##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294750_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3671795 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.943822844140264 33.0 33.0 33.0 27.0 33.0 2 30.97912437922052 33.0 33.0 33.0 27.0 33.0 3 30.809122241301598 33.0 33.0 33.0 27.0 33.0 4 34.15618464538461 37.0 33.0 37.0 27.0 37.0 5 34.478884033558515 37.0 37.0 37.0 27.0 37.0 6 34.71570335489863 37.0 37.0 37.0 27.0 37.0 7 34.66700428536996 37.0 37.0 37.0 27.0 37.0 8 34.59857127099961 37.0 37.0 37.0 27.0 37.0 9 34.281426931514424 37.0 37.0 37.0 27.0 37.0 10 34.353846824237195 37.0 37.0 37.0 27.0 37.0 11 34.62489191253869 37.0 37.0 37.0 27.0 37.0 12 34.53055576359791 37.0 37.0 37.0 27.0 37.0 13 34.46683052839279 37.0 37.0 37.0 27.0 37.0 14 35.575590685209825 37.0 37.0 40.0 27.0 40.0 15 35.90110940289423 40.0 37.0 40.0 27.0 40.0 16 35.93506282349641 40.0 37.0 40.0 27.0 40.0 17 35.87972503911575 40.0 37.0 40.0 27.0 40.0 18 35.88875985723604 40.0 37.0 40.0 27.0 40.0 19 35.9693980736942 40.0 37.0 40.0 27.0 40.0 20 35.84577189085992 40.0 37.0 40.0 27.0 40.0 21 35.78330652991248 40.0 37.0 40.0 27.0 40.0 22 35.655302651700325 37.0 37.0 40.0 27.0 40.0 23 35.275631945683244 37.0 33.0 40.0 27.0 40.0 24 35.581671907064525 37.0 37.0 40.0 27.0 40.0 25 34.42179179393185 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44592432458263787 1101 2 0.5908886632570578 1101 3 0.3602696626990003 1101 4 0.3747693138072634 1101 5 0.3728549466111488 1101 6 0.2335465102353993 1101 7 0.30038018081885554 1101 8 0.3845644068291989 1101 9 0.7248005996959677 1101 10 0.7542098081941262 1101 11 0.44194126031960224 1101 12 0.4568047197336327 1101 13 0.5222252361516908 1101 14 1.145154385799536 1101 15 0.8375142751860452 1101 16 0.8430697900349458 1101 17 0.8588921591423073 1101 18 0.8600660766422195 1101 19 0.9078516791500846 1101 20 0.9306149703250242 1101 21 0.9314681464948436 1101 22 1.0262861023613254 1101 23 1.3222369536106413 1101 24 1.0588302993535734 1101 25 0.9338941714496087 1102 1 0.25870494796689414 1102 2 0.44988045760703343 1102 3 0.20906422218709864 1102 4 0.11264116515886258 1102 5 0.19223098603998778 1102 6 0.19373315709374594 1102 7 0.2544249071040312 1102 8 0.331483895200563 1102 9 0.5228742999315585 1102 10 0.44983798534193653 1102 11 0.32765467353139144 1102 12 0.413946217419209 1102 13 0.40116921475443235 1102 14 0.9316036670136043 1102 15 0.8107630324046369 1102 16 0.4995474448494406 1102 17 0.6965640437654699 1102 18 0.721623028328942 1102 19 0.6354759736316637 1102 20 0.7209133931868976 1102 21 0.7278418273255411 1102 22 0.8542286202904705 1102 23 1.1167750056583543 1102 24 0.9139331020856076 1102 25 0.7225316517732452 1103 1 0.17185733814050508 1103 2 0.48762559454970855 1103 3 0.06716591317221443 1103 4 -0.12731720029350413 1103 5 0.12406499027866147 1103 6 0.12476100524393274 1103 7 0.18637398776932912 1103 8 0.30443520700281823 1103 9 0.48739401198860577 1103 10 0.5013266981905389 1103 11 0.41570615185803206 1103 12 0.31535739601574875 1103 13 0.3637154092270407 1103 14 0.8726609692080984 1103 15 0.6578772882863007 1103 16 0.5123187198410406 1103 17 0.4472028128238179 1103 18 0.5494609993400346 1103 19 0.7042022035346918 1103 20 0.6897523531306433 1103 21 0.7234134379883486 1103 22 0.6296945676748393 1103 23 1.1456370015908277 1103 24 0.8184760502642945 1103 25 0.7027338136228067 1104 1 0.30682537552852196 1104 2 0.5465017746736329 1104 3 0.3201694996864859 1104 4 0.32138781333470945 1104 5 0.1972001186399126 1104 6 0.15552752221142896 1104 7 0.13833962370475206 1104 8 0.411822704465834 1104 9 0.6045186300786369 1104 10 0.5461351745145038 1104 11 0.3764139333544563 1104 12 0.382121932431879 1104 13 0.36144628852918714 1104 14 0.91695101509859 1104 15 0.7370880372044226 1104 16 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2214 25 -1.999906418416927 2215 1 -0.23197759328233047 2215 2 -0.4408921610716021 2215 