##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294761_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2893569
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334279224030947	33.0	33.0	33.0	27.0	33.0
2	31.305843406533594	33.0	33.0	33.0	27.0	33.0
3	31.298191264835918	33.0	33.0	33.0	27.0	33.0
4	34.73596897119094	37.0	37.0	37.0	27.0	37.0
5	34.94114983952344	37.0	37.0	37.0	33.0	37.0
6	35.13317636455187	37.0	37.0	37.0	33.0	37.0
7	35.15458107271677	37.0	37.0	37.0	33.0	37.0
8	35.10859357423307	37.0	37.0	37.0	33.0	37.0
9	34.79676309775229	37.0	37.0	37.0	27.0	37.0
10	34.89474348114733	37.0	37.0	37.0	33.0	37.0
11	35.1045877945195	37.0	37.0	37.0	33.0	37.0
12	35.03701898935191	37.0	37.0	37.0	33.0	37.0
13	34.97073372019123	37.0	37.0	37.0	33.0	37.0
14	36.197412952654666	40.0	37.0	40.0	27.0	40.0
15	36.51454449505092	40.0	37.0	40.0	33.0	40.0
16	36.534250263256205	40.0	37.0	40.0	33.0	40.0
17	36.473241868433064	40.0	37.0	40.0	33.0	40.0
18	36.47658756366273	40.0	37.0	40.0	33.0	40.0
19	36.57014261626386	40.0	37.0	40.0	33.0	40.0
20	36.469654948611904	40.0	37.0	40.0	27.0	40.0
21	36.420300673666326	40.0	37.0	40.0	27.0	40.0
22	36.281218108156395	40.0	37.0	40.0	27.0	40.0
23	35.88656292626856	40.0	37.0	40.0	27.0	40.0
24	36.20717598232494	40.0	37.0	40.0	27.0	40.0
25	35.14079049091278	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.40615562424253326
1101	2	0.5445283223211312
1101	3	0.38119599483986377
1101	4	0.4839633730609805
1101	5	0.399817409142301
1101	6	0.42111525953863804
1101	7	0.41165541579204756
1101	8	0.49432238480855517
1101	9	0.6713508876444365
1101	10	0.6810815470953102
1101	11	0.39419761734892944
1101	12	0.41924585662098934
1101	13	0.5208934413821424
1101	14	1.0799654159136978
1101	15	0.8630796918451438
1101	16	0.8245996797147797
1101	17	0.8793387980080922
1101	18	0.8385447717053651
1101	19	0.8493717122387139
1101	20	0.817829651392131
1101	21	0.8023234966391399
1101	22	0.9089140920345926
1101	23	1.2380784150785118
1101	24	0.9954734883191705
1101	25	0.8298514770065495
1102	1	0.2522590510081528
1102	2	0.4802497046632226
1102	3	0.2954571306568923
1102	4	0.20441968131348176
1102	5	0.24234629354210568
1102	6	0.18442269501066022
1102	7	0.26479072105647816
1102	8	0.3151863934830246
1102	9	0.591830505018109
1102	10	0.6351720473732456
1102	11	0.3030417222139903
1102	12	0.3631431581965856
1102	13	0.40592321060918124
1102	14	0.9551932427543477
1102	15	0.7779772316318088
1102	16	0.4826897201963476
1102	17	0.7379267530279847
1102	18	0.5615482923515813
1102	19	0.5784681953195303
1102	20	0.7678728206112311
1102	21	0.6954867398176319
1102	22	0.7422249732267261
1102	23	1.1122810589846779
1102	24	0.75431514060665
1102	25	0.6008336911121148
1103	1	0.2749841015890908
1103	2	0.46135068633277854
1103	3	0.2656387110554057
1103	4	0.07992312277173852
1103	5	0.17052772010010386
1103	6	0.15000008746410032
1103	7	0.1639728277773571
1103	8	0.38908225065831914
1103	9	0.6085230201309741
1103	10	0.6136346477769763
1103	11	0.38128190897135994
1103	12	0.4102394839248973
