##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294759_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2926251
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17271792474398	33.0	33.0	33.0	27.0	33.0
2	31.12183472983008	33.0	33.0	33.0	27.0	33.0
3	31.127416274270388	33.0	33.0	33.0	27.0	33.0
4	34.5744736182918	37.0	37.0	37.0	27.0	37.0
5	34.81096529313446	37.0	37.0	37.0	27.0	37.0
6	35.00968098772115	37.0	37.0	37.0	33.0	37.0
7	34.95049638599013	37.0	37.0	37.0	33.0	37.0
8	34.91182403696744	37.0	37.0	37.0	33.0	37.0
9	34.54933462645549	37.0	37.0	37.0	27.0	37.0
10	34.637798329671654	37.0	37.0	37.0	27.0	37.0
11	34.898290679781056	37.0	37.0	37.0	33.0	37.0
12	34.82615332724363	37.0	37.0	37.0	33.0	37.0
13	34.760596066434495	37.0	37.0	37.0	27.0	37.0
14	35.90818217576004	40.0	37.0	40.0	27.0	40.0
15	36.24366980139435	40.0	37.0	40.0	27.0	40.0
16	36.25625518795209	40.0	37.0	40.0	27.0	40.0
17	36.18411783541467	40.0	37.0	40.0	27.0	40.0
18	36.19056055000067	40.0	37.0	40.0	27.0	40.0
19	36.27888721780872	40.0	37.0	40.0	27.0	40.0
20	36.18846503597948	40.0	37.0	40.0	27.0	40.0
21	36.133795084563836	40.0	37.0	40.0	27.0	40.0
22	35.989598636617295	40.0	37.0	40.0	27.0	40.0
23	35.59867420805666	37.0	37.0	40.0	27.0	40.0
24	35.925760982226066	37.0	37.0	40.0	27.0	40.0
25	34.81080536153597	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5486702558612429
1101	2	0.6612000601609793
1101	3	0.4567108592244544
1101	4	0.5866107512032173
1101	5	0.5684217839926546
1101	6	0.44321194597790736
1101	7	0.4804589376119708
1101	8	0.6360538930671993
1101	9	0.8380592912258464
1101	10	0.7079757414762256
1101	11	0.41927674880773225
1101	12	0.5230013901769865
1101	13	0.5377242982676478
1101	14	1.198116984662711
1101	15	0.9827517130886321
1101	16	0.8935967596603902
1101	17	0.9442392786709419
1101	18	0.9299477952050523
1101	19	0.843391857885571
1101	20	1.0032210602960134
1101	21	0.8809865049081225
1101	22	1.0112944374146835
1101	23	1.3546479594410883
1101	24	1.1760268536577172
1101	25	0.9772916889554537
1102	1	0.3100126495820561
1102	2	0.5317450101450127
1102	3	0.32645163857733195
1102	4	0.28165199163994004
1102	5	0.23795924984602834
1102	6	0.31943698108111107
1102	7	0.314849811147873
1102	8	0.42094773365563043
1102	9	0.6364517954320519
1102	10	0.7448762148735568
1102	11	0.35923368844583337
1102	12	0.4661587533297791
1102	13	0.43444078037287426
1102	14	1.052311657473986
1102	15	0.8884341720232456
1102	16	0.5407722336159466
1102	17	0.7751871442639953
1102	18	0.7667200717287486
1102	19	0.7527296674327317
1102	20	0.7777546055601192
1102	21	0.8690898957354776
1102	22	0.8335703432745802
1102	23	1.2818892693796542
1102	24	0.983925242216408
1102	25	0.7645367991666134
1103	1	0.3929371707947844
1103	2	0.5513059691458615
1103	3	0.27871658707351443
1103	4	0.2745189425295109
1103	5	0.30446401454051397
1103	6	0.2489572412426071
1103	7	0.14969233887750022
1103	8	0.5505695226574261
1103	9	0.7018058178107083
1103	10	0.6650953063554041
1103	11	0.4592300088555561
1103	12	0.4787366855253765
