##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294762_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3231079
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.056210015292105	33.0	33.0	33.0	27.0	33.0
2	31.070696197771703	33.0	33.0	33.0	27.0	33.0
3	30.9837382496683	33.0	33.0	33.0	27.0	33.0
4	34.29521098060431	37.0	37.0	37.0	27.0	37.0
5	34.707384437211225	37.0	37.0	37.0	27.0	37.0
6	34.87356390852715	37.0	37.0	37.0	33.0	37.0
7	34.88054981014082	37.0	37.0	37.0	33.0	37.0
8	34.79354512842304	37.0	37.0	37.0	27.0	37.0
9	34.46816682600456	37.0	37.0	37.0	27.0	37.0
10	34.573789436903276	37.0	37.0	37.0	27.0	37.0
11	34.84630583158134	37.0	37.0	37.0	33.0	37.0
12	34.767337784065326	37.0	37.0	37.0	27.0	37.0
13	34.66640122386361	37.0	37.0	37.0	27.0	37.0
14	35.86883081472165	40.0	37.0	40.0	27.0	40.0
15	36.14787351222301	40.0	37.0	40.0	27.0	40.0
16	36.15289598304467	40.0	37.0	40.0	27.0	40.0
17	36.12813552376776	40.0	37.0	40.0	27.0	40.0
18	36.11646821386911	40.0	37.0	40.0	27.0	40.0
19	36.15550068568425	40.0	37.0	40.0	27.0	40.0
20	36.05992951580571	40.0	37.0	40.0	27.0	40.0
21	35.98167639974139	40.0	37.0	40.0	27.0	40.0
22	35.87830288272122	40.0	37.0	40.0	27.0	40.0
23	35.506814596609985	37.0	37.0	40.0	27.0	40.0
24	35.83861706878724	37.0	37.0	40.0	27.0	40.0
25	34.76434404729813	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.42984224618639644
1101	2	0.5885532060018086
1101	3	0.32643346303748544
1101	4	0.47391623883972045
1101	5	0.40491847264623004
1101	6	0.3467771241438413
1101	7	0.3983695437206194
1101	8	0.37942470091260816
1101	9	0.6515699887655586
1101	10	0.6406474831215903
1101	11	0.4058394422146918
1101	12	0.4252953054092927
1101	13	0.45346675234782907
1101	14	1.0535531681254042
1101	15	0.8548921470193704
1101	16	0.8748852943495535
1101	17	0.8428191371439624
1101	18	0.7837117128675573
1101	19	0.7989404812001482
1101	20	0.9365336142840803
1101	21	0.9549647795704388
1101	22	0.9282948690923192
1101	23	1.2841220497421233
1101	24	1.0769075278718816
1101	25	0.9247156151059812
1102	1	0.2113405944353275
1102	2	0.4567321884683935
1102	3	0.21784212278311088
1102	4	0.13558352492153602
1102	5	0.16091840247757716
1102	6	0.10897605563117452
1102	7	0.2940980631942125
1102	8	0.24647550016213415
1102	9	0.44896956022002854
1102	10	0.4629069745085488
1102	11	0.25884844225108594
1102	12	0.27243602116492127
1102	13	0.26476311686364795
1102	14	0.9216675120260547
1102	15	0.6986747850189658
1102	16	0.4909405078329172
1102	17	0.6486020042342417
1102	18	0.6551674082754033
1102	19	0.5846840329932519
1102	20	0.7332485880798529
1102	21	0.7076462941599146
1102	22	0.7268605637916821
1102	23	1.1117446383009266
1102	24	0.7117993079087483
1102	25	0.6804770259093189
1103	1	0.3050589488293234
1103	2	0.5659803158444952
1103	3	0.2566813692513179
1103	4	0.07957054802308505
1103	5	0.18949914397773426
1103	6	0.1570731757693693
1103	7	0.2939800375209529
1103	8	0.30813858581755227
1103	9	0.579988095689906
1103	10	0.7398425645953424
1103	11	0.41857198004610296
1103	12	0.4138818971294427
1103	13	0.35336099662904985
1103	14	1.0583013101567857
1103	15	0.8145657369028783
1103	16	0.5460767604908199
1103	17	0.6391021536125621
1103	18	0.7122292172901439
1103	19	0.6918290127409463
1103	20	0.7949799066366694
1103	21	0.798387562603807