1103	13	0.368104210584562
1103	14	0.9846118298662105
1103	15	0.7418541349783112
1103	16	0.452249114510181
1103	17	0.4072818801071918
1103	18	0.5824807023724787
1103	19	0.551787620043342
1103	20	0.7098502696002456
1103	21	0.6859439873574473
1103	22	0.6787904896616439
1103	23	1.019372195188616
1103	24	0.7292308381317341
1103	25	0.5540273039900825
1104	1	0.3193468991699895
1104	2	0.4782938758952646
1104	3	0.3823530833518518
1104	4	0.27164995554732485
1104	5	0.26428290077463146
1104	6	0.26231952870173103
1104	7	0.2845331072187136
1104	8	0.45509329753517846
1104	9	0.6408250193838327
1104	10	0.6235966920669824
1104	11	0.46722177183571745
1104	12	0.4542327916349649
1104	13	0.3893363943019281
1104	14	0.9096412176873585
1104	15	0.7390627620242185
1104	16	0.6023812616653501
1104	17	0.622830710089957
1104	18	0.5421974962834071
1104	19	0.6086882006227015
1104	20	0.7184130549973275
1104	21	0.7900376858629556
1104	22	0.8312798940910326
1104	23	1.2856364377123555
1104	24	0.922281667324377
1104	25	0.6813174240200297
1105	1	0.1568191057106958
1105	2	0.26019790836453893
1105	3	0.24574319791226884
1105	4	0.09525174283182025
1105	5	0.16433488277696284
1105	6	0.2011398325534941
1105	7	0.08502421357353285
1105	8	0.19073528521217753
1105	9	-1.7661585071175168
1105	10	-0.312664409216346
1105	11	-0.028427772290292808
1105	12	0.13274950132500862
1105	13	0.05828654111129339
1105	14	-1.8738565176293775
1105	15	-0.49334542989534214
1105	16	-0.2021890286516168
1105	17	-0.03377674798699104
1105	18	-0.05165446957141029
1105	19	0.08957931707233513
1105	20	0.22957473441812226
1105	21	0.39476215245315416
1105	22	0.38606409704954814
1105	23	0.8810895413907929
1105	24	0.5460967441784703
1105	25	0.17520199905197842
1106	1	0.07321487024857376
1106	2	0.09357913731048129
1106	3	0.149994716821368
1106	4	-1.077496768428297
1106	5	-0.22291982260372833
1106	6	-0.0701053059625707
1106	7	0.05211234578042934
1106	8	0.08353016301813199
1106	9	0.3435744361400026
1106	10	0.35440291098635157
1106	11	0.24425381191798579
1106	12	0.14826831383928862
1106	13	0.13774586436874614
1106	14	0.5348889200691573
1106	15	0.33454685089056113
1106	16	0.1324280022039872
1106	17	0.1005895455060184
1106	18	0.20185180364428845
1106	19	0.17222215312240508
1106	20	0.2932699815829949
1106	21	0.45932041885283326
1106	22	0.5258381829817296
1106	23	0.6286949853123218
1106	24	0.32400433940818374
1106	25	0.2844413506790602
1107	1	0.3508175156473179
1107	2	0.32669208087967405
1107	3	0.36519371187290517
1107	4	0.5651986458049834
1107	5	0.5544983700661987
1107	6	0.29443204304131854
1107	7	0.28314090264495917
1107	8	0.29957295038170173
1107	9	0.5152538618071603
1107	10	0.48441798933655633
1107	11	0.30583828083071296
1107	12	0.2271271061590454
1107	13	0.2503736154396421
1107	14	0.7408608976858844
1107	15	0.5429197420218301
1107	16	0.5614840552171074
1107	17	0.5960384015254263
1107	18	0.5350937159429776
1107	19	0.5036400257220706
1107	20	0.6442375240876501
1107	21	0.671026859790075
1107	22	0.5204858969533319