1103	13	0.47240381489830696
1103	14	1.0724082191352124
1103	15	0.9095953510925199
1103	16	0.6028357810505511
1103	17	0.6498571393798329
1103	18	0.6745445142453832
1103	19	0.72584327084585
1103	20	0.9621600841968885
1103	21	0.9726177122864286
1103	22	0.8650219491372297
1103	23	1.3239164033317934
1103	24	0.9086643916697383
1103	25	0.8610028553212317
1104	1	0.3290281060237916
1104	2	0.5130709505579603
1104	3	0.3202330220294982
1104	4	0.3182378267424113
1104	5	0.25013853513266326
1104	6	0.2435138675798001
1104	7	0.22211448602674722
1104	8	0.34073609927519044
1104	9	0.5282041319657438
1104	10	0.5387333200214144
1104	11	0.3925209902088227
1104	12	0.3061014252605432
1104	13	0.35866429650396014
1104	14	0.8415424868767403
1104	15	0.5735916904101472
1104	16	0.4924118490156033
1104	17	0.5515899393478136
1104	18	0.4739090882508705
1104	19	0.5335592206193098
1104	20	0.7095897197467593
1104	21	0.7424891427305624
1104	22	0.8675255540974334
1104	23	1.29439300835611
1104	24	0.9895327963977181
1104	25	0.8861760551178222
1105	1	0.26323594462299127
1105	2	0.36271308642927735
1105	3	0.382455034119932
1105	4	0.2888301887680669
1105	5	0.3192366000177884
1105	6	0.1443311017323552
1105	7	0.16505192341755048
1105	8	0.3371296869302114
1105	9	-1.6990940990561043
1105	10	-0.11626353488712482
1105	11	0.08894109425160224
1105	12	0.2786594885758049
1105	13	0.25424205768540276
1105	14	-1.6183196126060722
1105	15	-0.255952461823604
1105	16	0.03884788890249524
1105	17	0.2912978551398453
1105	18	0.12256220723141809
1105	19	0.30597379248369094
1105	20	0.44787939896579587
1105	21	0.5532231845854483
1105	22	0.6116979543782932
1105	23	1.007540469920734
1105	24	0.7298708015421695
1105	25	0.3784276238765898
1106	1	0.23846895964856785
1106	2	0.22966708744421638
1106	3	0.27262117053113855
1106	4	-0.8900474925364321
1106	5	-0.029682150016007824
1106	6	0.050218134534048886
1106	7	0.14442178065409195
1106	8	0.3331862547214257
1106	9	0.4700712178342883
1106	10	0.5398973538102112
1106	11	0.4109922289519403
1106	12	0.15927191238778704
1106	13	0.2987730113959941
1106	14	0.72399987074823
1106	15	0.4871408160202151
1106	16	0.4992631797242453
1106	17	0.230865880388194
1106	18	0.3096805140596999
1106	19	0.2910684004074753
1106	20	0.357550594238063
1106	21	0.5874019142741176
1106	22	0.6808536162166661
1106	23	0.9198699713570164
1106	24	0.4839740827922938
1106	25	0.3752878009579703
1107	1	0.4673426892016792
1107	2	0.3963546795743831
1107	3	0.5056449620081196
1107	4	0.6758653924339697
1107	5	0.5718660242760549
1107	6	0.4981827069493221
1107	7	0.5039779748237905
1107	8	0.5851976567739783
1107	9	0.7723427931496829
1107	10	0.747133844098471
1107	11	0.3855127645818044
1107	12	0.41226479618016043
1107	13	0.4107750822418623
1107	14	0.9628496212702018
1107	15	0.7230668917827927
1107	16	0.815274879383395
1107	17	0.9029406168436083
1107	18	0.8587876167820241
1107	19	0.7729021530251003
1107	20	0.8828395593886427
1107	21	0.8970191022948342
1107	22	0.8343147653811158
1107	23	1.0834860154523938
