##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294751_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4769803
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89824149970135	33.0	33.0	33.0	27.0	33.0
2	30.88485771844246	33.0	33.0	33.0	27.0	33.0
3	30.91535373683148	33.0	33.0	33.0	27.0	33.0
4	34.25040090754272	37.0	37.0	37.0	27.0	37.0
5	34.63243052176369	37.0	37.0	37.0	27.0	37.0
6	34.67375717613495	37.0	37.0	37.0	27.0	37.0
7	34.72234702355632	37.0	37.0	37.0	27.0	37.0
8	34.62656235488132	37.0	37.0	37.0	27.0	37.0
9	34.35193193513443	37.0	37.0	37.0	27.0	37.0
10	34.47587101605664	37.0	37.0	37.0	27.0	37.0
11	34.71097988742931	37.0	37.0	37.0	27.0	37.0
12	34.64977421499378	37.0	37.0	37.0	27.0	37.0
13	34.51418559634433	37.0	37.0	37.0	27.0	37.0
14	35.73071466473563	40.0	37.0	40.0	27.0	40.0
15	35.96479120835808	40.0	37.0	40.0	27.0	40.0
16	35.949814698846055	40.0	37.0	40.0	27.0	40.0
17	35.94308905420203	40.0	37.0	40.0	27.0	40.0
18	35.935184115570394	40.0	37.0	40.0	27.0	40.0
19	35.939060376288076	40.0	37.0	40.0	27.0	40.0
20	35.87728277247509	40.0	37.0	40.0	27.0	40.0
21	35.80141653649008	40.0	37.0	40.0	27.0	40.0
22	35.718077455190496	40.0	37.0	40.0	27.0	40.0
23	35.37814832184893	37.0	33.0	40.0	27.0	40.0
24	35.706097086189935	37.0	37.0	40.0	27.0	40.0
25	34.59634286782913	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4282249311668238
1101	2	0.5677191220063023
1101	3	0.42727005002256035
1101	4	0.5682396679263135
1101	5	0.5023915366414826
1101	6	0.37061481704533605
1101	7	0.39394126041836586
1101	8	0.48910616774151805
1101	9	0.780637611529869
1101	10	0.7560039626235096
1101	11	0.5238498056463712
1101	12	0.5368380322978012
1101	13	0.5659144619734349
1101	14	1.2092189114949363
1101	15	0.9467973940161585
1101	16	0.9477609415199169
1101	17	1.027675056080625
1101	18	0.9104984518630204
1101	19	0.9194740646510908
1101	20	1.0033129123755629
1101	21	1.037230785171353
1101	22	1.0009273967397405
1101	23	1.3615999506295822
1101	24	1.156730743313652
1101	25	0.9105747007828668
1102	1	0.1978703985367254
1102	2	0.4330208039792538
1102	3	0.1950600323940499
1102	4	0.09661494425432693
1102	5	0.1349840497471817
1102	6	0.10807542850049856
1102	7	0.1760192556065121
1102	8	0.256009620765667
1102	9	0.45580622393365644
1102	10	0.4626048337053703
1102	11	0.2779824555133814
1102	12	0.3035371846253554
1102	13	0.28714398171644717
1102	14	0.9428251362885689
1102	15	0.6948021500734285
1102	16	0.4239784647906717
1102	17	0.5583838516902659
1102	18	0.5504304148611823
1102	19	0.5470769580270058
1102	20	0.6004406363990284
1102	21	0.6547016016664102
1102	22	0.6482237969644373
1102	23	0.9593182263699802
1102	24	0.7119974098442157
1102	25	0.6923468084702407
1103	1	0.12113881874959631
1103	2	0.36268876480867007
1103	3	0.14786960099907986
1103	4	-0.01622834681927543
1103	5	0.08643989973953126
1103	6	-0.006082856024733019
1103	7	0.02252717681326999
1103	8	0.24400357432207187
1103	9	0.3783211655301315
1103	10	0.3546566190516316
1103	11	0.14881035956805277
1103	12	0.2068773544177347
1103	13	0.0611055490716339
1103	14	0.7043454561949574
1103	15	0.5519229260392322
1103	16	0.3077232875856666
1103	17	0.3393663904652868
1103	18	0.4265225308837586
1103	19	0.5108238915925796
1103	20	0.5189450307906966
1103	21	0.6945349172714899
1103	22	0.4913596375164957
1103	23	0.8740888836672625
