FastQCFastQC Report
Wed 25 May 2016
SRR1294759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926251
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC72230.2468346016797602No Hit
CCCATGTACTCTGCGTTGATACCAC65560.2240409315537184No Hit
GTATCAACGCAGAGTACTTTTTTTT41480.14175133985430505No Hit
GAGTACATGGGAAGCAGTGGTATCA36970.12633912811990494No Hit
GGTATCAACGCAGAGTACTTTTTTT30600.10457066054825781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCC604.1068878E-412.66192811
TACCGTA752.0822328E-411.3957347
TACACCG1104.990816E-711.2242235
GTCGTAC600.005870204411.0852481
TAGCACG700.001493977110.8554934
AGACCGT803.7870833E-410.6831366
ACCGTAT750.002657001810.1295418
ACCGCCC856.6193833E-410.0550598
CGCCCTA1803.9108272E-1010.0327716
TCTACAC3900.09.9861743
ACACCGT1054.126047E-59.9483176
CTAGGAC2500.09.87900453
GTCTTAC2950.09.6626861
GTACGGA1201.6979904E-59.5016411
TTAGACT2106.730261E-119.4985564
TAGCGTG800.0045275969.4974195
CCGTTCA800.00453078849.4966089
AACCGTG800.00453142739.4964467
CTTAGAC2451.8189894E-129.3051853
CCCGTAC2357.2759576E-129.30250116
Produced by FastQC (version 0.11.5)