1107	24	1.0123060753459256
1107	25	0.8816049658831702
1108	1	0.49601850930584845
1108	2	0.4868589936816541
1108	3	0.49212305067525364
1108	4	0.7744123278845194
1108	5	0.5894719022214616
1108	6	0.47564145968114246
1108	7	0.45020316775052294
1108	8	0.5591613910798259
1108	9	0.27923088534249985
1108	10	0.5343397305196405
1108	11	0.4038574470082992
1108	12	0.4528731444589056
1108	13	0.45527080639327266
1108	14	-0.7868153798687274
1108	15	0.0812057189836537
1108	16	0.48566949114905356
1108	17	0.5120927398175894
1108	18	0.6599828767085327
1108	19	0.6335003656974294
1108	20	0.8140155545998269
1108	21	0.7410835040085857
1108	22	0.8345722757065488
1108	23	1.2674789970452593
1108	24	1.0453328487680338
1108	25	0.8341306565847262
1109	1	0.5864879392715565
1109	2	0.45740433643618417
1109	3	0.5349897113022095
1109	4	0.6706332132423611
1109	5	0.6836864218740573
1109	6	0.39930242107182323
1109	7	0.44251285261454143
1109	8	0.5394754035280087
1109	9	0.6994257654307887
1109	10	0.7463776621549911
1109	11	0.5350504718549516
1109	12	0.4751418587001126
1109	13	0.4882115210036133
1109	14	0.9410259618024099
1109	15	0.7135160903489677
1109	16	0.5942912535029379
1109	17	0.648783308358837
1109	18	0.7700965601987946
1109	19	0.7624506320543105
1109	20	0.8686193242422533
1109	21	0.7927816475102389
1109	22	0.8269965650229523
1109	23	1.246066533745413
1109	24	0.9863419419091954
1109	25	0.8821117519144295
1110	1	0.3710794593612903
1110	2	0.3141726790223096
1110	3	0.3572639954567407
1110	4	0.5261316480433749
1110	5	0.5040774236755539
1110	6	0.3205054370696274
1110	7	0.29115812412455
1110	8	0.3225433271869065
1110	9	0.4181377813571103
1110	10	0.4323327667247838
1110	11	0.2447483256114822
1110	12	0.2197427025226446
1110	13	0.26248281489991854
1110	14	0.7897303647809863
1110	15	0.5236015572893962
1110	16	0.41632123132680476
1110	17	0.528573884102542
1110	18	0.6410798881453061
1110	19	0.5773554967526309
1110	20	0.6747060027370466
1110	21	0.6312325531507881
1110	22	0.7198175287578792
1110	23	0.8489365244244311
1110	24	0.7174047000773243
1110	25	0.6762533703170988
1111	1	0.17948776518090526
1111	2	0.13468270228586476
1111	3	0.29059512064104354
1111	4	0.5138591202619338
1111	5	0.36178734783236166
1111	6	0.22632587887632383
1111	7	0.17253139800340023
1111	8	0.3146201209329931
1111	9	0.2926768937421542
1111	10	0.17292506580054834
1111	11	0.04516502932963817
1111	12	0.13932245261874243
1111	13	0.1350929492462143
1111	14	-1.625959786073544
1111	15	-0.5023222617482759
1111	16	-0.04649527202646908
1111	17	0.15097564866162116
1111	18	0.36588238280989316
1111	19	0.2956722632909816
1111	20	0.32729055004438123
1111	21	0.3944781754579836
1111	22	0.21182740572782421
1111	23	-1.8310900414908815
1111	24	-0.47380131502821143
1111	25	-0.01960661020577703
1112	1	-0.12087355525449439
1112	2	-0.19799560259101057
1112	3	-0.07203491435360476
1112	4	0.1419156640201109
1112	5	-0.638225221066449
1112	6	-0.8807694592305921
1112	7	-0.958676044767401
1112	8	-0.8091394084166197
1112	9	-0.6455886141546543
