##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294757_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2589542
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.161093351642876	33.0	33.0	33.0	27.0	33.0
2	31.233057814856835	33.0	33.0	33.0	27.0	33.0
3	31.061720180634257	33.0	33.0	33.0	27.0	33.0
4	34.484844810395046	37.0	37.0	37.0	27.0	37.0
5	34.79707260975107	37.0	37.0	37.0	27.0	37.0
6	34.99509334083015	37.0	37.0	37.0	33.0	37.0
7	34.96041037372632	37.0	37.0	37.0	33.0	37.0
8	34.87768145872899	37.0	37.0	37.0	33.0	37.0
9	34.564974037880056	37.0	37.0	37.0	27.0	37.0
10	34.665944788692364	37.0	37.0	37.0	27.0	37.0
11	34.91204081648415	37.0	37.0	37.0	33.0	37.0
12	34.83889931115232	37.0	37.0	37.0	33.0	37.0
13	34.77292277939497	37.0	37.0	37.0	27.0	37.0
14	35.9599322969081	40.0	37.0	40.0	27.0	40.0
15	36.2845734882848	40.0	37.0	40.0	27.0	40.0
16	36.29867134806078	40.0	37.0	40.0	27.0	40.0
17	36.240181854551885	40.0	37.0	40.0	27.0	40.0
18	36.2433557748822	40.0	37.0	40.0	27.0	40.0
19	36.33080676042327	40.0	37.0	40.0	27.0	40.0
20	36.2366974546078	40.0	37.0	40.0	27.0	40.0
21	36.179302749289256	40.0	37.0	40.0	27.0	40.0
22	36.038221430662254	40.0	37.0	40.0	27.0	40.0
23	35.65426511715199	37.0	37.0	40.0	27.0	40.0
24	35.97087979264287	37.0	37.0	40.0	27.0	40.0
25	34.87250757083685	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2528691692014142
1101	2	0.3897837557697059
1101	3	0.22962409477470302
1101	4	0.2883602274332304
1101	5	0.33350771308905536
1101	6	0.21042376255174844
1101	7	0.23204347901159394
1101	8	0.30207700425588513
1101	9	0.5374170128291951
1101	10	0.4885357628278939
1101	11	0.2750539783897352
1101	12	0.2956418809423198
1101	13	0.32489108804378475
1101	14	0.9457673908548969
1101	15	0.7228232616123762
1101	16	0.7015409322375419
1101	17	0.7732918363215617
1101	18	0.7057725076245873
1101	19	0.6011444350130688
1101	20	0.7254025822988481
1101	21	0.6554410460960298
1101	22	0.6822476546518814
1101	23	1.0258478987023665
1101	24	0.7931210724637978
1101	25	0.5195609406711128
1102	1	0.14382524155283605
1102	2	0.34843421383876816
1102	3	0.13213172925908268
1102	4	0.0894701640082829
1102	5	0.08286171825701416
1102	6	0.007041098703965076
1102	7	0.1354555062608327
1102	8	0.12533281820937248
1102	9	0.3639674004260982
1102	10	0.4633713277797398
1102	11	0.16207535494599767
1102	12	0.20294749522468436
1102	13	0.22126764430406354
1102	14	0.726951323201618
1102	15	0.57013944671926
1102	16	0.34727736634607
1102	17	0.5260108872964224
1102	18	0.5859111028794644
1102	19	0.4754162226569534
1102	20	0.6149550840606466
1102	21	0.6147081355956772
1102	22	0.6496895151169966
1102	23	1.0466959137364285
1102	24	0.7816282371337522
1102	25	0.6495879550004489
1103	1	0.1655306679094224
1103	2	0.4153592784382525
1103	3	0.19440563106786968
1103	4	-0.09166678689611274
1103	5	0.16761624022084476
1103	6	0.03753205477632093
1103	7	0.12434439514971984
1103	8	0.3224904409483855
1103	9	0.5285668836302335
1103	10	0.5290044027151382
1103	11	0.33261799060490915
1103	12	0.31250821873889123
1103	13	0.31945885877434677
1103	14	0.9452975764315923
