FastQCFastQC Report
Wed 25 May 2016
SRR1294754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4939109
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC2027194.1043637627758365No Hit
CCCATGTACTCTGCGTTGATACCAC1970303.9891810445973146No Hit
GAGTACATGGGAAGCAGTGGTATCA890701.8033616994482204No Hit
CATGTACTCTGCGTTGATACCACTG703801.4249533670951582No Hit
GCGTTGATACCACTGCTTCCCATGT627961.2714034049461147No Hit
GCTTCCCATGTACTCTGCGTTGATA528801.0706384491615797No Hit
ACGCAGAGTACATGGGAAGCAGTGG511891.0364015048058264No Hit
GTATCAACGCAGAGTACATGGGAAG497141.0065378188657104No Hit
ACTCTGCGTTGATACCACTGCTTCC426040.8625847293509822No Hit
TATCAACGCAGAGTACATGGGAAGC409950.8300080034678319No Hit
GGTATCAACGCAGAGTACATGGGAA331610.6713963996340231No Hit
CAGTGGTATCAACGCAGAGTACATG267500.5415956602698989No Hit
GTGGTATCAACGCAGAGTACATGGG260120.5266536940164714No Hit
GCAGTGGTATCAACGCAGAGTACAT218320.44202304504719375No Hit
ATACCACTGCTTCCCATGTACTCTG217060.43947197763807194No Hit
GTACTCTGCGTTGATACCACTGCTT204080.4131919340107699No Hit
ACATGGGAAGCAGTGGTATCAACGC196610.3980677486566909No Hit
CATGGGAAGCAGTGGTATCAACGCA194540.3938767093417051No Hit
GTACATGGTAAGCAGTGGTATCAAC185100.37476395034003096No Hit
GGGAAGCAGTGGTATCAACGCAGAG184550.3736503891693826No Hit
CTGCTTCCCATGTACTCTGCGTTGA183730.37199017069677953No Hit
CTTCCCATGTACTCTGCGTTGATAC171640.347512071509254No Hit
TACCACTGCTTCCCATGTACTCTGC167390.3389072806451528No Hit
GATACCACTGCTTCCCATGTACTCT163360.33074791424931094No Hit
GTTGATACCACTGCTTCCCATGTAC152950.30967123827394777No Hit
GCAGAGTACATGGGAAGCAGTGGTA152260.30827422516895253No Hit
AAGCAGTGGTATCAACGCAGAGTAC147050.29772576389790145No Hit
CCACTGCTTCCCATGTACTCTGCGT143900.291348095375097No Hit
CCATGTACTCTGCGTTGATACCACT137960.27932163473209437No Hit
ACCATGTACTCTGCGTTGATACCAC132430.2681252833253933No Hit
GTACATGGGTAAGCAGTGGTATCAA130690.2646023807127966No Hit
TTCCCATGTACTCTGCGTTGATACC125400.25389194690783295No Hit
ATGGGAAGCAGTGGTATCAACGCAG124670.2524139475358815No Hit
TGATACCACTGCTTCCCATGTACTC113380.2295555736874809No Hit
GGTAAGCAGTGGTATCAACGCAGAG95780.19392161622673237No Hit
ATCAACGCAGAGTACATGGGAAGCA87720.1776028834350487No Hit
CCCCATGTACTCTGCGTTGATACCA82960.16796551766725537No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA77950.1578219877309855No Hit
GCTTACCATGTACTCTGCGTTGATA68110.13789936605974884No Hit
AAAAAGTACTCTGCGTTGATACCAC67640.13694777742301295No Hit
GGAAGCAGTGGTATCAACGCAGAGT62930.1274116444889149No Hit
TCCATGTACTCTGCGTTGATACCAC60200.12188433176915107No Hit
GAGTACATGGTAAGCAGTGGTATCA57390.11619504651547476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA10100.011.6654561
AACCGCC600.005898397411.0783617
GAACAAA23400.010.6386591
GTCCTAT2500.010.2618381
TTAGACT2250.010.1318654
CTTAGAC2750.010.0173383
GTCGTAC951.6424978E-410.0017911
AAAAGTA15000.09.4994892
TGAACCG1002.763833E-49.4978525
GACTGCG1002.7700228E-49.4957387
CTCGATC1856.7648216E-99.23890913
GTCTTAA3200.09.2047731
AGTACTC17900.09.179495
CGTTGTT1451.7193834E-69.16811314
CGCAAAA4250.09.1642122
TTTAGAC2201.6916601E-109.0674183
GTTCTAG4000.09.0266171
TCGATCT1901.0682925E-88.9957814
AGCGGTG2850.08.9957811
AAGCGGT2750.08.97760710
Produced by FastQC (version 0.11.5)