FastQCFastQC Report
Wed 25 May 2016
SRR1294758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3649744
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1247843.418979522947363No Hit
GTACATGGGAAGCAGTGGTATCAAC1204083.299080702646542No Hit
GAGTACATGGGAAGCAGTGGTATCA651501.7850567053470052No Hit
CATGTACTCTGCGTTGATACCACTG529231.4500469073995328No Hit
GCGTTGATACCACTGCTTCCCATGT414901.1367920599362586No Hit
ACGCAGAGTACATGGGAAGCAGTGG375621.029168073158008No Hit
GCTTCCCATGTACTCTGCGTTGATA362530.9933025439592476No Hit
GTATCAACGCAGAGTACATGGGAAG334780.9172698139924335No Hit
ACTCTGCGTTGATACCACTGCTTCC276420.7573681880153786No Hit
TATCAACGCAGAGTACATGGGAAGC271500.7438877904861273No Hit
GGTATCAACGCAGAGTACATGGGAA219480.601357245878067No Hit
CAGTGGTATCAACGCAGAGTACATG201940.5532990807026466No Hit
GTGGTATCAACGCAGAGTACATGGG165250.4527714820546318No Hit
GCAGTGGTATCAACGCAGAGTACAT157770.4322768939410545No Hit
CATGGGAAGCAGTGGTATCAACGCA149040.40835740808122434No Hit
ATACCACTGCTTCCCATGTACTCTG143440.3930138661780114No Hit
ACATGGGAAGCAGTGGTATCAACGC136180.3731220600677746No Hit
GGGAAGCAGTGGTATCAACGCAGAG130760.35827170344002207No Hit
CTGCTTCCCATGTACTCTGCGTTGA126740.3472572322880728No Hit
AAGCAGTGGTATCAACGCAGAGTAC117060.32073482414109045No Hit
GTACTCTGCGTTGATACCACTGCTT116520.3192552683147092No Hit
TACCACTGCTTCCCATGTACTCTGC109310.2995004581143225No Hit
GCAGAGTACATGGGAAGCAGTGGTA108750.29796610392400125No Hit
CCACTGCTTCCCATGTACTCTGCGT104330.28585566549325103No Hit
GATACCACTGCTTCCCATGTACTCT103900.28467750066854003No Hit
GTTGATACCACTGCTTCCCATGTAC103810.28443090803080984No Hit
CTTCCCATGTACTCTGCGTTGATAC101570.27829349126952463No Hit
GTACATGGTAAGCAGTGGTATCAAC99430.2724300663279397No Hit
GTATCAACGCAGAGTACTTTTTTTT98420.26966274894896736No Hit
CCATGTACTCTGCGTTGATACCACT95970.2629499493663117No Hit
ATGGGAAGCAGTGGTATCAACGCAG93710.2567577342410865No Hit
ACCATGTACTCTGCGTTGATACCAC79530.21790569420759373No Hit
GTACATGGGTAAGCAGTGGTATCAA73450.20124699156981968No Hit
TATCAACGCAGAGTACTTTTTTTTT72910.19976743574343842No Hit
GGTATCAACGCAGAGTACTTTTTTT72720.19924685128600803No Hit
TTCCCATGTACTCTGCGTTGATACC69660.19086270160318095No Hit
TGATACCACTGCTTCCCATGTACTC67230.18420470038446532No Hit
CCCCATGTACTCTGCGTTGATACCA62520.17129968567658443No Hit
GGTAAGCAGTGGTATCAACGCAGAG56860.15579174868155138No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55870.15307922966651907No Hit
ATCAACGCAGAGTACATGGGAAGCA54940.15053110574330691No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53150.14562665217067278No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47130.12913234462471887No Hit
GCTTACCATGTACTCTGCGTTGATA43350.11877545384005016No Hit
GAGTACATGGTAAGCAGTGGTATCA42570.11663831764638835No Hit
GAGTACTTTTTTTTTTTTTTTTTTT41440.11354221008377575No Hit
GGAAGCAGTGGTATCAACGCAGAGT41260.11304902480831533No Hit
AAAAAGTACTCTGCGTTGATACCAC40140.10998031642767273No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG400.005302581514.2412057
TATTCCG655.3776654E-513.1742435
CAGCGTA655.4888216E-513.1455479
AGTGCGG600.005905909511.07634110
TACGCAG803.719655E-410.7040735
AGCGTCA1201.4953202E-610.3076263
CGCAAAA1409.48221E-810.1944952
TCTACGC1054.0389736E-59.9678143
ACGCTGA1151.0015538E-59.934281
GTCCTAG2400.09.9170331
TAGCACC2400.09.9111794
CTTGGAC2900.09.5147313
CTGCGTG900.00112072059.4940079
GCTACAC2304.1836756E-119.10104753
AACTGTC2304.3655746E-119.0813477
TTAGACT2750.08.9957464
ACAGTCC2850.08.9944458
TAAGACT2651.8189894E-128.9761624
TAGACAG3400.08.9550425
ATGCACG850.0073448128.9550424
Produced by FastQC (version 0.11.5)