1103	15	0.6396484906469055
1103	16	0.37208356789645336
1103	17	0.39345274776154326
1103	18	0.39805580572183885
1103	19	0.549059633509664
1103	20	0.6283917765671134
1103	21	0.587059556784304
1103	22	0.5039530810084685
1103	23	1.0660757586976644
1103	24	0.7247806919141127
1103	25	0.4945491953105261
1104	1	0.2260834014963038
1104	2	0.43817600869061835
1104	3	0.2004767526558311
1104	4	0.10650009681950223
1104	5	0.09826920189192379
1104	6	0.11331304873418446
1104	7	0.10878248434786286
1104	8	0.49529410837754995
1104	9	0.619207985720351
1104	10	0.6207122023174705
1104	11	0.42647281311338503
1104	12	0.38824660063734484
1104	13	0.40886108670033394
1104	14	0.832467025512841
1104	15	0.7297027960936049
1104	16	0.7010263966059469
1104	17	0.6103466011880201
1104	18	0.544660169701956
1104	19	0.714936015991789
1104	20	0.7781111419199789
1104	21	0.7552114631255264
1104	22	0.8184320737950017
1104	23	1.0945271194582773
1104	24	0.8804331051460537
1104	25	0.6760637382546406
1105	1	0.07761830126970537
1105	2	0.16205004110846843
1105	3	0.11263814164950148
1105	4	0.06257260372863982
1105	5	0.2021527884683323
1105	6	0.08250003245581183
1105	7	0.17657367179403138
1105	8	0.22505103764152778
1105	9	-1.794834378239429
1105	10	-0.307837514819461
1105	11	-0.02048595511566731
1105	12	-0.01607962863194956
1105	13	-0.016095824886342314
1105	14	-1.8096801506177513
1105	15	-0.5432318536723031
1105	16	-0.3278557957788166
1105	17	-0.08303304035215575
1105	18	-0.22657205369699795
1105	19	0.029210946900384727
1105	20	0.12144911554398163
1105	21	0.4060549753731024
1105	22	0.40917612756765465
1105	23	0.7254165269163053
1105	24	0.4278493655242315
1105	25	0.0955352291400473
1106	1	0.14446430773368135
1106	2	0.004716462634405616
1106	3	0.20783580249085176
1106	4	-1.214403359577659
1106	5	-0.27711024108402427
1106	6	-0.09319732841069595
1106	7	-0.055523797450483414
1106	8	0.07692233230713441
1106	9	0.4537810604657153
1106	10	0.33150483066123826
1106	11	0.24751092256555296
1106	12	0.14035079978373233
1106	13	0.03339115362513212
1106	14	0.7132937289923404
1106	15	0.3688698732796354
1106	16	0.1710467156234543
1106	17	0.05051059792334911
1106	18	0.20175815086648896
1106	19	0.17397057996341658
1106	20	0.36587700223735453
1106	21	0.5173956371240536
1106	22	0.4948439425251081
1106	23	0.8629154786171327
1106	24	0.5795406752853509
1106	25	0.48588797374856796
1107	1	0.2510059231208359
1107	2	0.20798161189904363
1107	3	0.2188989741345786
1107	4	0.39523718872410285
1107	5	0.25406251546678504
1107	6	0.0909149819729933
1107	7	0.10849637484867714
1107	8	0.2257152008005363
1107	9	0.4035509510222681
1107	10	0.378352614207472
1107	11	0.13090885629592464
1107	12	0.15598975429467998
1107	13	0.10723883814885937
1107	14	0.6089480555685682
1107	15	0.37712198458093127
1107	16	0.3451881669161665
1107	17	0.518611204789849
1107	18	0.38618804057427525
1107	19	0.3157564272895712
1107	20	0.5427403936167394
1107	21	0.5113118829450727
1107	22	0.6145937456589365
1107	23	0.5773572038721113
1107	24	0.6009964947437467
1107	25	0.5843198816293409
1108	1	0.47009273889752023