1112	10	-0.9128216436263372
1112	11	-0.8541289703134822
1112	12	-0.7658135180826235
1112	13	-1.0084801213747099
1112	14	-0.6928729742827073
1112	15	-0.8922568574475207
1112	16	-0.8316681846687288
1112	17	-0.968079350715243
1112	18	-1.0509773167686376
1112	19	-0.8494993145696341
1112	20	-0.9826536705989213
1112	21	-1.8345492449224707
1112	22	-1.7186270183829322
1112	23	-1.2876918467388023
1112	24	-1.5342666581485247
1112	25	-1.8031477788372001
1113	1	0.10210860964704338
1113	2	-0.04961679445422362
1113	3	-0.06495913251896113
1113	4	0.22998613130339152
1113	5	0.13773713316902558
1113	6	0.020887431874193396
1113	7	0.028515352490202872
1113	8	0.08340467901769699
1113	9	0.3185923350865423
1113	10	-0.013681276758738647
1113	11	-0.012955175000172403
1113	12	-0.027152244511853496
1113	13	-0.17526389643025908
1113	14	0.008088651622706777
1113	15	-0.09432687329898926
1113	16	0.10313427672380016
1113	17	-0.016191815856124947
1113	18	0.10882206084344404
1113	19	-0.0834741716420524
1113	20	-0.6741851711616107
1113	21	-0.5017896762815894
1113	22	-0.13852930792499052
1113	23	-0.3368846312327136
1113	24	0.027453801780616516
1113	25	-1.1728352309367693
1114	1	-0.9994272484696864
1114	2	-0.7951456229751557
1114	3	-0.6293938203815514
1114	4	-1.6213288466568017
1114	5	-0.8297400047526864
1114	6	-0.6530256038405398
1114	7	-0.5794854910327203
1114	8	-0.43456580553658597
1114	9	-0.413289420998197
1114	10	-0.5457538968687956
1114	11	-0.5239985578627966
1114	12	-0.6181125500085685
1114	13	-0.7344037994572616
1114	14	-0.5980285502096123
1114	15	-1.4482435766599764
1114	16	-0.8035340362767727
1114	17	-0.8635513069711038
1114	18	-0.8462577542100505
1114	19	-0.7888075435445998
1114	20	-2.187073805065161
1114	21	-6.782373899831594
1114	22	-7.93930897737971
1114	23	-8.130300349114652
1114	24	-6.113513181903539
1114	25	-7.40480707112739
1115	1	-1.2375061114618475
1115	2	-1.2772978915419664
1115	3	-1.0495890351581458
1115	4	-0.965197831455562
1115	5	-0.9081216432458064
1115	6	-1.2084794798692684
1115	7	-1.1884649004173014
1115	8	-0.8856732024789338
1115	9	-2.8284778047076706
1115	10	-1.8730993956798443
1115	11	-1.3558310053293923
1115	12	-1.2450232549758127
1115	13	-1.577392107354811
1115	14	-1.3245937792320817
1115	15	-2.3952096682057586
1115	16	-1.6625665679210044
1115	17	-3.268980020802651
1115	18	-3.0416999386940375
1115	19	-2.3447690322766377
1115	20	-2.0404646876680346
1115	21	-1.5402205176958077
1115	22	-1.040235600620285
1115	23	-0.5095120891433282
1115	24	-1.7038446796761235
1115	25	-1.4709625495242449
1116	1	-2.380959517720761
1116	2	-1.5454529382277222
1116	3	-1.3620343404723805
1116	4	-1.0136717537671274
1116	5	-1.0448087598346945
1116	6	-1.0328283626271926
1116	7	-1.4403555993747617
1116	8	-2.5974315854084296
1116	9	-1.6828989696892762
1116	10	-3.2644949159445673
1116	11	-1.9416549303626027
1116	12	-1.6457584691915557
1116	13	-1.6457655738848018
1116	14	-2.171105013463219
1116	15	-2.374505542603927
1116	16	-1.8143787776989626
1116	17	-1.607652156954508