1103	24	0.5964121114444154
1103	25	0.5010450114898077
1104	1	0.19690044116644145
1104	2	0.36599098614828307
1104	3	0.2525434924569936
1104	4	0.1584886664293279
1104	5	0.08019210344946259
1104	6	0.017916609005688144
1104	7	0.038507974647451704
1104	8	0.214847124088422
1104	9	0.4066293647495627
1104	10	0.46320215023185085
1104	11	0.23989379455912285
1104	12	0.23165515966448424
1104	13	0.2283775689465699
1104	14	0.6892884940926933
1104	15	0.5052930317767803
1104	16	0.3757023183991066
1104	17	0.43665709374765527
1104	18	0.4102866092973301
1104	19	0.48551557484195484
1104	20	0.5096612821129227
1104	21	0.563198871264099
1104	22	0.6234365353413622
1104	23	0.9714608455871598
1104	24	0.6843779301302533
1104	25	0.5682377991271466
1105	1	0.24041340363513442
1105	2	0.26608516867318244
1105	3	0.25941798206978817
1105	4	0.2810663183212583
1105	5	0.1754200364878571
1105	6	0.2682815638054308
1105	7	0.16661385655381622
1105	8	0.3066725420022607
1105	9	-1.6922562645950023
1105	10	-0.1639746456106863
1105	11	-0.0336143445082655
1105	12	0.14674205144044095
1105	13	0.23199372875199487
1105	14	-1.499974439707863
1105	15	-0.2172405000043085
1105	16	-0.04855696555534195
1105	17	0.09609878753062873
1105	18	0.09872219165000473
1105	19	0.2454212055021756
1105	20	0.38724136955421073
1105	21	0.5279060617888547
1105	22	0.5294630244994423
1105	23	0.9124500979679055
1105	24	0.6429498416380284
1105	25	0.32531594677410425
1106	1	0.07978138613677288
1106	2	0.1022155431834264
1106	3	0.21311338122157153
1106	4	-1.034160878899037
1106	5	-0.226260464370732
1106	6	-0.022773988759027475
1106	7	0.06359629122277255
1106	8	0.20307849729250194
1106	9	0.36460990245620195
1106	10	0.36362195189158797
1106	11	0.2768173934610658
1106	12	0.14204945677109748
1106	13	0.2048959150401899
1106	14	0.660931372666262
1106	15	0.4517857050761549
1106	16	0.24936425068045054
1106	17	0.00975141252532552
1106	18	0.26131498316275525
1106	19	0.2488549127810913
1106	20	0.3825654083065828
1106	21	0.47062799868952965
1106	22	0.5353639497116376
1106	23	0.6613830420791729
1106	24	0.4373342671524725
1106	25	0.28198894727266577
1107	1	0.3082295754425779
1107	2	0.2933737562217935
1107	3	0.24294000428623974
1107	4	0.41446939963778817
1107	5	0.36979730062601135
1107	6	0.19584862205709186
1107	7	0.19398252732643328
1107	8	0.211949010066256
1107	9	0.4204941239259128
1107	10	0.4226699298510539
1107	11	0.25405268262598923
1107	12	0.2220273856220345
1107	13	0.321195472131393
1107	14	0.7358458593454529
1107	15	0.567264055133947
1107	16	0.5324678450539722
1107	17	0.4595867794030255
1107	18	0.5633600088471411
1107	19	0.5591220043016136
1107	20	0.6270335507956162
1107	21	0.6723067477569558
1107	22	0.5785337575323481
1107	23	0.8684368048228706
1107	24	0.7725052870403673
1107	25	0.6946740948121288
1108	1	0.4884732014411526
1108	2	0.397616247753092
1108	3	0.4921087161712485
1108	4	0.6488372833446974
1108	5	0.571519487337163
1108	6	0.4006710420515702
1108	7	0.4043315691962164
1108	8	0.4659004653113783
1108	9	0.2640479870268777
1108	10	0.48794871393310046
1108	11	0.37166845019266503
1108	12	0.4184174699874248
1108	13	0.4303687779295515
1108	14	-0.9596616587292814
1108	15	-0.012850202534060884
1108	16	0.4351655802165766
1108	17	0.49818932121262094
1108	18	0.6377997011059122
1108	19	0.6823491391285117
1108	20	0.693579269890563
1108	21	0.7278432728692508
