FastQCFastQC Report
Wed 25 May 2016
SRR1294762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3231079
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC583641.8063315691135995No Hit
GTACATGGGAAGCAGTGGTATCAAC563661.7444946409543065No Hit
GAGTACATGGGAAGCAGTGGTATCA313090.9689951870567077No Hit
CATGTACTCTGCGTTGATACCACTG255110.7895504876234842No Hit
GCGTTGATACCACTGCTTCCCATGT193240.5980664663414296No Hit
GCTTCCCATGTACTCTGCGTTGATA186270.5764947251367113No Hit
ACGCAGAGTACATGGGAAGCAGTGG183480.5678598387721253No Hit
GTATCAACGCAGAGTACATGGGAAG161760.5006377126650262No Hit
ACTCTGCGTTGATACCACTGCTTCC136120.42128341646861617No Hit
TATCAACGCAGAGTACATGGGAAGC127950.3959977456447212No Hit
GGTATCAACGCAGAGTACATGGGAA111510.34511690986199967No Hit
CAGTGGTATCAACGCAGAGTACATG102080.315931612937969No Hit
GTATCAACGCAGAGTACTTTTTTTT100250.31026787026872443No Hit
GTGGTATCAACGCAGAGTACATGGG91840.2842394135209941No Hit
GGTATCAACGCAGAGTACTTTTTTT82180.25434228008662124No Hit
GCAGTGGTATCAACGCAGAGTACAT77690.24044599342820153No Hit
CATGGGAAGCAGTGGTATCAACGCA73180.22648780794279558No Hit
TATCAACGCAGAGTACTTTTTTTTT70810.21915279694492149No Hit
ACATGGGAAGCAGTGGTATCAACGC70380.21782197216471652No Hit
ATACCACTGCTTCCCATGTACTCTG66840.20686587978814508No Hit
GGGAAGCAGTGGTATCAACGCAGAG63810.1974882074997238No Hit
GTACATGGTAAGCAGTGGTATCAAC62570.19365048022657447No Hit
CTGCTTCCCATGTACTCTGCGTTGA60420.18699635632554945No Hit
AAGCAGTGGTATCAACGCAGAGTAC59650.1846132514865777No Hit
GTACTCTGCGTTGATACCACTGCTT57020.17647355573788198No Hit
GCAGAGTACATGGGAAGCAGTGGTA53320.16502227274542033No Hit
GATACCACTGCTTCCCATGTACTCT51280.15870859239281984No Hit
TACCACTGCTTCCCATGTACTCTGC51030.15793485705549137No Hit
CTTCCCATGTACTCTGCGTTGATAC50370.15589219576494415No Hit
CCATGTACTCTGCGTTGATACCACT50250.15552080280302646No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49090.15193067083782227No Hit
GTTGATACCACTGCTTCCCATGTAC48650.15056889664412415No Hit
CCACTGCTTCCCATGTACTCTGCGT47070.1456788893122081No Hit
ACCATGTACTCTGCGTTGATACCAC47040.14558604107172868No Hit
ATGGGAAGCAGTGGTATCAACGCAG46750.14468850808042763No Hit
GTACATGGGTAAGCAGTGGTATCAA46470.1438219245026197No Hit
GTACTTTTTTTTTTTTTTTTTTTTT36620.11333675221187722No Hit
GGTAAGCAGTGGTATCAACGCAGAG36380.11259396628804186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35290.10922048021728965No Hit
TTCCCATGTACTCTGCGTTGATACC33860.10479471408777069No Hit
CCCCATGTACTCTGCGTTGATACCA32320.10002850440982718No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTG657.917601E-411.7115415
TAAGACA4400.010.3806844
CTAAGAC2400.010.3085973
AGCACCG1201.6688542E-59.5156285
ATAACAC2500.09.5156283
TAACACG900.00109757259.5156274
CTAGGAC2303.6379788E-129.5156263
GACCGTC800.0045407799.4941267
ATTCCGT2151.07320375E-109.2751996
TACACTG5600.09.0058615
GTCTAGT1804.084177E-88.99074651
CGCACCT850.007488.93509612
CTAGACA3100.08.9017164
TAGACTG3100.08.9017165
TAACACT3100.08.9017164
TCTATAC3550.08.8455123
TCTAGAC3050.08.7356583
ACTAGAC1752.5624104E-78.7000033
GTATTAA4400.08.6541941
TCTAGTA2201.5570549E-98.6507042
Produced by FastQC (version 0.11.5)