FastQCFastQC Report
Wed 25 May 2016
SRR1294749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4541597
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1320102.906686788810192No Hit
CCCATGTACTCTGCGTTGATACCAC1296242.854150203111373No Hit
GAGTACATGGGAAGCAGTGGTATCA595331.3108384561642084No Hit
CATGTACTCTGCGTTGATACCACTG473801.0432453606077334No Hit
GCGTTGATACCACTGCTTCCCATGT418150.9207113709120381No Hit
ACGCAGAGTACATGGGAAGCAGTGG338230.7447380293760102No Hit
GCTTCCCATGTACTCTGCGTTGATA333820.7350277886831439No Hit
GTATCAACGCAGAGTACATGGGAAG328060.7223450253291959No Hit
ACTCTGCGTTGATACCACTGCTTCC285230.6280389915705863No Hit
TATCAACGCAGAGTACATGGGAAGC266730.5873044217705797No Hit
GGTATCAACGCAGAGTACATGGGAA224340.493967210212619No Hit
CAGTGGTATCAACGCAGAGTACATG183580.4042190445343345No Hit
GTGGTATCAACGCAGAGTACATGGG176010.3875508989458994No Hit
GTACATGGTAAGCAGTGGTATCAAC149870.3299940527528092No Hit
GCAGTGGTATCAACGCAGAGTACAT146650.32290403573897025No Hit
ATACCACTGCTTCCCATGTACTCTG144840.3189186535044831No Hit
GTACTCTGCGTTGATACCACTGCTT135230.29775869589485815No Hit
ACATGGGAAGCAGTGGTATCAACGC127990.2818171669569096No Hit
CATGGGAAGCAGTGGTATCAACGCA127330.2803639336559364No Hit
CTGCTTCCCATGTACTCTGCGTTGA121610.26776924504750205No Hit
GGGAAGCAGTGGTATCAACGCAGAG120570.26547930166415035No Hit
TACCACTGCTTCCCATGTACTCTGC112190.24702764247906628No Hit
CTTCCCATGTACTCTGCGTTGATAC112170.2469836051063095No Hit
GATACCACTGCTTCCCATGTACTCT108750.23945321436490294No Hit
ACCATGTACTCTGCGTTGATACCAC104770.23068977718630693No Hit
GTTGATACCACTGCTTCCCATGTAC99070.2181391259506293No Hit
AAGCAGTGGTATCAACGCAGAGTAC97190.21399961291149347No Hit
GCAGAGTACATGGGAAGCAGTGGTA97160.21393355685235832No Hit
CCATGTACTCTGCGTTGATACCACT96650.21281060384706085No Hit
CCACTGCTTCCCATGTACTCTGCGT95540.21036652965906047No Hit
GTACATGGGTAAGCAGTGGTATCAA87330.19228918814240895No Hit
ATGGGAAGCAGTGGTATCAACGCAG83460.18376795651397515No Hit
TTCCCATGTACTCTGCGTTGATACC82730.18216059240835328No Hit
TGATACCACTGCTTCCCATGTACTC74260.16351076504586382No Hit
GGTAAGCAGTGGTATCAACGCAGAG68880.1516647117742944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA65910.14512516191991495No Hit
ATCAACGCAGAGTACATGGGAAGCA56870.12522026943385772No Hit
CCCCATGTACTCTGCGTTGATACCA55780.1228202326186141No Hit
GCTTACCATGTACTCTGCGTTGATA52330.11522378581807237No Hit
GAGTACATGGTAAGCAGTGGTATCA45750.10073549018109709No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAC500.001504942113.29457413
TCGCACT802.8760212E-511.8722464
CGCAAAA4350.011.3550642
GTCCTAG3750.010.8939271
GTACAAA8000.010.4505691
AAGCGGT2400.010.28735410
TATTCCG1409.735413E-810.1763245
TCAGGAC4200.09.9508413
TGGACCG1151.0302323E-59.9108545
TAGACAG5350.09.5866675
ACGCAAA5550.09.5861081
CAGAACG1106.859845E-59.4977984
GTCTCGC2151.07320375E-109.2795751
TGTCGCC2151.07320375E-109.27518210
CGGTGGT2600.09.13088913
GAACAAA17500.09.1204981
GTAGCAC2501.8189894E-129.1185883
TTTAGAC3150.09.0462193
TTAGACA4750.08.9979134
GTGCTAC2651.8189894E-128.9627521
Produced by FastQC (version 0.11.5)