FastQCFastQC Report
Wed 25 May 2016
SRR1294750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3671795
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT140680.3831368581306963No Hit
GGTATCAACGCAGAGTACTTTTTTT107720.29337149813647007No Hit
GTACATGGGAAGCAGTGGTATCAAC101270.275805157967697No Hit
TATCAACGCAGAGTACTTTTTTTTT95630.26044482330849084No Hit
CCCATGTACTCTGCGTTGATACCAC93770.2553791810272632No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53350.1452967826362855No Hit
GAGTACATGGGAAGCAGTGGTATCA48290.13151605686047288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA600.005907201711.07601716
CTGATCG951.6555234E-49.9938089
CTACACT5100.09.7020774
CTGTCGC2350.09.6961199
GTACTAG2700.09.51952651
CCGCTCG900.00112060499.4941179
AAGCGAC900.00112102379.4937314
GTGTAGG4650.09.4171661
GTATTAG4200.09.29287051
CTAAGAC4100.09.2834133
CTATACT5600.09.1756594
TGTCGCC2401.0913936E-119.09852910
GAACCGC1151.11724934E-49.0825666
ACCGCTC950.00183477618.9945498
CTGTTCG850.00747667448.9356399
GGACTGT6100.08.872766
GTATTAA5150.08.8725681
GCTACAC3650.08.8637523
GCACCGA1201.7741842E-48.7041266
TCTGTCG2401.03682396E-108.7030598
Produced by FastQC (version 0.11.5)