1107	23	0.8123754995276187
1107	24	0.7246362462383544
1107	25	0.5375075310038611
1108	1	0.5383005390406588
1108	2	0.423885249517447
1108	3	0.5879620807435302
1108	4	0.745692227129183
1108	5	0.6067671249951871
1108	6	0.4285228185059893
1108	7	0.3887805253038863
1108	8	0.46543032793511685
1108	9	0.2813057748733314
1108	10	0.4778243239007125
1108	11	0.3810467134726423
1108	12	0.37415205150590936
1108	13	0.38277377598549833
1108	14	-0.9428725733553662
1108	15	0.006889308766680813
1108	16	0.38488671141524833
1108	17	0.5173865421041199
1108	18	0.7197534597984756
1108	19	0.6491555411865164
1108	20	0.7760909710858996
1108	21	0.7490849327830489
1108	22	0.85310955346295
1108	23	1.0351091863145072
1108	24	0.9614019639643416
1108	25	0.8133846172131953
1109	1	0.4655231075277939
1109	2	0.3674589753477733
1109	3	0.5017654886419898
1109	4	0.5582699471224828
1109	5	0.5849871555421515
1109	6	0.5514057756091049
1109	7	0.35432130476736745
1109	8	0.4874142359989122
1109	9	0.6001053079381862
1109	10	0.5377617130617622
1109	11	0.3692068130351345
1109	12	0.319285149259251
1109	13	0.3492621115396801
1109	14	0.787236080417216
1109	15	0.5715343089503904
1109	16	0.49091287825591223
1109	17	0.4849882813465243
1109	18	0.5248498351183315
1109	19	0.5809864480998286
1109	20	0.651733251301593
1109	21	0.6161378580548842
1109	22	0.7938729715279962
1109	23	1.2236984670988775
1109	24	0.8818092748770141
1109	25	0.6833213355679035
1110	1	0.35988916235493207
1110	2	0.2571084758973434
1110	3	0.4097498660094061
1110	4	0.6395675622972234
1110	5	0.4672061100609355
1110	6	0.29104071979617174
1110	7	0.2861662146123791
1110	8	0.2872172102954593
1110	9	0.35432041867044717
1110	10	0.506311468616893
1110	11	0.26146942152773534
1110	12	0.17672192023675137
1110	13	0.1661581377377317
1110	14	0.6254801315785343
1110	15	0.46312785374676935
1110	16	0.3726308143016226
1110	17	0.490768696965155
1110	18	0.5481714704637852
1110	19	0.4665170459327541
1110	20	0.5807874674702802
1110	21	0.6143437226159136
1110	22	0.6380484334114911
1110	23	0.8834059928421922
1110	24	0.7225216732705775
1110	25	0.6135849767026542
1111	1	0.1787230388611576
1111	2	0.19277299876264564
1111	3	0.288334658946269
1111	4	0.4611661017862403
1111	5	0.38187550981507457
1111	6	0.27393012549872964
1111	7	0.23319727380507516
1111	8	0.2608916591685002
1111	9	0.27039863260389296
1111	10	0.2581684976593266
1111	11	0.14868233900508443
1111	12	0.1510002392120242
1111	13	0.15617365511130288
1111	14	-1.6466868041744647
1111	15	-0.40149986818278194
1111	16	0.038768892073306915
1111	17	0.22757392920350838
1111	18	0.2654740549889354
1111	19	0.27058173110174266
1111	20	0.3098928118989335
1111	21	0.5126634886645149
1111	22	0.2842816229234586
1111	23	-1.932408718346501
1111	24	-0.26483330623404555
1111	25	0.0741738757524999
1112	1	-0.10398121733403798
1112	2	-0.3168014320696919
1112	3	0.054598333263328414
1112	4	0.22647871629438043
1112	5	-0.7535087259920985
1112	6	-1.0535848536818193
1112	7	-1.1609897124174395
1112	8	-0.8359009800854125