1108	2	0.4152606750553005
1108	3	0.5175767919562517
1108	4	0.7119897816427567
1108	5	0.49728571280370915
1108	6	0.33660023695478714
1108	7	0.30594994991264457
1108	8	0.5134252707703624
1108	9	0.2075275849507534
1108	10	0.5109457162643807
1108	11	0.3717635336368872
1108	12	0.40713100531488067
1108	13	0.3400698192854321
1108	14	-1.0124408868140762
1108	15	0.03463113201276968
1108	16	0.3974918524829434
1108	17	0.49950274696890773
1108	18	0.5397878645034098
1108	19	0.6521864070230805
1108	20	0.7141894018425461
1108	21	0.7378545663751481
1108	22	0.6661597676494537
1108	23	0.9378193899019891
1108	24	0.8422154186939679
1108	25	0.8464963743343219
1109	1	0.42463779898164944
1109	2	0.30916927727063737
1109	3	0.4556284269436546
1109	4	0.5679813807122613
1109	5	0.5665170336903316
1109	6	0.3594329294710903
1109	7	0.22486144484436466
1109	8	0.4120395390939109
1109	9	0.46350327408016767
1109	10	0.47375737632199844
1109	11	0.28258283528693795
1109	12	0.43236272394999986
1109	13	0.41284128263833253
1109	14	0.7571747434967762
1109	15	0.49582986162815956
1109	16	0.5502639184850864
1109	17	0.4020684658691991
1109	18	0.5551102215851387
1109	19	0.5087675778500369
1109	20	0.5967806730708674
1109	21	0.6231237095284499
1109	22	0.6973936317158902
1109	23	1.1167990444634412
1109	24	0.8369271715595659
1109	25	0.7667308048910542
1110	1	0.444663550686748
1110	2	0.272838289464687
1110	3	0.4329516037158072
1110	4	0.6266316611540361
1110	5	0.5187612211456383
1110	6	0.39307073791546543
1110	7	0.33565922506638657
1110	8	0.40117714204728117
1110	9	0.467827434358405
1110	10	0.4994870475601445
1110	11	0.35093549823655223
1110	12	0.34549348037070615
1110	13	0.266428229089513
1110	14	0.7852623943188561
1110	15	0.6916989579191082
1110	16	0.5012990942689797
1110	17	0.5203123848796096
1110	18	0.5881910662600731
1110	19	0.47395894580246534
1110	20	0.5982926354980833
1110	21	0.6173421072687617
1110	22	0.8224630591680651
1110	23	0.883622759313198
1110	24	0.8210968809976578
1110	25	0.7645394155580547
1111	1	0.20836003178681395
1111	2	0.2116343843589661
1111	3	0.3501074002149309
1111	4	0.600039050803062
1111	5	0.3977837314424164
1111	6	0.31391126776576783
1111	7	0.24055839799920165
1111	8	0.49234725633409937
1111	9	0.5753002625851948
1111	10	0.2168144521884301
1111	11	0.24724271528584296
1111	12	0.21185904535051492
1111	13	0.28394316605473335
1111	14	-1.677187157149291
1111	15	-0.35362157071792666
1111	16	0.10675077304796332
1111	17	0.2932332404003759
1111	18	0.35222738045344215
1111	19	0.3248913869135208
1111	20	0.3468692854764157
1111	21	0.4706948529696646
1111	22	0.3283172762540971
1111	23	-1.6651043831781323
1111	24	-0.4029288403893645
1111	25	0.12967560836579395
1112	1	-0.024412970622154972
1112	2	-0.16598579722749918
1112	3	0.1577396491032914
1112	4	0.33989236612123364
1112	5	-0.5646807872468145
1112	6	-0.8856579414177261
1112	7	-0.9563296107831292
1112	8	-0.9064786259383695
1112	9	-0.8095777464239973
1112	10	-0.9200939186544161
1112	11	-0.9950389156643098
1112	12	-0.9679597597182834
1112	13	-0.8980216472636755
1112	14	-0.728421830184601
