FastQCFastQC Report
Wed 25 May 2016
SRR1294756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607032
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC26560.16527362242942267No Hit
CCCATGTACTCTGCGTTGATACCAC23340.14523668477043394No Hit
GTATCAACGCAGAGTACTTTTTTTT21140.13154685158727392No Hit
GGTATCAACGCAGAGTACTTTTTTT16150.10049582086728825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG456.7703193E-414.7755275
AGTACCG400.00528454514.24782855
CGCCCTA500.001494982513.30542816
GACCGTG658.05074E-411.6876157
GTGAGCG600.005893381311.0788879
CGGGTTC951.3540244E-511.0044918
CCGGGTT1053.448944E-610.86157417
CTTAGAC1408.336428E-910.8581923
TTGGACT1158.8078923E-710.7398354
TCTGTCG1201.5312071E-610.2875388
GTACTAG1054.087481E-59.9561321
GTCTTAC1752.5538611E-99.7751121
TTAGACT2054.1836756E-119.7323464
TAGACAG2750.09.6712545
CTAAGAC1606.6211214E-89.5009173
GTAAGGC800.004511999.5009173
TAGACCC800.0045131499.5006234
TAGACCA1502.6443013E-79.5006234
CAGGACT2850.09.3339444
GTATTAG2750.09.3307891
Produced by FastQC (version 0.11.5)