1112	9	-0.8337495802051791
1112	10	-1.0468114404579296
1112	11	-0.9757536086479419
1112	12	-1.026451078880335
1112	13	-0.9554623742036981
1112	14	-0.7735907833872844
1112	15	-1.0422271265950727
1112	16	-0.9317951270870424
1112	17	-0.9531510999835504
1112	18	-1.1335963992855156
1112	19	-1.0177550494438492
1112	20	-1.0723032770695795
1112	21	-1.9390500966323003
1112	22	-1.8916487867985268
1112	23	-1.5868155922334637
1112	24	-1.782782986624028
1112	25	-2.090897552148654
1113	1	0.1119302991110942
1113	2	-0.08235840641694736
1113	3	0.05039008570507164
1113	4	0.30160241863259785
1113	5	0.14898978305043897
1113	6	0.10998787124584197
1113	7	0.082048539320553
1113	8	0.15255222684109526
1113	9	0.38841811790823755
1113	10	0.09523374394488116
1113	11	-0.0596954104970564
1113	12	0.10601128392743675
1113	13	-0.08491256610842868
1113	14	0.1318515217318108
1113	15	0.05340741432566887
1113	16	0.1446494003288734
1113	17	0.19999600429427034
1113	18	0.21784124236694424
1113	19	0.1107369752556977
1113	20	-0.46934260569959463
1113	21	-0.35370640392265784
1113	22	0.022892658140420963
1113	23	-0.08990528832644173
1113	24	0.2411151417817834
1113	25	-0.8665594246073027
1114	1	-0.8339653664048221
1114	2	-0.677215762750695
1114	3	-0.4599124719368568
1114	4	-1.4223331394801733
1114	5	-0.6787706941191232
1114	6	-0.6243666827529637
1114	7	-0.44563840268938293
1114	8	-0.31709511344035946
1114	9	-0.39368793269922264
1114	10	-0.5349556659013786
1114	11	-0.5243004566996774
1114	12	-0.4912482815515773
1114	13	-0.4798077353301906
1114	14	-0.573038702727672
1114	15	-1.2757245981016823
1114	16	-0.7500124379228623
1114	17	-0.8051135530949693
1114	18	-0.6892216779151283
1114	19	-0.6859588509649939
1114	20	-1.9505629621224259
1114	21	-6.668027126294255
1114	22	-7.905448101425964
1114	23	-8.176213932389
1114	24	-5.96031429446078
1114	25	-7.277673959481511
1115	1	-1.020585013590388
1115	2	-1.0448278688643526
1115	3	-0.8509378108362213
1115	4	-0.6602971622829443
1115	5	-0.6968182073608844
1115	6	-0.9438068254946828
1115	7	-1.0278473083362059
1115	8	-0.8948798113486092
1115	9	-2.777855609330878
1115	10	-1.5409683241292242
1115	11	-1.0849034057974407
1115	12	-1.0699834687317562
1115	13	-1.4449583323317228
1115	14	-1.1655021195514408
1115	15	-1.8921320404214583
1115	16	-1.262680204329179
1115	17	-3.0781138224189633
1115	18	-2.888948426276741
1115	19	-1.8866804148396668
1115	20	-1.628333632739178
1115	21	-1.2125037399938705
1115	22	-0.7809149296870288
1115	23	-0.14710561925496535
1115	24	-1.2368601019838934
1115	25	-0.9096943383306026
1116	1	-1.2835434254765516
1116	2	-0.758239524687184
1116	3	-0.544900257857357
1116	4	-0.08140486943798209
1116	5	-0.15223283047266278
1116	6	-0.27139088883779294
1116	7	-0.6371662729980656
1116	8	-1.5182276917215063
1116	9	-0.7309186677647332
1116	10	-2.4120820261056863
1116	11	-1.0299744518140415
1116	12	-0.6780681793974566
1116	13	-0.673956080877609
1116	14	-0.9857413454103252
1116	15	-1.0696286620590385
1116	16	-0.6309584738988789