1108	22	0.8970685837034722
1108	23	1.1272626694030592
1108	24	0.9326214170888747
1108	25	0.8874902888230167
1109	1	0.38707203326507056
1109	2	0.3043369243884264
1109	3	0.43078445666293774
1109	4	0.5360895773151313
1109	5	0.5062651326264884
1109	6	0.4168559303262427
1109	7	0.22378863746158828
1109	8	0.3852572966288932
1109	9	0.5059712508929977
1109	10	0.4504157438459728
1109	11	0.33349590620610314
1109	12	0.31011970802166644
1109	13	0.3100197893421637
1109	14	0.7408375534806879
1109	15	0.5140626501761574
1109	16	0.4488985931678755
1109	17	0.4856236853447058
1109	18	0.4764794954485083
1109	19	0.44104010590560705
1109	20	0.6922743508002043
1109	21	0.6537342055890036
1109	22	0.7268141579211118
1109	23	1.0384579734706776
1109	24	0.839305122175908
1109	25	0.6875458727725743
1110	1	0.29041117903836167
1110	2	0.14105514957200072
1110	3	0.25217703062640595
1110	4	0.45130262379977637
1110	5	0.42825114125148644
1110	6	0.22903282901245348
1110	7	0.24717059576430245
1110	8	0.21740643240522672
1110	9	0.3516542774470679
1110	10	0.32336512234721226
1110	11	0.15493881328649195
1110	12	0.14365524760042092
1110	13	0.12764527872221265
1110	14	0.5009104575083612
1110	15	0.41393836379455706
1110	16	0.3312164231740269
1110	17	0.28870093353732074
1110	18	0.4169653435564271
1110	19	0.3235580016949058
1110	20	0.42733208276215606
1110	21	0.5095886912637155
1110	22	0.5926411100965936
1110	23	0.7384796161831702
1110	24	0.6487625723427826
1110	25	0.6337584543840009
1111	1	0.17810550957331728
1111	2	0.14621062495645631
1111	3	0.25781055152578247
1111	4	0.5293367234211459
1111	5	0.2845210153038522
1111	6	0.28875701845790047
1111	7	0.21103878448880664
1111	8	0.24260684721807735
1111	9	0.2081412612695317
1111	10	0.16210494890044203
1111	11	0.13229728757283254
1111	12	0.13535245092801773
1111	13	0.1530643783011243
1111	14	-1.7841813086044596
1111	15	-0.5914366523320354
1111	16	-0.14969983004478848
1111	17	0.0956144399631711
1111	18	0.1625526157264119
1111	19	0.1830997244337098
1111	20	0.2722012800319078
1111	21	0.2738005304125295
1111	22	0.06216161695494549
1111	23	-1.8676323474040188
1111	24	-0.4096507753508618
1111	25	0.0400564318426575
1112	1	0.014802804804844527
1112	2	-0.14147464913358831
1112	3	0.12317334132062285
1112	4	0.2963649559769337
1112	5	-0.5780694501480568
1112	6	-0.7835185876879791
1112	7	-0.8810673860242346
1112	8	-0.6495953205945924
1112	9	-0.512937673633914
1112	10	-0.7397901465858183
1112	11	-0.7500066383084203
1112	12	-0.6426890711625006
1112	13	-0.7204756378300914
1112	14	-0.45983887780467825
1112	15	-0.7907254769921934
1112	16	-0.7181647703161289
1112	17	-0.663427250834161
1112	18	-0.7854133367525264
1112	19	-0.6209278663446156
1112	20	-0.7389286147015568
1112	21	-1.5623102809009737
1112	22	-1.4441556783257923
1112	23	-1.1702706173299902
1112	24	-1.416902508186574
1112	25	-1.622634785554851
1113	1	0.09966742099450343
1113	2	-0.005752784134735123
1113	3	0.056820660128273914
1113	4	0.32247778710030417
1113	5	0.1499074915376113
1113	6	0.04074497003956168
1113	7	0.14612429135331695
1113	8	0.23027667635806637
1113	9	0.2659574171259038
1113	10	0.007785237371926712
1113	11	-0.11316345202983058
1113	12	0.09859002059344846
1113	13	-0.031062043378142334
1113	14	0.11741815098776698
1113	15	-0.048516395521410516
1113	16	0.09209609034426336
1113	17	0.06999493094304654
