##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294755_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2828318
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219117157264495	33.0	33.0	33.0	27.0	33.0
2	31.23565949797724	33.0	33.0	33.0	27.0	33.0
3	31.202237867170524	33.0	33.0	33.0	27.0	33.0
4	34.765817705081254	37.0	37.0	37.0	27.0	37.0
5	34.92417684291512	37.0	37.0	37.0	33.0	37.0
6	35.09092966208185	37.0	37.0	37.0	33.0	37.0
7	35.07851203436106	37.0	37.0	37.0	33.0	37.0
8	34.9684059571802	37.0	37.0	37.0	33.0	37.0
9	34.70781644779689	37.0	37.0	37.0	27.0	37.0
10	34.79727809956306	37.0	37.0	37.0	27.0	37.0
11	35.005523070602386	37.0	37.0	37.0	33.0	37.0
12	34.93667402321804	37.0	37.0	37.0	33.0	37.0
13	34.863956952506754	37.0	37.0	37.0	33.0	37.0
14	36.085869764291004	40.0	37.0	40.0	27.0	40.0
15	36.38825796816341	40.0	37.0	40.0	27.0	40.0
16	36.40875990606431	40.0	37.0	40.0	27.0	40.0
17	36.362921001103835	40.0	37.0	40.0	27.0	40.0
18	36.369996230975445	40.0	37.0	40.0	27.0	40.0
19	36.47188293537007	40.0	37.0	40.0	27.0	40.0
20	36.35735444175655	40.0	37.0	40.0	27.0	40.0
21	36.30984670040639	40.0	37.0	40.0	27.0	40.0
22	36.1725622790648	40.0	37.0	40.0	27.0	40.0
23	35.803401173418266	37.0	37.0	40.0	27.0	40.0
24	36.105905700844104	40.0	37.0	40.0	27.0	40.0
25	34.990943026915645	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5938510356610536
1101	2	0.754341334645126
1101	3	0.5725035717856599
1101	4	0.6433026011583394
1101	5	0.7023835863608809
1101	6	0.5097686764935716
1101	7	0.5684084311107327
1101	8	0.6487514795341696
1101	9	0.8214837453668764
1101	10	0.8628518726831587
1101	11	0.6232487195580916
1101	12	0.648565980400825
1101	13	0.6794514317870295
1101	14	1.3256320404896371
1101	15	1.0294957655820838
1101	16	0.9754066179525864
1101	17	1.0202349974313094
1101	18	1.0000300712670125
1101	19	1.0298394295475717
1101	20	1.0672471958271998
1101	21	1.092745420880462
1101	22	1.2007185299661245
1101	23	1.5055267001850936
1101	24	1.2446050534728315
1101	25	1.155046994541145
1102	1	0.30439408447310967
1102	2	0.6145652536329287
1102	3	0.39767778941541465
1102	4	0.43234661212229497
1102	5	0.3663149611889551
1102	6	0.2898120523099621
1102	7	0.3628826708551216
1102	8	0.38313142320549076
1102	9	0.6266298316648999
1102	10	0.6367292010271015
1102	11	0.3923833062430049
1102	12	0.48079625369346246
1102	13	0.45421464245562504
1102	14	1.116646564951779
1102	15	0.8535563530959394
1102	16	0.5983755721092692
1102	17	0.7447775803741763
1102	18	0.7999168575099134
1102	19	0.7335235211993165
1102	20	0.8701964354183502
1102	21	0.8924639896812749
1102	22	0.9024441320714018
1102	23	1.253727704821621
1102	24	0.9825101368695499
1102	25	0.9220929500845898
1103	1	0.45416022002959977
1103	2	0.5671428187893284
1103	3	0.40055372134770906
1103	4	0.34009076539523875
1103	5	0.46702743247485756
1103	6	0.3452960030349175
1103	7	0.37685887202261625
1103	8	0.5725525768161361
1103	9	0.7016071248028339
1103	10	0.7426770072519773
1103	11	0.5647339558587277
1103	12	0.48267658597904983
