FastQCFastQC Report
Wed 25 May 2016
SRR1294755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828318
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105380.37258893801899223No Hit
GGTATCAACGCAGAGTACTTTTTTT79610.281474713946593No Hit
TATCAACGCAGAGTACTTTTTTTTT73980.26156889006116No Hit
GTACATGGGAAGCAGTGGTATCAAC61000.21567588934483323No Hit
CCCATGTACTCTGCGTTGATACCAC56410.1994471625892138No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39200.1385982764314338No Hit
GAGTACATGGGAAGCAGTGGTATCA30260.10698938379630578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC701.0967667E-412.2080018
TAACGCA657.934677E-411.708444
ACACCGT951.3681591E-510.9949236
GTCCTAG3300.010.3829011
ACGGGAC750.002618594310.1473153
CTAGGAC2650.010.0515853
AGTACCG1201.6738597E-59.5131085
GCTAGAC1502.5977351E-79.5131073
CACCGTT900.00111936399.4951117
GTATTAG3650.09.3872811
CTAGACT3150.09.3621064
TAGGACC1951.6425474E-99.2691814
CAGGACT5150.09.2360274
GGTCTAG1651.036442E-79.2292451
CTAATAC3300.09.2248323
CTAGAAC3050.09.045253
GTACTAG1804.096546E-88.9889011
CAGTACT4400.08.8644874
CTAGCAC3550.08.843173
TAGCACC2054.0417945E-98.8170264
Produced by FastQC (version 0.11.5)