	17	0.33072256835844627
1110	18	0.5200735707071615
1110	19	0.4000692631753253
1110	20	0.5508483764090357
1110	21	0.5114979009555327
1110	22	0.5387297922057783
1110	23	0.8129395659736716
1110	24	0.7359896073565864
1110	25	0.5214723778410715
1111	1	0.184102405494329
1111	2	0.058335208973179675
1111	3	0.22873817512869365
1111	4	0.44731219829230895
1111	5	0.3899576868795762
1111	6	0.2706393420586224
1111	7	0.22844348245597956
1111	8	0.3090636448743851
1111	9	0.290291794407203
1111	10	0.24894118587899072
1111	11	0.2020935299341602
1111	12	0.16449615968963371
1111	13	0.035839213367417244
1111	14	-1.6932376264616948
1111	15	-0.47953458869034904
1111	16	-0.0979152480528711
1111	17	0.02770798194443813
1111	18	0.23222045717410822
1111	19	0.2978606719782846
1111	20	0.38121385827185605
1111	21	0.4023666962528267
1111	22	0.20397067797900093
1111	23	-1.7211148729331711
1111	24	-0.2599170688031194
1111	25	0.06206404952808953
1112	1	-0.11311116093047957
1112	2	-0.1710483563300187
1112	3	0.0370826626172267
1112	4	0.23773119471201198
1112	5	-0.696960514684946
1112	6	-0.8059929770043439
1112	7	-0.9439486300614064
1112	8	-0.7192388961500811
1112	9	-0.6580137234174472
1112	10	-0.8383352577835907
1112	11	-0.8624455383863534
1112	12	-0.7393140973683856
1112	13	-0.7522793085687312
1112	14	-0.564207432343423
1112	15	-0.799453273925927
1112	16	-0.8092302613275848
1112	17	-0.8690372102107276
1112	18	-1.0178245663389518
1112	19	-0.9151629349670927
1112	20	-0.9445832407160637
1112	21	-1.809897614354334
1112	22	-1.729773581154518
1112	23	-1.2837837111071622
1112	24	-1.461506649436366
1112	25	-1.7083057141076097
1113	1	0.12198173180922112
1113	2	0.0029201147028175
1113	3	0.14241627643550103
1113	4	0.3947752687927064
1113	5	0.14553690060596836
1113	6	0.11725320056489608
1113	7	0.016052821865116584
1113	8	0.20520420692190555
1113	9	0.40475671003516567
1113	10	0.03543346768480404
1113	11	0.018712048018272753
1113	12	0.062483509900111756
1113	13	-0.06715342191256468
1113	14	0.03627530752303443
1113	15	-0.05654942201968538
1113	16	0.02881081905425731
1113	17	0.1020090787718857
1113	18	0.12146490811333166
1113	19	-0.013719273343191674
1113	20	-0.5701715722531517
1113	21	-0.4687294943427247
1113	22	0.04438284639340395
1113	23	-0.2421931844308105
1113	24	0.04837687487023601
1113	25	-1.1446571278482338
1114	1	-0.8700772453400631
1114	2	-0.6197365471982792
1114	3	-0.5096291837456697
1114	4	-1.4721776977222376
1114	5	-0.6582211893257579
1114	6	-0.5237299299252882
1114	7	-0.415914225571278
1114	8	-0.35992633106495475
1114	9	-0.37248756818935647
1114	10	-0.40754827427667806
1114	11	-0.5135788849609568
1114	12	-0.4673363558448429
1114	13	-0.5031826966607227
1114	14	-0.53613263555971
1114	15	-1.3331232814639336
1114	16	-0.7992006792127739
1114	17	-0.7843526989501157
1114	18	-0.6443156679676676
1114	19	-0.6909435571126537
1114	20	-1.8809572205557217
1114	21	-6.566596418481847
1114	22	-7.4804778403377625
1114	23	-7.5934302405820375
1114	24	-5.759774845428158
1114	25	-7.122268655849457
1115	1	-0.864956439186443
1115	2	-0.9315660537094885
1115	3	-0.6422201234731482
1115	4	-0.4847284879474856
1115	5	-0.5910239839730309
1115	6	-0.7266179143260629