1103	13	0.5366661810484743
1103	14	1.1784971347803648
1103	15	0.8290044135263415
1103	16	0.560931167970729
1103	17	0.7371663747348975
1103	18	0.7432569742960098
1103	19	0.8421716812708624
1103	20	0.9077849898610069
1103	21	0.9881445368354704
1103	22	0.894059640649445
1103	23	1.3771696522586012
1103	24	0.963010995972887
1103	25	0.971587067174994
1104	1	0.2894492690448871
1104	2	0.4736893695920479
1104	3	0.34502627618026693
1104	4	0.43471191158892
1104	5	0.3538258212606351
1104	6	0.22300806619797697
1104	7	0.34767592590549157
1104	8	0.41249639014350237
1104	9	0.6034472293042654
1104	10	0.5970037601967277
1104	11	0.37663453505810907
1104	12	0.3942444144147004
1104	13	0.4119706308451754
1104	14	0.9788886540593253
1104	15	0.757138463516597
1104	16	0.7494131257657273
1104	17	0.7105876140915655
1104	18	0.6486024266420571
1104	19	0.6680735658354209
1104	20	0.7680359882938532
1104	21	0.8507778718539285
1104	22	0.9302476403172335
1104	23	1.316257801420491
1104	24	1.105553081812836
1104	25	0.8270144885100379
1105	1	0.13368473739159725
1105	2	0.18766891582487233
1105	3	0.24108763569590153
1105	4	0.18364037921278964
1105	5	0.14336614726543218
1105	6	0.24820135501212093
1105	7	0.15719463820931878
1105	8	0.14612627750312157
1105	9	-1.6441201689660119
1105	10	-0.19584023406521567
1105	11	-0.02727144601238507
1105	12	0.1308531617799602
1105	13	0.13054046551219756
1105	14	-1.6940151495680809
1105	15	-0.4270195553629108
1105	16	-0.10703669548927763
1105	17	0.03605441154471123
1105	18	0.05157081688343368
1105	19	0.1446335718717151
1105	20	0.26158458206386825
1105	21	0.4306215442096857
1105	22	0.4523820390514004
1105	23	0.8110180081012288
1105	24	0.6604490248573214
1105	25	0.3439218183719035
1106	1	0.09996312815437491
1106	2	0.05398560003470365
1106	3	0.060965638713526005
1106	4	-1.0938830499738614
1106	5	-0.16678971304919799
1106	6	-0.08284713221603823
1106	7	-0.03187121059695386
1106	8	0.08442628559996734
1106	9	0.30950947050079236
1106	10	0.35818034637581064
1106	11	0.2398535788738414
1106	12	0.24802173685154827
1106	13	0.21197902982525108
1106	14	0.6251568921276274
1106	15	0.4456195000458152
1106	16	0.20813728899752704
1106	17	0.04758005025535539
1106	18	0.19731690727606832
1106	19	0.014223267354680047
1106	20	0.26231002324311703
1106	21	0.5152790279022241
1106	22	0.5897002962173588
1106	23	0.7151356659877308
1106	24	0.35478013793862573
1106	25	0.5034679478597681
1107	1	0.3641184794992256
1107	2	0.41420099058016646
1107	3	0.3046121506417876
1107	4	0.5063143606725049
1107	5	0.3389641281762579
1107	6	0.23892581712183159
1107	7	0.21192086433156732
1107	8	0.4555647050059335
1107	9	0.5043448125301424
1107	10	0.4528915595900429
1107	11	0.2873126539563984
1107	12	0.28285925899036357
1107	13	0.34482167842342903
1107	14	0.8785599943006446
1107	15	0.5471284119770701
1107	16	0.6264026303337573
1107	17	0.6880218946679904
1107	18	0.7071749313163807
1107	19	0.5545553924884317
1107	20	0.760219406339516
1107	21	0.724489416670842
1107	22	0.753933785940859