1113	18	0.13084123488877708
1113	19	0.021956858957366876
1113	20	-0.5710644044490181
1113	21	-0.491944485592974
1113	22	0.017192824216216707
1113	23	-0.11507228514387435
1113	24	0.14769075659322795
1113	25	-0.9642829445534176
1114	1	-0.7706856914436067
1114	2	-0.568914639245719
1114	3	-0.4864683862589665
1114	4	-1.3924847455186082
1114	5	-0.6297025296735583
1114	6	-0.5435131455267523
1114	7	-0.474651671975316
1114	8	-0.36574838329537585
1114	9	-0.3384868001574546
1114	10	-0.37292083606357096
1114	11	-0.4658675680939979
1114	12	-0.4348282141945674
1114	13	-0.5267130444486412
1114	14	-0.5988121297738687
1114	15	-1.276407706515343
1114	16	-0.8190625352933267
1114	17	-0.6130394489403272
1114	18	-0.7285497462483335
1114	19	-0.7307181994182983
1114	20	-1.9449267770140182
1114	21	-6.596923288624438
1114	22	-7.6415059781716295
1114	23	-7.662695186388433
1114	24	-5.773533944524306
1114	25	-7.072648151426176
1115	1	-1.0999429518721868
1115	2	-1.0518335979566409
1115	3	-0.8075183537070387
1115	4	-0.7223685703105502
1115	5	-0.763287831298392
1115	6	-0.9180988110522534
1115	7	-1.1679860909081654
1115	8	-0.8614734658153935
1115	9	-2.810215636667774
1115	10	-1.6104135251289193
1115	11	-1.2222096372916553
1115	12	-1.1533459083426436
1115	13	-1.472443795853735
1115	14	-1.138894776658212
1115	15	-2.0264217582843145
1115	16	-1.4693516805507159
1115	17	-3.1927361362879836
1115	18	-2.748021740189536
1115	19	-2.1445090711964667
1115	20	-1.8427321637074598
1115	21	-1.2582927435153692
1115	22	-0.8766616412593464
1115	23	-0.34951787105035237
1115	24	-1.3650475111459102
1115	25	-1.0805830809762256
1116	1	-1.3939860067967942
1116	2	-0.7679042564601843
1116	3	-0.5404746243022096
1116	4	-0.1499741839509241
1116	5	-0.24785663509655365
1116	6	-0.3346299572162579
1116	7	-0.6674241118516235
1116	8	-1.6803110604199247
1116	9	-0.8186132545798301
1116	10	-2.426881220239096
1116	11	-0.9959859541528928
1116	12	-0.6571368717562862
1116	13	-0.7914647769100185
1116	14	-0.9976844761210728
1116	15	-1.1151583467919153
1116	16	-0.6994771820731529
1116	17	-0.49332286099212297
1116	18	-1.1874227572369662
1116	19	-0.7634380495416551
1116	20	-0.9982413725717194
1116	21	-0.40347610988350624
1116	22	-0.022445244324408975
1116	23	0.10654642259343206
1116	24	0.04883638409295088
1116	25	0.06472019445066479
1201	1	0.25345112971744044
1201	2	0.37493224485748655
1201	3	0.27099038923000407
1201	4	0.34950443467281644
1201	5	0.3705829208050133
1201	6	0.21503021173500514
1201	7	0.23337637889859764
1201	8	0.32727140967174506
1201	9	0.6285053060639356
1201	10	0.5596071888011807
1201	11	0.3034282990512338
1201	12	0.3572757313942816
1201	13	0.3912693603578532
1201	14	1.0302758591982482
1201	15	0.7488683156444509
1201	16	0.6516443285227353
1201	17	0.6193890909056989
1201	18	0.45954976477954546
1201	19	0.5700240368488707
1201	20	0.6597070791033346
1201	21	0.6322778401728968
1201	22	0.8093263147954985
1201	23	0.5619995311521393
1201	24	0.6431031367282927
1201	25	0.6211652458896779
1202	1	-0.35785000287634006
1202	2	-0.07173775614045397
1202	3	-0.12156280835660027
1202	4	-0.11831336856590013
1202	5	-0.08617621371182338
1202	6	-0.18847773064882745
1202	7	-0.1956270184781701
1202	8	0.007840703751000433
1202	9	0.007210956760872023
1202	10	0.2434232837429775
1202	11	-0.015169034764888067
1202	12	0.024360580179489943
