FastQCFastQC Report
Wed 25 May 2016
SRR1294760_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294760_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330216
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC42340.18169989391541386No Hit
CCCATGTACTCTGCGTTGATACCAC36270.15565080662050212No Hit
GTATCAACGCAGAGTACTTTTTTTT32210.1382275291217638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG500.001503134613.2962445
TACATCG752.0801109E-411.3967815
CGGTACA600.005888577611.0804414
TATTCCG951.3651581E-510.9968935
GCTATCG700.001497012810.85267911
CCGCTAT700.001497012810.8526799
GTCAGCG700.001497012810.85267912
GTCTTAC1502.1336746E-910.7668611
TAGGACC1252.249526E-710.6372234
TAGGGTG2750.010.360715
ATCGGTC2200.010.35893214
TATCGGT2201.8189894E-129.92730913
GTCTAGA2151.0913936E-119.7211061
CTATCGG2251.8189894E-129.70711912
GTCCTAC1701.6651029E-89.5001721
CGCTATC800.0045322259.49609410
TCCGCTA800.00453302739.4958918
TACACTG5850.09.4161435
TCGGTCA2357.2759576E-129.30343315
CGAGACT1356.8720583E-69.1457614
Produced by FastQC (version 0.11.5)