1107	23	0.9316244716126292
1107	24	0.8629265625757796
1107	25	0.955820114523668
1108	1	0.3566229867269861
1108	2	0.37074953686823875
1108	3	0.40562542751543873
1108	4	0.5788880623400701
1108	5	0.4063462782207452
1108	6	0.44730427565311714
1108	7	0.2911822822714427
1108	8	0.45764447547547604
1108	9	0.06206037994550684
1108	10	0.39860281886407734
1108	11	0.2630109646747414
1108	12	0.3127673109090594
1108	13	0.3199986811788875
1108	14	-0.9874927537588221
1108	15	-0.08151285333623548
1108	16	0.24019300090122186
1108	17	0.4869243474000484
1108	18	0.5874262564953199
1108	19	0.5248647162740809
1108	20	0.6239072489856667
1108	21	0.4908041205528022
1108	22	0.6288012680468995
1108	23	1.0033782674318772
1108	24	0.7720482770941075
1108	25	0.6825458953584089
1109	1	0.3693491924417849
1109	2	0.41419054241781694
1109	3	0.3932272073071168
1109	4	0.6020058263131887
1109	5	0.5913122118956977
1109	6	0.36424163941848775
1109	7	0.16587137082344583
1109	8	0.3845823299922273
1109	9	0.4937644527730143
1109	10	0.4677710566167619
1109	11	0.3551846047822167
1109	12	0.21535111693139442
1109	13	0.24577141012463244
1109	14	0.7561615241618682
1109	15	0.36927347351236506
1109	16	0.4064456642498655
1109	17	0.3761363029216156
1109	18	0.4717588141688296
1109	19	0.49268706836365794
1109	20	0.6103662937381031
1109	21	0.6270360905515346
1109	22	0.5686604019524992
1109	23	1.0517824052839373
1109	24	0.7845022180321735
1109	25	0.6406802017426756
1110	1	0.2588327809588158
1110	2	0.20938385376181046
1110	3	0.24522135806332201
1110	4	0.49305496234687496
1110	5	0.327682648759847
1110	6	0.2139880959736331
1110	7	0.1950173852059791
1110	8	0.19206302967471345
1110	9	0.3497216053722667
1110	10	0.30001799530706563
1110	11	0.1146795359015016
1110	12	0.16006144814333112
1110	13	0.24109136892941052
1110	14	0.6340396225015326
1110	15	0.317282354740108
1110	16	0.22185927656548898
1110	17	0.31451272024984434
1110	18	0.2946017812583861
1110	19	0.2775042347609258
1110	20	0.4531991310189838
1110	21	0.482989117664431
1110	22	0.668613132825655
1110	23	0.7183354968140705
1110	24	0.6414393476082338
1110	25	0.5208015705691054
1111	1	0.1296039064340171
1111	2	0.07875380446258262
1111	3	0.14055851881617087
1111	4	0.3771003751587756
1111	5	0.3613489454672134
1111	6	0.2113556081386605
1111	7	0.2071667996009623
1111	8	0.31119115481683224
1111	9	0.38649261039559946
1111	10	0.13489102306138534
1111	11	0.09672925794038889
1111	12	0.2086541893205407
1111	13	0.2211118457467336
1111	14	-1.6664447660761894
1111	15	-0.5293185058761836
1111	16	0.03459153050260255
1111	17	0.15240638583367883
1111	18	0.2099580422357903
1111	19	0.26206205964603413
1111	20	0.3791020591055343
1111	21	0.38952510634687343
1111	22	0.28627752196876344
1111	23	-1.8110258747639634
1111	24	-0.44719331475904056
1111	25	0.126414135911169
1112	1	-0.12889374496337425
1112	2	-0.20264469994416956
1112	3	0.03981819579171031
1112	4	0.16872504327519522
1112	5	-0.636185592021377
1112	6	-0.8123629730229212
1112	7	-0.9388097595057374