1116	17	-0.37954171210007814
1116	18	-1.0560771076286528
1116	19	-0.6776421731946201
1116	20	-0.8815534129329947
1116	21	-0.41632825887253944
1116	22	0.1068992225802532
1116	23	0.1031775843775975
1116	24	0.059183817908753156
1116	25	0.02153767720513855
1201	1	0.283699404042153
1201	2	0.3762778963024971
1201	3	0.30816723831525294
1201	4	0.2937734866391395
1201	5	0.24959795544311447
1201	6	0.23448322993021264
1201	7	0.2881183013790576
1201	8	0.31228248671268233
1201	9	0.6449636411236739
1201	10	0.576903842264791
1201	11	0.35677309157799897
1201	12	0.3856559499864147
1201	13	0.3397730748871055
1201	14	0.9892023666495149
1201	15	0.658093143927637
1201	16	0.6366942041708512
1201	17	0.5358188091530067
1201	18	0.40651922333649537
1201	19	0.476039693590252
1201	20	0.6144037628211976
1201	21	0.6955100383297577
1201	22	0.7184935703436324
1201	23	0.4824518310341759
1201	24	0.5028502329190374
1201	25	0.5330544728684075
1202	1	-0.21347762629682876
1202	2	0.074950003972031
1202	3	0.023611177409577522
1202	4	-0.06646284943267489
1202	5	-0.09030472970378867
1202	6	-0.22035824393439896
1202	7	-0.1181724087676912
1202	8	0.1006736157207726
1202	9	0.21813674051785625
1202	10	0.2858964777355766
1202	11	0.15367747254407504
1202	12	0.005292097900195358
1202	13	0.09652668973841116
1202	14	0.3779512356040371
1202	15	0.32824253181481566
1202	16	0.2609078654025012
1202	17	0.09660224165880038
1202	18	0.1589961380242073
1202	19	0.20804693228129167
1202	20	0.4383795299846227
1202	21	0.3389481768835765
1202	22	0.44124571109063027
1202	23	0.6147335167862948
1202	24	0.3074559100182839
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2211	5	0.40142916609241297
2211	6	0.21528337537382214
2211	7	0.2637036678673823
2211	8	0.3695226716672835
2211	9	0.54492241537762
2211	10	0.4016490195878859
2211	11	0.2808798428952599
2211	12	0.25846441495379935
2211	13	0.22919845213022683
2211	14	0.5700716528981076
2211	15	0.3949158711044447
2211	16	0.37188408486115065
2211	17	0.3478039761482634
2211	18	0.3226030145546801
2211	19	0.40372606546831946
2211	20	0.5487152267021358
2211	21	0.45707254181914436
2211	22	0.6149623473625212
2211	23	0.8129954456944049
2211	24	0.5854346351449777
2211	25	0.703212276327605
2212	1	-0.2345574004273736
2212	2	-0.17460599816897115
2212	3	-0.1765086840982697
2212	4	-0.473009090026423
2212	5	-0.09320040567818211
2212	6	-0.4515490528150039
2212	7	-0.4074570722489099
2212	8	-0.23325739659868816
2212	9	-0.1759346747925008
2212	10	-0.47442198039377814
2212	11	-0.4343774162094647
2212	12	-0.46611213829211806
2212	13	-0.4941369239340574
2212	14	-0.310073796342742
2212	15	-0.49300324616127966
2212	16	-0.31088277215431503
2212	17	-0.42862600005756235
2212	18	-0.31715674185480225
2212	19	-0.43242315499829687
2212	20	-0.35917796608242014
2212	21	-0.3497276248802521
2212	22	-0.3491674311157098
2212	23	0.1754764844226031
2212	24	-0.09524911667369906
2212	25	-0.5755942250271957
2213	1	-0.16313871564378246
2213	2	-1.225198399108308
2213	3	-0.2899816172303389
2213	4	0.005886093073335985