1103	22	0.7518449936007627
1103	23	1.2245853628096341
1103	24	0.8341832637755502
1103	25	0.8151787672058859
1104	1	0.21409343485009202
1104	2	0.5171481326944196
1104	3	0.23439226458568285
1104	4	0.2508259485282309
1104	5	0.2027118176268914
1104	6	0.13965052533256994
1104	7	0.14720837620001248
1104	8	0.341462598040728
1104	9	0.5435018620653196
1104	10	0.5711449045417964
1104	11	0.33497814255200353
1104	12	0.3133015536813133
1104	13	0.3686340529862946
1104	14	0.8067549797178657
1104	15	0.702385654445834
1104	16	0.5815745390754969
1104	17	0.5854965534339271
1104	18	0.49602171963955044
1104	19	0.6328644698266217
1104	20	0.6737505217673387
1104	21	0.6641440993801098
1104	22	0.7244031931518222
1104	23	1.0696476438244105
1104	24	0.812052061498413
1104	25	0.5683432493243075
1105	1	0.2140330219844735
1105	2	0.27468506432497364
1105	3	0.240656131599561
1105	4	0.08638157367425237
1105	5	0.1936765530671778
1105	6	0.07570973608455489
1105	7	0.09730440535109608
1105	8	0.2811102306158233
1105	9	-1.6840576595398744
1105	10	-0.19371892161660043
1105	11	-0.0712980800874945
1105	12	0.17220705004041292
1105	13	0.14315950054067628
1105	14	-1.584402112675761
1105	15	-0.17406017987376288
1105	16	-0.03359695752013181
1105	17	0.22913803959725243
1105	18	0.10438008705182256
1105	19	0.2931538815845016
1105	20	0.43428710576792895
1105	21	0.5371863769519649
1105	22	0.5840651302647615
1105	23	0.9118010706588819
1105	24	0.6678842666413942
1105	25	0.3384980649720646
1106	1	0.048979444218606005
1106	2	0.1172251474442838
1106	3	0.07650771453665683
1106	4	-1.07755464692341
1106	5	-0.30676768588762116
1106	6	-0.08657761788142437
1106	7	-0.016124593330317794
1106	8	0.1313793869041291
1106	9	0.4251550122766474
1106	10	0.38289973182632053
1106	11	0.27536265299344365
1106	12	0.05438007704010772
1106	13	0.18555494167489428
1106	14	0.6527893802262525
1106	15	0.47242726881581376
1106	16	0.2662826663760427
1106	17	0.22375114395310192
1106	18	0.25145130351344847
1106	19	0.25561612322328386
1106	20	0.3886689389425939
1106	21	0.5002443893076318
1106	22	0.5948175589111528
1106	23	0.8312794235428598
1106	24	0.36399908239185663
1106	25	0.2854783710428208
1107	1	0.3504291572962259
1107	2	0.3260442353331392
1107	3	0.2826085904049762
1107	4	0.45679406084722984
1107	5	0.42754650490497426
1107	6	0.28665446564966857
1107	7	0.25788412220664014
1107	8	0.352887778844412
1107	9	0.47602872328324963
1107	10	0.48772347950720984
1107	11	0.25731890272378166
1107	12	0.2908084327375633
1107	13	0.29985791444963183
1107	14	0.8251499100618602
1107	15	0.5874378396403444
1107	16	0.5248234611294578
1107	17	0.6125719576908608
1107	18	0.5525429029439195
1107	19	0.5368173140590287
1107	20	0.7399482244411502
1107	21	0.6934963201010902
1107	22	0.5402979920992266
1107	23	0.8660207619386853
1107	24	0.7371362442333407
1107	25	0.5086996680182736
1108	1	0.3588472246865173
1108	2	0.3257187415204612
1108	3	0.4045926255217047
1108	4	0.5977774200460857
1108	5	0.6030681096735009
1108	6	0.4073432949653224
1108	7	0.3598876832172593
1108	8	0.3417324728237432
1108	9	0.13151327068391794
1108	10	0.38591833339960857
1108	11	0.25006279969144884
1108	12	0.2598826456069716
1108	13	0.29875089934834165
1108	14	-1.0899308924232471
1108	15	-0.05015944085231894