1112	8	-0.679573612698448
1112	9	-0.5254106482389531
1112	10	-0.8059084675691963
1112	11	-0.7636961936420974
1112	12	-0.7320178618959332
1112	13	-0.7335160345611627
1112	14	-0.47531219074692643
1112	15	-0.7685664483314198
1112	16	-0.8184811133605265
1112	17	-0.8613934197557
1112	18	-0.8688026215944689
1112	19	-0.686609168503999
1112	20	-0.762659390423174
1112	21	-1.7373592232155275
1112	22	-1.741227407451838
1112	23	-1.283597040164885
1112	24	-1.559585608799523
1112	25	-1.86642605962534
1113	1	-0.03168218744142948
1113	2	-0.15637827831895734
1113	3	-0.2768369595582669
1113	4	0.028840178840745523
1113	5	-0.14578972591998962
1113	6	-0.22279841757493557
1113	7	-0.1478876526420052
1113	8	-0.09941640283042119
1113	9	0.06506441316140155
1113	10	-0.18327771493067502
1113	11	-0.28170597258161933
1113	12	-0.17597394066309846
1113	13	-0.24803464473646386
1113	14	-0.1890715339923119
1113	15	-0.23315727022023225
1113	16	-0.053318597407475465
1113	17	-0.15526639145610233
1113	18	-0.04715888236682986
1113	19	-0.1476839111072792
1113	20	-0.8334684351982702
1113	21	-0.5786527080342694
1113	22	-0.21224576780196713
1113	23	-0.35892204087284796
1113	24	-0.1313025676808408
1113	25	-1.2232463827709594
1114	1	-0.9909400938921031
1114	2	-0.6958672622528717
1114	3	-0.5738678558042665
1114	4	-1.5210432120811674
1114	5	-0.7750137511818522
1114	6	-0.7347503801064406
1114	7	-0.5664879405289156
1114	8	-0.4926475086319826
1114	9	-0.43008926651616974
1114	10	-0.5988686774511365
1114	11	-0.5127123720417472
1114	12	-0.6229229777682548
1114	13	-0.6189554748759818
1114	14	-0.5050454657797232
1114	15	-1.2655056700192162
1114	16	-0.8864741172958119
1114	17	-0.8933129650114964
1114	18	-0.7977488393469798
1114	19	-0.8916813154213585
1114	20	-2.2073467537812803
1114	21	-6.976537185611679
1114	22	-8.096496318689265
1114	23	-8.17887469648321
1114	24	-6.107246299660915
1114	25	-7.758424310609165
1115	1	-1.0940999680783108
1115	2	-1.0748505097426815
1115	3	-0.8534852442900132
1115	4	-0.6332132469055054
1115	5	-0.7931460102783703
1115	6	-0.9367375658258013
1115	7	-1.0767232365740327
1115	8	-0.7927476542296361
1115	9	-2.591541668072942
1115	10	-1.7279386252465159
1115	11	-1.240811335277776
1115	12	-0.9937909785519139
1115	13	-1.3793360399196573
1115	14	-1.3156028700152476
1115	15	-2.1996648938672934
1115	16	-1.4434130897968842
1115	17	-3.1162874619265324
1115	18	-2.7409055682864576
1115	19	-2.141586766714724
1115	20	-1.8809903219822672
1115	21	-1.4158040507709515
1115	22	-0.9260126639164881
1115	23	-0.4298994769266429
1115	24	-1.3741053764201396
1115	25	-1.1319579717636685
1116	1	-1.539231551941608
1116	2	-0.9071841016731739
1116	3	-0.6941517516560367
1116	4	-0.3003474096810592
1116	5	-0.4091532075773472
1116	6	-0.47028984763769444
1116	7	-0.8276259457705066
1116	8	-1.806635530285206
1116	9	-0.8623214114244178
1116	10	-2.308945300089995
1116	11	-1.0980078650544485
1116	12	-0.7660085234266489
1116	13	-0.7799945236479147
1116	14	-1.2985447479900216
1116	15	-1.2541234371734618