3 -0.8994582559350413 2215 4 -0.3763384674015384 2215 5 -0.21457003443739353 2215 6 -0.14698317314430653 2215 7 -0.22136493864564954 2215 8 -0.2804748864897988 2215 9 -0.19233999961383574 2215 10 -0.3114772222999562 2215 11 -0.6040122222538216 2215 12 -0.6097029817372004 2215 13 -0.4371652268103645 2215 14 -0.3081072436648 2215 15 -0.800740401050895 2215 16 -0.46807462830400226 2215 17 -0.40876934301694945 2215 18 -0.38696296396624064 2215 19 -2.3043862410365037 2215 20 -2.538455613409475 2215 21 -3.2496361204096473 2215 22 -2.4389060702719334 2215 23 -3.4164232921740556 2215 24 -2.499943378894791 2215 25 -1.8683785207197943 2216 1 -1.4589333849167474 2216 2 -1.181581383304625 2216 3 -0.7890531512319079 2216 4 -0.8048770991094116 2216 5 -1.7136230887664183 2216 6 -1.4247995361066828 2216 7 -1.1348364338774388 2216 8 -5.119854692315261 2216 9 -4.012001116001045 2216 10 -1.9760801297390316 2216 11 -1.037804435141588 2216 12 -1.245459572950793 2216 13 -2.0655606078321966 2216 14 -3.361327816080639 2216 15 -2.0337316440351856 2216 16 -2.984577117538386 2216 17 -2.6930899518844527 2216 18 -2.64943462144106 2216 19 -1.6096054861900129 2216 20 -0.9472545604702205 2216 21 -0.6663332932222019 2216 22 -0.7810874611980836 2216 23 -1.0493001032155362 2216 24 -0.5376717423990769 2216 25 -0.34083112866704823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 13.0 7 170.0 8 756.0 9 1656.0 10 2940.0 11 5010.0 12 7779.0 13 11236.0 14 14369.0 15 18018.0 16 20268.0 17 21623.0 18 22363.0 19 22859.0 20 23774.0 21 25247.0 22 26555.0 23 28168.0 24 29858.0 25 32224.0 26 34894.0 27 39420.0 28 44558.0 29 52660.0 30 62706.0 31 76838.0 32 101274.0 33 142296.0 34 205145.0 35 318925.0 36 549327.0 37 1728865.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.980331666790164 20.185295771455472 13.28143729540531 23.552935266349053 2 15.536961544391279 19.99284156598643 40.474570398622355 23.99562649099993 3 19.071777819002314 25.183732943461823 28.394098208285424 27.350391029250442 4 12.80043957864088 16.798327770495057 36.80340569926675 33.59782695159731 5 13.788610258183137 37.9941204498665 34.2486110918141 13.96865820013627 6 32.933046930668475 37.28045852027953 16.52455487011643 13.261939678935567 7 27.73616129329217 32.30038386304472 21.889835064720714 18.073619778942383 8 24.992258255341667 37.53105209016211 19.838503257252253 17.638186397243967 9 24.4725978577901 17.589168186655023 21.290250185952882 36.64798376960199 10 15.263938112810017 30.46068687830449 32.47258159883412 21.802793410051386 11 33.58728313655469 25.447235534924612 21.93487907448356 19.030602254037138 12 21.579056742883274 27.09717324641021 30.587017039179408 20.736752971527103 13 27.254920331268533 23.35850057629918 26.04806923433915 23.338509858093136 14 20.873479948253557 22.76669163205556 27.42223735276095 28.93759106692994 15 23.526572877104442 30.422080265381517 23.164941532452996 22.88640532506104 16 22.44994505292486 30.651534773259907 25.166223413309584 21.732296760505648 17 21.030290023378793 29.860604231713666 27.65857487422733 21.450530870680208 18 21.363576198260315 29.441991144151835 28.337243422525493 20.85718923506236 19 21.84194636986594 28.395772185635355 28.632660081121443 21.129621363377264 20 22.763863848051898 28.04310171088682 27.993316593042067 21.19971784801922 21 22.903644342682693 28.176956415078912 27.601957096519204 21.31744214571919 22 21.958023621967836 27.970360380422 28.340337754945455 21.731278242664708 23 21.984481189957148 28.199446255190335 28.189606627493504 21.626465927359018 24 21.97170885046881 27.869443145487345 28.14702203227944 22.01182597176441 25 22.390882827528955 27.73284640568284 27.448344239431695 22.42792652735651 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2853.0 1 2853.0 2 3275.5 3 3698.0 4 3698.0 5 3698.0 6 5055.0 7 6412.0 8 6412.0 9 6412.0 10 9456.0 11 12500.0 12 12500.0 13 12500.0 14 20207.5 15 27915.0 16 27915.0 17 27915.0 