1202	13	0.06414807329923633
1202	14	0.2516859913847611
1202	15	0.2554164078945078
1202	16	0.25662489736167515
1202	17	0.058183167123999624
1202	18	0.05469974562989677
1202	19	0.0699040565514295
1202	20	0.17015472066238857
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2211	1	0.3126481496434437
2211	2	0.18980545961393247
2211	3	0.27248892879015685
2211	4	0.4327624512048587
2211	5	0.3731841454091267
2211	6	0.26678682902209516
2211	7	0.22383769724859093
2211	8	0.28954548868848207
2211	9	0.504584233952805
2211	10	0.4347930536028173
2211	11	0.2522848811512546
2211	12	0.2951209088830282
2211	13	0.2698477174865843
2211	14	0.5105562436434496
2211	15	0.4069080458455474
2211	16	0.3567195482359651
2211	17	0.3998739339878554
2211	18	0.3076560767982244
2211	19	0.33435710363133353
2211	20	0.45475130712966205
2211	21	0.3905204697177993
2211	22	0.4994682869659357
2211	23	0.6588976116328524
2211	24	0.5509360399320826
2211	25	0.6475866592800656
2212	1	-0.23013730614086825
2212	2	-0.30328374400306046
2212	3	-0.2441606873897939
2212	4	-0.4413714370994697
2212	5	-0.17381048573695068
2212	6	-0.4930061781154862
2212	7	-0.4672838320348731
2212	8	-0.2339898101262179
2212	9	-0.12548532175129878
2212	10	-0.4359101508003107
2212	11	-0.4592573558661144
2212	12	-0.6035922181947626
2212	13	-0.5489317182231233
2212	14	-0.3685144934836231
2212	15	-0.6610186686940125
2212	16	-0.39565837289588757
2212	17	-0.4848999512708687
2212	18	-0.2859191386346609
2212	19	-0.41920901388599674
2212	20	-0.4006854129634121
2212	21	-0.3275088950968055
2212	22	-0.4313562234410213
2212	23	0.05520680070818429
2212	24	-0.17360337809309812
2212	25	-0.8161334572699559
2213	1	-0.08312873139367127
2213	2	-1.1551117366239936
2213	3	-0.26702581447369766
2213	4	0.06049980504319308
2213	5	0.06807134673093884
2213	6	0.04716678580908251
2213	7	0.09792151778748348
2213	8	0.12522742819026433
2213	9	0.08918470421208013
2213	10	-0.46375276490252304
2213	11	-0.10628813613394783
2213	12	0.05283938880168648
2213	13	-0.36731744519770615
2213	14	-0.04521256672767748
2213	15	-0.16061432324443814
2213	16	0.0358815970412536
2213	17	0.032647192757551124
2213	18	-0.013385420405640502
2213	19	0.024590009071090435
2213	20	0.17041888465889343
2213	21	-0.39911336353080884
2213	22	-0.7846135000193186
2213	23	-0.015233107736747797
2213	24	0.08329642376533286
2213	25	-0.5097132263325435
2214	1	-0.11864348256390755
2214	2	-0.3264867982155195
2214	3	-0.3493703574645828
2214	4	-0.3321084420620082
2214	5	-0.18735994792292132
2214	6	-0.1115774553833333
2214	7	-0.371661230426362
2214	8	0.007088508505610491
2214	9	0.08760115530841261
2214	10	-1.7517729361269616
2214	11	-0.6465109434756613
2214	12	-0.466890409178113
2214	13	-0.1784474341112272
2214	14	-0.585557910408383
2214	15	-0.4374508593370692
2214	16	-0.5860954293199185
2214	17	-0.41751417703796534
2214	18	-0.3671304006963467
2214	19	-1.2271002163538896
2214	20	-2.3081277093297743
2214	21	-1.8018724790765077
2214	22	-1.8843379703322043
2214	23	-2.442951850037808
2214	24	-1.5417211606150616
2214	25	-1.9018151295106662
2215	1	-0.156446776808842
2215	2	-0.33742161473313814
2215	3	-0.7364669108726574
2215	4	-0.16454543997598847
2215	5	-0.06607874298568106
2215	6	-0.057338179541744694
2215	7	-0.1088995594306823
2215	8	-0.18655349310660796
2215	9	-0.17286886279695324
