FastQCFastQC Report
Wed 25 May 2016
SRR1294752_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294752_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624639
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC172190.47505420539811No Hit
CCCATGTACTCTGCGTTGATACCAC146660.40461960487651316No Hit
GAGTACATGGGAAGCAGTGGTATCA77580.21403510804800147No Hit
GTATCAACGCAGAGTACTTTTTTTT52360.14445576511205668No Hit
CATGTACTCTGCGTTGATACCACTG50080.13816548351435826No Hit
ATTCCATTCCATTCCATTCCATTCC47950.13228903623229787No Hit
GCGTTGATACCACTGCTTCCCATGT47860.13204073564291505No Hit
GTATCAACGCAGAGTACATGGGAAG45910.12666088953962035No Hit
ACGCAGAGTACATGGGAAGCAGTGG45440.12536420868395445No Hit
GAATGGAATGGAATGGAATGGAATG39930.11016269482284995No Hit
GGTATCAACGCAGAGTACTTTTTTT36630.10105833987881276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG456.7770755E-414.7744185
AACAGCG802.8812015E-511.8700077
ATACCGT951.6519598E-49.99593456
CTTAGAC2950.09.981473
AGACCGT1054.126708E-59.9483346
GTGTTAC2600.09.8672271
GTATTAG5000.09.691811
AACGATT1106.7966146E-59.50571117
CGCCCTA800.00449607249.50557916
GAACGAT1201.6900385E-59.50557816
TATACCG1106.8584784E-59.497845
GTGTAGG4700.09.0974441
AACACCG1054.505347E-49.0455635
GTCTTAG4000.09.0266861
GATCGCC850.00746528948.93729411
GACCGTC850.00746528948.93729411
GTATAGG3950.08.9003961
TACACTG8650.08.893935
CCTACAC4700.08.8922093
GTCTAGG4300.08.8388591
Produced by FastQC (version 0.11.5)