FastQCFastQC Report
Wed 25 May 2016
SRR1294750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671795
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC97230.26480236505578336No Hit
CCCATGTACTCTGCGTTGATACCAC88240.24031842736318337No Hit
GTATCAACGCAGAGTACTTTTTTTT52300.1424371458646248No Hit
GAGTACATGGGAAGCAGTGGTATCA42430.11555656021101396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG751.4822952E-512.66465955
GCGCGCC1157.1306204E-811.55976311
TATCGCG752.0838673E-411.3949347
ACCGCCC700.001497954210.852178
TCGCGCG803.7888414E-410.6827519
CTAGGAC4150.010.0711053
GACTCGC856.6261756E-410.0540811
TTAGACT3600.010.0261894
CGCGCCT1252.5881182E-69.875339512
ATCGCGC1356.490627E-79.847348
CCGTGCG800.00453451839.4957789
TGGACTG6550.09.2809725
GTCTAGC2800.09.1623831
CCGGAAC950.00182664368.9989413
GTATTAG5350.08.8801221
GTCTATA3000.08.8682821
ACACCGT1502.7199821E-68.8628476
GTGTAAG6500.08.7708281
TAGACTG5200.08.7678415
ACCGTCC1201.7737006E-48.7043458
Produced by FastQC (version 0.11.5)