##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294752_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3624639
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.184435194787675	33.0	33.0	33.0	27.0	33.0
2	31.175317597145536	33.0	33.0	33.0	27.0	33.0
3	31.14082947294889	33.0	33.0	33.0	27.0	33.0
4	34.5615817188967	37.0	37.0	37.0	27.0	37.0
5	34.90225288642538	37.0	37.0	37.0	33.0	37.0
6	35.109059412537356	37.0	37.0	37.0	33.0	37.0
7	35.04031711847718	37.0	37.0	37.0	33.0	37.0
8	34.95678907609834	37.0	37.0	37.0	33.0	37.0
9	34.658685182165726	37.0	37.0	37.0	27.0	37.0
10	34.75135206568157	37.0	37.0	37.0	27.0	37.0
11	34.99558659496849	37.0	37.0	37.0	33.0	37.0
12	34.92792137368715	37.0	37.0	37.0	33.0	37.0
13	34.85411264404538	37.0	37.0	37.0	33.0	37.0
14	36.07843125894744	40.0	37.0	40.0	27.0	40.0
15	36.39172287226397	40.0	37.0	40.0	27.0	40.0
16	36.423929941712814	40.0	37.0	40.0	27.0	40.0
17	36.36953252448037	40.0	37.0	40.0	27.0	40.0
18	36.36867450799928	40.0	37.0	40.0	27.0	40.0
19	36.449097965342204	40.0	37.0	40.0	27.0	40.0
20	36.347365903197534	40.0	37.0	40.0	27.0	40.0
21	36.30148905863453	40.0	37.0	40.0	27.0	40.0
22	36.1652120390472	40.0	37.0	40.0	27.0	40.0
23	35.797924703673935	37.0	37.0	40.0	27.0	40.0
24	36.11198577292801	40.0	37.0	40.0	27.0	40.0
25	35.06713992759003	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.46720132391211777
1101	2	0.6242002837435017
1101	3	0.44646297716188954
1101	4	0.5498621228909641
1101	5	0.5291365399963226
1101	6	0.3688399303191474
1101	7	0.41061494765567375
1101	8	0.5578137369395293
1101	9	0.7071450458968798
1101	10	0.7492743730555205
1101	11	0.5265878480053701
1101	12	0.5371206303994072
1101	13	0.5725105495541811
1101	14	1.2583907614859342
1101	15	1.038309010199356
1101	16	0.9376499484775636
1101	17	0.929674404170278
1101	18	0.8888435745907515
1101	19	0.9636713295789434
1101	20	1.0561493274902247
1101	21	0.9792109625368042
1101	22	1.1077645619120986
1101	23	1.4638290972128019
1101	24	1.2150012312598122
1101	25	1.095742506394764
1102	1	0.23811629399871492
1102	2	0.5207131989570364
1102	3	0.27917017741019023
1102	4	0.1405369833299801
1102	5	0.18922258391393143
1102	6	0.1500939398564256
1102	7	0.25815816801009106
1102	8	0.3074767853569611
1102	9	0.5559404254335121
1102	10	0.6010618152429572
1102	11	0.49229934844352385
1102	12	0.422528165484934
1102	13	0.37429302884220306
1102	14	1.1064009437346698
1102	15	0.7320214459253904
1102	16	0.635489534569345
1102	17	0.7525348829853584
1102	18	0.7676390993634072
1102	19	0.7550533048899482
1102	20	0.8631733427077535
1102	21	0.8388460044743127
1102	22	0.9388369742655698
1102	23	1.2055803932838174
1102	24	0.9808792395812773
1102	25	0.7465429133444914
1103	1	0.39113569822731975
1103	2	0.5440373305829524
1103	3	0.3699948811736711
1103	4	0.12090672652040979
1103	5	0.4134865327141668
1103	6	0.25100271299756827
1103	7	0.3181848116192896
1103	8	0.3835145647714171
1103	9	0.6177719966930724
1103	10	0.6035802020698284
1103	11	0.37633722271365855
1103	12	0.4297755946742541
1103	13	0.4035348041462967
1103	14	1.1298057576524485
1103	15	0.7541298465695476