1112	15	-0.9145325408845508
1112	16	-0.9005120637042126
1112	17	-1.0577684585108216
1112	18	-1.0729885130088306
1112	19	-0.9813643327906547
1112	20	-1.1183823204742964
1112	21	-1.8508315484420663
1112	22	-1.7573310870137249
1112	23	-1.4538243981669652
1112	24	-1.7430597762000488
1112	25	-2.097737296380828
1113	1	0.1631396076015541
1113	2	-0.023122635249311685
1113	3	-0.012682013783397394
1113	4	0.27699633860184747
1113	5	0.12948751768912103
1113	6	0.026738834219770524
1113	7	0.15926958811502345
1113	8	0.146048655157621
1113	9	0.32811538153410424
1113	10	0.014588982523811467
1113	11	-0.06481747008336214
1113	12	0.05132505613827476
1113	13	-0.05977451337730599
1113	14	0.05578753273167081
1113	15	-0.03519909036704405
1113	16	0.1285769962451866
1113	17	0.12049374891621767
1113	18	0.1697555852165138
1113	19	-0.053317541072175345
1113	20	-0.5496514962923413
1113	21	-0.3954458478488334
1113	22	0.04052160336356536
1113	23	-0.01785111228048919
1113	24	0.08580901324092594
1113	25	-0.9951400668266785
1114	1	-0.7343675574330746
1114	2	-0.5287889834699939
1114	3	-0.2916317391237371
1114	4	-1.2735947767552105
1114	5	-0.3963573593111178
1114	6	-0.38972283284496
1114	7	-0.31466759841470804
1114	8	-0.21031329850848834
1114	9	-0.1967920701019139
1114	10	-0.25681357300521057
1114	11	-0.3400503337088736
1114	12	-0.4074670384401813
1114	13	-0.3915096459304479
1114	14	-0.39006271804453974
1114	15	-1.062554595501986
1114	16	-0.5758175583284952
1114	17	-0.678237442867875
1114	18	-0.5962015439929473
1114	19	-0.560294493544113
1114	20	-1.7516457642646373
1114	21	-6.591833476222373
1114	22	-7.683636505067295
1114	23	-8.074275759999619
1114	24	-5.876332735027528
1114	25	-6.921122273657343
1115	1	-0.8750662098558557
1115	2	-0.8982811533273996
1115	3	-0.5689057756797631
1115	4	-0.3504884822238665
1115	5	-0.5202430963768663
1115	6	-0.6580683638106564
1115	7	-0.788850940811777
1115	8	-0.5570020791446666
1115	9	-2.245031209754046
1115	10	-1.2146537394706272
1115	11	-0.9922869247625101
1115	12	-0.7899156977759816
1115	13	-1.0556460719934364
1115	14	-0.776536480845138
1115	15	-1.6725845749472867
1115	16	-0.9809212074791134
1115	17	-2.7433489498131536
1115	18	-2.4050468629594945
1115	19	-1.5842548911807839
1115	20	-1.3269538543366295
1115	21	-0.8256125454539216
1115	22	-0.5389779619623383
1115	23	0.06570589746505817
1115	24	-0.9986784826010577
1115	25	-0.5164491901757629
1116	1	-1.4037150837097983
1116	2	-0.771142159127713
1116	3	-0.44357660611357375
1116	4	-0.032927251761392995
1116	5	-0.1950509685920494
1116	6	-0.1648497491901324
1116	7	-0.5314895123315182
1116	8	-1.4388787543535102
1116	9	-0.6551338416158359
1116	10	-2.1607776469740685
1116	11	-0.865067973706303
1116	12	-0.6236260779079572
1116	13	-0.6661623441627853
1116	14	-0.8893729254176037
1116	15	-0.8593346086976226
1116	16	-0.6367300731048573
1116	17	-0.40657197511012555
1116	18	-1.1438679097869269
1116	19	-0.5986583952366331
1116	20	-0.8913797439396944
1116	21	-0.31541448733786126
1116	22	-0.04469701254940617
1116	23	0.1738869311767175
1116	24	0.038585090075336836
1116	25	0.1453171730423719