1116	16	-0.8085001960147835
1116	17	-0.7998160384440851
1116	18	-1.3284249555810206
1116	19	-0.8369000611992092
1116	20	-1.1436746024251931
1116	21	-0.6494078888509023
1116	22	-0.19587271577044874
1116	23	-0.08197123773752679
1116	24	-0.20657207962206314
1116	25	-0.10405270463306948
1201	1	0.32354306776018404
1201	2	0.38569542509609533
1201	3	0.27853971627200025
1201	4	0.4357002349218817
1201	5	0.3784498003617216
1201	6	0.29462932711376055
1201	7	0.2975707363985407
1201	8	0.48131773066214834
1201	9	0.6235204986920664
1201	10	0.5770311789631037
1201	11	0.4417758222554653
1201	12	0.42796310039875607
1201	13	0.4821956826897065
1201	14	1.0808877285180216
1201	15	0.8604334537478664
1201	16	0.8380656892466476
1201	17	0.7540769995858412
1201	18	0.6921917105794932
1201	19	0.5981201745794138
1201	20	0.7612851436979184
1201	21	0.8204126936872242
1201	22	0.9553324157945013
1201	23	0.7031459135847271
1201	24	0.7745796682238009
1201	25	0.7214131472648546
1202	1	-0.22955157543708893
1202	2	0.08588165750325416
1202	3	-0.016250098007791536
1202	4	-0.07812834162868398
1202	5	0.036327583243711103
1202	6	0.013538438976453904
1202	7	0.03076660295145217
1202	8	0.10490017951826758
1202	9	0.2448344655623842
1202	10	0.3344936803836518
1202	11	0.2052586643844876
1202	12	0.10426644713996325
1202	13	0.2722596595639146
1202	14	0.4283832347708483
1202	15	0.46379844205532095
1202	16	0.4543873150947775
1202	17	0.22658991260642125
1202	18	0.29439144583655974
1202	19	0.1962367222931647
1202	20	0.37218506158599496
1202	21	0.413118737797177
1202	22	0.456059898639829
1202	23	0.6256429804443258
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2211	5	0.3153427022282571
2211	6	0.23761909568521133
2211	7	0.2258429668350601
2211	8	0.3205370389688156
2211	9	0.4371909339599185
2211	10	0.40753241537019846
2211	11	0.2600387274149938
2211	12	0.2957094709854431
2211	13	0.20792819210734592
2211	14	0.5936876603024572
2211	15	0.486867065336142
2211	16	0.26770112356766873
2211	17	0.36101873860150846
2211	18	0.27308151697353367
2211	19	0.33222016454238457
2211	20	0.42582534250762905
2211	21	0.39388712800190717
2211	22	0.5663989011033337
2211	23	0.5704750980775302
2211	24	0.561673668083067
2211	25	0.7350593305708273
2212	1	-0.23231904276079618
2212	2	-0.24270102084549805
2212	3	-0.18180902628636986
2212	4	-0.5255123153152539
2212	5	-0.19347917696629224
2212	6	-0.5645759038486844
2212	7	-0.4840107199728507
2212	8	-0.35736112739201786
2212	9	-0.22414543380404695
2212	10	-0.49100945430380705
2212	11	-0.5266132808810369
2212	12	-0.6446613982469813
2212	13	-0.7260449172168109
2212	14	-0.44283322641609146
2212	15	-0.6686518832157446
2212	16	-0.4921677334472392
2212	17	-0.5149515367450448
2212	18	-0.3404292892932901
2212	19	-0.5381794628874559
2212	20	-0.4850187963140655
2212	21	-0.5207868312625337
2212	22	-0.4575205938484572
2212	23	0.007989317105732141
2212	24	-0.2733913458386894
2212	25	-0.9322787564183912
2213	1	-0.2545522879974982
2213	2	-1.3579501365985216
2213	3	-0.369465787112329
