FastQCFastQC Report
Wed 25 May 2016
SRR1294757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589542
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC53320.20590513689293322No Hit
CCCATGTACTCTGCGTTGATACCAC47400.18304395140144475No Hit
GTATCAACGCAGAGTACTTTTTTTT35370.1365878599381667No Hit
GGTATCAACGCAGAGTACTTTTTTT26120.10086725760771596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG400.005295121614.2440049
AGGACCG604.1015327E-412.6637825
GTCGTAC658.0120174E-411.694841
ACCGAAC600.00589504111.0788848
TGCGGGA700.001493616510.855722
CAATCCG700.001497230310.85257410
AACACCG803.7804485E-410.6850675
ACCGCCC803.7865248E-410.6832098
GAATCGT750.002657000410.1294616
GTTCTAG2106.730261E-119.5020581
GTGTAGG3700.09.5020581
TAGCACC2002.6557245E-109.4978374
TGTACCG800.00452565639.4978375
ACACCGT1106.8680674E-59.496376
TATCGGT1901.0604708E-99.49600313
CTAGAAC2650.09.3189933
CTAGCAC2151.07320375E-109.2773163
TCGGTCA1951.6698323E-99.26056215
ATCGGTC1951.6952981E-99.25269514
GGACTGT4450.09.1762686
Produced by FastQC (version 0.11.5)