1201	1	0.17912797707633388
1201	2	0.30941242978417094
1201	3	0.14615410134538465
1201	4	0.21701726534799093
1201	5	0.300084662918195
1201	6	0.22313569452457216
1201	7	0.240128510229205
1201	8	0.3344204282132992
1201	9	0.6353205117858636
1201	10	0.5183916064731662
1201	11	0.3734908984721059
1201	12	0.41233416135371925
1201	13	0.3707453041394828
1201	14	1.0279765144799313
1201	15	0.6701995939624013
1201	16	0.7280688906250603
1201	17	0.6075174581307223
1201	18	0.5414616181632468
1201	19	0.5145757741399635
1201	20	0.7085267214433344
1201	21	0.6213108916162824
1201	22	0.7599081621064414
1201	23	0.5894175932893901
1201	24	0.6534195161195129
1201	25	0.47230983616428546
1202	1	-0.36993346071234967
1202	2	0.011451302653597395
1202	3	-0.17826913907581954
1202	4	-0.3750687875215135
1202	5	-0.12035730797315125
1202	6	-0.17825332026226448
1202	7	-0.07882648697221128
1202	8	-0.05321747497418983
1202	9	0.14325612646871377
1202	10	0.17950589826264718
1202	11	-9.635627690940396E-4
1202	12	0.0066480294970574505
1202	13	0.19040117757941033
1202	14	0.4159055648716503
1202	15	0.2712438149768275
1202	16	0.2268065450906107
1202	17	0.05996600948805053
1202	18	-0.048118344708740324
1202	19	-0.015112378224372947
1202	20	0.11886399728273744
1202	21	0.12752765947120537
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2210	25	0.7184920437677391
2211	1	0.3299889835968166
2211	2	0.31388008837153336
2211	3	0.29724862210066405
2211	4	0.6276903485080894
2211	5	0.39753577473629775
2211	6	0.2960153018747107
2211	7	0.2616881135021103
2211	8	0.3792023425975941
2211	9	0.5336593483536944
2211	10	0.5763593530916893
2211	11	0.3436094162127006
2211	12	0.3903946341273823
2211	13	0.337779333588216
2211	14	0.6093244105352085
2211	15	0.5134778743586352
2211	16	0.3347950890071374
2211	17	0.5077824782543416
2211	18	0.5218142987892875
2211	19	0.4386629527876238
2211	20	0.5486253781187216
2211	21	0.5733135232846394
2211	22	0.598869209090374
2211	23	0.7496407908899982
2211	24	0.6849751348241284
2211	25	0.693109900411585
2212	1	-0.1022352238302453
2212	2	-0.1359214388327885
2212	3	-0.018819887495560295
2212	4	-0.20255538497060144
2212	5	0.014313562460536389
2212	6	-0.41628915306392855
2212	7	-0.28746520412671117
2212	8	-0.07445827566631635
2212	9	0.015558408658279177
2212	10	-0.2696096932456129
2212	11	-0.24017111959640403
2212	12	-0.3260082809762963
2212	13	-0.4026246263027389
2212	14	-0.19891101486611973
2212	15	-0.4142376835295707
2212	16	-0.3389856552689139
2212	17	-0.36147048657483793
2212	18	-0.01201731142166551
2212	19	-0.1834095274907952
2212	20	-0.15620786671834708
2212	21	-0.27823507955762494
2212	22	-0.25218103512252554
2212	23	0.2217495076287932
2212	24	-0.050340269534302706
2212	25	-0.6232924015540107
2213	1	-0.06571232845362118
2213	2	-1.243532416392533
2213	3	-0.16036461972590033
2213	4	0.2098780965077296
2213	5	0.08422520260267419
2213	6	0.08786730201330784
2213	7	0.10111424748777864
2213	8	0.16979778372141396
2213	9	0.16382506917298656
2213	10	-0.24184084390562077
2213	11	-0.02176212059175242
2213	12	0.10347829088570393