1116	18	-2.17777523247765
1116	19	-1.9113617624618797
1116	20	-2.250166929250412
1116	21	-1.6243309002201443
1116	22	-1.1996337352635749
1116	23	-0.9734103564794836
1116	24	-1.0847353309797896
1116	25	-1.1647233526281582
1201	1	0.2916537646608006
1201	2	0.417936282243641
1201	3	0.3448113710949805
1201	4	0.4662752263019314
1201	5	0.4275432013287599
1201	6	0.30771567649676257
1201	7	0.32769810108771225
1201	8	0.3541331932030545
1201	9	0.6311961680409368
1201	10	0.5944603814338763
1201	11	0.5210633079031268
1201	12	0.4686928016742371
1201	13	0.5902158068880752
1201	14	1.1576806032055131
1201	15	0.9026319943468053
1201	16	0.8168010050636454
1201	17	0.777835353247788
1201	18	0.5785184969900072
1201	19	0.7380166124511192
1201	20	0.8670090486471764
1201	21	0.8381648645240674
1201	22	0.8884277884209268
1201	23	0.5960452575236914
1201	24	0.7262549848665358
1201	25	0.8026912448971117
1202	1	-0.24072619554184627
1202	2	0.015270970297141417
1202	3	0.05076355921258724
1202	4	-0.01468207059505744
1202	5	-0.12289034880279814
1202	6	-0.1876073454486189
1202	7	-0.19246745849758895
1202	8	0.012231967971722213
1202	9	0.16162621845542446
1202	10	0.42701103281176955
1202	11	0.07677548433333925
1202	12	0.06519765720393877
1202	13	0.15303932030931122
1202	14	0.4122654606733178
1202	15	0.39819769969947316
1202	16	0.29083946513988934
1202	17	0.09540169383527797
1202	18	-0.03878728405072707
1202	19	0.08757479283526237
1202	20	0.28405739020655574
1202	21	0.3368820456965125
1202	22	0.25737746375845916
1202	23	0.5159495601358444
1202	24	0.30032913953987617
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2211	9	0.636989708205796
2211	10	0.4844117453171748
2211	11	0.29582580658829727
2211	12	0.40516741923505606
2211	13	0.3087535218929318
2211	14	0.6413300111254827
2211	15	0.48399988589802234
2211	16	0.41842641389173707
2211	17	0.5718721613440536
2211	18	0.43747125072674464
2211	19	0.4047206913939192
2211	20	0.5842952189169779
2211	21	0.575837491934756
2211	22	0.5910429207181949
2211	23	0.7846623233037917
2211	24	0.6240053888031909
2211	25	0.8355347124226924
2212	1	-0.18013934174387813
2212	2	-0.14610559506112608
2212	3	-0.13294271742973152
2212	4	-0.3857123513475784
2212	5	-0.056815357665598754
2212	6	-0.44168826978164333
2212	7	-0.27336660152451486
2212	8	-0.1466476658292919
2212	9	-0.03480825451515557
2212	10	-0.5505699796068413
2212	11	-0.4584322137707417
2212	12	-0.5018081200361948
2212	13	-0.5588030932137329
2212	14	-0.4336658236728539
2212	15	-0.44023135922198264
2212	16	-0.30720324068232685
2212	17	-0.3098091313206126
2212	18	-0.2672562304787576
2212	19	-0.3862974959131833
2212	20	-0.36469229445548024
2212	21	-0.17261259701122356
2212	22	-0.32823726176085444
2212	23	0.22818813392079562
2212	24	0.08774354185464261
2212	25	-0.7113960675843174
2213	1	-0.02761250414241445
2213	2	-1.1861166755132047
2213	3	-0.192866874691056
2213	4	0.15577468586875654
2213	5	0.1665926941927367
2213	6	0.18091064191636264
2213	7	0.11560098758473458
2213	8	0.21616305530388757
