FastQCFastQC Report
Wed 25 May 2016
SRR1294753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6325592
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT245920.38876993647393004No Hit
GGTATCAACGCAGAGTACTTTTTTT178970.28293004038199115No Hit
TATCAACGCAGAGTACTTTTTTTTT169320.2676745512514876No Hit
ACGCAGAGTACTTTTTTTTTTTTTT92770.14665821001417734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.013.0379261
CTGATCG1751.8189894E-1211.3933289
TTCGTCG600.00590506211.07675812
TGGACCG2100.010.4200865
TAGACAG8150.010.0393045
TGATCGG1901.0186341E-109.99406710
CGAACCG1356.505834E-79.84600712
GCGTCAA1554.109097E-89.80063411
GTGGTAT15050.09.6762471
GTATCAA83600.09.5864511
TCGCTCG900.0011204939.49443915
CGACATC800.0045408589.49436410
TAACGCT1651.0437361E-79.2256894
TCGTCCG1356.9072776E-69.1427939
GATCGGT1356.9079706E-69.14272111
CGCCCTA3950.09.1338916
CTTAACG1252.709017E-59.1336492
CGAATTA1701.703138E-78.93594315
GTTCTAG7550.08.8247641
TGGTATC14250.08.813172
Produced by FastQC (version 0.11.5)