FastQCFastQC Report
Wed 25 May 2016
SRR1294755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828318
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58950.20842776519471995No Hit
CCCATGTACTCTGCGTTGATACCAC53690.18983013932662451No Hit
GTATCAACGCAGAGTACTTTTTTTT37950.1341786885350233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG957.418748E-812.9968185
ACAGCGT752.0820754E-411.39588
CGAGTCT803.7621683E-410.69074315
CTAGGAC3200.010.68545153
ACACCGA1106.076383E-610.3598186
TCTATAC4700.010.3065353
TTAGACT2002.5465852E-119.9727354
AGCACCG1054.1185063E-59.94994455
CTAGTAC1951.6370905E-109.7417223
CTAGACA4550.09.6022164
CTAGACT2600.09.4978434
TAGGACT4400.09.4978434
GTATCGC900.00111786929.49656
GCGCGCC800.0045311359.49649911
CCAGGAC6250.09.4221943
CTTCTAG3450.09.3636691
TAGACAG5300.09.3184745
CAGGACT5100.09.311614
GTCTAGT2252.7284841E-119.2902291
TAGACTG4200.09.2715395
Produced by FastQC (version 0.11.5)