2213	4	-0.18222240041117743
2213	5	-0.07181134238499709
2213	6	-0.1774533218037817
2213	7	-0.06796278560702973
2213	8	0.045083007358215355
2213	9	0.03327694798943526
2213	10	-0.5028806285085565
2213	11	-0.21187647284858002
2213	12	-0.1013247667577275
2213	13	-0.5159449489735906
2213	14	-0.18144562135844922
2213	15	-0.2606301906231252
2213	16	-0.26643520601874116
2213	17	-0.04749654957461047
2213	18	-0.055662395430047695
2213	19	-0.08460253843097121
2213	20	0.06614592514048212
2213	21	-0.6201391854197453
2213	22	-1.0201028837134984
2213	23	-0.2951951967837019
2213	24	-0.13566052960196373
2213	25	-0.787966938084665
2214	1	-0.30813115267389435
2214	2	-0.5599702704087548
2214	3	-0.49137910683504415
2214	4	-0.7166507357065726
2214	5	-0.4378326676870188
2214	6	-0.39649648186809827
2214	7	-0.6663197574471553
2214	8	-0.28330053294804713
2214	9	-0.16924814424238122
2214	10	-2.0338522870606184
2214	11	-0.9662731000564619
2214	12	-0.6885568968194136
2214	13	-0.41957367172891225
2214	14	-0.9625120065089234
2214	15	-0.6942424140683485
2214	16	-0.8169936990367361
2214	17	-0.7432279627153875
2214	18	-0.6693954637539932
2214	19	-1.633683671668713
2214	20	-2.5584501268799897
2214	21	-2.021542864788401
2214	22	-2.190868867373254
2214	23	-2.8336737037874684
2214	24	-1.7642542316524867
2214	25	-1.972699936629816
2215	1	-0.26133648178791447
2215	2	-0.337806234035547
2215	3	-0.5880350253894591
2215	4	-0.3194888128504303
2215	5	-0.27654190238159515
2215	6	-0.29188700446471927
2215	7	-0.34809638320861325
2215	8	-0.31832088572198103
2215	9	-0.3151774542521508
2215	10	-0.3323380394556068
2215	11	-0.5673285779789197
2215	12	-0.5785433973509697
2215	13	-0.4223547387588553
2215	14	-0.28078012557465115
2215	15	-0.796780364135877
2215	16	-0.5573308954421918
2215	17	-0.4411558894620313
2215	18	-0.48331336010834036
2215	19	-2.206473428991039
2215	20	-2.2507884706305674
2215	21	-3.3297530915987537
2215	22	-2.572084744021609
2215	23	-3.3156997781339825
2215	24	-2.136370530856297
2215	25	-1.6191628937547335
2216	1	-1.6576027145239252
2216	2	-1.3196786571854844
2216	3	-0.9521790295693222
2216	4	-1.1115597392396168
2216	5	-2.0788592062003417
2216	6	-1.7271473631193928
2216	7	-1.3048916047686205
2216	8	-5.340984762384892
2216	9	-4.213141442770105
2216	10	-2.2525017956200983
2216	11	-1.3124710495867902
2216	12	-1.4489370340696723
2216	13	-2.2071758302138207
2216	14	-3.8102460131423683
2216	15	-2.230002608158827
2216	16	-3.072725662669413
2216	17	-2.844640436110808
2216	18	-2.884691400424934
2216	19	-1.737368933867856
2216	20	-1.16766239719162
2216	21	-1.074977119260474
2216	22	-1.0822434552492481
2216	23	-1.1960171345052473
2216	24	-0.7910483002651816
2216	25	-0.6502242117918229
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	46.0
8	176.0
9	566.0
10	1198.0
11	2236.0
12	3923.0
13	6014.0
14	8130.0
15	10474.0
16	12008.0
17	12812.0
18	13613.0
19	14079.0
20	14451.0
21	15682.0
22	17245.0
23	18345.0
24	19709.0
25	21408.0
26	23380.0
27	26418.0
28	30622.0
29	36319.0