1115	7	-0.8763161393021903
1115	8	-0.6371945249712283
1115	9	-2.42415487164201
1115	10	-1.481116945103679
1115	11	-1.0078446707112505
1115	12	-0.9259900448711349
1115	13	-1.2957403839470416
1115	14	-1.0014918264751316
1115	15	-1.8736980636918474
1115	16	-1.2248178527785356
1115	17	-2.9928679524461828
1115	18	-2.6883796303583267
1115	19	-1.8504570526141535
1115	20	-1.6992636838463184
1115	21	-1.2105908287220615
1115	22	-0.695779255282055
1115	23	-0.2253721819826069
1115	24	-1.0686746711407054
1115	25	-0.7175842952552856
1116	1	-1.3215109874388205
1116	2	-0.5621729104943576
1116	3	-0.3891538761660627
1116	4	-0.029234758706557784
1116	5	-0.05701996754567773
1116	6	-0.07970260907119808
1116	7	-0.6544421353040235
1116	8	-1.5812737649367818
1116	9	-0.6279239937357417
1116	10	-2.0859411327999666
1116	11	-0.8038574670232279
1116	12	-0.530142270938093
1116	13	-0.47437158404421353
1116	14	-0.9329065349279873
1116	15	-0.9172798620978782
1116	16	-0.5661353651506431
1116	17	-0.39482968223139636
1116	18	-1.0086863327143973
1116	19	-0.6432182596610687
1116	20	-0.7537140369503703
1116	21	-0.2111822426268688
1116	22	0.1291518179661466
1116	23	0.28377875185044843
1116	24	0.11699450054192795
1116	25	0.15430436623696409
1201	1	0.15042986065629194
1201	2	0.28158234143580074
1201	3	0.21243551458682575
1201	4	0.2506068493805671
1201	5	0.19603797396962364
1201	6	-0.005563361873825556
1201	7	0.09486747837277676
1201	8	0.1821307525687672
1201	9	0.5166523002702093
1201	10	0.3482760833835741
1201	11	0.24389422602992994
1201	12	0.24702736068564235
1201	13	0.2067662683864171
1201	14	0.8535934760152628
1201	15	0.6407247261076918
1201	16	0.5298367899198269
1201	17	0.48526262572620027
1201	18	0.46875678063311454
1201	19	0.4875657610740589
1201	20	0.5184816230236677
1201	21	0.5419801903291557
1201	22	0.6068806827400977
1201	23	0.46789253775673956
1201	24	0.5229324397226733
1201	25	0.4425730382373061
1202	1	-0.3594104869395167
1202	2	-0.17369563029490465
1202	3	-0.12396309908185188
1202	4	-0.27668130276857283
1202	5	-0.15736635216554618
1202	6	-0.1998552367132902
1202	7	-0.18831999833779633
1202	8	-0.14864493453712413
1202	9	-0.029674753811903543
1202	10	0.16386290455334063
1202	11	-0.06904691639100946
1202	12	-0.16568314858852773
1202	13	0.03270987451202956
1202	14	0.1559613096555026
1202	15	0.18116168095424
1202	16	0.10859900443216475
1202	17	-0.06161939501811986
1202	18	-0.17368225081619926
1202	19	-0.08975296127257337
1202	20	0.035137858439512115
1202	21	0.0783735582242997
1202	22	0.008332676676708672
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2211	3	0.37195087664063564
2211	4	0.4345191103696777
2211	5	0.4365164947823672
2211	6	0.2263571282695409
2211	7	0.3210104969527663
2211	8	0.448546402127846
2211	9	0.48267741915186946
2211	10	0.4291857977134441
2211	11	0.19922627369206225
2211	12	0.3036557577069203
2211	13	0.22420219141356057
2211	14	0.6020028603418766
2211	15	0.3456342086303934
2211	16	0.3042633086591806
2211	17	0.41305050929461373
2211	18	0.3756504680733528
2211	19	0.3128791031860203
2211	20	0.4098146107926226
2211	21	0.5132956637791892
2211	22	0.5821051654398772
2211	23	0.5816502928331531
2211	24	0.6674381270470064
2211	25	0.6720909437308151