2213	9	0.19588081773003552
2213	10	-0.282170422824926
2213	11	0.05159139850589867
2213	12	0.08311687564852832
2213	13	-0.19707938565051109
2213	14	0.19103369390685287
2213	15	0.08236244614593602
2213	16	0.21173134391345627
2213	17	0.2928769404109133
2213	18	0.29221517814644926
2213	19	0.2591914167189344
2213	20	0.34237540638670794
2213	21	-0.26235270091509477
2213	22	-0.8046310482950361
2213	23	0.012280134641557083
2213	24	0.15941798991151757
2213	25	-0.5374778279816681
2214	1	-0.1742128447768323
2214	2	-0.4654056105707518
2214	3	-0.4716747089407143
2214	4	-0.46089131972595965
2214	5	-0.19998996504667588
2214	6	-0.12861788954992193
2214	7	-0.4609828581648401
2214	8	-0.03613106381147446
2214	9	-0.02656804009151159
2214	10	-1.786021366374058
2214	11	-0.8930214008271946
2214	12	-0.46894749816987513
2214	13	-0.23345532541272718
2214	14	-0.8441613775966275
2214	15	-0.5366145188020965
2214	16	-0.761735878144826
2214	17	-0.564092080048006
2214	18	-0.5578602011521241
2214	19	-1.3458029362449295
2214	20	-2.4965583176173496
2214	21	-1.9395997101059308
2214	22	-2.050239507462841
2214	23	-2.6211028417756097
2214	24	-1.6130576584205443
2214	25	-1.928871543170672
2215	1	-0.32733794717593767
2215	2	-0.43026740704399913
2215	3	-0.9779291367437182
2215	4	-0.4738583976361923
2215	5	-0.2610799348495618
2215	6	-0.2908996403691546
2215	7	-0.3071415972351872
2215	8	-0.28774023015002825
2215	9	-0.3723666847698155
2215	10	-0.35060565360706875
2215	11	-0.6739184196136065
2215	12	-0.4916642850695183
2215	13	-0.2772917798269674
2215	14	-0.28996796012211234
2215	15	-0.8245761339665023
2215	16	-0.5257208141084746
2215	17	-0.3256552717074115
2215	18	-0.3628720667442238
2215	19	-2.3219617086086757
2215	20	-2.52990971700023
2215	21	-3.58734100708007
2215	22	-2.6514148170082166
2215	23	-3.7644300473712846
2215	24	-2.5695043731243246
2215	25	-1.9801863082498272
2216	1	-2.3690268677644575
2216	2	-1.7917726706894896
2216	3	-1.368982585780703
2216	4	-1.6600529853707826
2216	5	-2.515889990999206
2216	6	-2.206946685299478
2216	7	-1.7222022144068987
2216	8	-5.9105380685477655
2216	9	-4.935786594928153
2216	10	-2.9759651346707265
2216	11	-1.7334967029111894
2216	12	-1.8491953749842907
2216	13	-2.685524140349983
2216	14	-4.385586714025273
2216	15	-2.927979156258374
2216	16	-3.9158062524256323
2216	17	-3.542763975209276
2216	18	-3.554325835233115
2216	19	-2.532439720059614
2216	20	-1.7873391783464925
2216	21	-1.5052146874015762
2216	22	-1.505500246395897
2216	23	-1.8698095527789178
2216	24	-1.3211489309730808
2216	25	-1.2493019387906301
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	50.0
8	223.0
9	644.0
10	1336.0
11	2506.0
12	4328.0
13	6766.0
14	9209.0
15	11731.0
16	13795.0
17	14679.0
18	15370.0
19	15754.0
20	16459.0
21	17331.0
22	18520.0
23	19670.0
24	21200.0
25	23306.0
26	25460.0
27	28671.0
28	33046.0
29	38933.0
30	46597.0
31	57465.0
32	77431.0
33	111039.0
34	160507.0
35	256320.0
36	446885.0
