##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294753_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6325592
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.084636979432123	33.0	33.0	33.0	27.0	33.0
2	31.070086246473057	33.0	33.0	33.0	27.0	33.0
3	30.973936826782378	33.0	33.0	33.0	27.0	33.0
4	34.480400569622574	37.0	37.0	37.0	27.0	37.0
5	34.639893309590626	37.0	37.0	37.0	27.0	37.0
6	34.89561656837811	37.0	37.0	37.0	33.0	37.0
7	34.82206029095775	37.0	37.0	37.0	33.0	37.0
8	34.748623053779	37.0	37.0	37.0	27.0	37.0
9	34.49516124340615	37.0	37.0	37.0	27.0	37.0
10	34.55648467368746	37.0	37.0	37.0	27.0	37.0
11	34.805595745030665	37.0	37.0	37.0	33.0	37.0
12	34.72206964976559	37.0	37.0	37.0	27.0	37.0
13	34.65972007046929	37.0	37.0	37.0	27.0	37.0
14	35.791049754710706	40.0	37.0	40.0	27.0	40.0
15	36.11992094969135	40.0	37.0	40.0	27.0	40.0
16	36.13709736574853	40.0	37.0	40.0	27.0	40.0
17	36.074390981903356	40.0	37.0	40.0	27.0	40.0
18	36.076335780113546	40.0	37.0	40.0	27.0	40.0
19	36.15949052673647	40.0	37.0	40.0	27.0	40.0
20	36.06202012396626	40.0	37.0	40.0	27.0	40.0
21	36.00862496348168	40.0	37.0	40.0	27.0	40.0
22	35.86259088477411	37.0	37.0	40.0	27.0	40.0
23	35.456697017449116	37.0	37.0	40.0	27.0	40.0
24	35.77675259485594	37.0	37.0	40.0	27.0	40.0
25	34.61317264850468	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.463210426368164
1101	2	0.5573375061089045
1101	3	0.41666820638351254
1101	4	0.56022042525899
1101	5	0.48972963218751175
1101	6	0.4243372075631484
1101	7	0.4582144066563103
1101	8	0.5466920858175897
1101	9	0.7263024200776798
1101	10	0.8126294732193031
1101	11	0.5013717914464237
1101	12	0.6108044769542786
1101	13	0.5338094171023187
1101	14	1.1629719323362977
1101	15	0.9353927184093678
1101	16	0.9600949578420312
1101	17	0.8662655720613088
1101	18	0.9274334961404307
1101	19	0.8676189006974795
1101	20	0.9611568279876366
1101	21	0.9852324192370787
1101	22	1.017028435339391
1101	23	1.3858223353878216
1101	24	1.1626882352910854
1101	25	1.0113827797811723
1102	1	0.30746982908857134
1102	2	0.4417290482199441
1102	3	0.2518219205601504
1102	4	0.26282513370181704
1102	5	0.25082754208148117
1102	6	0.18602223562233178
1102	7	0.2009817122523856
1102	8	0.2981002347788433
1102	9	0.5164976458556367
1102	10	0.5776077856798523
1102	11	0.3318063082183187
1102	12	0.35456756028030156
1102	13	0.42138318625128335
1102	14	1.0027321860286733
1102	15	0.7160429233202379
1102	16	0.5113281335263835
1102	17	0.6631734976118793
1102	18	0.6111208404467661
1102	19	0.6638923492480302
1102	20	0.7060614802006597
1102	21	0.6610509348788511
1102	22	0.8493400819375125
1102	23	1.1281727757395856
1102	24	0.8319371668118762
1102	25	0.7364169907165135
1103	1	0.2828384539619542
1103	2	0.4576275348043133
1103	3	0.15035937670128874
1103	4	-0.0022589048410779355
1103	5	0.2076373269286762
1103	6	0.1662986319645796
1103	7	0.17707453538354656
1103	8	0.3370294986418614
1103	9	0.5433865636860702
1103	10	0.5509266129692989
1103	11	0.36177188585495657
1103	12	0.3092158804018581