1103	16	0.5451530862713625
1103	17	0.5785602655951152
1103	18	0.5684455532243433
1103	19	0.7003771732285387
1103	20	0.8355451347179894
1103	21	0.8313795748840178
1103	22	0.7640041014494869
1103	23	1.3068013735222266
1103	24	0.927210046479189
1103	25	0.8234602671553475
1104	1	0.27072657116447374
1104	2	0.4893224422761584
1104	3	0.38635779453040087
1104	4	0.31347616976080417
1104	5	0.2843764583451005
1104	6	0.1856847366966221
1104	7	0.23409694559184402
1104	8	0.42536642604714103
1104	9	0.5153757310059959
1104	10	0.5748209682341567
1104	11	0.324343988682692
1104	12	0.29950633471637644
1104	13	0.3894407991788569
1104	14	0.846686386530898
1104	15	0.6655004108964278
1104	16	0.5208554109548587
1104	17	0.6253663617824543
1104	18	0.5725210918339485
1104	19	0.6618769600281595
1104	20	0.7442451963475776
1104	21	0.7673365601218123
1104	22	0.8007839253154216
1104	23	1.2687165276521029
1104	24	0.9471854321744715
1104	25	0.7524970405242826
1105	1	0.2003102596705908
1105	2	0.3459197654255135
1105	3	0.3433802363700629
1105	4	0.23619514398914276
1105	5	0.3382096209414769
1105	6	0.2858453854752838
1105	7	0.3319302010865428
1105	8	0.29150593102246347
1105	9	-1.4860617616682816
1105	10	-0.09347515678085472
1105	11	0.11071177677000321
1105	12	0.29250771621603633
1105	13	0.3067023961373039
1105	14	-1.5317836289025593
1105	15	-0.17927287909402878
1105	16	0.0684461328800623
1105	17	0.25893941633372464
1105	18	0.24248862459603515
1105	19	0.30556413799122595
1105	20	0.46916560679849084
1105	21	0.5200187633071423
1105	22	0.5867230384485254
1105	23	0.951088454069378
1105	24	0.6778657200576674
1105	25	0.30448040156381495
1106	1	-0.039991146827997426
1106	2	-0.020423139415044034
1106	3	0.030600971008638567
1106	4	-1.2342950645292348
1106	5	-0.27847806710771295
1106	6	-0.13221181943638527
1106	7	-0.03479286000271742
1106	8	0.022769878248851683
1106	9	0.37144012447210173
1106	10	0.3322495205870126
1106	11	0.21586460023268472
1106	12	0.15312965993657457
1106	13	0.19310280882879738
1106	14	0.5267833165188449
1106	15	0.26972436661682764
1106	16	0.2070073014881757
1106	17	0.036329699357814604
1106	18	0.1391835499294558
1106	19	0.09388734744119631
1106	20	0.24409857515868794
1106	21	0.41966557516052205
1106	22	0.41181078419354833
1106	23	0.6079282118480975
1106	24	0.31517930666702654
1106	25	0.2879099849933979
1107	1	0.3865655766387661
1107	2	0.310359553271919
1107	3	0.3273746990676152
1107	4	0.5254541564993218
1107	5	0.4693391686377595
1107	6	0.22269381763794627
1107	7	0.2918482803916973
1107	8	0.35569169385357213
1107	9	0.43895713994400154
1107	10	0.4482481514536687
1107	11	0.2788295178391138
1107	12	0.2666514494537111
1107	13	0.32775097850056767
1107	14	0.7260157615639429
1107	15	0.5096017110019702
1107	16	0.4723241426652436
1107	17	0.5512357202223015
1107	18	0.5834163884862633
1107	19	0.5469750871969765
1107	20	0.5837424669227644
1107	21	0.6217326571151958
1107	22	0.5787767057409212
1107	23	0.858917549329071
1107	24	0.7623252731381385
1107	25	0.5929858803424395
1108	1	0.4935350799630811
1108	2	0.37439622723530874
1108	3	0.5730431196670338
1108	4	0.7333427749713621