37	1431019.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29911673104	20.432025481791616	12.48236555692024	23.78649223024814
2	15.66764692210729	20.87920163184504	39.63335438763429	23.819797058413382
3	19.005188714716397	25.387683809706107	29.304855789279387	26.302271686298102
4	12.549427515881067	16.871839852113965	37.493949743906676	33.08478288809829
5	13.094200416614537	38.57085626114772	34.86343807490448	13.471505247333262
6	31.998708118518167	38.09621782898975	16.73007025032767	13.175003802164415
7	26.862272551429246	32.75515727801252	22.428913564416934	17.9536566061413
8	24.45713223380846	37.623441949604484	20.428149668526093	17.491276148060965
9	24.372154759874633	17.264475375398263	21.364765209272633	36.99860465545447
10	14.731954137059125	30.54319309328798	33.39922090002826	21.325631869624637
11	33.11679612878091	25.544749009811323	22.480409813376347	18.858045048031414
12	20.781261907552793	27.440469498039942	31.147783003763596	20.630485590643666
13	26.836525708677257	23.645859070716845	26.329689347004347	23.187925873601554
14	20.41391697670628	23.222402544699868	27.635678425446454	28.7280020531474
15	23.010717338193345	30.749202120297603	23.625953585426526	22.61412695608253
16	21.966687057231418	30.878685252837307	25.479958580953387	21.674669108977888
17	20.642117810451264	30.138213760409432	27.894034294400367	21.325634134738937
18	21.049332868108387	29.608845379800236	28.64714886252842	20.694672889562955
19	21.402916911291804	28.845045836884527	28.874332506563015	20.87770474526065
20	22.29504992247723	28.475752305777007	28.30522613171797	20.923971640027787
21	22.338807921109026	28.563777431364727	27.91047951150744	21.186935136018803
22	21.565841848783123	28.38636401722436	28.54176762714351	21.50602650684901
23	21.657812809404778	28.596824649849985	28.420864710575827	21.324497830169417
24	21.56437047732146	28.427718306008593	28.298815026185615	21.709096190484328
25	22.078431856108246	28.099131092423157	27.71728854801187	22.10514850345672
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3377.0
1	3377.0
2	3553.5
3	3730.0
4	3730.0
5	3730.0
6	5024.5
7	6319.0
8	6319.0
9	6319.0
10	9360.0
11	12401.0
12	12401.0
13	12401.0
14	19779.5
15	27158.0
16	27158.0
17	27158.0
18	41840.5
19	56523.0
20	56523.0
21	56523.0
22	79673.5
23	102824.0
24	102824.0
25	102824.0
26	132968.0
27	163112.0
28	163112.0
29	163112.0
30	194531.5
31	225951.0
32	225951.0
33	225951.0
34	253939.0
35	281927.0
36	281927.0
37	281927.0
38	302549.0
39	323171.0
40	323171.0
41	323171.0
42	327088.5
43	331006.0
44	331006.0
45	331006.0
46	339061.0
47	347116.0
48	347116.0
49	347116.0
50	330889.0
51	314662.0
52	314662.0
53	314662.0
54	288301.0
55	261940.0
56	261940.0
57	261940.0
58	229790.0
59	197640.0
60	197640.0
61	197640.0
62	165910.5
63	134181.0
64	134181.0
65	134181.0
66	104750.0
67	75319.0
68	75319.0
69	75319.0
70	55630.0
71	35941.0
72	35941.0
73	35941.0
74	25429.0
75	14917.0
76	14917.0
77	14917.0
78	9934.0
79	4951.0
80	4951.0
81	4951.0
82	3241.5
83	1532.0
84	1532.0
85	1532.0
86	963.0
87	394.0
88	394.0
89	394.0
90	258.0