2213	5	0.02019234739015019
2213	6	2.5700219183022455E-5
2213	7	0.06783034938166566
2213	8	0.18714550334449598
2213	9	0.1736765933351947
2213	10	-0.4558636120873416
2213	11	-0.09727701799156563
2213	12	0.0353576344578741
2213	13	-0.35279976042573935
2213	14	-0.02445982628346144
2213	15	-0.1330791831194631
2213	16	0.054548500509106645
2213	17	0.16413711516226215
2213	18	0.15914136801237078
2213	19	0.15372237376825382
2213	20	0.20799912504924833
2213	21	-0.3533731841288841
2213	22	-0.9201943845903031
2213	23	-0.018999626161502192
2213	24	0.09120137920878335
2213	25	-0.4548911375582563
2214	1	-0.24524998458215563
2214	2	-0.4860366558618878
2214	3	-0.4602680557491965
2214	4	-0.47975167396069196
2214	5	-0.20570873012219693
2214	6	-0.16377803259914003
2214	7	-0.5442211918769928
2214	8	-0.1300317979817578
2214	9	-0.024402852454016966
2214	10	-1.9563141454548756
2214	11	-0.9305737229463134
2214	12	-0.6152904895411808
2214	13	-0.2997180837397906
2214	14	-0.8404281755707643
2214	15	-0.6244068361083279
2214	16	-0.7768026469477789
2214	17	-0.5167921449344348
2214	18	-0.6648544590252357
2214	19	-1.4280903214352136
2214	20	-2.5988967384155615
2214	21	-1.8651878407971054
2214	22	-1.9922315705134537
2214	23	-2.500766065283891
2214	24	-1.645319844486572
2214	25	-1.9358108705403936
2215	1	-0.13179625442944243
2215	2	-0.2706066630086248
2215	3	-0.7386714405497585
2215	4	-0.1447501383678116
2215	5	0.0022241013112491714
2215	6	-0.018989845388318827
2215	7	-0.10453597037179208
2215	8	-0.0793675319278151
2215	9	-1.2327613856655262E-4
2215	10	-0.14538889286986745
2215	11	-0.5217916809237977
2215	12	-0.27999701965319446
2215	13	-0.21370415275670496
2215	14	-0.03603537645826549
2215	15	-0.5124227682475322
2215	16	-0.3282038567432153
2215	17	-0.12884874524183232
2215	18	-0.09000858827365477
2215	19	-2.2278147685579555
2215	20	-2.339923907010281
2215	21	-3.3208923460743236
2215	22	-2.2236126719204137
2215	23	-3.2749218567048644
2215	24	-2.331059903866354
2215	25	-1.4925164556180022
2216	1	-1.5085965447440337
2216	2	-1.251355970224008
2216	3	-0.8687909454621412
2216	4	-0.8395024189665037
2216	5	-1.778936238136204
2216	6	-1.362737238380042
2216	7	-1.0598702117942125
2216	8	-4.940758046463969
2216	9	-3.909931836652646
2216	10	-1.753449388833836
2216	11	-0.9570478116934353
2216	12	-1.0313580081071905
2216	13	-1.9226500795357424
2216	14	-3.448688574848802
2216	15	-1.953034338901567
2216	16	-2.990013917135812
2216	17	-2.646270142395146
2216	18	-2.5194692543342825
2216	19	-1.5420126669235117
2216	20	-0.7652903988746544
2216	21	-0.6086042202251889
2216	22	-0.6429495281359863
2216	23	-0.937428877553117
2216	24	-0.5093203455374535
2216	25	-0.2289570397507319
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	35.0
8	184.0
9	539.0
10	1087.0
11	2119.0
12	3846.0
13	5682.0
14	7863.0
15	10107.0
16	11760.0
17	12875.0
18	13461.0
19	13897.0
20	14194.0
21	15245.0
22	16277.0
23	17487.0
24	18470.0
25	20600.0
26	22309.0
27	25484.0
28	29461.0
29	35090.0
30	42301.0
31	53066.0