2212	1	-0.18704653339627697
2212	2	-0.19151363309932634
2212	3	-0.1421700022391832
2212	4	-0.4500411708157088
2212	5	-0.17497898853948612
2212	6	-0.47134620685839224
2212	7	-0.4402314344167877
2212	8	-0.19952809470110822
2212	9	-0.2580476122013309
2212	10	-0.4602679327374233
2212	11	-0.41566087046245315
2212	12	-0.43800977558576193
2212	13	-0.6218438584594921
2212	14	-0.3446962009737575
2212	15	-0.46699002117146904
2212	16	-0.2767488120705366
2212	17	-0.21279204719598255
2212	18	-0.17759910839993154
2212	19	-0.40267762996425915
2212	20	-0.3786725762316365
2212	21	-0.30963303519313
2212	22	-0.3843758994845885
2212	23	0.09009099376594776
2212	24	-0.1582307881918723
2212	25	-0.86076767825773
2213	1	-0.14993743025767614
2213	2	-1.223758996146831
2213	3	-0.27195761921877093
2213	4	0.03244645937062529
2213	5	0.03012582086863347
2213	6	0.048475102698695594
2213	7	0.07461504475493541
2213	8	0.1296185647581538
2213	9	0.10622016547065982
2213	10	-0.2540538789091542
2213	11	-0.08849206051377934
2213	12	-0.0870074751536265
2213	13	-0.3910841015866069
2213	14	0.0039214819474509
2213	15	-0.17859780517041202
2213	16	-0.09049547582966966
2213	17	0.03519588858848266
2213	18	0.07831991270322902
2213	19	0.09385830008294249
2213	20	0.22431347067998075
2213	21	-0.43566523510037314
2213	22	-0.8872977247193177
2213	23	-0.022889217603527356
2213	24	0.06616359003111683
2213	25	-0.5540091683480526
2214	1	-0.09554150163634034
2214	2	-0.2869618305672361
2214	3	-0.21412268203822293
2214	4	-0.37535726445804585
2214	5	-0.15894451735180581
2214	6	-0.14206627325200571
2214	7	-0.3775853656351842
2214	8	0.011170660738180516
2214	9	0.07696070052125492
2214	10	-1.7661531387461835
2214	11	-0.6573563217082068
2214	12	-0.340557515048836
2214	13	-0.1597977118482916
2214	14	-0.6395828748301184
2214	15	-0.44346998201575616
2214	16	-0.5612400348532205
2214	17	-0.2993224129090706
2214	18	-0.40314497882175715
2214	19	-1.1859934192697494
2214	20	-2.2949600919555024
2214	21	-1.5579310350510411
2214	22	-1.8001015105612694
2214	23	-2.325339376150822
2214	24	-1.3547990214722105
2214	25	-1.769393919419855
2215	1	8.032118548761957E-4
2215	2	-0.10511667747546127
2215	3	-0.6253869805438583
2215	4	-0.004484398400272482
2215	5	0.06462484871490659
2215	6	0.15102306539510124
2215	7	0.06689583290842194
2215	8	0.14236373182956896
2215	9	0.08781637894745131
2215	10	-0.031205921538663972
2215	11	-0.3197092135800901
2215	12	-0.123949012436114
2215	13	-0.053195975003959006
2215	14	0.20552374069182378
2215	15	-0.28003507099410996
2215	16	-0.08690693859766441
2215	17	0.07246879506874393
2215	18	0.003200670837884445
2215	19	-1.9160846572149168
2215	20	-1.9433644894779718
2215	21	-2.8142506009877764
2215	22	-2.0170145862432634
2215	23	-3.0776886966314976
2215	24	-2.1862650374233894
2215	25	-1.2792534448837714
2216	1	-1.1529442152914235
2216	2	-0.9053895412646362
2216	3	-0.6503039230671845
2216	4	-0.6213907354364139
2216	5	-1.4927534553944355
2216	6	-1.2175067682610106
2216	7	-0.8308026533199708
2216	8	-4.857600639914793
2216	9	-3.966231885176679
2216	10	-1.7998248973765598
2216	11	-0.853248351361998
2216	12	-0.8929398385180036
2216	13	-1.6348993919539936