1103	13	0.2899441830938372
1103	14	0.8906534336433864
1103	15	0.6839488689526831
1103	16	0.44711426363943474
1103	17	0.3971629328665216
1103	18	0.4906303268253467
1103	19	0.5549081404437928
1103	20	0.6673607194104463
1103	21	0.7213148797611524
1103	22	0.626933590335419
1103	23	1.1572336496371065
1103	24	0.7775425419933057
1103	25	0.6400799107047561
1104	1	0.2816847370903801
1104	2	0.5033422986574543
1104	3	0.30116999159072577
1104	4	0.2875467093021342
1104	5	0.17251341914119678
1104	6	0.1692512313359984
1104	7	0.17310485651297824
1104	8	0.34493144431643685
1104	9	0.48588947001746163
1104	10	0.5798277044878901
1104	11	0.3088925560906901
1104	12	0.33413158113130237
1104	13	0.3405604908584863
1104	14	0.7716191913730768
1104	15	0.6255391162533428
1104	16	0.5268855247703144
1104	17	0.5887084149916859
1104	18	0.5024385175163175
1104	19	0.6088867643038469
1104	20	0.6623239518543755
1104	21	0.7371819521025813
1104	22	0.774103143372713
1104	23	1.1426070832654247
1104	24	0.91589921438365
1104	25	0.714344494931126
1105	1	0.25219893913834923
1105	2	0.3327913801413054
1105	3	0.3401477556365329
1105	4	0.3066280272827697
1105	5	0.31960127748872935
1105	6	0.30030551969859687
1105	7	0.29802620146026015
1105	8	0.3668007398747264
1105	9	-1.5151935814527846
1105	10	-0.09320719836669156
1105	11	0.18333336793509858
1105	12	0.2850022477938481
1105	13	0.28239333983994186
1105	14	-1.5986039778428918
1105	15	-0.19904905345320856
1105	16	0.018628395913786733
1105	17	0.33382938394658623
1105	18	0.2018474273605193
1105	19	0.343685626374473
1105	20	0.4471810233027682
1105	21	0.63231032901259
1105	22	0.686861103972511
1105	23	1.0261257466735287
1105	24	0.7701106017179313
1105	25	0.3311619868140028
1106	1	0.10671844820448939
1106	2	0.0437050752123902
1106	3	0.12679584575266034
1106	4	-1.0270976605006794
1106	5	-0.17463131068321758
1106	6	-0.03887188068507896
1106	7	0.01215459299992716
1106	8	0.12486278691527986
1106	9	0.4213289155215776
1106	10	0.3748589509481519
1106	11	0.2543447005387591
1106	12	0.12070627579598181
1106	13	0.18397972278582841
1106	14	0.6163473650987257
1106	15	0.3912232796855051
1106	16	0.22774843821884616
1106	17	0.11246067686656147
1106	18	0.23504674171898898
1106	19	0.23755808819863944
1106	20	0.336733103745928
1106	21	0.5342471925713994
1106	22	0.53687588464269
1106	23	0.8446761402478842
1106	24	0.5312537801691306
1106	25	0.4466721015017896
1107	1	0.3908784802097287
1107	2	0.3290086189719972
1107	3	0.3175870953490687
1107	4	0.4645741686206861
1107	5	0.5130431489479719
1107	6	0.29831841656543645
1107	7	0.35568240540298746
1107	8	0.39594009653813345
1107	9	0.5251459520575068
1107	10	0.5362909875124515
1107	11	0.2888557860608145
1107	12	0.27310448505425455
1107	13	0.35924100236999124
1107	14	0.8027370893266621
1107	15	0.6503655746179575
1107	16	0.6443759757879874
1107	17	0.5998681798627672
1107	18	0.6550629079541253
1107	19	0.5166859111047373
1107	20	0.6420861437357175
1107	21	0.7394722564637917
1107	22	0.699177509486546
1107	23	0.9541822876263666
1107	24	0.7720266428502143
