FastQCFastQC Report
Wed 25 May 2016
SRR1294760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330216
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98150.4212055878081688No Hit
GGTATCAACGCAGAGTACTTTTTTT76630.3288536341695362No Hit
TATCAACGCAGAGTACTTTTTTTTT56440.24220930591842127No Hit
GTACATGGGAAGCAGTGGTATCAAC43330.18594842709860374No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39260.16848223512326754No Hit
CCCATGTACTCTGCGTTGATACCAC39180.16813891931048452No Hit
GAGTACATGGGAAGCAGTGGTATCA24660.10582709929036621No Hit
ATTCCATTCCATTCCATTCCATTCC24110.10346680307748293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG401.269538E-519.0278225
GTGCGGA456.794893E-414.76893311
GTTCGCC400.00530116514.2414711
AGCGTCA1155.2150426E-912.4094513
TAGACAG3100.012.2760155
AGGACCG600.005813480411.0995645
GGCCCGT600.005897744611.0781256
GCAGCGT1355.540278E-810.5748781
CAGCGTC1355.5701094E-810.57101252
GACCACG909.572615E-510.549697
CACACCG750.00261646310.1481725
CCTAGAC2300.09.927563
TATACTG5550.09.5996235
CTAGACA2500.09.5139114
CTGAGCG1002.7715895E-49.4947219
AGCGCCA1002.7727842E-49.49431410
AGGCGTG2451.8189894E-129.3009527
CTTAGAC2151.03682396E-109.2926583
GCTACAC1856.53381E-99.2567793
CTAGACC1901.0319127E-89.013184
Produced by FastQC (version 0.11.5)