1108	5	0.58305474147582
1108	6	0.5355555379423222
1108	7	0.4827278300796607
1108	8	0.5644527465757889
1108	9	0.33661040511415763
1108	10	0.6162676577238599
1108	11	0.41970158951921377
1108	12	0.43070761155878756
1108	13	0.44621621936040157
1108	14	-0.8470058521448323
1108	15	0.17635448806215948
1108	16	0.5532112610035824
1108	17	0.6189116913435981
1108	18	0.6776596820728571
1108	19	0.657647337442846
1108	20	0.7451835923402825
1108	21	0.7999403825771267
1108	22	0.869222530973083
1108	23	1.2080362774332372
1108	24	1.0445611521666294
1108	25	0.9537589703912559
1109	1	0.4020858939224503
1109	2	0.22121519434310954
1109	3	0.41815614123872535
1109	4	0.589739036596086
1109	5	0.4641798356988218
1109	6	0.30416433664617415
1109	7	0.31113388522153684
1109	8	0.38098843743581057
1109	9	0.44597164094161457
1109	10	0.4221540841507192
1109	11	0.2755053670655343
1109	12	0.25417352487022526
1109	13	0.31662861375402684
1109	14	0.6892523952448855
1109	15	0.480498143633433
1109	16	0.4581455006937887
1109	17	0.374445357693844
1109	18	0.4244811336285821
1109	19	0.4532776601675863
1109	20	0.5386267180935818
1109	21	0.5374798357993242
1109	22	0.7409458815172059
1109	23	1.144772144171995
1109	24	0.8723640035309401
1109	25	0.6929203140967175
1110	1	0.32120756298775177
1110	2	0.17781376798480508
1110	3	0.3816083353358408
1110	4	0.5073889851933231
1110	5	0.4216825563964832
1110	6	0.2655038390392832
1110	7	0.2802507035952573
1110	8	0.3028086451862535
1110	9	0.34106514515527664
1110	10	0.4336353493713574
1110	11	0.21620884969210152
1110	12	0.16045245675643116
1110	13	0.14954104790391654
1110	14	0.5330895703380989
1110	15	0.38966994541583944
1110	16	0.32302599580187774
1110	17	0.3656833331086702
1110	18	0.4803020910203202
1110	19	0.3712228999271332
1110	20	0.47370436983432285
1110	21	0.5440274802148579
1110	22	0.595185392033251
1110	23	0.7311426843131983
1110	24	0.5937367577487649
1110	25	0.6314168838260414
1111	1	0.11438151180154676
1111	2	-0.03139139310454908
1111	3	0.16028177503777385
1111	4	0.4972497195959562
1111	5	0.3157083613755418
1111	6	0.24807219496577204
1111	7	0.09565953421886064
1111	8	0.2422221118381458
1111	9	0.33469949292381784
1111	10	0.22148484636322507
1111	11	0.10163903635397986
1111	12	0.11351804916667163
1111	13	0.08126077492453732
1111	14	-1.8828609604525326
1111	15	-0.5622273440136709
1111	16	-0.12205651446338095
1111	17	0.09849484432199063
1111	18	0.2089008009584603
1111	19	0.24608714455900582
1111	20	0.3567454491047002
1111	21	0.3633072097050416
1111	22	0.11184097376546731
1111	23	-1.8596888237477032
1111	24	-0.3879132908705998
1111	25	-0.04224972016631057
1112	1	-0.29613160954200524
1112	2	-0.3871514114344663
1112	3	-0.11079821505029841
1112	4	0.04196039498268078
1112	5	-0.798943013513508
1112	6	-1.0273393726960194
1112	7	-1.1383052560617202
1112	8	-0.9084542790470849
1112	9	-0.8563894306498696
1112	10	-1.0939018889146368
1112	11	-0.9760510901148365
1112	12	-0.9195271254875053
1112	13	-1.1183168609681005
1112	14	-0.7467619236676839
1112	15	-1.0996661576324627
1112	16	-1.0775044590460254
1112	17	-0.9734901909434157