32	72029.0
33	104934.0
34	154502.0
35	249934.0
36	443755.0
37	1474975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.439526116649006	19.949804593985885	12.643953130212484	23.966716159152625
2	15.919127901972963	20.22856660932454	39.6655422909406	24.18676319776189
3	19.196977778207938	24.496754374654984	28.9711856772847	27.335082169852377
4	12.850666939517083	16.1332717576314	37.07197167148995	33.94408963136157
5	13.809203162710054	37.48237965102895	34.735471810552646	13.972945375708356
6	32.8257299973284	37.261617038219036	16.358603594869844	13.554049369582716
7	27.644935249491287	32.21272145669291	21.84358549721313	18.298757796602672
8	24.756631968924093	37.392070667274915	19.929360372689697	17.921936991111295
9	24.392244982735793	17.09548690729997	21.091638359542696	37.42062975042154
10	15.134710894892015	29.98617277567791	32.89687088634892	21.98224544308115
11	33.871738087634974	25.002419175351502	22.18269750493166	18.94314523208186
12	21.270263227559855	26.71369553301801	30.677729924086123	21.338311315336014
13	27.212554993761902	23.132596742364807	26.111712072880344	23.543136190992946
14	20.68600735081932	22.518701044217234	27.29566225629027	29.499629348673174
15	23.347876183939412	30.301072949952275	23.18990239563051	23.161148470477805
16	22.70309481934244	30.318025182537628	24.892544115060137	22.086335883059796
17	21.140103196463812	29.633455560893175	27.463599572837143	21.762841669805876
18	21.4521833103567	29.22113177760367	28.135982518172508	21.190702393867124
19	22.033273890985498	28.192710709299273	28.261000290299837	21.51301510941539
20	22.803137175753864	27.906024347878976	27.808566477672127	21.482271998695033
21	22.86995670792044	27.95930952643646	27.491975038140403	21.678758727502697
22	22.201662309310173	27.691966081447	28.205787127426994	21.900584481815834
23	22.18618645207994	27.985105471207568	28.048537957287177	21.780170119425314
24	22.12050144770665	27.619471222467133	27.918343677057393	22.341683652768822
25	22.607457746881348	27.57582527584242	27.425049314788545	22.391667662487684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2848.0
1	2848.0
2	2867.5
3	2887.0
4	2887.0
5	2887.0
6	3938.5
7	4990.0
8	4990.0
9	4990.0
10	7780.0
11	10570.0
12	10570.0
13	10570.0
14	17388.5
15	24207.0
16	24207.0
17	24207.0
18	37295.5
19	50384.0
20	50384.0
21	50384.0
22	71967.5
23	93551.0
24	93551.0
25	93551.0
26	121622.5
27	149694.0
28	149694.0
29	149694.0
30	179864.0
31	210034.0
32	210034.0
33	210034.0
34	238484.5
35	266935.0
36	266935.0
37	266935.0
38	288819.5
39	310704.0
40	310704.0
41	310704.0
42	316462.0
43	322220.0
44	322220.0
45	322220.0
46	326474.5
47	330729.0
48	330729.0
49	330729.0
50	320086.5
51	309444.0
52	309444.0
53	309444.0
54	289544.0
55	269644.0
56	269644.0
57	269644.0
58	241011.0
59	212378.0
60	212378.0
61	212378.0
62	182557.0
63	152736.0
64	152736.0
65	152736.0
66	121964.0
67	91192.0
68	91192.0
69	91192.0
70	68763.5
71	46335.0
72	46335.0
73	46335.0
74	33511.0
75	20687.0
76	20687.0
77	20687.0
78	14209.0