1107	25	0.741129162347768
1108	1	0.5031883792973488
1108	2	0.4679068503482
1108	3	0.5393495943246336
1108	4	0.6432126417466364
1108	5	0.5863607490209048
1108	6	0.46055211333126067
1108	7	0.46116357067321445
1108	8	0.4761496361516464
1108	9	0.27338239973665424
1108	10	0.573771818655942
1108	11	0.40102130074789244
1108	12	0.386750603194038
1108	13	0.44055486486948325
1108	14	-0.8904590511241111
1108	15	0.05792339959413084
1108	16	0.35796288330692505
1108	17	0.5444493642223733
1108	18	0.6125683860755018
1108	19	0.6133254968516724
1108	20	0.7277858325933408
1108	21	0.7552829616058645
1108	22	0.900711360944932
1108	23	1.1534889446233478
1108	24	1.0238332626747209
1108	25	0.9367321517056268
1109	1	0.41466229489399
1109	2	0.3023218055110348
1109	3	0.46007602510097456
1109	4	0.5670794132141879
1109	5	0.5791127163150733
1109	6	0.38171606569379435
1109	7	0.308584326051232
1109	8	0.4197276972083799
1109	9	0.5950445427931683
1109	10	0.5557953342505968
1109	11	0.3796931483769086
1109	12	0.42941075928550276
1109	13	0.39908920689747873
1109	14	0.8118706426149203
1109	15	0.5780160970213473
1109	16	0.5807963335611603
1109	17	0.5336688985983571
1109	18	0.5695344006079281
1109	19	0.5322437811541576
1109	20	0.6654973772826622
1109	21	0.6412126566700849
1109	22	0.8009855935946035
1109	23	1.1058714391217137
1109	24	0.8971984476347501
1109	25	0.7167229415967
1110	1	0.3690258661276822
1110	2	0.27047607284002595
1110	3	0.36605461191355104
1110	4	0.5773334203597784
1110	5	0.49816933090576043
1110	6	0.31518214040493575
1110	7	0.32110148965775664
1110	8	0.3758523347752174
1110	9	0.3947923084239946
1110	10	0.4113534965645371
1110	11	0.22908408176402162
1110	12	0.20618850638756214
1110	13	0.20122991573968818
1110	14	0.5584097235929875
1110	15	0.4603336077830349
1110	16	0.3902494198388524
1110	17	0.3940905063032645
1110	18	0.4843246010577076
1110	19	0.4100569150691129
1110	20	0.5199001447655505
1110	21	0.5629355107262697
1110	22	0.6835639789774959
1110	23	0.8459170149264068
1110	24	0.6763637504969253
1110	25	0.6021173375902293
1111	1	0.1851079042907564
1111	2	0.06843816572358463
1111	3	0.20049393689199135
1111	4	0.5001337391439336
1111	5	0.3177163299990582
1111	6	0.22755453372521828
1111	7	0.21861516349921573
1111	8	0.2632069047331811
1111	9	0.2620526605768063
1111	10	0.13795598561046063
1111	11	0.08672990612254239
1111	12	0.12846427644355174
1111	13	0.12994069942229203
1111	14	-1.7610595990096627
1111	15	-0.5940305796796324
1111	16	-0.10999567549998801
1111	17	0.04669778090936205
1111	18	0.13449317605331146
1111	19	0.19261416953609256
1111	20	0.2508145790375025
1111	21	0.3534128002230261
1111	22	0.15337352836741047
1111	23	-1.9936414716633806
1111	24	-0.43420480759063906
1111	25	-0.029357935977095906
1112	1	-0.15572860268714805
1112	2	-0.25477304861290406
1112	3	0.021475652017230118
1112	4	0.16245927129014603
1112	5	-0.7704092750927387
1112	6	-0.9792420999328755
1112	7	-1.0840364088745957
1112	8	-0.9124505378819521
1112	9	-0.732248364413941
1112	10	-1.0288897603958205