1112	18	-1.1549907412257312
1112	19	-1.1104991289373771
1112	20	-1.0155017927417163
1112	21	-1.9521176569859762
1112	22	-1.917147901925901
1112	23	-1.44644128311203
1112	24	-1.864687262613991
1112	25	-2.1417204035952935
1113	1	0.036965328216322035
1113	2	-0.08042533692566423
1113	3	-0.02191971860339237
1113	4	0.2772986620858262
1113	5	0.07395698895915359
1113	6	-0.037553120459293154
1113	7	-0.01794746701435912
1113	8	0.07898713004504287
1113	9	0.2831534630518746
1113	10	-0.12980799349334404
1113	11	-0.07297983744552994
1113	12	-0.07518859658278387
1113	13	-0.13830520460302154
1113	14	-0.018843271922719396
1113	15	-0.19664969502817087
1113	16	-0.04114968203086278
1113	17	-0.07450628248782465
1113	18	-0.037119048026063695
1113	19	-0.16528128251250962
1113	20	-0.5721114350100649
1113	21	-0.5161728748586825
1113	22	-0.08016607232821116
1113	23	-0.2022972361424351
1113	24	0.018330182617802393
1113	25	-0.9832793625057477
1114	1	-0.8578628172524851
1114	2	-0.6972841756950956
1114	3	-0.46983794976478777
1114	4	-1.557501437207442
1114	5	-0.7364817370832171
1114	6	-0.6491379953414338
1114	7	-0.4393126764063382
1114	8	-0.3986056549224557
1114	9	-0.46910425929411304
1114	10	-0.48892268764505786
1114	11	-0.5784798186715889
1114	12	-0.5368192068608835
1114	13	-0.5875877273194874
1114	14	-0.6413693364049351
1114	15	-1.3053471905850103
1114	16	-0.7754403818397861
1114	17	-0.7791481905038822
1114	18	-0.7525909100668358
1114	19	-0.8435455486184651
1114	20	-1.9652657797162263
1114	21	-6.593715804594094
1114	22	-7.702973067914247
1114	23	-7.980309931495675
1114	24	-5.89091989247795
1114	25	-7.363272447439037
1115	1	-0.8695625912566243
1115	2	-0.8267726752515507
1115	3	-0.6637109802214276
1115	4	-0.4008540963549905
1115	5	-0.6457940337925479
1115	6	-0.8328245792133515
1115	7	-0.8682467468663546
1115	8	-0.5694484927545886
1115	9	-2.579374662284863
1115	10	-1.4113836663972208
1115	11	-1.0235208464247236
1115	12	-0.9073528844682883
1115	13	-1.2727337797843177
1115	14	-0.9385552127197272
1115	15	-1.7430048068567103
1115	16	-1.2010956642290083
1115	17	-2.8340047253748324
1115	18	-2.59202217141943
1115	19	-2.018104645479866
1115	20	-1.7516033267106934
1115	21	-1.2539695747119524
1115	22	-0.8382263100043943
1115	23	-0.31683202417509904
1115	24	-1.2753871653204243
1115	25	-0.890486203616959
1116	1	-1.493674951674734
1116	2	-0.7861216384475505
1116	3	-0.6455333139016872
1116	4	-0.32825567698965585
1116	5	-0.41435577600459794
1116	6	-0.4525379450649112
1116	7	-0.8659442874622627
1116	8	-1.7264246655786266
1116	9	-0.8270676463374897
1116	10	-2.474929796867734
1116	11	-1.145142576645327
1116	12	-0.8140000275944317
1116	13	-0.7517726038967538
1116	14	-1.1871234995461108
1116	15	-1.2575126831109742
1116	16	-0.8148398533231926
1116	17	-0.7654336599762246
1116	18	-1.276242717569076
1116	19	-0.925743828180444
1116	20	-1.1883101303520434
1116	21	-0.6178637095346815
1116	22	-0.31200751346232636
1116	23	-0.08223438795743476
1116	24	-0.19086678023867876
1116	25	-0.2907044974948292
1201	1	0.25647558688069694
1201	2	0.3678148484151258
1201	3	0.3394880165574463
1201	4	0.3481022750007483