1108	16	0.21940872309455273
1108	17	0.4059993543164637
1108	18	0.5865413666056583
1108	19	0.42210184709912113
1108	20	0.618265126374979
1108	21	0.6244849557357952
1108	22	0.7825973696553348
1108	23	1.008932247136329
1108	24	0.896807486145363
1108	25	0.7277014010907479
1109	1	0.4293799844775883
1109	2	0.33929162977365834
1109	3	0.45722434439383264
1109	4	0.6340172798153532
1109	5	0.5863439292664552
1109	6	0.4279543053461552
1109	7	0.32679734322154985
1109	8	0.44170890016589937
1109	9	0.6368942172413341
1109	10	0.52014157887605
1109	11	0.39014996535406254
1109	12	0.4012769417327249
1109	13	0.48095868257914276
1109	14	0.7262130106771778
1109	15	0.5428710281372275
1109	16	0.48478810995732147
1109	17	0.5094780051096919
1109	18	0.528228282717123
1109	19	0.44671932312324714
1109	20	0.6765073150519498
1109	21	0.6494453873458426
1109	22	0.6963701824051327
1109	23	1.1619382927254946
1109	24	0.8806088164940107
1109	25	0.8042637986250156
1110	1	0.530279117306069
1110	2	0.3653441768859871
1110	3	0.4916539279629113
1110	4	0.7326079447556566
1110	5	0.6174454565183467
1110	6	0.48957978952598324
1110	7	0.45437668887154814
1110	8	0.4600852928629422
1110	9	0.5248807091680447
1110	10	0.5349364915423394
1110	11	0.3083340008388262
1110	12	0.33577575213733013
1110	13	0.2760946246806668
1110	14	0.7280819538957601
1110	15	0.5680669668295479
1110	16	0.5435980521458816
1110	17	0.5295531073858797
1110	18	0.6838524304155484
1110	19	0.5789672866665043
1110	20	0.6113277786314768
1110	21	0.7381226062313928
1110	22	0.8550370013980384
1110	23	0.926564558484003
1110	24	0.7413731133996109
1110	25	0.7664719306701002
1111	1	0.1793068862722187
1111	2	0.0394427913455182
1111	3	0.28699379541252057
1111	4	0.6636648085443255
1111	5	0.3634468129217012
1111	6	0.29652097366787444
1111	7	0.19233802168323422
1111	8	0.29602003922902753
1111	9	0.3094749424858705
1111	10	0.23433035430181093
1111	11	0.10804054423667964
1111	12	0.08178261580851398
1111	13	0.17439308452548374
1111	14	-1.6789193108666538
1111	15	-0.5435365674559165
1111	16	-0.07573996251196746
1111	17	0.06074605209115447
1111	18	0.17380680674023097
1111	19	0.25644883217915293
1111	20	0.24130818450683478
1111	21	0.3220123204761549
1111	22	0.1942820563121046
1111	23	-1.9006227373799334
1111	24	-0.3958596299070791
1111	25	0.03722639992809462
1112	1	-0.13785616496041442
1112	2	-0.3544320924780777
1112	3	0.03607536344386375
1112	4	0.21468944259133593
1112	5	-0.8211765022969146
1112	6	-1.0109669452019787
1112	7	-1.1434803458689444
1112	8	-0.8945661278644366
1112	9	-0.752728089737964
1112	10	-1.0301708285266926
1112	11	-0.9626789676915237
1112	12	-0.9443290191126863
1112	13	-1.0124727911474167
1112	14	-0.7205278088639915
1112	15	-1.033818376330224
1112	16	-1.0500838778581496
1112	17	-1.0360009084394122
1112	18	-1.2018876231009514
1112	19	-1.0128152077313146
1112	20	-0.9797535730603144
1112	21	-1.8810044537015216
1112	22	-1.788452810285122
1112	23	-1.3400908429245462
1112	24	-1.7375469354542048
1112	25	-2.042581944230726
1113	1	0.14085816097504278
1113	2	-0.05050515660773769
1113	3	0.07606334581451435
1113	4	0.456416054118705
1113	5	0.201393524062766
1113	6	0.09647642090600073
1113	7	0.20058160860487817
1113	8	0.2718967542254518
1113	9	0.3971909518858112
1113	10	0.1583545997198712
