FastQCFastQC Report
Wed 25 May 2016
SRR1294752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624639
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC166490.4593285014038639No Hit
CCCATGTACTCTGCGTTGATACCAC161130.4445408218583975No Hit
GTATCAACGCAGAGTACTTTTTTTT132680.36605024665904656No Hit
GGTATCAACGCAGAGTACTTTTTTT101330.27955887469069335No Hit
TATCAACGCAGAGTACTTTTTTTTT92490.25517023902242403No Hit
GAGTACATGGGAAGCAGTGGTATCA82860.22860207595846097No Hit
CATGTACTCTGCGTTGATACCACTG57240.15791917484748136No Hit
ACGCAGAGTACATGGGAAGCAGTGG56440.15571205849741174No Hit
GCGTTGATACCACTGCTTCCCATGT54770.1511047031166414No Hit
ATTCCATTCCATTCCATTCCATTCC54270.1497252553978479No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51590.14233141562511467No Hit
GTATCAACGCAGAGTACATGGGAAG50810.14017947718379678No Hit
GAATGGAATGGAATGGAATGGAATG44070.12158452193446023No Hit
GCTTCCCATGTACTCTGCGTTGATA41120.11344578039357851No Hit
TATCAACGCAGAGTACATGGGAAGC40110.11065929600161561No Hit
GGTATCAACGCAGAGTACATGGGAA39040.10770727788339748No Hit
ACTCTGCGTTGATACCACTGCTTCC38540.10632783016460397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTG350.00214850416.3110735
CCGTTAT658.068347E-411.6853139
AGGACCG752.0454935E-411.4177515
GTCTACG1002.3505756E-510.4700351
AGCACCG1105.9399263E-610.3797745
GACTGCG1201.5370897E-610.2855097
TAGGCCG750.002615053210.1491135
TATACTG8950.09.7805685
TCGGAAC1304.2757238E-69.49418513
GTATTAG4500.09.3066991
CTACACT4950.09.2264654
CCTACAC4350.09.1866973
AGACCGT1151.11729794E-49.0825226
TCTAGAC3150.09.06170753
TCCGAAT1054.5227143E-49.04208113
CAATCCG1054.5227143E-49.04208110
TAGACAG6000.09.0390535
CGGGACT950.0017990279.0140144
TATCGGT2950.09.01143113
ACTGCGG950.00183464128.9946168
Produced by FastQC (version 0.11.5)