1201	5	0.3990597860702465
1201	6	0.362617649511229
1201	7	0.3537101900307107
1201	8	0.4172334189390057
1201	9	0.7287655263928343
1201	10	0.695232019588012
1201	11	0.509544961274834
1201	12	0.4736175516542289
1201	13	0.5120477491443509
1201	14	1.1749658147623023
1201	15	0.9690141756606891
1201	16	0.8449471038088916
1201	17	0.7655346453170182
1201	18	0.603256296209473
1201	19	0.717722151343267
1201	20	0.93567805878358
1201	21	0.8952633381537041
1201	22	1.0821666182702145
1201	23	0.73380507041783
1201	24	0.8476339728109892
1201	25	0.7231392441032511
1202	1	-0.1338025853937772
1202	2	0.18989022854618653
1202	3	0.046075962524795955
1202	4	-0.07954393440702745
1202	5	0.11558059316583069
1202	6	0.03404400069963032
1202	7	0.003996162919108315
1202	8	0.16366225620092933
1202	9	0.278556725990299
1202	10	0.49932680597921575
1202	11	0.29617828180563066
1202	12	0.21232235200913152
1202	13	0.27103581301410884
1202	14	0.4885109556050722
1202	15	0.503043014113544
1202	16	0.41184594294653465
1202	17	0.2499075146626879
1202	18	0.26238697435674396
1202	19	0.36116930813858517
1202	20	0.4764638962033061
1202	21	0.435684869039072
1202	22	0.46532550963028996
1202	23	0.7557979188626263
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2211	4	0.4662377301292864
2211	5	0.3472561858533112
2211	6	0.2754540647471444
2211	7	0.2301364212439836
2211	8	0.3847482588757032
2211	9	0.49960936593083716
2211	10	0.4360785088936012
2211	11	0.31926757766029823
2211	12	0.2632113447593838
2211	13	0.19092642693508566
2211	14	0.5382560831812881
2211	15	0.2977468190638817
2211	16	0.33596518413894927
2211	17	0.4935154481223023
2211	18	0.4002457640024204
2211	19	0.45753468647745166
2211	20	0.580507965992183
2211	21	0.42743245771270466
2211	22	0.489291868078908
2211	23	0.6202514844403026
2211	24	0.5639912605386073
2211	25	0.5949831108818486
2212	1	-0.2788074006421475
2212	2	-0.36636032142985187
2212	3	-0.2906434001726126
2212	4	-0.6362906986197245
2212	5	-0.2134476241545542
2212	6	-0.5464786965265986
2212	7	-0.5012338944677097
2212	8	-0.3269927262576857
2212	9	-0.2900054692440932
2212	10	-0.6036192425547
2212	11	-0.5122950478185402
2212	12	-0.6399378698323588
2212	13	-0.6247531322133639
2212	14	-0.5376321794494103
2212	15	-0.5982759116881553
2212	16	-0.5480447034115983
2212	17	-0.49611143010552183
2212	18	-0.5006641717494986
2212	19	-0.5845830187640146
2212	20	-0.5383846680042765
2212	21	-0.46161326302318884
2212	22	-0.60961314722568
2212	23	-0.07547069486821556
2212	24	-0.3658852500553422
2212	25	-0.8954890555233987
2213	1	-0.17223171115464098
2213	2	-1.298413001367912
2213	3	-0.37649145660606465
2213	4	0.05854453200376497
2213	5	0.007955982247210613
2213	6	-0.08568333483741952
2213	7	-0.10873106103395003
2213	8	0.024221369031174333
2213	9	0.07316285306419701
2213	10	-0.5109857233615216
2213	11	-0.16816036047123362
2213	12	-0.08319628094718468
2213	13	-0.4215328931852511
2213	14	-0.14948005317981483
2213	15	-0.20419085308210327
2213	16	-0.31967527784507155
2213	17	-0.1053930243935639
2213	18	-0.12181610050983949
2213	19	-0.03865409807250586
2213	20	0.0709956529776079