2215	10	-0.1162133050584444
2215	11	-0.45902580783299385
2215	12	-0.34159963483400446
2215	13	-0.21513898646735186
2215	14	-0.07112436600993988
2215	15	-0.49818275536917156
2215	16	-0.2563879549943877
2215	17	-0.18781995239774574
2215	18	-0.13375129405989128
2215	19	-2.0963296636179294
2215	20	-2.3262327518959935
2215	21	-3.0337013786279456
2215	22	-2.1087863591951646
2215	23	-3.2028073095682004
2215	24	-2.2719208915421802
2215	25	-1.5937351140939597
2216	1	-1.4715986896954156
2216	2	-1.0868748463030435
2216	3	-0.707900087913174
2216	4	-0.8104511995057138
2216	5	-1.6374314015632265
2216	6	-1.292714721646064
2216	7	-0.9449800806456707
2216	8	-4.949152183889595
2216	9	-3.8777120294245115
2216	10	-1.8628238098860948
2216	11	-0.9055566792221512
2216	12	-1.044573992547818
2216	13	-1.7238848889682359
2216	14	-3.189253570012994
2216	15	-1.6777809899110991
2216	16	-2.8483891257285237
2216	17	-2.444157204513104
2216	18	-2.5432805745628286
2216	19	-1.5663676292913067
2216	20	-0.8309835351035559
2216	21	-0.6760486048752554
2216	22	-0.5857892328977599
2216	23	-0.7966492373804144
2216	24	-0.4439190488149052
2216	25	-0.137646603891298
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	6.0
7	147.0
8	678.0
9	1744.0
10	3122.0
11	5557.0
12	8730.0
13	12379.0
14	17238.0
15	23082.0
16	28258.0
17	30627.0
18	31360.0
19	31973.0
20	33001.0
21	34778.0
22	35702.0
23	37512.0
24	39486.0
25	42019.0
26	43923.0
27	48123.0
28	54478.0
29	63575.0
30	76334.0
31	93117.0
32	123182.0
33	173878.0
34	252056.0
35	400941.0
36	703077.0
37	2319719.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25327962039389	19.801497846931547	11.942257960942564	24.002964571732
2	12.93699020779602	22.2003312994066	37.92168333647858	26.940995156318802
3	20.466704999209444	28.241186346065696	27.521759170138193	23.77034948458667
4	12.584929020309149	19.1175015014612	34.53098630063447	33.76658317759518
5	11.813695042864374	39.79108204603207	35.21509356097033	13.180129350133221
6	32.12011588650704	34.09294570870621	18.2908711070818	15.496067297704954
7	24.67386634972463	33.33208239346867	23.22003661829443	18.774014638512273
8	26.262382724461446	36.392536296451595	21.288767755123434	16.05631322396352
9	26.02099305458882	17.739416256723597	21.03609651454746	35.203494174140125
10	15.679059582225232	31.955398502451366	32.61544783371193	19.750094081611476
11	29.43752876195947	27.54508355250951	20.253157656944644	22.764230028586375
12	22.718317592772934	30.56428478132023	29.487160403183655	17.230237222723183
13	28.09276513309069	22.720748561748376	23.78539021485718	25.401096090303753
14	20.784159075108832	26.205655113074588	26.474766508755554	26.535419303061026
15	29.343890240591396	26.425785242483553	22.021760255613295	22.208564261311757
16	19.371430715922987	29.741415006303345	30.262643958547404	20.624510319226264
17	24.08076407401312	30.061419021551693	27.805907924930214	18.05190897950498
18	24.536373314928177	27.161770668795672	28.723930630118005	19.577925386158142
19	22.208655325822154	30.928344703529305	29.426451534452326	17.43654843619622
20	23.06648558237204	28.131576552910342	27.877330345638146	20.924607519079473
21	19.779593527340772	30.0815744410576	29.696793696629193	20.44203833497243
22	21.230694857151487	29.29734168870141	24.3573694942339	25.1145939599132
23	18.446881435700956	30.4151686094637	27.749037693864427	23.38891226097092
