FastQCFastQC Report
Wed 25 May 2016
SRR1294757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589542
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104790.4046661533197762No Hit
GGTATCAACGCAGAGTACTTTTTTT83140.3210606354328295No Hit
TATCAACGCAGAGTACTTTTTTTTT66870.25823099219862045No Hit
GTACATGGGAAGCAGTGGTATCAAC55720.21517318506515823No Hit
CCCATGTACTCTGCGTTGATACCAC50580.19532411522964294No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44620.17230846226861737No Hit
GAGTACATGGGAAGCAGTGGTATCA30820.11901718527832335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG550.00304066712.1060985
TATACCG600.005822778711.0972575
GAACCGT600.00589611311.0786136
ACCGTTA700.001498259410.8516828
TACTCCG803.728987E-410.7009275
AGGACCG909.399357E-510.5688165
TACGCAG909.399357E-510.5688165
ACTAGAC1502.3996108E-810.1460643
ACACCGT951.6518676E-49.9957416
TGCACCG800.0044706219.5119355
CACACCG1502.6019916E-79.5119345
GGACCGC900.00111838869.49595456
CTATACT4400.09.2957544
CTACGCA1054.4379712E-49.0589864
ATACGAC1054.4379712E-49.0589863
TATACTG6200.09.051685
ACTGCGT950.0018326848.9954738
GACTGCG950.0018326848.9954737
GCTATAC2651.8189894E-128.9735233
CCGTGTG1804.2557076E-88.9677099
Produced by FastQC (version 0.11.5)