1113	11	0.07817177347934745
1113	12	0.11521747104823277
1113	13	0.10641399880750413
1113	14	0.29548424584282884
1113	15	0.13330797314999643
1113	16	0.16236706259705613
1113	17	0.1276560793797188
1113	18	0.2824536549297747
1113	19	0.19318301438976704
1113	20	-0.45002879757537073
1113	21	-0.48364062655358
1113	22	0.11429669651020902
1113	23	-0.07869767532609018
1113	24	0.24654123930182692
1113	25	-0.9278576665320699
1114	1	-0.9158897472655738
1114	2	-0.7026915039969168
1114	3	-0.4945682474953763
1114	4	-1.5201920458915552
1114	5	-0.7249425438557608
1114	6	-0.5354739890000175
1114	7	-0.38555620488078546
1114	8	-0.38222087062377597
1114	9	-0.39974167205156164
1114	10	-0.3366906595987018
1114	11	-0.3778916819146332
1114	12	-0.5498630432236808
1114	13	-0.5746056652557385
1114	14	-0.45069072661303267
1114	15	-1.3610739796725113
1114	16	-0.8154963642401967
1114	17	-0.8445642233286677
1114	18	-0.7437043877662717
1114	19	-0.7078183942164031
1114	20	-1.8965110260823295
1114	21	-6.813113130768233
1114	22	-7.6216511766790305
1114	23	-7.7547801384856925
1114	24	-5.66763595880391
1114	25	-7.083754874417814
1115	1	-0.9206951995488915
1115	2	-0.9042947915006891
1115	3	-0.6436943691170498
1115	4	-0.5355676092873551
1115	5	-0.44095009797723606
1115	6	-0.6880439501214397
1115	7	-0.9972240080292849
1115	8	-0.7017377030782086
1115	9	-2.8187378738419397
1115	10	-1.5449141612923043
1115	11	-1.024257874189459
1115	12	-0.976375291278579
1115	13	-1.2415665756942573
1115	14	-0.9702329088559196
1115	15	-1.9093113717798218
1115	16	-1.263711553628454
1115	17	-2.9057791263769204
1115	18	-2.67234563549556
1115	19	-1.9518490856499255
1115	20	-1.4994737898271353
1115	21	-1.1378669104815984
1115	22	-0.6753932334866661
1115	23	-0.12910358172031522
1115	24	-1.076374410556916
1115	25	-0.8560317047905528
1116	1	-1.4465330173858142
1116	2	-0.808562008040699
1116	3	-0.5640393287065812
1116	4	-0.16072305790591912
1116	5	-0.1747740341206665
1116	6	-0.23651945580309786
1116	7	-0.6603590057703599
1116	8	-1.5874027426231052
1116	9	-0.8131094050737673
1116	10	-2.401378800177582
1116	11	-1.1407578749001672
1116	12	-0.7272041448463753
1116	13	-0.7522086013998859
1116	14	-1.0143597434077165
1116	15	-1.141810922724737
1116	16	-0.7670703958840264
1116	17	-0.4104432395329667
1116	18	-1.0558798235684321
1116	19	-0.756192213259304
1116	20	-0.9482685304700524
1116	21	-0.4190304168802541
1116	22	-0.07530762686729986
1116	23	0.09334873109852282
1116	24	-0.017452174179553026
1116	25	0.04694015335187629
1201	1	0.09637129526107202
1201	2	0.2951823260927853
1201	3	0.060950316727637954
1201	4	0.1438409883981393
1201	5	0.1242891414146925
1201	6	0.07721658072116355
1201	7	0.11201040704345644
1201	8	0.1442099909518859
1201	9	0.46252026951496816
1201	10	0.3100380352750065
1201	11	0.32342604123478225
1201	12	0.324733289850073
1201	13	0.3041448657520718
1201	14	0.9470750919540691
1201	15	0.6196995574093975
1201	16	0.6461384752265289
1201	17	0.5232579432560556
1201	18	0.334820171349719
1201	19	0.227495805171948
1201	20	0.5960476564685919
1201	21	0.5014214370775534
1201	22	0.6694817791209218
1201	23	0.34253015598036285
1201	24	0.5138319143029904
1201	25	0.4124270576687934
1202	1	-0.32036073244934826
1202	2	-0.060243261967961814
1202	3	-0.14822712840792462