2213	21	-0.594093786570788
2213	22	-1.0385324139886123
2213	23	-0.21753664731681255
2213	24	-0.07781944678630026
2213	25	-0.6813210486153238
2214	1	-0.22764403241413333
2214	2	-0.48947540837545844
2214	3	-0.43182930985397405
2214	4	-0.42546807202189996
2214	5	-0.3515757010902263
2214	6	-0.27775919298436946
2214	7	-0.46691958037193615
2214	8	-0.18333053149515877
2214	9	-0.1437130743920605
2214	10	-2.031307024322871
2214	11	-0.9950857670710178
2214	12	-0.6597020305589254
2214	13	-0.4430523713710883
2214	14	-0.8097072484928489
2214	15	-0.6503633157641744
2214	16	-0.8455212068119167
2214	17	-0.6049375279780023
2214	18	-0.5307791167876488
2214	19	-1.4909197324056933
2214	20	-2.553899519165185
2214	21	-2.0114833631225224
2214	22	-2.141621738084659
2214	23	-2.652982729711951
2214	24	-1.8190224963957675
2214	25	-2.0903299008551883
2215	1	-0.31056289495256095
2215	2	-0.38261585790177577
2215	3	-0.8309976234244623
2215	4	-0.27478970449570284
2215	5	-0.23969172154320262
2215	6	-0.27015343823187266
2215	7	-0.13374740834106547
2215	8	-0.1403135997554017
2215	9	-0.22444371150857023
2215	10	-0.26902661149897966
2215	11	-0.6155209025876758
2215	12	-0.4394415844832622
2215	13	-0.2869078516396115
2215	14	-0.13900919654479793
2215	15	-0.61127424963707
2215	16	-0.4511591075585102
2215	17	-0.3002971804028718
2215	18	-0.35511859071951335
2215	19	-2.2245308827288
2215	20	-2.2743226950231445
2215	21	-3.065326138704428
2215	22	-2.1688554494216277
2215	23	-3.3954687310294744
2215	24	-2.283885607318666
2215	25	-1.6066436595602482
2216	1	-1.467737539524748
2216	2	-1.1705686439590686
2216	3	-0.9091330467443122
2216	4	-0.9162378510668034
2216	5	-1.8692716247911392
2216	6	-1.5591519567898189
2216	7	-1.1968927670806906
2216	8	-5.345528881968637
2216	9	-4.14443940911697
2216	10	-2.0748888048611747
2216	11	-1.1226449090456967
2216	12	-1.245137152576369
2216	13	-1.935543801300149
2216	14	-3.5220218464631827
2216	15	-2.1736798483643867
2216	16	-3.0409773721718594
2216	17	-2.7201074020573515
2216	18	-2.689255850317842
2216	19	-1.688845944943452
2216	20	-1.0415789815417256
2216	21	-0.8805852875701348
2216	22	-0.8277764066520135
2216	23	-1.2013654470184036
2216	24	-0.7520961820353875
2216	25	-0.502211752669325
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	48.0
8	274.0
9	777.0
10	1650.0
11	2990.0
12	5237.0
13	7913.0
14	10486.0
15	13915.0
16	15893.0
17	16903.0
18	17833.0
19	18466.0
20	19139.0
21	20445.0
22	21709.0
23	23483.0
24	24985.0
25	27276.0
26	30106.0
27	33796.0
28	39093.0
29	46202.0
30	55779.0
31	68355.0
32	92372.0
33	132525.0
34	193958.0
35	309577.0
36	545088.0
37	1828359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.892380889301016	20.880276637082172	13.17362674967987	23.053715723936946
2	15.294589651697585	20.590674850417702	40.64894047943595	23.46579501844876
3	18.863323542819423	25.8425967930824	28.65140913862242	26.642670525475754
4	12.312461970851562	17.016693502655315	37.497009408463065	33.17383511803006
5	13.55068762447042	37.944199171434384	34.48796074233695	14.017152461758245
6	32.15066880306917	37.89012132739136	16.82604608349353	13.133163786045928
