FastQCFastQC Report
Wed 25 May 2016
SRR1294756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607032
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61900.38518212456254763No Hit
GGTATCAACGCAGAGTACTTTTTTT51930.32314228963704517No Hit
TATCAACGCAGAGTACTTTTTTTTT41660.2592356592774755No Hit
GTACATGGGAAGCAGTGGTATCAAC27940.17386088142613215No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25550.15898874446806285No Hit
CCCATGTACTCTGCGTTGATACCAC25200.15681081646165104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCT250.00605159818.9894249
CTAGGAC1459.458745E-1111.8096873
CTAGTAC1052.6707312E-711.7784443
TAGGACA1657.2759576E-1211.5313444
CCTATAC2000.011.4160313
GGACAGT1701.2732926E-1111.17163856
TTAGACT1201.2505734E-711.0989194
ACCGGGC600.005898848611.0775078
GTCCTAG1553.0195224E-1011.0532851
CGGTCAG1106.087026E-610.35754316
ATCGGTC1201.5348814E-610.28561614
ATAGGAC1303.7668542E-710.2451563
GCTACAC1303.7668542E-710.2451563
TATACTG3650.010.1649585
TAGACTG2250.010.1475825
CTAGACT1701.5206751E-910.07296854
GGGCTAG951.612611E-410.019061
GCCTAAG1151.0032414E-59.9319371
TATCGGT1252.5885838E-69.87419113
CTAATAC2450.09.7075093
Produced by FastQC (version 0.11.5)