FastQCFastQC Report
Wed 25 May 2016
SRR1294758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3649744
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1208413.3109445484395614No Hit
CCCATGTACTCTGCGTTGATACCAC1138723.1199996492904707No Hit
GAGTACATGGGAAGCAGTGGTATCA582681.5964955350293062No Hit
CATGTACTCTGCGTTGATACCACTG464661.2731303894190935No Hit
GCGTTGATACCACTGCTTCCCATGT404821.1091736845104754No Hit
GCTTCCCATGTACTCTGCGTTGATA335200.9184205796351744No Hit
GTATCAACGCAGAGTACATGGGAAG309280.8474018999688745No Hit
ACGCAGAGTACATGGGAAGCAGTGG304260.8336475106199229No Hit
ACTCTGCGTTGATACCACTGCTTCC240510.6589777255610256No Hit
TATCAACGCAGAGTACATGGGAAGC228720.6266740900183685No Hit
GGTATCAACGCAGAGTACATGGGAA197220.5403666668127957No Hit
CAGTGGTATCAACGCAGAGTACATG166390.45589498879921436No Hit
GTGGTATCAACGCAGAGTACATGGG148460.406768255526963No Hit
GCAGTGGTATCAACGCAGAGTACAT146020.4000828551262774No Hit
ATACCACTGCTTCCCATGTACTCTG125960.345120096094411No Hit
GTACTCTGCGTTGATACCACTGCTT117350.3215294004182211No Hit
CTGCTTCCCATGTACTCTGCGTTGA114160.31278906136978374No Hit
CATGGGAAGCAGTGGTATCAACGCA113800.3118026908188629No Hit
GGGAAGCAGTGGTATCAACGCAGAG109990.3013636024882841No Hit
ACATGGGAAGCAGTGGTATCAACGC107470.29445900863183827No Hit
GTACATGGTAAGCAGTGGTATCAAC105560.2892257648755639No Hit
GCAGAGTACATGGGAAGCAGTGGTA102750.2815265947419874No Hit
TACCACTGCTTCCCATGTACTCTGC98140.26889557185380675No Hit
GTTGATACCACTGCTTCCCATGTAC95790.2624567640908513No Hit
AAGCAGTGGTATCAACGCAGAGTAC94720.25952505162005884No Hit
CCACTGCTTCCCATGTACTCTGCGT91410.2504559223879812No Hit
CTTCCCATGTACTCTGCGTTGATAC89420.24500348517594658No Hit
GATACCACTGCTTCCCATGTACTCT89390.24492128763003654No Hit
CCATGTACTCTGCGTTGATACCACT80150.21960444348973515No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA77810.21319303490874977No Hit
ATGGGAAGCAGTGGTATCAACGCAG77300.21179567662827858No Hit
ACCATGTACTCTGCGTTGATACCAC71860.19689052163658602No Hit
GTACATGGGTAAGCAGTGGTATCAA70100.19206826560986195No Hit
TTCCCATGTACTCTGCGTTGATACC66370.18184837073504334No Hit
TGATACCACTGCTTCCCATGTACTC61600.1687789609353423No Hit
CCCCATGTACTCTGCGTTGATACCA54170.14842136873161516No Hit
GGTAAGCAGTGGTATCAACGCAGAG47680.13063929963307014No Hit
ATCAACGCAGAGTACATGGGAAGCA45280.1240634959602646No Hit
GCTTACCATGTACTCTGCGTTGATA39470.10814457123568119No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA37350.10233594465803629No Hit
GAGTACATGGTAAGCAGTGGTATCA37270.10211675120227612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG907.4879736E-611.6083485
CCTATAC2850.011.3317223
CGTGTGA600.005898272611.07826610
GACCGTG951.36839335E-510.9949727
GTACAAA8300.010.7608991
CGTTATA1002.4031184E-510.4490882
ACAGCGC750.002659100310.1287018
CGTTGAC750.002659402310.12856314
CGCAAAA3100.010.1120212
GATACGA1252.552506E-69.88604718
CTAGTAC1201.7046985E-59.498653
TATTCCG1002.763722E-49.4977395
GCTACAC2550.09.3124023
ACTGTCC3950.09.1350638
GTTCTAG2501.8189894E-129.1215781
CCTAGAC2304.1836756E-119.0856653
GTCTTGC4400.09.0697521
GTTCTAC2106.657501E-109.0491861
TAGGACT3150.09.0459614
TATAACG950.00182613398.9992152
Produced by FastQC (version 0.11.5)