7	26.951570504402177	32.41914622652962	22.733826486356442	17.89545678271176
8	24.241375837861476	37.64252439264958	20.640492419069375	17.47560735041957
9	24.032042907809732	17.349466066494415	21.907896642723234	36.710594382972616
10	14.909839532328181	30.607767547970216	32.985938007384554	21.496454912317052
11	32.91924420526284	25.895344621807425	21.99966782376561	19.185743349164124
12	21.022778691328867	27.677130811932326	31.312588979263	19.987501517475803
13	26.797133888968133	23.76129205093138	26.32844290470327	23.113131155397216
14	20.301959586612668	23.05358039017368	27.977197979027153	28.667262044186504
15	23.661635704245075	30.260128484738125	23.371282819546124	22.70695299147068
16	22.026164816604048	31.171407745266876	25.571807021096614	21.23062041703246
17	20.59292146000578	30.161059504861417	28.319457170123496	20.926561865009308
18	20.82963520473125	29.75670843057446	29.053620266728764	20.36003609796553
19	21.21024933613822	28.521129772045384	29.330620409007828	20.938000482808565
20	22.50639308572846	28.24798965077433	28.276296074152896	20.969321189344313
21	22.516335573998273	28.812296633700985	27.77238106177917	20.898986730521568
22	21.202650307127918	28.49248595517252	28.819025087681805	21.485838650017755
23	21.100904765099873	28.93448011833434	28.635054698154384	21.329560418411404
24	21.280846835045615	28.359637830581125	28.551520966991685	21.80799436738158
25	22.01462367351634	27.971660756435814	27.467088162356955	22.546627407690895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2048.0
1	2048.0
2	2658.0
3	3268.0
4	3268.0
5	3268.0
6	4819.0
7	6370.0
8	6370.0
9	6370.0
10	9905.5
11	13441.0
12	13441.0
13	13441.0
14	22509.5
15	31578.0
16	31578.0
17	31578.0
18	49581.0
19	67584.0
20	67584.0
21	67584.0
22	96121.5
23	124659.0
24	124659.0
25	124659.0
26	162352.5
27	200046.0
28	200046.0
29	200046.0
30	241108.5
31	282171.0
32	282171.0
33	282171.0
34	318545.0
35	354919.0
36	354919.0
37	354919.0
38	384620.5
39	414322.0
40	414322.0
41	414322.0
42	415166.0
43	416010.0
44	416010.0
45	416010.0
46	436847.0
47	457684.0
48	457684.0
49	457684.0
50	428558.0
51	399432.0
52	399432.0
53	399432.0
54	356648.0
55	313864.0
56	313864.0
57	313864.0
58	273393.0
59	232922.0
60	232922.0
61	232922.0
62	194489.0
63	156056.0
64	156056.0
65	156056.0
66	120826.0
67	85596.0
68	85596.0
69	85596.0
70	62233.5
71	38871.0
72	38871.0
73	38871.0
74	27419.0
75	15967.0
76	15967.0
77	15967.0
78	10654.5
79	5342.0
80	5342.0
81	5342.0
82	3535.0
83	1728.0
84	1728.0
85	1728.0
86	1131.0
87	534.0
88	534.0
89	534.0
90	344.5
91	155.0
92	155.0
93	155.0
94	107.0
95	59.0
96	59.0
97	59.0
98	36.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030872039946598817
2	0.015091158043601031
3	0.019946814013754196
4	0.02135385068692358
5	0.021684918139434024
6	0.013187520191665985
7	0.0019588157606867883
8	0.0023174721675731018
9	0.0034210303426079123
10	0.001269091901290032
11	0.0015725703994246046
12	0.005490201920798182
13	0.0020967605325661397
14	0.0013242698100417725
15	0.0023174721675731018
16	0.00212434948694201
17	0.00215193844131788