1202	4	-0.17848496812577963
1202	5	-0.10036624275954864
1202	6	-0.09999480865710098
1202	7	-0.08432657807464494
1202	8	0.10796046756594535
1202	9	0.07756458408069022
1202	10	0.2926940328887184
1202	11	0.07625707328233489
1202	12	0.17983293825426472
1202	13	0.23422195405406399
1202	14	0.43112251411006497
1202	15	0.36129576953162257
1202	16	0.36073132142144715
1202	17	0.13849134987365375
1202	18	0.20979073113765168
1202	19	0.20169250795697735
1202	20	0.32960972016419987
1202	21	0.37040408188943985
1202	22	0.2915062536691764
1202	23	0.6593570404313596
1202	24	0.28444177168053386
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2211	4	0.34078595293798486
2211	5	0.19446747767504746
2211	6	0.14605157864819063
2211	7	0.12266726230994607
2211	8	0.22205688464521955
2211	9	0.21614130090841144
2211	10	0.3491915216730632
2211	11	0.03168277791339591
2211	12	0.027345960910167832
2211	13	-0.058781991294672764
2211	14	0.2533894170706148
2211	15	0.07046749935629038
2211	16	0.059198020827629705
2211	17	0.07964404965784411
2211	18	0.18264532419892987
2211	19	0.18177913323266637
2211	20	0.12973110942674992
2211	21	0.2069895403256794
2211	22	0.31089310740974696
2211	23	0.37230211791883505
2211	24	0.42271584570188736
2211	25	0.47384432333814175
2212	1	-0.2898243106909817
2212	2	-0.23994177613557355
2212	3	-0.12740611534703916
2212	4	-0.4154860105359077
2212	5	-0.09988543485082602
2212	6	-0.3825759761822596
2212	7	-0.3274895420988031
2212	8	-0.09739282256523296
2212	9	-0.09737683143740838
2212	10	-0.4737450932420444
2212	11	-0.38244520139165417
2212	12	-0.45251171875924
2212	13	-0.3070817512557795
2212	14	-0.32277077802591236
2212	15	-0.46360755047796687
2212	16	-0.21011599926615787
2212	17	-0.28487064005846463
2212	18	-0.2870675402340126
2212	19	-0.40683430366622275
2212	20	-0.5092321677726233
2212	21	-0.30535981802369605
2212	22	-0.4641485524094122
2212	23	0.04800948334079891
2212	24	-0.12090158049190336
2212	25	-0.6521003135934507
2213	1	-0.06137763115809847
2213	2	-1.2575229839029731
2213	3	-0.18366846328023811
2213	4	0.22732838198520966
2213	5	0.12345802979101705
2213	6	0.10459560756910946
2213	7	0.13407302160553058
2213	8	0.1886732558259041
2213	9	0.25799571434423996
2213	10	-0.29810007611824574
2213	11	0.02444595762213453
2213	12	0.03386878970107432
2213	13	-0.27030986373873844
2213	14	0.13281662566143382
2213	15	0.01915007994756479
2213	16	0.04498908359353493
2213	17	0.19561595558715794
2213	18	0.19825886785396563
2213	19	0.17793739697847855
2213	20	0.28331572411342876
2213	21	-0.40008428208084723
2213	22	-0.699788949046706
2213	23	-0.0035375833936797108
2213	24	0.193726153733067
2213	25	-0.489903387733996
2214	1	-0.1071733900338998
2214	2	-0.36441311454651526
2214	3	-0.2951611147712576
2214	4	-0.41962015142801334
2214	5	-0.10224698149780664
2214	6	-0.08061844922223571
2214	7	-0.3636217945990481
2214	8	-0.013092423776818407
2214	9	0.07512979550378418
2214	10	-1.9000310987795714
2214	11	-0.8486656069014629
2214	12	-0.492189839135996
2214	13	-0.1812137456647278
2214	14	-0.7172773965880097
2214	15	-0.5257829430245167
2214	16	-0.5974211234502249
2214	17	-0.45026613099448554
2214	18	-0.3742370249508582
2214	19	-1.1872681000981657
2214	20	-2.370650165416734
2214	21	-1.7475906039516929
