FastQCFastQC Report
Wed 25 May 2016
SRR1294762_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294762_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3231079
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC559461.7314958872871882No Hit
CCCATGTACTCTGCGTTGATACCAC535201.6564126101528311No Hit
GAGTACATGGGAAGCAGTGGTATCA279240.8642314223824301No Hit
CATGTACTCTGCGTTGATACCACTG223380.6913479986097524No Hit
GCGTTGATACCACTGCTTCCCATGT177320.5487950000603514No Hit
GCTTCCCATGTACTCTGCGTTGATA165610.5125532368598849No Hit
GTATCAACGCAGAGTACATGGGAAG151760.46968829917188654No Hit
ACGCAGAGTACATGGGAAGCAGTGG150520.46585057189873724No Hit
ACTCTGCGTTGATACCACTGCTTCC119030.36839086880884064No Hit
TATCAACGCAGAGTACATGGGAAGC108770.3366367705648794No Hit
GGTATCAACGCAGAGTACATGGGAA99660.30844185487262926No Hit
GTGGTATCAACGCAGAGTACATGGG83280.25774671557086654No Hit
CAGTGGTATCAACGCAGAGTACATG82950.256725384925593No Hit
GCAGTGGTATCAACGCAGAGTACAT66940.20717537392307647No Hit
ATACCACTGCTTCCCATGTACTCTG61930.19166971776301353No Hit
GTACATGGTAAGCAGTGGTATCAAC61650.19080313418520561No Hit
CATGGGAAGCAGTGGTATCAACGCA58010.1795375476737028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA57050.1765664039783614No Hit
GTACTCTGCGTTGATACCACTGCTT55710.17241918257028072No Hit
ACATGGGAAGCAGTGGTATCAACGC55140.17065506600117175No Hit
CTGCTTCCCATGTACTCTGCGTTGA54270.1679624670272686No Hit
GGGAAGCAGTGGTATCAACGCAGAG52610.16282486438740743No Hit
TACCACTGCTTCCCATGTACTCTGC46610.14425521629152366No Hit
AAGCAGTGGTATCAACGCAGAGTAC45570.14103647728823715No Hit
GCAGAGTACATGGGAAGCAGTGGTA45350.14035559019138807No Hit
CTTCCCATGTACTCTGCGTTGATAC44640.13815818183337517No Hit
GTTGATACCACTGCTTCCCATGTAC44410.13744634532303296No Hit
GATACCACTGCTTCCCATGTACTCT43380.13425855573323958No Hit
CCACTGCTTCCCATGTACTCTGCGT43330.13410380866577387No Hit
ACCATGTACTCTGCGTTGATACCAC42270.1308231708355011No Hit
CCATGTACTCTGCGTTGATACCACT41680.12899715543940585No Hit
GTACATGGGTAAGCAGTGGTATCAA40850.12642835411947526No Hit
ATGGGAAGCAGTGGTATCAACGCAG37290.11541036291591755No Hit
TTCCCATGTACTCTGCGTTGATACC32810.10154502567099102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG853.936688E-612.2970041
TAATACG600.005887365411.0809594
TTAGACA2600.010.9591894
GGTTTAG1658.0035534E-1110.9419621
TAGACAG3650.010.4088895
GTACAAA7750.010.2991911
CTAGACT1856.002665E-1110.268074
CGCAAAA1554.0699888E-89.8061712
TACGACC900.00111638429.4979644
TCTAGAC2750.09.3262843
AGCACCG1451.7123093E-69.170595
GTCCTAG2800.09.1628651
TAATACT4150.09.1546654
GTGCTAG2600.09.136761
GTTAGAC1252.7593256E-59.1190343
TTATACT4700.09.0937964
ACAGTCC3350.09.0700158
ACGCAAA2106.657501E-109.0497431
TCCGTAC1054.4995497E-49.046663
ACTGTCG1054.5176857E-49.0430198
Produced by FastQC (version 0.11.5)