2216	14	-3.1070168472602475
2216	15	-1.8050384227687601
2216	16	-2.8979201904486516
2216	17	-2.4493730569709697
2216	18	-2.3643876897400915
2216	19	-1.2204999710690885
2216	20	-0.6758913269265605
2216	21	-0.4527713558775375
2216	22	-0.4214825412028489
2216	23	-0.7192537929601457
2216	24	-0.2465327930650716
2216	25	-0.023788521187618983
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	8.0
7	102.0
8	535.0
9	1323.0
10	2340.0
11	3798.0
12	5862.0
13	8490.0
14	11956.0
15	16911.0
16	20292.0
17	21923.0
18	22892.0
19	23276.0
20	23356.0
21	24728.0
22	25552.0
23	26129.0
24	27664.0
25	29235.0
26	31041.0
27	34056.0
28	38368.0
29	44547.0
30	53388.0
31	64787.0
32	87543.0
33	126127.0
34	185656.0
35	299463.0
36	531499.0
37	1795750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.27200714948552	20.26035076745189	11.48151957787707	23.986122505185524
2	13.042258019653689	23.15251632581565	37.4525122288588	26.352713425671865
3	20.395501311248868	28.582911928504394	28.183196336697602	22.838390423549132
4	12.73453797037204	19.220267514602703	35.16427831597584	32.880916199049416
5	11.582443476397039	40.173100180642514	35.34363747296703	12.900818869993413
6	31.298644658684456	35.14089167998507	18.214271282843722	15.346192378486762
7	23.87691407392958	34.14632651070767	23.314542085243342	18.662217330119407
8	25.57324286102422	36.71532572121246	21.878238576593773	15.833192841169543
9	25.715464055420252	17.99773169331602	21.258046358169526	35.0287578930942
10	15.430969501991726	32.177244854880534	32.961172826691225	19.430612816436515
11	29.42442747325812	27.854580353266456	20.656106088058575	22.06488608541685
12	21.857377133693088	31.240959104709276	29.60261163615996	17.299052125437676
13	27.574827672305403	23.62865165282992	24.066493298615136	24.730027376249545
14	20.54744246885535	26.55603417286736	26.836061526320453	26.06046183195684
15	28.292662944430635	27.24234253414496	22.448421690923624	22.016572830500785
16	19.27102375580742	30.522511837775657	29.932991724703722	20.2734726817132
17	23.560247065986907	30.770862041819523	27.804203131378856	17.86468776081471
18	23.543776234767012	27.839290446650434	29.169647243661785	19.44728607492077
19	21.80273433907878	31.44721229722492	29.47805010447023	17.272003259226068
20	22.67805983587394	28.653343116210493	28.005148550277365	20.663448497638203
21	19.429002046270764	30.505359433302647	29.92483434187308	20.14080417855351
22	20.95429678697566	30.08651694417981	24.83275026940677	24.12643599943776
23	18.264105900054243	30.821065666009883	28.15744447651678	22.757383957419098
24	20.377170261650548	33.987429515697784	26.056822155931407	19.57857806672026
25	21.58024615496339	28.01289848656454	24.11877333102438	26.28808202744769
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10818.0
1	10818.0
2	12406.0
3	13994.0
4	13994.0
5	13994.0
6	16637.0
7	19280.0
8	19280.0
9	19280.0
10	21179.0
11	23078.0
12	23078.0
13	23078.0
14	29724.5
15	36371.0
16	36371.0
17	36371.0
18	51338.0
19	66305.0
20	66305.0
21	66305.0
22	89549.0
23	112793.0
24	112793.0
25	112793.0
26	142913.0
27	173033.0
28	173033.0
29	173033.0
30	203246.0
31	233459.0
32	233459.0
33	233459.0
34	258564.0
35	283669.0
36	283669.0
37	283669.0