2213	13	-0.30671549372016216
2213	14	0.13601457535408912
2213	15	-0.02173217635360203
2213	16	0.15442354395321445
2213	17	0.23999698202957376
2213	18	0.24166301453956152
2213	19	0.29125094849129596
2213	20	0.269221880674138
2213	21	-0.23043223030153825
2213	22	-0.7857170443255939
2213	23	0.08326529379078806
2213	24	0.25536916792869846
2213	25	-0.36616725917547654
2214	1	-0.11882016411009744
2214	2	-0.3884155862256051
2214	3	-0.3652362850409965
2214	4	-0.3165022650595759
2214	5	-0.17290495622886937
2214	6	-0.10583060972278702
2214	7	-0.35476083154400584
2214	8	0.017407402654214366
2214	9	0.06110447498326721
2214	10	-1.9886232096770513
2214	11	-0.7372336442484411
2214	12	-0.32301122293245754
2214	13	-0.14301525142573723
2214	14	-0.6305870409812613
2214	15	-0.35095262550123607
2214	16	-0.588630308672613
2214	17	-0.39795211142885734
2214	18	-0.22139877643179062
2214	19	-1.2999383244011113
2214	20	-2.3093770545686567
2214	21	-1.8287572698126766
2214	22	-1.904537500468706
2214	23	-2.3819373412228657
2214	24	-1.4750616507968246
2214	25	-1.6884621862001055
2215	1	-0.059996020184762244
2215	2	-0.16523330759029164
2215	3	-0.5662952371713388
2215	4	-0.04359695607013947
2215	5	0.0029749727355934397
2215	6	0.012168565376541096
2215	7	-0.08161401872143159
2215	8	0.03620805428440832
2215	9	-0.008340698032192506
2215	10	-0.005968302118610325
2215	11	-0.37398294120301756
2215	12	-0.22557529197763415
2215	13	-0.055131375273013816
2215	14	0.03975558999825779
2215	15	-0.4429549716023189
2215	16	-0.2817884318967785
2215	17	-0.022026451481785614
2215	18	-0.11724850261292374
2215	19	-1.9929527073146858
2215	20	-2.2535049221054564
2215	21	-3.1848395603304454
2215	22	-2.103376829583013
2215	23	-3.2376064272294585
2215	24	-2.218736867637716
2215	25	-1.490895905744047
2216	1	-1.1543367643171116
2216	2	-0.9177902738908585
2216	3	-0.5819633840070821
2216	4	-0.5442676102595527
2216	5	-1.5341202601592983
2216	6	-1.1975217019344484
2216	7	-0.8245681060698615
2216	8	-5.117651503031233
2216	9	-3.946298808966432
2216	10	-1.830967910477149
2216	11	-0.8903628575122084
2216	12	-1.1646353151458442
2216	13	-1.7759955834794567
2216	14	-3.063337676262684
2216	15	-1.7317875231891335
2216	16	-2.7527454605432737
2216	17	-2.334952616066552
2216	18	-2.19150310145924
2216	19	-1.3981420782615857
2216	20	-0.6619671149051456
2216	21	-0.5292212924987041
2216	22	-0.5098130059656611
2216	23	-0.7743234786878688
2216	24	-0.24560307595851327
2216	25	0.06818039500469553
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	77.0
8	333.0
9	905.0
10	1621.0
11	2803.0
12	4463.0
13	6493.0
14	8542.0
15	10698.0
16	12255.0
17	13347.0
18	13682.0
19	14244.0
20	14320.0
21	15493.0
22	16416.0
23	17259.0
24	18290.0
25	19909.0
26	21599.0
27	24449.0
28	27651.0
29	32818.0
30	39680.0
31	48588.0
32	66111.0
33	93928.0
34	138215.0
35	221516.0
36	390473.0
37	1293363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.183840912251426	20.381302245090314	12.506257822277847	23.928599020380414
2	15.79823487767697	20.60240177446895	39.72123874494095	23.878124602913136