30	43626.0
31	54165.0
32	72846.0
33	104002.0
34	151724.0
35	240761.0
36	421201.0
37	1431134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88494213082494	20.140043262177567	13.410001796696061	23.565012810301432
2	15.614962861593353	19.62842144533025	40.60330240970885	24.153313283367545
3	18.95787978707904	24.400540931267496	28.63777691960579	28.003802362047676
4	12.271966135081277	16.350397230262704	37.50861564413544	33.86902099052058
5	13.58753849396474	37.511723242362386	34.88426701974314	14.016471243929729
6	32.85518548442702	37.246878243082456	16.70751219443202	13.190424078058507
7	27.859186730747272	32.046099565359285	21.865295672720805	18.229418031172646
8	24.693721692459455	37.481817763965054	19.870063748316134	17.95439679525936
9	24.155614381647766	17.22660864356986	21.402510837786245	37.21526613699612
10	14.660592367880465	30.375524606567172	33.17334379894707	21.790539226605286
11	33.492416276384276	25.029399348158286	22.278730570442818	19.199453805014613
12	21.354639568925283	26.78942678942679	30.99908778480207	20.856845856845858
13	27.427407253405296	22.97344217825619	26.120483239777048	23.478667328561468
14	20.6309225001706	22.60132666125235	27.600861718139818	29.16688912043724
15	23.210124917352303	30.508791593447587	23.34310373479194	22.937979754408172
16	22.272551610408815	30.5230748125082	25.321284451872657	21.883089125210333
17	20.92069426505441	29.618630838710907	27.753913452702495	21.706761443532184
18	21.188958849552517	29.360072072683664	28.461956767704194	20.989012310059625
19	21.683246661961853	28.387396280744532	28.695601752001547	21.233755305292068
20	22.59469554336685	28.05578331302079	28.22404687478454	21.125474268827823
21	22.715893252127252	28.046457595925922	27.711503750272907	21.526145401673922
22	21.806723729791127	27.852772892774873	28.530064293321917	21.810439084112083
23	21.97753553264736	28.072268735693434	28.19102051157388	21.75917522008533
24	21.854967991214465	27.867301963097084	28.23282369604771	22.044906349640737
25	22.182635670562085	27.87381473635569	27.679197263165207	22.264352329917013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1412.0
1	1412.0
2	1736.0
3	2060.0
4	2060.0
5	2060.0
6	3257.0
7	4454.0
8	4454.0
9	4454.0
10	7106.0
11	9758.0
12	9758.0
13	9758.0
14	16445.5
15	23133.0
16	23133.0
17	23133.0
18	36418.0
19	49703.0
20	49703.0
21	49703.0
22	71153.5
23	92604.0
24	92604.0
25	92604.0
26	121495.0
27	150386.0
28	150386.0
29	150386.0
30	181577.5
31	212769.0
32	212769.0
33	212769.0
34	240027.5
35	267286.0
36	267286.0
37	267286.0
38	287327.0
39	307368.0
40	307368.0
41	307368.0
42	313435.0
43	319502.0
44	319502.0
45	319502.0
46	326905.5
47	334309.0
48	334309.0
49	334309.0
50	321732.5
51	309156.0
52	309156.0
53	309156.0
54	284185.0
55	259214.0
56	259214.0
57	259214.0
58	230753.5
59	202293.0
60	202293.0
61	202293.0
62	171566.5
63	140840.0
64	140840.0
65	140840.0
66	110441.0
67	80042.0
68	80042.0
69	80042.0
70	58997.5
71	37953.0
72	37953.0
73	37953.0
74	27095.0
75	16237.0