1112	11	-1.0219869176460676
1112	12	-0.8925288005137304
1112	13	-0.946239516666175
1112	14	-0.6970115807295301
1112	15	-1.028786695795091
1112	16	-0.9515129089424832
1112	17	-1.0167354604404437
1112	18	-1.0469948899542274
1112	19	-0.9580430002176641
1112	20	-1.0245013066973314
1112	21	-2.02214153743018
1112	22	-1.9129375809627618
1112	23	-1.4902335776892528
1112	24	-1.8062337087591658
1112	25	-2.055046050910704
1113	1	0.10169467068187643
1113	2	-0.08570134702179644
1113	3	-0.08553709037634505
1113	4	0.20349967242615463
1113	5	0.12745259712985302
1113	6	-0.04845652641414233
1113	7	0.06115799846416081
1113	8	0.05416553944004221
1113	9	0.2496816825361421
1113	10	0.052890866511972945
1113	11	-0.11897405417713713
1113	12	-0.05342405225182745
1113	13	-0.09704584928238802
1113	14	0.056353586520621946
1113	15	-0.07149807187502688
1113	16	0.045320340360753164
1113	17	0.012328420012721608
1113	18	-0.004061711173257265
1113	19	-0.10847442306839383
1113	20	-0.6842843755165191
1113	21	-0.5260431982855067
1113	22	-0.10015336987935797
1113	23	-0.2911215536536176
1113	24	0.0437323578843376
1113	25	-1.1082800119329903
1114	1	-0.9469208803723923
1114	2	-0.7574848040669941
1114	3	-0.5126429789655482
1114	4	-1.5057813412143588
1114	5	-0.7247621820704566
1114	6	-0.5813629910908489
1114	7	-0.5432713945155427
1114	8	-0.38266549955122997
1114	9	-0.40530582680669625
1114	10	-0.4115621651156829
1114	11	-0.5532278997307998
1114	12	-0.5989423724796907
1114	13	-0.6497938352138846
1114	14	-0.5494454456383977
1114	15	-1.35850708078965
1114	16	-0.7822951549099102
1114	17	-0.791948776540309
1114	18	-0.7462934671538903
1114	19	-0.835438398897459
1114	20	-2.0491899803720983
1114	21	-6.690534956786049
1114	22	-7.840810658834673
1114	23	-7.957974254512969
1114	24	-5.904674900674884
1114	25	-7.359284194968286
1115	1	-1.0550527848334994
1115	2	-1.0171992732583561
1115	3	-0.7763710419527214
1115	4	-0.5860755286175419
1115	5	-0.6497116438819717
1115	6	-0.8639336904392962
1115	7	-0.9915638910809648
1115	8	-0.6163188213999078
1115	9	-2.5549667831008236
1115	10	-1.558652624467122
1115	11	-1.1148995576673144
1115	12	-0.9832728212498054
1115	13	-1.4295207617301955
1115	14	-1.1195815250931531
1115	15	-2.037552865066523
1115	16	-1.331543728206988
1115	17	-3.0984719675083667
1115	18	-2.784052646091169
1115	19	-1.993387037123675
1115	20	-1.7617870215184723
1115	21	-1.2045552009230036
1115	22	-0.9093998710011704
1115	23	-0.24562842140570496
1115	24	-1.2787223215335288
1115	25	-1.0137754897869868
1116	1	-1.5079423528360856
1116	2	-0.7909815955696331
1116	3	-0.5892170325112183
1116	4	-0.2156968352803048
1116	5	-0.21896603976781392
1116	6	-0.3014720686032746
1116	7	-0.6707440865317835
1116	8	-1.6023834098897822
1116	9	-0.7287740924091679
1116	10	-2.1371373617563236
1116	11	-0.96376928333531
1116	12	-0.6425373914681529
1116	13	-0.7043944707732237
1116	14	-0.9669932403548671
1116	15	-1.1039619362592816
1116	16	-0.6646446967343564
1116	17	-0.5194562044516076
1116	18	-1.1149722236401018
1116	19	-0.6148204529313972