3	18.989639697228718	25.385233082671917	28.969527816928203	26.655599403171166
4	12.645749298267367	16.78754583423621	37.564934651088144	33.00177021640828
5	13.19036414115742	38.17518451167943	35.18761713034706	13.446834216816095
6	31.97261361687797	38.41678651813838	16.616434610530348	12.9941652544533
7	26.947927193919774	32.55729174913082	22.3284414149555	18.166339641993908
8	24.72498573084709	37.3245512071433	20.311086842180465	17.639376219829142
9	24.293609895949185	17.101576209189094	21.450826104149677	37.153987790712044
10	14.628408397994534	30.379494601901584	33.56359453073018	21.428502469373704
11	33.29525671978856	25.351223935283656	22.54961931109906	18.803900033828715
12	20.89498519582942	27.29725314764819	31.175532627681385	20.632229028841003
13	27.019655229274964	23.39075392867087	26.31797238760234	23.271618454451826
14	20.49210588509703	23.04917815011562	27.664814073937915	28.793901890849437
15	22.955500014674737	30.859429909588165	23.59369665925721	22.591373416479886
16	22.13528914730239	30.842533392856314	25.425954903617566	21.59622255622373
17	20.69309748648086	30.035563165843914	27.887909411823316	21.38342993585191
18	20.957437277369806	29.601590425318285	28.71110826199384	20.72986403531807
19	21.404416475415818	28.76428202635921	28.876078332331222	20.955223165893745
20	22.357051389980892	28.488892973711078	28.3855538375274	20.76850179878063
21	22.4663578170176	28.517796085800274	27.940587087446296	21.075259009735838
22	21.624692424849638	28.373394590078217	28.542162384220926	21.459750600851223
23	21.79631707233182	28.548389777091245	28.330037436798595	21.32525571377834
24	21.617677441528627	28.20879585559458	28.426172468589677	21.747354234287116
25	22.116172470955334	28.168439516614395	27.834692434433965	21.8806955779963
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2633.0
1	2633.0
2	2779.0
3	2925.0
4	2925.0
5	2925.0
6	3980.5
7	5036.0
8	5036.0
9	5036.0
10	7625.5
11	10215.0
12	10215.0
13	10215.0
14	16668.5
15	23122.0
16	23122.0
17	23122.0
18	35643.0
19	48164.0
20	48164.0
21	48164.0
22	68625.0
23	89086.0
24	89086.0
25	89086.0
26	116095.0
27	143104.0
28	143104.0
29	143104.0
30	171674.0
31	200244.0
32	200244.0
33	200244.0
34	225531.0
35	250818.0
36	250818.0
37	250818.0
38	269171.5
39	287525.0
40	287525.0
41	287525.0
42	291727.5
43	295930.0
44	295930.0
45	295930.0
46	301016.5
47	306103.0
48	306103.0
49	306103.0
50	292630.5
51	279158.0
52	279158.0
53	279158.0
54	255473.5
55	231789.0
56	231789.0
57	231789.0
58	204042.5
59	176296.0
60	176296.0
61	176296.0
62	148542.5
63	120789.0
64	120789.0
65	120789.0
66	93892.0
67	66995.0
68	66995.0
69	66995.0
70	48880.5
71	30766.0
72	30766.0
73	30766.0
74	21688.0
75	12610.0
76	12610.0
77	12610.0
78	8499.5
79	4389.0
80	4389.0
81	4389.0
82	2876.0
83	1363.0
84	1363.0
85	1363.0
86	852.0
87	341.0
88	341.0
89	341.0
90	225.5
91	110.0
92	110.0
93	110.0
94	66.5
95	23.0
96	23.0
97	23.0
98	15.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030198390294499954
2	0.014404091534333098
3	0.020273855376742297
4	0.022899802358872727
5	0.021084809591811988
6	0.008727412028845256