91	122.0
92	122.0
93	122.0
94	73.0
95	24.0
96	24.0
97	24.0
98	18.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02966252724048621
2	0.01650576112575442
3	0.02293036379996111
4	0.02580093095226623
5	0.026142665137064452
6	0.009773597685229325
7	0.0032464747555831677
8	0.0026655266414261796
9	0.0031439545001436995
10	0.0018453645979104323
11	0.0014011101576727357
12	0.0021187519457490146
13	0.002050405108789369
14	4.1008102175787384E-4
15	0.0019820582718297233
16	0.002084578527269192
17	0.0020162316903095463
18	0.0023237924566279515
19	1.7086709239911408E-4
20	4.784278587175195E-4
21	0.07917981061774947
22	0.07695853841656099
23	0.07825712831879425
24	8.885088804753932E-4
25	0.22974789243984883
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2926251.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.13669499798442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.17931393759557	56.12469467849171
2	12.226341032308795	16.905776217841503
3	3.481935637966166	7.221885666140373
4	1.2895995840447059	3.5663461244650563
5	0.5941117597400188	2.0537461763930747
6	0.32402942292125736	1.3441394029727924
7	0.19787779261519634	0.9576431616437869
8	0.12692159348861223	0.7019951598144278
9	0.09467337519834905	0.58908638389662
>10	0.44417233138516843	5.443475680959019
>50	0.028309477725163183	1.3512318688662757
>100	0.010531638273183413	1.355309367752891
>500	0.0010912083690663354	0.5094771907085764
>1k	9.920076079614904E-4	1.4026825741434192
>5k	9.920076079614906E-5	0.4725103459104775
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7223	0.2468346016797602	No Hit
CCCATGTACTCTGCGTTGATACCAC	6556	0.2240409315537184	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4148	0.14175133985430505	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3697	0.12633912811990494	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3060	0.10457066054825781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.4173418479822816E-5	0.0	0.0	0.0	0.0
9	3.4173418479822816E-5	0.0	0.0	0.0	0.0
10	3.4173418479822816E-5	0.0	0.0	1.0252025543946845E-4	0.0
11	3.4173418479822816E-5	0.0	0.0	1.0252025543946845E-4	0.0
12	3.4173418479822816E-5	0.0	0.0	1.0252025543946845E-4	0.0
13	3.4173418479822816E-5	0.0	0.0	1.3669367391929126E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCC	60	4.1068878E-4	12.661928	11
TACCGTA	75	2.0822328E-4	11.395734	7
TACACCG	110	4.990816E-7	11.224223	5
GTCGTAC	60	0.0058702044	11.085248	1
TAGCACG	70	0.0014939771	10.855493	4
AGACCGT	80	3.7870833E-4	10.683136	6
ACCGTAT	75	0.0026570018	10.129541	8
ACCGCCC	85	6.6193833E-4	10.055059	8
CGCCCTA	180	3.9108272E-10	10.03277	16
TCTACAC	390	0.0	9.986174	3
ACACCGT	105	4.126047E-5	9.948317	6
CTAGGAC	250	0.0	9.8790045	3
GTCTTAC	295	0.0	9.662686	1
GTACGGA	120	1.6979904E-5	9.501641	1
TTAGACT	210	6.730261E-11	9.498556	4
TAGCGTG	80	0.004527596	9.497419	5
CCGTTCA	80	0.0045307884	9.496608	9
AACCGTG	80	0.0045314273	9.496446	7
CTTAGAC	245	1.8189894E-12	9.305185	3
CCCGTAC	235	7.2759576E-12	9.302501	16
>>END_MODULE