FastQCFastQC Report
Wed 25 May 2016
SRR1294761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893569
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT131350.4539376804216523No Hit
GGTATCAACGCAGAGTACTTTTTTT107980.373172369485573No Hit
TATCAACGCAGAGTACTTTTTTTTT84880.29334016227019294No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55090.19038771841970936No Hit
GTACATGGGAAGCAGTGGTATCAAC41810.14449283912013158No Hit
CCCATGTACTCTGCGTTGATACCAC38330.13246616894223015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACA250.006057034218.98709312
TATCGCG759.749765E-713.9245897
CGCGGCG658.071811E-411.684566510
TCGCGCG907.5219123E-611.6034239
GTGTAGG3850.011.3763571
CTTAGAC2750.011.07286553
GGACCGC1303.2934622E-810.9561736
CTAGGAC2700.010.9254843
AGTACCG700.001471603810.8751365
TCTACGC909.362146E-510.573053
TGTATCG1201.4928755E-610.30872255
TACACCG951.6176749E-410.0165735
GGTATCA38450.09.5834251
TCGTAGG800.00445614349.5157442
TAGGCCG900.00109737589.5157445
ATACCGT1106.876883E-59.4953516
ATCGCGC1201.7141343E-59.4940398
CGCGCGC1106.889896E-59.493710510
TAGGACA3550.09.381724
GCAGGAC4100.09.2836523
Produced by FastQC (version 0.11.5)