7	0.0012743566236809445
8	0.0015446746953708416
9	0.0014674409606022995
10	7.337204803011498E-4
11	0.001004038551991047
12	0.002664563849514702
13	0.002046693971366365
14	6.951036129168788E-4
15	0.0022397783082877204
16	0.002548713247361889
17	0.002510096379977618
18	0.0017763758996764678
19	4.247855412269815E-4
20	3.861686738427104E-4
21	0.08074786970051075
22	0.07580491067532406
23	0.07650001428824094
24	0.001042655419375318
25	0.2258700573306013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2589542.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.17168910547544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.39454329781793	63.33857516789813
2	10.034972998771147	14.886217948933881
3	2.3954688434572886	5.330279109563003
4	0.8194060629437924	2.431069270072344
5	0.3920026922285708	1.453775090824346
6	0.21357459762716188	0.9504713193617318
7	0.13959335919326374	0.7247712667490203
8	0.09628095102014904	0.5713056612676801
9	0.06852617523486529	0.45744319485969814
>10	0.4083340821543992	5.504782519631758
>50	0.02665583045832851	1.3381804841933826
>100	0.008969571563019347	1.1858075105456287
>500	6.790621216354853E-4	0.3201415739172088
>1k	9.402398606821042E-4	1.3005969374816735
>5k	5.2235547815672454E-5	0.20658294470053568
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5332	0.20590513689293322	No Hit
CCCATGTACTCTGCGTTGATACCAC	4740	0.18304395140144475	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3537	0.1365878599381667	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2612	0.10086725760771596	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.861686738427104E-5
2	0.0	0.0	0.0	3.861686738427104E-5	3.861686738427104E-5
3	0.0	0.0	0.0	3.861686738427104E-5	3.861686738427104E-5
4	0.0	0.0	0.0	1.5446746953708416E-4	3.861686738427104E-5
5	0.0	0.0	0.0	1.5446746953708416E-4	3.861686738427104E-5
6	0.0	0.0	0.0	1.5446746953708416E-4	3.861686738427104E-5
7	0.0	0.0	0.0	1.5446746953708416E-4	3.861686738427104E-5
8	0.0	0.0	0.0	1.5446746953708416E-4	3.861686738427104E-5
9	0.0	0.0	0.0	2.3170120430562625E-4	3.861686738427104E-5
10	0.0	0.0	0.0	2.3170120430562625E-4	3.861686738427104E-5
11	0.0	0.0	0.0	2.3170120430562625E-4	3.861686738427104E-5
12	0.0	0.0	0.0	2.3170120430562625E-4	3.861686738427104E-5
13	0.0	0.0	0.0	2.7031807168989726E-4	3.861686738427104E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	40	0.0052951216	14.244004	9
AGGACCG	60	4.1015327E-4	12.663782	5
GTCGTAC	65	8.0120174E-4	11.69484	1
ACCGAAC	60	0.005895041	11.078884	8
TGCGGGA	70	0.0014936165	10.85572	2
CAATCCG	70	0.0014972303	10.852574	10
AACACCG	80	3.7804485E-4	10.685067	5
ACCGCCC	80	3.7865248E-4	10.683209	8
GAATCGT	75	0.0026570004	10.129461	6
GTTCTAG	210	6.730261E-11	9.502058	1
GTGTAGG	370	0.0	9.502058	1
TAGCACC	200	2.6557245E-10	9.497837	4
TGTACCG	80	0.0045256563	9.497837	5
ACACCGT	110	6.8680674E-5	9.49637	6
TATCGGT	190	1.0604708E-9	9.496003	13
CTAGAAC	265	0.0	9.318993	3
CTAGCAC	215	1.07320375E-10	9.277316	3
TCGGTCA	195	1.6698323E-9	9.260562	15
ATCGGTC	195	1.6952981E-9	9.252695	14
GGACTGT	445	0.0	9.176268	6
>>END_MODULE