24	20.777667429599575	34.01645075392825	25.458142364245013	19.74773945222717
25	21.81710443370456	27.257449043916786	23.589724732086573	27.335721790292077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10238.0
1	10238.0
2	12129.5
3	14021.0
4	14021.0
5	14021.0
6	17657.0
7	21293.0
8	21293.0
9	21293.0
10	23270.5
11	25248.0
12	25248.0
13	25248.0
14	32912.5
15	40577.0
16	40577.0
17	40577.0
18	57848.0
19	75119.0
20	75119.0
21	75119.0
22	104316.5
23	133514.0
24	133514.0
25	133514.0
26	170738.0
27	207962.0
28	207962.0
29	207962.0
30	248203.0
31	288444.0
32	288444.0
33	288444.0
34	322376.5
35	356309.0
36	356309.0
37	356309.0
38	384485.5
39	412662.0
40	412662.0
41	412662.0
42	461538.0
43	510414.0
44	510414.0
45	510414.0
46	779142.0
47	1047870.0
48	1047870.0
49	1047870.0
50	910014.0
51	772158.0
52	772158.0
53	772158.0
54	574252.0
55	376346.0
56	376346.0
57	376346.0
58	295509.5
59	214673.0
60	214673.0
61	214673.0
62	175184.0
63	135695.0
64	135695.0
65	135695.0
66	104267.0
67	72839.0
68	72839.0
69	72839.0
70	52982.5
71	33126.0
72	33126.0
73	33126.0
74	23424.0
75	13722.0
76	13722.0
77	13722.0
78	9363.0
79	5004.0
80	5004.0
81	5004.0
82	3399.0
83	1794.0
84	1794.0
85	1794.0
86	1170.0
87	546.0
88	546.0
89	546.0
90	357.0
91	168.0
92	168.0
93	168.0
94	106.0
95	44.0
96	44.0
97	44.0
98	30.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03128011785811699
2	0.014738554191860756
3	0.021153913484477243
4	0.023376227487801906
5	0.02182480073076393
6	0.006478254971955864
7	0.0011740526810017102
8	0.0011111570016623328
9	7.337829256260688E-4
10	1.6772181157167286E-4
11	0.0010063308694300372
12	0.0013837049454663013
13	0.0025787228529144703
14	6.499220198402324E-4
15	0.002054592191752993
16	0.0023900358148963388
17	0.0022642444562175836
18	0.0026206533058073887
19	4.8220020826855953E-4
20	3.564088495898049E-4
21	0.08327387944533558
22	0.08061129568663528
23	0.08193210495276221
24	0.0010901917752158736
25	0.22648734130109777
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4769803.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.88898737394892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.3080458790595	54.987654652960536
2	7.350012677034738	8.950696581806653
3	1.235830904119339	2.257454769517749
4	0.36339117209597	0.8850608195822406
5	0.18841501844950717	0.5736199839717196
6	0.10889157543960477	0.3978178657242901
7	0.0692983987504616	0.29536565285862143
8	0.0547085271723979	0.26649174561979816
9	0.04084855772511741	0.22385045840118278
>10	0.236056923441857	2.689773397946343
>50	0.022021005344854332	0.9327560054134109
>100	0.01646661320832225	1.9957830593057175
>500	0.0021673859985427706	0.9123379808215846
>1k	0.002447048647104866	3.3414574792070098
>5k	3.495783781578375E-4	1.6406417228764754
>10k+	0.0010487351344735125	19.64923782398658
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	148136	3.1057047848726667	No Hit
CCCATGTACTCTGCGTTGATACCAC	146588	3.073250614333548	No Hit
GAGTACATGGGAAGCAGTGGTATCA	65714	1.377708890702614	No Hit
CATGTACTCTGCGTTGATACCACTG	52308	1.0966490649613831	No Hit
GCGTTGATACCACTGCTTCCCATGT	46371	0.9721785155487553	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	38171	0.8002636586877906	No Hit
GCTTCCCATGTACTCTGCGTTGATA	37641	0.7891520886711674	No Hit
GTATCAACGCAGAGTACATGGGAAG	37243	0.7808079285454766	No Hit
