FastQCFastQC Report
Wed 25 May 2016
SRR1294759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926251
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT121910.41660814468751994No Hit
GGTATCAACGCAGAGTACTTTTTTT101240.3459716886897262No Hit
TATCAACGCAGAGTACTTTTTTTTT82950.28346850629013026No Hit
GTACATGGGAAGCAGTGGTATCAAC75480.25794096268570266No Hit
CCCATGTACTCTGCGTTGATACCAC69590.23781281920108702No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54400.18590339653023613No Hit
GAGTACATGGGAAGCAGTGGTATCA43850.14985044003402304No Hit
CATGTACTCTGCGTTGATACCACTG30350.10371632508626226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGG550.003083237212.08373312
GTTCGGT600.005795039211.1044571
GTCTTAG3450.010.7595981
AGCGCAA909.576558E-510.54947110
TACGCTG750.002617275310.1479535
TGTACCG856.5097923E-410.0733365
ACACCGT1356.484024E-79.8478566
CTGTTCG800.00453899859.4945249
TAGACTG4150.09.3990835
GTTCTAG2850.09.3511211
GGCTTAG2357.2759576E-129.3155931
CTAGACT3500.09.2418864
TAGACAG5200.09.1477945
TAGGACC1151.0938666E-49.1000664
ACTAGAC2106.511982E-109.0606723
CCTAGAC2850.09.0129843
TAATACT6150.08.9722764
AACAGCG850.00747224748.9361757
CAGTGCG850.00747329468.9360229
GTAGCAC2502.5465852E-118.7526093
Produced by FastQC (version 0.11.5)