FastQCFastQC Report
Wed 25 May 2016
SRR1294753_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294753_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325592
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92900.1468637243755209No Hit
GGTATCAACGCAGAGTACTTTTTTT64230.10153990330075036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26100.012.1951521
CGTCCGC1158.876614E-710.73482310
TATACCG1356.4726373E-79.8496915
CTAGACT7750.09.5597234
TATACTG12600.09.2717765
GTATCAA50600.09.1445291
TAGACTG8550.09.1091135
GCTAGAC3400.08.9402763
CGCGCTT850.00746454258.93759112
GTATTAG9250.08.9363561
GTACCGT1401.0916403E-58.81816
TCGTCCG1401.0919675E-58.8178919
ACTATCG7200.08.70484111
CGAATTA1752.6147427E-78.68896115
CCGTACA3950.08.66297317
TAGACAG7150.08.6344695
CTAATAC8500.08.6050163
GTCCTAA5750.08.5924781
CTACGCT1000.00291126978.5485984
CTAAGAC6900.08.5353733
Produced by FastQC (version 0.11.5)