FastQCFastQC Report
Wed 25 May 2016
SRR1294751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4769803
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1481363.1057047848726667No Hit
CCCATGTACTCTGCGTTGATACCAC1465883.073250614333548No Hit
GAGTACATGGGAAGCAGTGGTATCA657141.377708890702614No Hit
CATGTACTCTGCGTTGATACCACTG523081.0966490649613831No Hit
GCGTTGATACCACTGCTTCCCATGT463710.9721785155487553No Hit
ACGCAGAGTACATGGGAAGCAGTGG381710.8002636586877906No Hit
GCTTCCCATGTACTCTGCGTTGATA376410.7891520886711674No Hit
GTATCAACGCAGAGTACATGGGAAG372430.7808079285454766No Hit
ACTCTGCGTTGATACCACTGCTTCC309980.6498800893873394No Hit
TATCAACGCAGAGTACATGGGAAGC300800.6306340115094901No Hit
GGTATCAACGCAGAGTACATGGGAA246460.5167089709994312No Hit
CAGTGGTATCAACGCAGAGTACATG198820.41683063220850003No Hit
GTGGTATCAACGCAGAGTACATGGG191510.40150505167613837No Hit
GTACATGGTAAGCAGTGGTATCAAC176930.37093775151720104No Hit
GCAGTGGTATCAACGCAGAGTACAT165210.3463665061219509No Hit
ATACCACTGCTTCCCATGTACTCTG160420.33632416265409704No Hit
GTACTCTGCGTTGATACCACTGCTT152670.32007611215809123No Hit
ACATGGGAAGCAGTGGTATCAACGC141870.2974336675959154No Hit
CATGGGAAGCAGTGGTATCAACGCA140870.29533714495126945No Hit
CTGCTTCCCATGTACTCTGCGTTGA138190.28971846426361847No Hit
GGGAAGCAGTGGTATCAACGCAGAG137290.2878315938834371No Hit
ACCATGTACTCTGCGTTGATACCAC128760.26994825572460746No Hit
TACCACTGCTTCCCATGTACTCTGC124090.2601574949741111No Hit
CTTCCCATGTACTCTGCGTTGATAC122720.2572852589509462No Hit
GATACCACTGCTTCCCATGTACTCT120250.25210684801867084No Hit
CCATGTACTCTGCGTTGATACCACT113530.23801821584665028No Hit
GCAGAGTACATGGGAAGCAGTGGTA111110.23294463104660715No Hit
GTTGATACCACTGCTTCCCATGTAC110100.23082714317551478No Hit
AAGCAGTGGTATCAACGCAGAGTAC109120.22877255098376179No Hit
CCACTGCTTCCCATGTACTCTGCGT108870.22824842032260034No Hit
GTACATGGGTAAGCAGTGGTATCAA99220.20801697680176726No Hit
ATGGGAAGCAGTGGTATCAACGCAG92540.1940122055355326No Hit
TTCCCATGTACTCTGCGTTGATACC90800.19036425613384872No Hit
GGTAAGCAGTGGTATCAACGCAGAG81950.1718100307287324No Hit
TGATACCACTGCTTCCCATGTACTC81370.17059404759483776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA76060.15946151235176798No Hit
GCTTACCATGTACTCTGCGTTGATA64750.13574984124082273No Hit
CCCCATGTACTCTGCGTTGATACCA64190.13457578855982102No Hit
ATCAACGCAGAGTACATGGGAAGCA63820.13380007518130205No Hit
GAGTACATGGTAAGCAGTGGTATCA56080.1175729899117427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCAC400.005285361314.2483183
ACGACGT400.005288671214.2469735
ACACCGT1157.122435E-811.5609426
TGCTACG600.005882013611.0824892
TAGAACG600.005885687711.0815594
TACACCG1201.2811688E-711.0809785
CCGTGCG600.005896260511.0788889
GTACAAA10250.010.936441
GTACTAG2300.010.7389891
CGCAAAA2600.010.5953472
GAACAAA18800.010.2578431
TAGACAG5200.010.2285965
ATCGCTT800.0044973799.50535716
GTCTACG800.0045188349.4998741
TCCGTAC900.00111560719.49887853
TGTTCGG800.00453331749.49618910
CTAGGAC3150.09.3481023
GTGCTAC2750.09.3271491
GTCCTGG6350.09.2754681
ACTAGAC2750.08.9807583
Produced by FastQC (version 0.11.5)