1116	20	-0.9768979278787029
1116	21	-0.3559351558441435
1116	22	-0.08911010743565839
1116	23	0.12352862859389546
1116	24	-0.009687017334549353
1116	25	0.039185164460995736
1201	1	0.29863799864578056
1201	2	0.32865050104213367
1201	3	0.260411588428326
1201	4	0.43880921722391975
1201	5	0.37923718625361147
1201	6	0.3205395121586392
1201	7	0.3251732156348197
1201	8	0.42552071527403257
1201	9	0.6930254647930454
1201	10	0.6720062245498752
1201	11	0.46672184652423
1201	12	0.5207709625169343
1201	13	0.4926361921929967
1201	14	1.12928862969612
1201	15	0.8642025722339994
1201	16	0.8204187466638899
1201	17	0.8004659106103489
1201	18	0.6559364921640594
1201	19	0.6665682623557387
1201	20	0.8884024621922677
1201	21	0.8906196396686568
1201	22	0.9053544795687003
1201	23	0.7532430703519211
1201	24	0.8089680846853753
1201	25	0.7212222172588199
1202	1	-0.36241367249756706
1202	2	-0.07350605941860877
1202	3	-0.20910420343296465
1202	4	-0.2238321425274279
1202	5	-0.1831201211545661
1202	6	-0.16395043546371113
1202	7	-0.1677757248663596
1202	8	-0.024317485260546334
1202	9	-0.03180410298228509
1202	10	0.19832268319868263
1202	11	0.05059917644810952
1202	12	0.008064705891186463
1202	13	0.03717588453341136
1202	14	0.18259245877195696
1202	15	0.23171156133362558
1202	16	0.1328551063089094
1202	17	0.017116797241101267
1202	18	-0.06196480820868544
1202	19	-0.03710344823782208
1202	20	0.25337260948968066
1202	21	0.3127320679622443
1202	22	0.21205348437256077
1202	23	0.5213540916244739
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2211	5	0.32211542417803685
2211	6	0.27965994952174356
2211	7	0.19603089449616817
2211	8	0.3271796923185164
2211	9	0.48265414560737696
2211	10	0.40245824770173044
2211	11	0.2045716149083745
2211	12	0.30878198889595154
2211	13	0.14411884649111784
2211	14	0.45179960760648186
2211	15	0.2613507066042331
2211	16	0.224999354131306
2211	17	0.35110976689604456
2211	18	0.2932123590878888
2211	19	0.39414534871418283
2211	20	0.3512802958283814
2211	21	0.40787343696125333
2211	22	0.48942677717802496
2211	23	0.6424246784579637
2211	24	0.5681622921359804
2211	25	0.657942116751407
2212	1	-0.24230156715658424
2212	2	-0.29386427777725643
2212	3	-0.16563599363950132
2212	4	-0.5631289741790297
2212	5	-0.14292228115544958
2212	6	-0.5235667608373831
2212	7	-0.43036927295504057
2212	8	-0.17924933161413747
2212	9	-0.1736120549173492
2212	10	-0.48940336772155035
2212	11	-0.39031212997715414
2212	12	-0.5996932453861135
2212	13	-0.5697458042436025
2212	14	-0.4111517572682928
2212	15	-0.544971303703619
2212	16	-0.400421881865725
2212	17	-0.45085879419553265
2212	18	-0.30169034208812917
2212	19	-0.4310763415264418
2212	20	-0.5270723794784615
2212	21	-0.30863492083543065
2212	22	-0.388041231498633
2212	23	0.07189122261215175
2212	24	-0.2379287455087109
2212	25	-0.8234531385566441
2213	1	-0.16054527073294622
2213	2	-1.3540251625507764
2213	3	-0.29043699623173325
2213	4	0.054146824944339755
2213	5	0.08272659991522602
2213	6	0.04480228339771486
2213	7	0.014958317506447827
2213	8	0.11745057721354613