76	16237.0
77	16237.0
78	10846.0
79	5455.0
80	5455.0
81	5455.0
82	3611.0
83	1767.0
84	1767.0
85	1767.0
86	1111.0
87	455.0
88	455.0
89	455.0
90	291.0
91	127.0
92	127.0
93	127.0
94	74.5
95	22.0
96	22.0
97	22.0
98	17.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03203317307318342
2	0.013541617314601824
3	0.021284735309113047
4	0.021673659043997175
5	0.021284735309113047
6	0.006470276680344996
7	1.7678351585642067E-4
8	2.828536253702731E-4
9	6.717773602543986E-4
10	2.828536253702731E-4
11	8.485608761108192E-4
12	0.0013435547205087972
13	0.002616396034675026
14	5.30350547569262E-4
15	0.0016971217522216385
16	0.002156758893448332
17	0.002156758893448332
18	0.0021921155966196162
19	3.5356703171284134E-4
20	6.010639539118303E-4
21	0.07980007905758829
22	0.07806760060219536
23	0.07972936565124572
24	0.0011314145014810924
25	0.2252929126074225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2828318.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.05018948366764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.08661152930848	67.10039919823775
2	9.24473842601247	14.246176949024084
3	1.9591047922462912	4.5284818638281426
4	0.6407852750860301	1.9749050745489087
5	0.31016056011481313	1.1948964963603421
6	0.1626376477752768	0.7518756946957845
7	0.10527689407600768	0.5678123245765879
8	0.08076466613394326	0.49783462633643866
9	0.05908536888553068	0.40972849815082857
>10	0.3180936391093875	4.509219426115162
>50	0.022686245327903424	1.2073573754318143
>100	0.008487444686239323	1.2123384935191466
>500	7.376523472882314E-4	0.36922250939089424
>1k	7.376523470817158E-4	1.0296242478494355
>5k	9.220654338521447E-5	0.40012722193465733
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5895	0.20842776519471995	No Hit
CCCATGTACTCTGCGTTGATACCAC	5369	0.18983013932662451	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3795	0.1341786885350233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.535670317128414E-5	0.0
5	0.0	0.0	0.0	3.535670317128414E-5	0.0
6	0.0	0.0	0.0	3.535670317128414E-5	0.0
7	0.0	0.0	0.0	3.535670317128414E-5	0.0
8	0.0	0.0	0.0	3.535670317128414E-5	0.0
9	0.0	0.0	0.0	3.535670317128414E-5	0.0
10	0.0	0.0	0.0	1.4142681268513655E-4	0.0
11	0.0	0.0	0.0	1.4142681268513655E-4	0.0
12	0.0	0.0	0.0	1.4142681268513655E-4	0.0
13	0.0	0.0	0.0	2.121402190277048E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	95	7.418748E-8	12.996818	5
ACAGCGT	75	2.0820754E-4	11.3958	8
CGAGTCT	80	3.7621683E-4	10.690743	15
CTAGGAC	320	0.0	10.6854515	3
ACACCGA	110	6.076383E-6	10.359818	6
TCTATAC	470	0.0	10.306535	3
TTAGACT	200	2.5465852E-11	9.972735	4
AGCACCG	105	4.1185063E-5	9.9499445	5
CTAGTAC	195	1.6370905E-10	9.741722	3
CTAGACA	455	0.0	9.602216	4
CTAGACT	260	0.0	9.497843	4
TAGGACT	440	0.0	9.497843	4
GTATCGC	90	0.0011178692	9.4965	6
GCGCGCC	80	0.004531135	9.496499	11
CCAGGAC	625	0.0	9.422194	3
CTTCTAG	345	0.0	9.363669	1
TAGACAG	530	0.0	9.318474	5
CAGGACT	510	0.0	9.31161	4
GTCTAGT	225	2.7284841E-11	9.290229	1
TAGACTG	420	0.0	9.271539	5
>>END_MODULE