18	0.0016001593538004751
19	3.8624536126218365E-4
20	5.517790875174052E-4
21	0.08538781379331845
22	0.08334623116950406
23	0.08376006548514212
24	0.0010207913119071996
25	0.22929179981785774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3624639.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.57800061949229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50463986431063	64.51343085389514
2	9.881572188980233	14.738957936626509
3	1.9911400772305028	4.454857377395771
4	0.6034187488550716	1.8000705530370715
5	0.278281896297774	1.0376853717244436
6	0.15736093931919307	0.7041398538018402
7	0.10719934989551436	0.5596299228041788
8	0.07533032288652719	0.44943878935183823
9	0.052505100062559734	0.35241528464928756
>10	0.30951592969714253	4.310263406800678
>50	0.022853350753248694	1.189306509610411
>100	0.01383984102603503	1.871638159246872
>500	9.667009302234106E-4	0.5571805137656339
>1k	0.001189785734343394	2.077683791169317
>5k	1.1154241259469318E-4	0.49917206433597205
>10k+	7.43616083964621E-5	0.8841296117849389
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	17219	0.47505420539811	No Hit
CCCATGTACTCTGCGTTGATACCAC	14666	0.40461960487651316	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7758	0.21403510804800147	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5236	0.14445576511205668	No Hit
CATGTACTCTGCGTTGATACCACTG	5008	0.13816548351435826	No Hit
ATTCCATTCCATTCCATTCCATTCC	4795	0.13228903623229787	No Hit
GCGTTGATACCACTGCTTCCCATGT	4786	0.13204073564291505	No Hit
GTATCAACGCAGAGTACATGGGAAG	4591	0.12666088953962035	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4544	0.12536420868395445	No Hit
GAATGGAATGGAATGGAATGGAATG	3993	0.11016269482284995	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3663	0.10105833987881276	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.7588954375870258E-5	0.0
3	0.0	0.0	0.0	8.276686312761078E-5	0.0
4	0.0	0.0	0.0	1.1035581750348103E-4	0.0
5	0.0	0.0	0.0	1.1035581750348103E-4	0.0
6	0.0	0.0	0.0	1.1035581750348103E-4	0.0
7	0.0	0.0	0.0	1.379447718793513E-4	0.0
8	8.276686312761078E-5	0.0	0.0	1.379447718793513E-4	0.0
9	8.276686312761078E-5	0.0	0.0	1.9312268063109182E-4	0.0
10	8.276686312761078E-5	0.0	0.0	2.758895437587026E-4	0.0
11	8.276686312761078E-5	0.0	0.0	3.0347849813457285E-4	0.0
12	8.276686312761078E-5	0.0	0.0	3.310674525104431E-4	0.0
13	8.276686312761078E-5	0.0	0.0	3.310674525104431E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	45	6.7770755E-4	14.774418	5
AACAGCG	80	2.8812015E-5	11.870007	7
ATACCGT	95	1.6519598E-4	9.9959345	6
CTTAGAC	295	0.0	9.98147	3
AGACCGT	105	4.126708E-5	9.948334	6
GTGTTAC	260	0.0	9.867227	1
GTATTAG	500	0.0	9.69181	1
AACGATT	110	6.7966146E-5	9.505711	17
CGCCCTA	80	0.0044960724	9.505579	16
GAACGAT	120	1.6900385E-5	9.505578	16
TATACCG	110	6.8584784E-5	9.49784	5
GTGTAGG	470	0.0	9.097444	1
AACACCG	105	4.505347E-4	9.045563	5
GTCTTAG	400	0.0	9.026686	1
GATCGCC	85	0.0074652894	8.937294	11
GACCGTC	85	0.0074652894	8.937294	11
GTATAGG	395	0.0	8.900396	1
TACACTG	865	0.0	8.89393	5
CCTACAC	470	0.0	8.892209	3
GTCTAGG	430	0.0	8.838859	1
>>END_MODULE