2214	22	-1.9162271931809443
2214	23	-2.462698531126051
2214	24	-1.5088548769136736
2214	25	-1.9522342168513376
2215	1	-0.12150266611531535
2215	2	-0.3008620834271163
2215	3	-0.6956138827936833
2215	4	-0.2228926798081332
2215	5	-0.1437044659456106
2215	6	-0.1464053977391515
2215	7	-0.21201546727748877
2215	8	-0.1621316861433968
2215	9	-0.14451871295156593
2215	10	-0.2449553999904026
2215	11	-0.5840016886195087
2215	12	-0.4351228686224502
2215	13	-0.2853048055276588
2215	14	-0.1695561001229393
2215	15	-0.6011945861561117
2215	16	-0.3813105021247196
2215	17	-0.1490951742259412
2215	18	-0.1878093165835395
2215	19	-2.1048667232294562
2215	20	-2.3407584001936783
2215	21	-3.182117174841956
2215	22	-2.2855947151181795
2215	23	-3.506340838879538
2215	24	-2.4246890668075523
2215	25	-1.5854670192307552
2216	1	-1.3407453679574388
2216	2	-1.1174105850439737
2216	3	-0.7388348232748321
2216	4	-0.6739450597554892
2216	5	-1.867185838625467
2216	6	-1.50250446108965
2216	7	-1.0140902986675258
2216	8	-4.998791023084365
2216	9	-3.9964790692377186
2216	10	-1.9483392396962955
2216	11	-0.9678062663255247
2216	12	-1.1039030873359081
2216	13	-1.8032595189069482
2216	14	-3.235761504529009
2216	15	-1.868706630845729
2216	16	-2.892185975793595
2216	17	-2.674056552831587
2216	18	-2.5368853453277467
2216	19	-1.6034709393683215
2216	20	-0.8450459295292774
2216	21	-0.6755774827332317
2216	22	-0.6530037718003854
2216	23	-0.910486671438683
2216	24	-0.4758011896422403
2216	25	-0.3523388392825524
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	137.0
8	572.0
9	1306.0
10	2204.0
11	3476.0
12	5576.0
13	7619.0
14	10699.0
15	14531.0
16	17205.0
17	18657.0
18	18944.0
19	19353.0
20	19939.0
21	21001.0
22	21781.0
23	22782.0
24	24328.0
25	26141.0
26	27689.0
27	31351.0
28	35611.0
29	41685.0
30	50124.0
31	61378.0
32	83183.0
33	119080.0
34	173870.0
35	280043.0
36	490339.0
37	1580470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34513858445962	19.63766931427255	11.806582359366061	24.210609741901767
2	14.50402662625182	21.78201118342199	37.96714188780918	25.746820302517015
3	20.322280462571186	26.71445801184537	28.19293116535595	24.770330360227497
4	13.397734424750624	17.94600474959081	35.19443076268671	33.461830062971856
5	12.744295539710176	39.22071858986584	34.62814200409646	13.406843866327522
6	32.402315740142576	35.53598773226042	17.3318966491229	14.729799878474099
7	25.92427636227678	33.43848355877389	22.075109573612327	18.562130505337006
8	25.793175851068735	36.60894710469033	20.715377161079022	16.882499883161913
9	25.6792986239773	17.775509408196957	20.655289878442765	35.88990208938298
10	15.776055987550619	31.142022727300866	32.465140866430374	20.616780418718143
11	31.52536969749491	26.49011767181881	21.230973500628284	20.753539130057998
12	22.158266656638467	29.165037858001313	29.344400256400014	19.332295228960202
13	27.701468035743908	23.243321539196728	24.61893961715688	24.436270807902485
14	21.081330185120017	24.81752773643401	26.472072915244908	27.629069163201063
15	26.367380213407877	28.335720626085582	22.51042712525797	22.786472035248572
16	20.974106183126416	30.11566377375694	27.669766942339574	21.240463100777067
17	23.17972271785404	30.07463621999966	27.05130447028092	19.69433659186538
