FastQCFastQC Report
Wed 25 May 2016
SRR1294751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4769803
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1612853.3813765474171573No Hit
GTACATGGGAAGCAGTGGTATCAAC1427132.9920103618535188No Hit
GAGTACATGGGAAGCAGTGGTATCA700851.4693478955000867No Hit
CATGTACTCTGCGTTGATACCACTG602371.2628823454553575No Hit
GCGTTGATACCACTGCTTCCCATGT502711.053942898689946No Hit
ACGCAGAGTACATGGGAAGCAGTGG461430.9673984439189628No Hit
GCTTCCCATGTACTCTGCGTTGATA423370.8876047920637392No Hit
GTATCAACGCAGAGTACATGGGAAG397620.8336193339641071No Hit
ACTCTGCGTTGATACCACTGCTTCC358960.7525677685220962No Hit
TATCAACGCAGAGTACATGGGAAGC346630.7267176443136121No Hit
GGTATCAACGCAGAGTACATGGGAA272350.5709879422693138No Hit
CAGTGGTATCAACGCAGAGTACATG240220.5036266696968408No Hit
GTGGTATCAACGCAGAGTACATGGG204300.4283195763011596No Hit
GCAGTGGTATCAACGCAGAGTACAT187720.39355923085293043No Hit
CATGGGAAGCAGTGGTATCAACGCA183160.38399908759334506No Hit
ATACCACTGCTTCCCATGTACTCTG181080.37963832049248153No Hit
ACATGGGAAGCAGTGGTATCAACGC180210.3778143457916396No Hit
GTACATGGTAAGCAGTGGTATCAAC167660.35150298660133344No Hit
GGGAAGCAGTGGTATCAACGCAGAG155580.3261769930540108No Hit
GTACTCTGCGTTGATACCACTGCTT151710.3180634504192312No Hit
CTGCTTCCCATGTACTCTGCGTTGA149640.3137236485448141No Hit
ACCATGTACTCTGCGTTGATACCAC147990.3102643861811484No Hit
CTTCCCATGTACTCTGCGTTGATAC144800.3035764789447279No Hit
GATACCACTGCTTCCCATGTACTCT140060.2936389616091063No Hit
TACCACTGCTTCCCATGTACTCTGC136560.2863011323528456No Hit
CCATGTACTCTGCGTTGATACCACT136400.2859656887297023No Hit
AAGCAGTGGTATCAACGCAGAGTAC132750.2783133810767447No Hit
GCAGAGTACATGGGAAGCAGTGGTA127430.26715988060722845No Hit
CCACTGCTTCCCATGTACTCTGCGT121150.2539937183988521No Hit
GTTGATACCACTGCTTCCCATGTAC117430.24619465416076933No Hit
GTATCAACGCAGAGTACTTTTTTTT117110.24552376691448263No Hit
ATGGGAAGCAGTGGTATCAACGCAG114520.2400937732648497No Hit
GTACATGGGTAAGCAGTGGTATCAA101830.2134889009042931No Hit
TTCCCATGTACTCTGCGTTGATACC94890.1989390337504505No Hit
GGTAAGCAGTGGTATCAACGCAGAG94800.19875034671243236No Hit
TATCAACGCAGAGTACTTTTTTTTT89710.18807904645118467No Hit
TGATACCACTGCTTCCCATGTACTC87230.18287967029246283No Hit
GGTATCAACGCAGAGTACTTTTTTT82930.1738646229204854No Hit
ATCAACGCAGAGTACATGGGAAGCA79550.16677837638158222No Hit
CCCCATGTACTCTGCGTTGATACCA74780.15677796336662123No Hit
GCTTACCATGTACTCTGCGTTGATA72460.1519140308310427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA64440.1350999192209825No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61630.12920869058952747No Hit
GAGTACATGGTAAGCAGTGGTATCA61430.1287893860605983No Hit
GTACTTTTTTTTTTTTTTTTTTTTT59060.12382062739278751No Hit
GGAAGCAGTGGTATCAACGCAGAGT52780.11065446518441117No Hit
AAAAAGTACTCTGCGTTGATACCAC51760.10851601208687237No Hit
ACTCTGCGTTGATACCACTGCTTAC50290.10543412379924286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGT604.1126303E-412.6601396
CCGAGAC550.0030343312.1098153
TGTACCG657.924211E-411.710595
ACAGCGT907.5212574E-611.603918
TTCGCCA600.005906346311.07634410
ATAACGC700.00147305610.874123
GCACCGT1355.7405487E-810.5501166
CACGTAC2001.8189894E-1210.466343
CTACGCA856.504172E-410.0745524
TAGGCCG856.504172E-410.0745525
CGCAGAA1909.640644E-1110.0157422
ATTAACG1054.0391074E-59.9679433
CTGTGCG1554.1103704E-89.8002679
ACGTACT2054.0017767E-119.7469244
GTACTAG2450.09.713731
CCACGTA2106.548362E-119.5149552
TACGCAG800.00446047879.5148545
TTAACGC1106.726694E-59.5148544
TGGACAG5250.09.4242375
AGTACTC14550.09.2859755
Produced by FastQC (version 0.11.5)