38	301195.0
39	318721.0
40	318721.0
41	318721.0
42	349742.0
43	380763.0
44	380763.0
45	380763.0
46	570473.5
47	760184.0
48	760184.0
49	760184.0
50	654687.5
51	549191.0
52	549191.0
53	549191.0
54	409340.0
55	269489.0
56	269489.0
57	269489.0
58	211160.0
59	152831.0
60	152831.0
61	152831.0
62	124500.0
63	96169.0
64	96169.0
65	96169.0
66	73437.0
67	50705.0
68	50705.0
69	50705.0
70	36827.5
71	22950.0
72	22950.0
73	22950.0
74	16259.5
75	9569.0
76	9569.0
77	9569.0
78	6478.0
79	3387.0
80	3387.0
81	3387.0
82	2307.5
83	1228.0
84	1228.0
85	1228.0
86	832.0
87	436.0
88	436.0
89	436.0
90	284.5
91	133.0
92	133.0
93	133.0
94	81.5
95	30.0
96	30.0
97	30.0
98	20.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03360644842538742
2	0.01582791269122724
3	0.02218137060249451
4	0.0252187693407758
5	0.02363040486295898
6	0.00562894078103504
7	0.0013097040431121131
8	6.130529563503508E-4
9	4.7372273899799836E-4
10	5.573208694094098E-5
11	8.638473475845852E-4
12	0.0011703738257597606
13	0.002368613694989992
14	5.294548259389394E-4
15	0.002034221173344346
16	0.002368613694989992
17	0.0022850155645785806
18	0.0023964797384604625
19	4.1799065205705736E-4
20	3.901246085865869E-4
21	0.084991432584935
22	0.08195403384665373
23	0.0822605603248289
24	8.917133910550557E-4
25	0.23217987419596015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3588597.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.52321367390849
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.36706402880506	56.50039253356749
2	7.285316790912819	9.110028368007109
3	1.2329415515453592	2.3126240422413225
4	0.39976890001021315	0.9997934542208766
5	0.1788305011638366	0.5590528817839349
6	0.10197389337302423	0.3825441314713173
7	0.06690106210053653	0.29280085805062867
8	0.049181994089588316	0.24600130602977877
9	0.044691998588898864	0.25148586395589667
>10	0.22961981061145223	2.6718166361903655
>50	0.021995747496182542	0.9567642630684708
>100	0.01535678058570811	1.8387576760458577
>500	0.002254234297249752	0.9739654076712447
>1k	0.002479657726476283	3.1920256072692386
>5k	7.213549749749187E-4	3.4684691923458413
>10k+	9.016937187186485E-4	16.24347777808067
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	103330	2.879398271803716	No Hit
CCCATGTACTCTGCGTTGATACCAC	99627	2.7762103128325637	No Hit
GAGTACATGGGAAGCAGTGGTATCA	50814	1.4159851329084876	No Hit
CATGTACTCTGCGTTGATACCACTG	41828	1.1655808662828397	No Hit
GCGTTGATACCACTGCTTCCCATGT	33229	0.925960758480264	No Hit
GCTTCCCATGTACTCTGCGTTGATA	28649	0.7983342793855092	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	27249	0.7593218185268505	No Hit
GTATCAACGCAGAGTACATGGGAAG	26357	0.7344653077511908	No Hit
ACTCTGCGTTGATACCACTGCTTCC	22165	0.6176508535229784	No Hit
TATCAACGCAGAGTACATGGGAAGC	20399	0.5684394207541276	No Hit
GGTATCAACGCAGAGTACATGGGAA	18036	0.5025919600334058	No Hit
CAGTGGTATCAACGCAGAGTACATG	14828	0.4131976925801365	No Hit
GTGGTATCAACGCAGAGTACATGGG	13921	0.38792319115241974	No Hit
GCAGTGGTATCAACGCAGAGTACAT	12343	0.3439505745560173	No Hit
GTACATGGTAAGCAGTGGTATCAAC	11151	0.31073425073921646	No Hit
ATACCACTGCTTCCCATGTACTCTG	11133	0.31023266195674803	No Hit
GTACTCTGCGTTGATACCACTGCTT	10546	0.2938752944395818	No Hit