18	23.225410089755492	28.169359331476322	28.115258433921387	20.489972144846796
19	22.505283082869195	30.016298016631033	28.43409671353249	19.044322186967282
20	22.99246657528303	28.300651147128796	27.53081257677417	21.176069700814004
21	21.072127944413367	29.21330738860898	28.697634309481906	21.016930357495752
22	22.10503753730035	28.75941375031671	25.57721458921451	23.558334123168436
23	20.337271596433755	29.354070749971505	27.617348759298448	22.69130889429629
24	21.5195726453402	31.23644399326533	26.36618550064376	20.877797860750718
25	22.309105366633666	27.719577033107463	25.130622037328383	24.840695562930488
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7811.0
1	7811.0
2	8964.0
3	10117.0
4	10117.0
5	10117.0
6	12108.0
7	14099.0
8	14099.0
9	14099.0
10	15555.5
11	17012.0
12	17012.0
13	17012.0
14	22662.0
15	28312.0
16	28312.0
17	28312.0
18	40595.5
19	52879.0
20	52879.0
21	52879.0
22	72681.0
23	92483.0
24	92483.0
25	92483.0
26	119661.5
27	146840.0
28	146840.0
29	146840.0
30	176110.5
31	205381.0
32	205381.0
33	205381.0
34	231603.5
35	257826.0
36	257826.0
37	257826.0
38	279153.5
39	300481.0
40	300481.0
41	300481.0
42	325413.0
43	350345.0
44	350345.0
45	350345.0
46	456952.5
47	563560.0
48	563560.0
49	563560.0
50	503435.0
51	443310.0
52	443310.0
53	443310.0
54	361123.5
55	278937.0
56	278937.0
57	278937.0
58	234343.0
59	189749.0
60	189749.0
61	189749.0
62	159553.0
63	129357.0
64	129357.0
65	129357.0
66	102804.0
67	76251.0
68	76251.0
69	76251.0
70	57247.5
71	38244.0
72	38244.0
73	38244.0
74	27882.0
75	17520.0
76	17520.0
77	17520.0
78	12152.0
79	6784.0
80	6784.0
81	6784.0
82	4670.5
83	2557.0
84	2557.0
85	2557.0
86	1720.0
87	883.0
88	883.0
89	883.0
90	575.5
91	268.0
92	268.0
93	268.0
94	161.5
95	55.0
96	55.0
97	55.0
98	36.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030670868771701342
2	0.01804350806650039
3	0.024357188419100863
4	0.028040168624784475
5	0.030175678155811108
6	0.015227111438624683
7	0.0045186143699983815
8	0.003466334311231635
9	0.005354248534313151
10	0.00232120601198547
11	0.0018260153960952363
12	0.005880388563696524
13	0.002940194281848262
14	0.001237976539725584
15	0.0025688013199305867
16	0.0023521554254786092
17	0.00229025659849233
18	0.002445003665958028
19	3.4044354842453555E-4
20	3.713929619176752E-4
21	0.0828515799211347
22	0.07913765030195795
23	0.07994233505277959
24	0.0012070271262324443
25	0.24289099709415957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3231079.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.22785940710378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.53072381355742	59.517310537469726
2	7.9197161550222885	10.648511284280138
3	1.708699480308927	3.4461662529359947
4	0.6355858943264486	1.7091631657966722
5	0.3208272930108843	1.078426607424871
6	0.1869670848604396	0.7541638136852203
7	0.14734332519368723	0.6933903445487464
8	0.08611779266199884	0.4631611886024777
9	0.07091443868473445	0.4290683322447907
>10	0.3535451868396175	4.3113701341886435
>50	0.023605917643187254	1.087582038119315
>100	0.011071466897202115	1.469452638269244
>500	0.0017203770169044415	0.8077565850048074
>1k	0.002138847101560331	3.3957285353567097
>5k	5.579601134505211E-4	2.466938186465913
>10k+	4.6496676120876766E-4	7.7218103556067925
