FastQCFastQC Report
Wed 25 May 2016
SRR1294754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4939109
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC2153384.359855188456056No Hit
GTACATGGGAAGCAGTGGTATCAAC1929073.905704449932164No Hit
GAGTACATGGGAAGCAGTGGTATCA936241.895564564377907No Hit
CATGTACTCTGCGTTGATACCACTG799551.6188142436216735No Hit
GCGTTGATACCACTGCTTCCCATGT667571.351600055799538No Hit
ACGCAGAGTACATGGGAAGCAGTGG625801.2670301465304774No Hit
GCTTCCCATGTACTCTGCGTTGATA600321.2154418944793484No Hit
GTATCAACGCAGAGTACATGGGAAG520171.0531656620657694No Hit
ACTCTGCGTTGATACCACTGCTTCC486120.9842261023192646No Hit
TATCAACGCAGAGTACATGGGAAGC466250.943996174208749No Hit
GGTATCAACGCAGAGTACATGGGAA365320.7396475761113999No Hit
CAGTGGTATCAACGCAGAGTACATG317830.6434966306675961No Hit
GTGGTATCAACGCAGAGTACATGGG276190.5591899267661434No Hit
GCAGTGGTATCAACGCAGAGTACAT247240.50057611605656No Hit
CATGGGAAGCAGTGGTATCAACGCA246880.4998472396539538No Hit
ACATGGGAAGCAGTGGTATCAACGC246390.49885515788373974No Hit
ATACCACTGCTTCCCATGTACTCTG238080.4820302609235796No Hit
GGGAAGCAGTGGTATCAACGCAGAG215470.4362527735265612No Hit
CTGCTTCCCATGTACTCTGCGTTGA207730.4205819308705274No Hit
CTTCCCATGTACTCTGCGTTGATAC203140.4112887567372982No Hit
GTACTCTGCGTTGATACCACTGCTT199740.4044049240460172No Hit
GATACCACTGCTTCCCATGTACTCT187430.37948140039023237No Hit
TACCACTGCTTCCCATGTACTCTGC183830.37219263636417016No Hit
AAGCAGTGGTATCAACGCAGAGTAC178000.3603888879552972No Hit
GCAGAGTACATGGGAAGCAGTGGTA176490.3573316563776989No Hit
GTACATGGTAAGCAGTGGTATCAAC169100.34236944355753235No Hit
CCACTGCTTCCCATGTACTCTGCGT166320.3367408980040732No Hit
GTTGATACCACTGCTTCCCATGTAC163590.3312135852843094No Hit
CCATGTACTCTGCGTTGATACCACT163500.3310313661836578No Hit
ATGGGAAGCAGTGGTATCAACGCAG153340.3104608543767712No Hit
ACCATGTACTCTGCGTTGATACCAC150610.30493354165700737No Hit
GTACATGGGTAAGCAGTGGTATCAA132160.2675786260234386No Hit
TTCCCATGTACTCTGCGTTGATACC129750.2626992034393248No Hit
TGATACCACTGCTTCCCATGTACTC116180.23522461237441813No Hit
GTATCAACGCAGAGTACTTTTTTTT114780.2323900930309495No Hit
GGTAAGCAGTGGTATCAACGCAGAG108610.21989796135294848No Hit
ATCAACGCAGAGTACATGGGAAGCA102860.20825618547798802No Hit
CCCCATGTACTCTGCGTTGATACCA96360.19509591709759797No Hit
TATCAACGCAGAGTACTTTTTTTTT93340.18898145394240135No Hit
GGTATCAACGCAGAGTACTTTTTTT85140.1723792692163708No Hit
AAAAAGTACTCTGCGTTGATACCAC78850.15964417873750103No Hit
GCTTACCATGTACTCTGCGTTGATA75830.15352971558230444No Hit
GGAAGCAGTGGTATCAACGCAGAGT74260.15035100460427173No Hit
GTACTTTTTTTTTTTTTTTTTTTTT70910.14356840474668608No Hit
ACGCAGAGTACTTTTTTTTTTTTTT66810.13526731238367082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA66540.13472065508171616No Hit
GAGTACATGGTAAGCAGTGGTATCA60800.12309912577349479No Hit
TCCATGTACTCTGCGTTGATACCAC59650.12077077059850269No Hit
TGGGAAGCAGTGGTATCAACGCAGA57070.11554715637982478No Hit
ACTCTGCGTTGATACCACTGCTTAC55850.11307707523765925No Hit
CTCTGCGTTGATACCACTGCTTCCC54360.11006033679353908No Hit
CTGCGTTGATACCACTGCTTCCCAT53880.10908850159006413No Hit
GCTTACCCATGTACTCTGCGTTGAT50740.10273107963399877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG1408.169081E-910.8738275
ACACCGT1408.434654E-910.8516996
CCACGTA2150.010.6209482
GTATACG909.334314E-510.5764981
GCGTCGA1201.4874458E-610.3120841
ACGTACT2400.010.3074814
AAAAGTA14500.010.1707772
CCGTTCA750.002662598210.1272279
ATTAACG951.6201689E-410.01536753
TACACCG1054.0403822E-59.9676755
AAAAAGT14800.09.9047471
CTAGACA4150.09.85854
CCCACGT2151.0913936E-119.7402171
CTTACAC4150.09.6292313
TTAACGC1002.715366E-49.5145994
CGGTACT1002.715366E-49.5145994
CACGTAC2550.09.3280373
GTACTTG6650.09.3041371
GTCCAGG6450.09.14991
TTACACT5000.09.1340154
Produced by FastQC (version 0.11.5)