2213	9	0.07612669602485056
2213	10	-0.41860591486489085
2213	11	-0.10954880985550375
2213	12	0.02340292038570624
2213	13	-0.3187286531228821
2213	14	-0.020799659098862833
2213	15	-0.09022944426299517
2213	16	-0.056718829832277606
2213	17	0.1075792740234931
2213	18	0.08524689785991058
2213	19	0.08642555030743893
2213	20	0.15887287608546075
2213	21	-0.38295420057080776
2213	22	-0.8888214228323505
2213	23	-0.11606117972976193
2213	24	0.036789363466205316
2213	25	-0.5539226328465929
2214	1	-0.3034162476837423
2214	2	-0.5479122317205345
2214	3	-0.5030506767730252
2214	4	-0.6257613805156765
2214	5	-0.33912132244508797
2214	6	-0.32046686148267156
2214	7	-0.5777994304254719
2214	8	-0.13671149371468516
2214	9	-0.11430879715515374
2214	10	-2.092997869775182
2214	11	-1.0494891744318622
2214	12	-0.6169927657277299
2214	13	-0.4147748885219187
2214	14	-0.8958316156337034
2214	15	-0.664684066098701
2214	16	-0.8741458292344433
2214	17	-0.6181701714511618
2214	18	-0.6401485327784471
2214	19	-1.4873636225124471
2214	20	-2.455395191440516
2214	21	-1.923478914225413
2214	22	-2.1382129628780433
2214	23	-2.620012245606624
2214	24	-1.6672854733759408
2214	25	-1.9173017937225438
2215	1	-0.20402533420109492
2215	2	-0.24576337824532501
2215	3	-0.7080265813811266
2215	4	-0.1640641660159261
2215	5	-0.0920545485863542
2215	6	-0.1091141546060399
2215	7	-0.09865688009011109
2215	8	-0.1091389982556521
2215	9	-0.1415028232582003
2215	10	-0.174331165705631
2215	11	-0.4374801177925036
2215	12	-0.323783920193101
2215	13	-0.1877494678978664
2215	14	-0.036935886241288074
2215	15	-0.5426399345031072
2215	16	-0.3140265867031715
2215	17	-0.1062340016938208
2215	18	-0.1029682859167309
2215	19	-2.060188829647551
2215	20	-2.2719201149030823
2215	21	-3.1271531185931067
2215	22	-2.2358204677052953
2215	23	-3.483040790169433
2215	24	-2.436104981977344
2215	25	-1.7265806611843928
2216	1	-1.4318250454133619
2216	2	-1.1019991535706168
2216	3	-0.7035616885940215
2216	4	-0.7623100131266156
2216	5	-1.7702728141422455
2216	6	-1.513388043113217
2216	7	-0.9916204971296665
2216	8	-5.019033064687569
2216	9	-3.947231386047168
2216	10	-1.9096381502893038
2216	11	-0.9521963936832023
2216	12	-1.0054845918840343
2216	13	-1.8103710396618027
2216	14	-3.183092674363415
2216	15	-1.8591022221170874
2216	16	-2.7523740284088447
2216	17	-2.4659337984330563
2216	18	-2.4072359119885007
2216	19	-1.4669002816737162
2216	20	-0.7756292659315562
2216	21	-0.6059423840631126
2216	22	-0.5203451399115337
2216	23	-0.8807367507196702
2216	24	-0.3984618853282811
2216	25	-0.2971984772763463
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	6.0
7	175.0
8	1000.0
9	2424.0
10	4471.0
11	7540.0
12	11955.0
13	17269.0
14	21675.0
15	26443.0
16	29961.0
17	31833.0
18	33551.0
19	34874.0
20	36238.0
21	39301.0
22	41841.0
23	45428.0
24	48242.0
25	52725.0
26	57166.0
27	64812.0
28	73448.0
29	87149.0
30	104531.0
31	128856.0
32	171557.0
33	244185.0
34	352573.0
35	557147.0
36	960681.0
