FastQCFastQC Report
Wed 25 May 2016
SRR1294763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588597
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1081153.0127372898099174No Hit
GTACATGGGAAGCAGTGGTATCAAC1027512.8632638326343134No Hit
GAGTACATGGGAAGCAGTGGTATCA568151.583209259774781No Hit
CATGTACTCTGCGTTGATACCACTG469121.3072518312867119No Hit
GCGTTGATACCACTGCTTCCCATGT361021.006019901370926No Hit
ACGCAGAGTACATGGGAAGCAGTGG336060.9364662568686314No Hit
GCTTCCCATGTACTCTGCGTTGATA318440.8873662882736624No Hit
GTATCAACGCAGAGTACATGGGAAG284540.7929004009087673No Hit
ACTCTGCGTTGATACCACTGCTTCC252500.7036175976293799No Hit
TATCAACGCAGAGTACATGGGAAGC241130.671933906203455No Hit
GGTATCAACGCAGAGTACATGGGAA201690.5620302307559194No Hit
CAGTGGTATCAACGCAGAGTACATG179910.5013379880772346No Hit
GTGGTATCAACGCAGAGTACATGGG153120.42668485761984415No Hit
GCAGTGGTATCAACGCAGAGTACAT139390.3884247799348882No Hit
CATGGGAAGCAGTGGTATCAACGCA132890.37031185167908237No Hit
ACATGGGAAGCAGTGGTATCAACGC127550.35543138446585115No Hit
ATACCACTGCTTCCCATGTACTCTG124980.34826981129394025No Hit
GGGAAGCAGTGGTATCAACGCAGAG113960.31756143138948173No Hit
GTACATGGTAAGCAGTGGTATCAAC110150.3069444688272325No Hit
CTGCTTCCCATGTACTCTGCGTTGA109530.30521677413206333No Hit
AAGCAGTGGTATCAACGCAGAGTAC107280.29894691435120746No Hit
GTACTCTGCGTTGATACCACTGCTT105380.29365236609181805No Hit
GCAGAGTACATGGGAAGCAGTGGTA96120.2678484098381624No Hit
CTTCCCATGTACTCTGCGTTGATAC95040.2648388771433516No Hit
GTATCAACGCAGAGTACTTTTTTTT94470.26325051266553473No Hit
GATACCACTGCTTCCCATGTACTCT94420.2631111824481824No Hit
TACCACTGCTTCCCATGTACTCTGC93770.2612998896226018No Hit
GTTGATACCACTGCTTCCCATGTAC93370.260185247883783No Hit
CCACTGCTTCCCATGTACTCTGCGT91150.25399898623333855No Hit
ACCATGTACTCTGCGTTGATACCAC89770.2501534722344136No Hit
CCATGTACTCTGCGTTGATACCACT89180.24850937566965586No Hit
ATGGGAAGCAGTGGTATCAACGCAG83120.23162255332655077No Hit
GTACATGGGTAAGCAGTGGTATCAA79980.222872615676823No Hit
GGTATCAACGCAGAGTACTTTTTTT77830.21688141633067187No Hit
TATCAACGCAGAGTACTTTTTTTTT73450.20467608929060577No Hit
TTCCCATGTACTCTGCGTTGATACC64520.1797917124714756No Hit
GGTAAGCAGTGGTATCAACGCAGAG63700.17750669690689702No Hit
TGATACCACTGCTTCCCATGTACTC61570.17157122964768684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA57570.16042481225949862No Hit
CCCCATGTACTCTGCGTTGATACCA55100.1535418995222924No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54030.15056023287095208No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51550.1436494540902754No Hit
GCTTACCATGTACTCTGCGTTGATA50890.14181029522122435No Hit
ATCAACGCAGAGTACATGGGAAGCA48800.13598629213589603No Hit
GAGTACATGGTAAGCAGTGGTATCA45500.12679049779064075No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37870.10552870662267175No Hit
GGAAGCAGTGGTATCAACGCAGAGT37310.10396820818832542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG307.6457835E-419.0292974
TACACCG604.041474E-412.6861985
CGCACTG752.0457714E-411.4175785
GAACCGA700.001497716310.8523646
AACCGAG909.57958E-510.54929357
CTAGGAC2650.010.0532133
CGCAAAA1054.038693E-59.9678662
ACGCAAA1701.6130798E-89.5191671
CGTACAC1002.7147602E-49.51464753
CGTTTAT800.0045405739.49423114
TGGACTG3700.09.2574955
TTAGACA3500.09.2428014
TTGGACT3050.09.0467154
GTCTAGG2750.08.999941
TATTCCG850.0073452978.9549635
GTATTAG4150.08.9457241
GTCTTAG3300.08.9422481
TGTGCGG850.0074775778.93549810
TAGACAG4900.08.9321185
GTCCTAG3200.08.9242191
Produced by FastQC (version 0.11.5)