ACTCTGCGTTGATACCACTGCTTCC	30998	0.6498800893873394	No Hit
TATCAACGCAGAGTACATGGGAAGC	30080	0.6306340115094901	No Hit
GGTATCAACGCAGAGTACATGGGAA	24646	0.5167089709994312	No Hit
CAGTGGTATCAACGCAGAGTACATG	19882	0.41683063220850003	No Hit
GTGGTATCAACGCAGAGTACATGGG	19151	0.40150505167613837	No Hit
GTACATGGTAAGCAGTGGTATCAAC	17693	0.37093775151720104	No Hit
GCAGTGGTATCAACGCAGAGTACAT	16521	0.3463665061219509	No Hit
ATACCACTGCTTCCCATGTACTCTG	16042	0.33632416265409704	No Hit
GTACTCTGCGTTGATACCACTGCTT	15267	0.32007611215809123	No Hit
ACATGGGAAGCAGTGGTATCAACGC	14187	0.2974336675959154	No Hit
CATGGGAAGCAGTGGTATCAACGCA	14087	0.29533714495126945	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	13819	0.28971846426361847	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	13729	0.2878315938834371	No Hit
ACCATGTACTCTGCGTTGATACCAC	12876	0.26994825572460746	No Hit
TACCACTGCTTCCCATGTACTCTGC	12409	0.2601574949741111	No Hit
CTTCCCATGTACTCTGCGTTGATAC	12272	0.2572852589509462	No Hit
GATACCACTGCTTCCCATGTACTCT	12025	0.25210684801867084	No Hit
CCATGTACTCTGCGTTGATACCACT	11353	0.23801821584665028	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	11111	0.23294463104660715	No Hit
GTTGATACCACTGCTTCCCATGTAC	11010	0.23082714317551478	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	10912	0.22877255098376179	No Hit
CCACTGCTTCCCATGTACTCTGCGT	10887	0.22824842032260034	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9922	0.20801697680176726	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	9254	0.1940122055355326	No Hit
TTCCCATGTACTCTGCGTTGATACC	9080	0.19036425613384872	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	8195	0.1718100307287324	No Hit
TGATACCACTGCTTCCCATGTACTC	8137	0.17059404759483776	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7606	0.15946151235176798	No Hit
GCTTACCATGTACTCTGCGTTGATA	6475	0.13574984124082273	No Hit
CCCCATGTACTCTGCGTTGATACCA	6419	0.13457578855982102	No Hit
ATCAACGCAGAGTACATGGGAAGCA	6382	0.13380007518130205	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5608	0.1175729899117427	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.1930452892918216E-5	0.0
6	2.0965226446459108E-5	0.0	0.0	4.1930452892918216E-5	0.0
7	2.0965226446459108E-5	0.0	0.0	6.289567933937733E-5	0.0
8	2.0965226446459108E-5	0.0	0.0	6.289567933937733E-5	0.0
9	2.0965226446459108E-5	0.0	0.0	8.386090578583643E-5	0.0
10	4.1930452892918216E-5	0.0	0.0	1.6772181157167286E-4	0.0
11	4.1930452892918216E-5	0.0	0.0	1.6772181157167286E-4	0.0
12	6.289567933937733E-5	0.0	0.0	1.8868703801813198E-4	0.0
13	6.289567933937733E-5	0.0	0.0	2.306174909110502E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCAC	40	0.0052853613	14.248318	3
ACGACGT	40	0.0052886712	14.246973	5
ACACCGT	115	7.122435E-8	11.560942	6
TGCTACG	60	0.0058820136	11.082489	2
TAGAACG	60	0.0058856877	11.081559	4
TACACCG	120	1.2811688E-7	11.080978	5
CCGTGCG	60	0.0058962605	11.078888	9
GTACAAA	1025	0.0	10.93644	1
GTACTAG	230	0.0	10.738989	1
CGCAAAA	260	0.0	10.595347	2
GAACAAA	1880	0.0	10.257843	1
TAGACAG	520	0.0	10.228596	5
ATCGCTT	80	0.004497379	9.505357	16
GTCTACG	80	0.004518834	9.499874	1
TCCGTAC	90	0.0011156071	9.4988785	3
TGTTCGG	80	0.0045333174	9.496189	10
CTAGGAC	315	0.0	9.348102	3
GTGCTAC	275	0.0	9.327149	1
GTCCTGG	635	0.0	9.275468	1
ACTAGAC	275	0.0	8.980758	3
>>END_MODULE