37	3036535.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53910933535241	19.824747670739924	13.461540286181373	24.17460270772629
2	15.91061737639539	19.715138778300663	39.88167779857083	24.492566046733128
3	18.81269822898182	24.10148185428562	29.05502636517599	28.03079355155657
4	12.606359193488506	16.46049443387151	37.30855661411401	33.62458975852597
5	13.582678379233485	37.57928901449588	35.04297343231998	13.795059173950655
6	32.67264353074503	37.444664521547324	16.741622524765	13.141069422942659
7	27.94035138917718	32.00796322360372	21.768710816827994	18.282974570391104
8	25.3924751946824	36.883981320860336	19.704234835213775	18.01930864924349
9	24.686295854934894	17.036157827324313	21.014502147324514	37.26304417041628
10	14.876575783856726	29.99485264755085	33.429192760444806	21.699378808147614
11	33.888497089804936	24.297366177426333	22.923376588268383	18.89076014450035
12	21.71585092353082	26.125344207589784	30.763566181922464	21.395238686956933
13	27.72332544451664	22.519441427512206	26.13417944449451	23.623053683476645
14	21.054081010457782	22.343396101733152	27.3555935005594	29.246929387249665
15	22.88773264338022	30.60885432097048	23.575446126080436	22.927966909568863
16	22.64644607785015	29.85292392585236	25.114830065415273	22.385799930882214
17	21.401900230545117	29.160424020894666	27.42723315979608	22.01044258876414
18	21.7709742160433	28.910361247311457	27.963582008854715	21.355082527790533
19	22.199336216242877	28.223836204702383	28.17468635948725	21.40214121956749
20	22.750891896895187	27.959851162655404	28.04558266197017	21.243674278479244
21	22.9421277394246	27.387545406892748	27.758031159978298	21.912295693704355
22	22.651509494143525	27.34679760931365	28.04605881027661	21.95563408626621
23	22.904033731775428	27.34903369222128	27.80509299179647	21.94183958420682
24	22.5835560556818	27.498555848584004	27.84045691780937	22.07743117792483
25	22.587350395499186	27.622262667611626	27.75616341944272	22.03422351744647
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2761.0
1	2761.0
2	3158.0
3	3555.0
4	3555.0
5	3555.0
6	5714.0
7	7873.0
8	7873.0
9	7873.0
10	13109.5
11	18346.0
12	18346.0
13	18346.0
14	32315.5
15	46285.0
16	46285.0
17	46285.0
18	75627.5
19	104970.0
20	104970.0
21	104970.0
22	155674.0
23	206378.0
24	206378.0
25	206378.0
26	271823.5
27	337269.0
28	337269.0
29	337269.0
30	405628.0
31	473987.0
32	473987.0
33	473987.0
34	531757.0
35	589527.0
36	589527.0
37	589527.0
38	629904.5
39	670282.0
40	670282.0
41	670282.0
42	688511.5
43	706741.0
44	706741.0
45	706741.0
46	708022.5
47	709304.0
48	709304.0
49	709304.0
50	692111.0
51	674918.0
52	674918.0
53	674918.0
54	635697.5
55	596477.0
56	596477.0
57	596477.0
58	535653.0
59	474829.0
60	474829.0
61	474829.0
62	406647.5
63	338466.0
64	338466.0
65	338466.0
66	268471.5
67	198477.0
68	198477.0
69	198477.0
70	148744.5
71	99012.0
72	99012.0
73	99012.0
74	71272.5
75	43533.0
76	43533.0
77	43533.0
78	29305.5
79	15078.0
80	15078.0
81	15078.0
82	10065.0
83	5052.0
84	5052.0
85	5052.0
86	3374.5
87	1697.0
88	1697.0
89	1697.0
90	1153.5
91	610.0
92	610.0
