FastQCFastQC Report
Wed 25 May 2016
SRR1294761_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294761_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893569
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42760.1477759818411104No Hit
GTACATGGGAAGCAGTGGTATCAAC40300.13927437016362837No Hit
CCCATGTACTCTGCGTTGATACCAC34900.12061229574964344No Hit
GGTATCAACGCAGAGTACTTTTTTT30610.1057863144096443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCT550.0030705712.090374
CAGGTCG951.3689418E-510.9943779
TATACTG7500.09.8783645
CGCCCTA1106.798332E-59.50532716
GTTCTAG2800.09.5035191
GTATTAC3600.09.5035191
TACACCG1201.7047843E-59.4984265
ACACCGT1606.69188E-89.4953086
CCTACAC3650.09.3702553
CTGATCG850.00746924768.9366069
CTCCTAG3300.08.9275491
GGTCTAT2151.035005E-98.8404831
TAAGACT3250.08.768844
GACCGTG1201.7743811E-48.7038817
GTCTAGA2850.08.6698771
CTAGGAC3200.08.60973553
AGGACTG5100.08.5672085
GTATTAA5350.08.5265231
GTGCTAG3700.08.4761111
TGGACTG4600.08.4659895
Produced by FastQC (version 0.11.5)