18 44266.5 19 60618.0 20 60618.0 21 60618.0 22 88088.5 23 115559.0 24 115559.0 25 115559.0 26 152648.5 27 189738.0 28 189738.0 29 189738.0 30 230855.5 31 271973.0 32 271973.0 33 271973.0 34 309277.5 35 346582.0 36 346582.0 37 346582.0 38 376528.0 39 406474.0 40 406474.0 41 406474.0 42 414252.5 43 422031.0 44 422031.0 45 422031.0 46 434487.5 47 446944.0 48 446944.0 49 446944.0 50 426997.5 51 407051.0 52 407051.0 53 407051.0 54 371426.5 55 335802.0 56 335802.0 57 335802.0 58 296712.5 59 257623.0 60 257623.0 61 257623.0 62 217321.5 63 177020.0 64 177020.0 65 177020.0 66 138725.5 67 100431.0 68 100431.0 69 100431.0 70 74630.0 71 48829.0 72 48829.0 73 48829.0 74 34798.0 75 20767.0 76 20767.0 77 20767.0 78 14084.0 79 7401.0 80 7401.0 81 7401.0 82 4938.5 83 2476.0 84 2476.0 85 2476.0 86 1613.0 87 750.0 88 750.0 89 750.0 90 510.0 91 270.0 92 270.0 93 270.0 94 166.0 95 62.0 96 62.0 97 62.0 98 39.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030584496138809494 2 0.01636801618826759 3 0.022931563445126976 4 0.027425278372022403 5 0.030121507328159658 6 0.013807960411733227 7 0.0047115920142600555 8 0.0038673183007221263 9 0.004520949562816007 10 0.002233240145487425 11 0.001960893786281642 12 0.005392457912274514 13 0.0024783518687726304 14 0.001225558616426026 15 0.0027234635920578357 16 0.002178770873646268 17 0.0024783518687726304 18 0.0026417596842961003 19 4.3575417472925364E-4 20 4.0851953880867534E-4 21 0.08429119817419001 22 0.07957960615992996 23 0.0805328184171502 24 0.0012527932523466042 25 0.23296507566462726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3671795.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.10713320528266 #Duplication Level Percentage of deduplicated Percentage of total 1 83.52552291546279 60.22786006906165 2 11.185981301956332 16.131780875439325 3 2.7827275748885176 6.019635237494985 4 1.0293393331980272 2.9689083364938798 5 0.47267119469603086 1.7041482399123393 6 0.2606710378179939 1.1277744748020817 7 0.16190241081668577 0.817202309211061 8 0.100771522022536 0.5813076449422463 9 0.07206995592434594 0.4677082120742097 >10 0.3693869399213273 4.777308567469891 >50 0.02630732477929123 1.3206164601816042 >100 0.010523175661171854 1.2833899851356212 >500 0.0012524165229800465 0.6622598700297043 >1k 7.590402885807044E-4 1.259415729829461 >5k 1.1385604328710536E-4 0.6506839879219284 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 9723 0.26480236505578336 No Hit CCCATGTACTCTGCGTTGATACCAC 8824 0.24031842736318337 No Hit GTATCAACGCAGAGTACTTTTTTTT 5230 0.1424371458646248 No Hit GAGTACATGGGAAGCAGTGGTATCA 4243 0.11555656021101396 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.7234635920578356E-5 0.0 5 0.0 0.0 0.0 2.7234635920578356E-5 0.0 6 2.7234635920578356E-5 0.0 0.0 2.7234635920578356E-5 0.0 7 2.7234635920578356E-5 0.0 0.0 2.7234635920578356E-5 0.0 8 2.7234635920578356E-5 0.0 0.0 2.7234635920578356E-5 0.0 9 2.7234635920578356E-5 0.0 0.0 2.7234635920578356E-5 0.0 10 2.7234635920578356E-5 0.0 0.0 2.7234635920578356E-5 0.0 11 2.7234635920578356E-5 0.0 0.0 8.170390776173507E-5 0.0 12 2.7234635920578356E-5 0.0 0.0 8.170390776173507E-5 0.0 13 2.7234635920578356E-5 0.0 0.0 1.0893854368231342E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTATCG 75 1.4822952E-5 12.6646595 5 GCGCGCC 115 7.1306204E-8 11.559763 11 TATCGCG 75 2.0838673E-4 11.394934 7 ACCGCCC 70 0.0014979542 10.85217 8 TCGCGCG 80 3.7888414E-4 10.682751 9 CTAGGAC 415 0.0 10.071105 3 GACTCGC 85 6.6261756E-4 10.05408 11 TTAGACT 360 0.0 10.026189 4 CGCGCCT 125 2.5881182E-6 9.8753395 12 ATCGCGC 135 6.490627E-7 9.84734 8 CCGTGCG 80 0.0045345183 9.495778 9 TGGACTG 655 0.0 9.280972 5 GTCTAGC 280 0.0 9.162383 1 CCGGAAC 95 0.0018266436 8.998941 3 GTATTAG 535 0.0 8.880122 1 GTCTATA 300 0.0 8.868282 1 ACACCGT 150 2.7199821E-6 8.862847 6 GTGTAAG 650 0.0 8.770828 1 TAGACTG 520 0.0 8.767841 5 ACCGTCC 120 1.7737006E-4 8.704345 8 >>END_MODULE