93	610.0
94	368.5
95	127.0
96	127.0
97	127.0
98	82.5
99	38.0
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030194802320478463
2	0.013089683937882811
3	0.018195925377419223
4	0.020045554629511356
5	0.017437103120150652
6	0.008473515206165684
7	2.0551436134357066E-4
8	5.21690301872141E-4
9	7.272046632157117E-4
10	1.5808797026428515E-4
11	0.0011224245888764245
12	0.0030669066231271314
13	0.0028772010588099897
14	9.959542126649964E-4
15	0.002181613989647135
16	0.0024977899301757054
17	0.0023555107569378487
18	0.0023238931628849916
19	6.007342870042835E-4
20	4.5845511376642686E-4
21	0.08285390521551184
22	0.080055748141834
23	0.08128883430989542
24	0.0012330861680614241
25	0.22450072657231135
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	6325592.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.41090060203395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.56940897412123	35.76625969710795
2	15.124269101691624	15.551045909309627
3	6.36444614675907	9.816057247140856
4	3.1233624612249504	6.4229950815264
5	1.7577032986882144	4.518255478836349
6	1.1039050859507173	3.4051652788735245
7	0.6819073051881509	2.4540228080780198
8	0.477384377461993	1.9634208622929903
9	0.3252480879972539	1.504916741072557
>10	1.3652636917356586	11.554026545189577
>50	0.0684271739072832	2.4371958579630584
>100	0.036690941043913544	3.355600968128467
>500	0.0013015976358646709	0.42583965198604684
>1k	5.88789786072054E-4	0.4803245556016744
>5k	9.296680799592833E-5	0.3448733168929702
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9290	0.1468637243755209	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6423	0.10153990330075036	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	1.5808797026428514E-5	0.0	0.0	0.0	0.0
4	1.5808797026428514E-5	0.0	0.0	3.161759405285703E-5	0.0
5	1.5808797026428514E-5	0.0	0.0	3.161759405285703E-5	0.0
6	1.5808797026428514E-5	0.0	0.0	3.161759405285703E-5	0.0
7	1.5808797026428514E-5	0.0	0.0	3.161759405285703E-5	0.0
8	1.5808797026428514E-5	0.0	0.0	3.161759405285703E-5	0.0
9	1.5808797026428514E-5	0.0	0.0	7.904398513214258E-5	0.0
10	1.5808797026428514E-5	0.0	0.0	9.485278215857108E-5	0.0
11	1.5808797026428514E-5	0.0	0.0	1.8970556431714217E-4	0.0
12	1.5808797026428514E-5	1.5808797026428514E-5	0.0	2.371319553964277E-4	0.0
13	1.5808797026428514E-5	1.5808797026428514E-5	0.0	2.371319553964277E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2610	0.0	12.195152	1
CGTCCGC	115	8.876614E-7	10.734823	10
TATACCG	135	6.4726373E-7	9.849691	5
CTAGACT	775	0.0	9.559723	4
TATACTG	1260	0.0	9.271776	5
GTATCAA	5060	0.0	9.144529	1
TAGACTG	855	0.0	9.109113	5
GCTAGAC	340	0.0	8.940276	3
CGCGCTT	85	0.0074645425	8.937591	12
GTATTAG	925	0.0	8.936356	1
GTACCGT	140	1.0916403E-5	8.8181	6
TCGTCCG	140	1.0919675E-5	8.817891	9
ACTATCG	720	0.0	8.704841	11
CGAATTA	175	2.6147427E-7	8.688961	15
CCGTACA	395	0.0	8.662973	17
TAGACAG	715	0.0	8.634469	5
CTAATAC	850	0.0	8.605016	3
GTCCTAA	575	0.0	8.592478	1
CTACGCT	100	0.0029112697	8.548598	4
CTAAGAC	690	0.0	8.535373	3
>>END_MODULE