ACATGGGAAGCAGTGGTATCAACGC	10274	0.28629573061561386	No Hit
CATGGGAAGCAGTGGTATCAACGCA	10266	0.2860728022678501	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	10102	0.28150277113869293	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	9457	0.26352917310023943	No Hit
GTTGATACCACTGCTTCCCATGTAC	8664	0.24143140062815638	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	8539	0.23794814519434754	No Hit
TACCACTGCTTCCCATGTACTCTGC	8525	0.23755802058576095	No Hit
CTTCCCATGTACTCTGCGTTGATAC	8449	0.2354402012820052	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8254	0.23000632280526348	No Hit
CCACTGCTTCCCATGTACTCTGCGT	8155	0.2272475845016869	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	8104	0.22582641628469288	No Hit
GATACCACTGCTTCCCATGTACTCT	8104	0.22582641628469288	No Hit
ACCATGTACTCTGCGTTGATACCAC	8055	0.2244609801546398	No Hit
CCATGTACTCTGCGTTGATACCACT	7563	0.21075088676716833	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7270	0.20258613603032047	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	6702	0.18675822333909325	No Hit
TTCCCATGTACTCTGCGTTGATACC	6080	0.1694255443004606	No Hit
TGATACCACTGCTTCCCATGTACTC	5829	0.16243116738937252	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	5296	0.1475785662196117	No Hit
CCCCATGTACTCTGCGTTGATACCA	4906	0.13671080926612825	No Hit
GCTTACCATGTACTCTGCGTTGATA	4368	0.12171887787901511	No Hit
GAGTACATGGTAAGCAGTGGTATCA	3895	0.10853823931748258	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3894	0.1085103732740121	No Hit
ATCAACGCAGAGTACATGGGAAGCA	3854	0.10739573153519329	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.7866043470470493E-5	0.0	0.0	0.0
3	0.0	2.7866043470470493E-5	0.0	2.7866043470470493E-5	0.0
4	0.0	2.7866043470470493E-5	0.0	5.5732086940940986E-5	0.0
5	0.0	2.7866043470470493E-5	0.0	5.5732086940940986E-5	0.0
6	2.7866043470470493E-5	2.7866043470470493E-5	0.0	5.5732086940940986E-5	0.0
7	2.7866043470470493E-5	2.7866043470470493E-5	0.0	5.5732086940940986E-5	0.0
8	2.7866043470470493E-5	2.7866043470470493E-5	0.0	5.5732086940940986E-5	0.0
9	2.7866043470470493E-5	2.7866043470470493E-5	0.0	1.1146417388188197E-4	0.0
10	2.7866043470470493E-5	2.7866043470470493E-5	0.0	1.6719626082282296E-4	0.0
11	2.7866043470470493E-5	2.7866043470470493E-5	0.0	2.2292834776376394E-4	0.0
12	2.7866043470470493E-5	2.7866043470470493E-5	0.0	2.5079439123423445E-4	0.0
13	2.7866043470470493E-5	2.7866043470470493E-5	0.0	3.3439252164564593E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTCT	50	0.001505298	13.29403	9
GTCGTAG	60	4.0920413E-4	12.667512	1
AATACCG	55	0.0030759526	12.087672	5
TATCGAC	115	6.866685E-8	11.591199	19
CCGGTCA	60	0.005897874	11.078359	9
CGCAAAA	310	0.0	11.031457	2
CGCTGCG	95	1.3682498E-5	10.995063	9
AAGCGGT	150	2.1518645E-9	10.761834	10
GATACGA	75	0.002634412	10.140088	18
GTATAGA	455	0.0	10.022647	1
GTACGCT	105	4.1260668E-5	9.948468	6
TACGCTG	105	4.129226E-5	9.947775	7
GTACAAA	785	0.0	9.561149	1
GTCCTAG	290	0.0	9.500634	1
ATGTACG	110	6.856229E-5	9.498118	4
GAAGCGG	180	4.2200554E-9	9.495736	9
GAACAAA	1530	0.0	9.376442	1
TTAGACA	355	0.0	9.364342	4
GCCTTAC	185	6.699338E-9	9.243861	1
GGTATCG	175	2.6442649E-8	9.234724	17
>>END_MODULE