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	55946	1.7314958872871882	No Hit
CCCATGTACTCTGCGTTGATACCAC	53520	1.6564126101528311	No Hit
GAGTACATGGGAAGCAGTGGTATCA	27924	0.8642314223824301	No Hit
CATGTACTCTGCGTTGATACCACTG	22338	0.6913479986097524	No Hit
GCGTTGATACCACTGCTTCCCATGT	17732	0.5487950000603514	No Hit
GCTTCCCATGTACTCTGCGTTGATA	16561	0.5125532368598849	No Hit
GTATCAACGCAGAGTACATGGGAAG	15176	0.46968829917188654	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	15052	0.46585057189873724	No Hit
ACTCTGCGTTGATACCACTGCTTCC	11903	0.36839086880884064	No Hit
TATCAACGCAGAGTACATGGGAAGC	10877	0.3366367705648794	No Hit
GGTATCAACGCAGAGTACATGGGAA	9966	0.30844185487262926	No Hit
GTGGTATCAACGCAGAGTACATGGG	8328	0.25774671557086654	No Hit
CAGTGGTATCAACGCAGAGTACATG	8295	0.256725384925593	No Hit
GCAGTGGTATCAACGCAGAGTACAT	6694	0.20717537392307647	No Hit
ATACCACTGCTTCCCATGTACTCTG	6193	0.19166971776301353	No Hit
GTACATGGTAAGCAGTGGTATCAAC	6165	0.19080313418520561	No Hit
CATGGGAAGCAGTGGTATCAACGCA	5801	0.1795375476737028	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	5705	0.1765664039783614	No Hit
GTACTCTGCGTTGATACCACTGCTT	5571	0.17241918257028072	No Hit
ACATGGGAAGCAGTGGTATCAACGC	5514	0.17065506600117175	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	5427	0.1679624670272686	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	5261	0.16282486438740743	No Hit
TACCACTGCTTCCCATGTACTCTGC	4661	0.14425521629152366	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4557	0.14103647728823715	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	4535	0.14035559019138807	No Hit
CTTCCCATGTACTCTGCGTTGATAC	4464	0.13815818183337517	No Hit
GTTGATACCACTGCTTCCCATGTAC	4441	0.13744634532303296	No Hit
GATACCACTGCTTCCCATGTACTCT	4338	0.13425855573323958	No Hit
CCACTGCTTCCCATGTACTCTGCGT	4333	0.13410380866577387	No Hit
ACCATGTACTCTGCGTTGATACCAC	4227	0.1308231708355011	No Hit
CCATGTACTCTGCGTTGATACCACT	4168	0.12899715543940585	No Hit
GTACATGGGTAAGCAGTGGTATCAA	4085	0.12642835411947526	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	3729	0.11541036291591755	No Hit
TTCCCATGTACTCTGCGTTGATACC	3281	0.10154502567099102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.09494134931396E-5	0.0	0.0	0.0
6	0.0	3.09494134931396E-5	0.0	0.0	0.0
7	0.0	3.09494134931396E-5	0.0	0.0	0.0
8	0.0	3.09494134931396E-5	0.0	0.0	0.0
9	0.0	3.09494134931396E-5	0.0	0.0	0.0
10	0.0	3.09494134931396E-5	0.0	0.0	0.0
11	0.0	3.09494134931396E-5	0.0	3.09494134931396E-5	0.0
12	0.0	3.09494134931396E-5	0.0	9.28482404794188E-5	0.0
13	0.0	3.09494134931396E-5	0.0	9.28482404794188E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTACG	85	3.936688E-6	12.297004	1
TAATACG	60	0.0058873654	11.080959	4
TTAGACA	260	0.0	10.959189	4
GGTTTAG	165	8.0035534E-11	10.941962	1
TAGACAG	365	0.0	10.408889	5
GTACAAA	775	0.0	10.299191	1
CTAGACT	185	6.002665E-11	10.26807	4
CGCAAAA	155	4.0699888E-8	9.806171	2
TACGACC	90	0.0011163842	9.497964	4
TCTAGAC	275	0.0	9.326284	3
AGCACCG	145	1.7123093E-6	9.17059	5
GTCCTAG	280	0.0	9.162865	1
TAATACT	415	0.0	9.154665	4
GTGCTAG	260	0.0	9.13676	1
GTTAGAC	125	2.7593256E-5	9.119034	3
TTATACT	470	0.0	9.093796	4
ACAGTCC	335	0.0	9.070015	8
ACGCAAA	210	6.657501E-10	9.049743	1
TCCGTAC	105	4.4995497E-4	9.04666	3
ACTGTCG	105	4.5176857E-4	9.043019	8
>>END_MODULE