79	7731.0
80	7731.0
81	7731.0
82	5160.0
83	2589.0
84	2589.0
85	2589.0
86	1691.0
87	793.0
88	793.0
89	793.0
90	506.0
91	219.0
92	219.0
93	219.0
94	135.5
95	52.0
96	52.0
97	52.0
98	34.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03020491303300526
2	0.014100234001677514
3	0.02066651944363518
4	0.02256728628209661
5	0.020977554017201593
6	0.005805978706573093
7	3.455939706293508E-5
8	3.1103457356641573E-4
9	3.455939706293508E-4
10	6.911879412587016E-5
11	7.257473383216367E-4
12	9.331037206992473E-4
13	0.0020390044267131697
14	4.8383155888109114E-4
15	0.0017625292502096892
16	0.0019353262355243646
17	0.0020390044267131697
18	0.001727969853146754
19	3.1103457356641573E-4
20	3.801533676922859E-4
21	0.07872630650936611
22	0.07914101927412134
23	0.0796939696271283
24	9.331037206992473E-4
25	0.22477431849732976
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2893569.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.87698623684587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.12933179836088	55.87943869680204
2	12.089016695393532	16.653100730912406
3	3.4704976771824643	7.171122622389064
4	1.3121968715683343	3.6152066345217753
5	0.6432575090732756	2.215281929959388
6	0.325441689102993	1.3449265644745634
7	0.21645407576039866	1.043609307794072
8	0.14800554022400791	0.8155340445588746
9	0.108861132096089	0.6748224027398805
>10	0.513150787698675	6.232268656148691
>50	0.03147679439283633	1.4744971188500557
>100	0.010899115808441048	1.3799969131168237
>500	5.036833353219184E-4	0.24249840912756543
>1k	9.066300028055755E-4	1.2576959686046696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4276	0.1477759818411104	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4030	0.13927437016362837	No Hit
CCCATGTACTCTGCGTTGATACCAC	3490	0.12061229574964344	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3061	0.1057863144096443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.455939706293508E-5	0.0	0.0	0.0
6	0.0	3.455939706293508E-5	0.0	0.0	0.0
7	0.0	3.455939706293508E-5	0.0	0.0	0.0
8	0.0	3.455939706293508E-5	0.0	0.0	0.0
9	0.0	3.455939706293508E-5	0.0	0.0	0.0
10	0.0	3.455939706293508E-5	0.0	6.911879412587016E-5	0.0
11	0.0	3.455939706293508E-5	0.0	6.911879412587016E-5	0.0
12	0.0	3.455939706293508E-5	0.0	1.0367819118880524E-4	0.0
13	0.0	6.911879412587016E-5	0.0	1.0367819118880524E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCT	55	0.00307057	12.09037	4
CAGGTCG	95	1.3689418E-5	10.994377	9
TATACTG	750	0.0	9.878364	5
CGCCCTA	110	6.798332E-5	9.505327	16
GTTCTAG	280	0.0	9.503519	1
GTATTAC	360	0.0	9.503519	1
TACACCG	120	1.7047843E-5	9.498426	5
ACACCGT	160	6.69188E-8	9.495308	6
CCTACAC	365	0.0	9.370255	3
CTGATCG	85	0.0074692476	8.936606	9
CTCCTAG	330	0.0	8.927549	1
GGTCTAT	215	1.035005E-9	8.840483	1
TAAGACT	325	0.0	8.76884	4
GACCGTG	120	1.7743811E-4	8.703881	7
GTCTAGA	285	0.0	8.669877	1
CTAGGAC	320	0.0	8.6097355	3
AGGACTG	510	0.0	8.567208	5
GTATTAA	535	0.0	8.526523	1
GTGCTAG	370	0.0	8.476111	1
TGGACTG	460	0.0	8.465989	5
>>END_MODULE