FastQCFastQC Report
Wed 25 May 2016
SRR1294749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4541597
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1413113.1114825908155215No Hit
GTACATGGGAAGCAGTGGTATCAAC1262812.780541734548442No Hit
GAGTACATGGGAAGCAGTGGTATCA630011.3871992605244365No Hit
CATGTACTCTGCGTTGATACCACTG535541.179188730307863No Hit
GCGTTGATACCACTGCTTCCCATGT447200.9846756548412376No Hit
ACGCAGAGTACATGGGAAGCAGTGG419250.9231334264136603No Hit
GCTTCCCATGTACTCTGCGTTGATA371970.8190290772166706No Hit
GTATCAACGCAGAGTACATGGGAAG352480.7761146574652044No Hit
ACTCTGCGTTGATACCACTGCTTCC318580.7014713106424899No Hit
TATCAACGCAGAGTACATGGGAAGC312340.6877316503423795No Hit
GGTATCAACGCAGAGTACATGGGAA244880.5391935920338154No Hit
CAGTGGTATCAACGCAGAGTACATG220390.4852698290931582No Hit
GTGGTATCAACGCAGAGTACATGGG191780.42227436736460766No Hit
GCAGTGGTATCAACGCAGAGTACAT165430.3642551287575714No Hit
CATGGGAAGCAGTGGTATCAACGCA164510.3622294096107603No Hit
ACATGGGAAGCAGTGGTATCAACGC164000.3611064566054628No Hit
ATACCACTGCTTCCCATGTACTCTG159280.35071363663486654No Hit
GGGAAGCAGTGGTATCAACGCAGAG141960.31257727182750916No Hit
GTACATGGTAAGCAGTGGTATCAAC136350.30022478876923686No Hit
CTGCTTCCCATGTACTCTGCGTTGA135380.29808897619053387No Hit
GTACTCTGCGTTGATACCACTGCTT131080.28862094104782965No Hit
CTTCCCATGTACTCTGCGTTGATAC127910.2816410174658826No Hit
AAGCAGTGGTATCAACGCAGAGTAC122670.2701032258036105No Hit
GATACCACTGCTTCCCATGTACTCT121940.2684958616979886No Hit
TACCACTGCTTCCCATGTACTCTGC120150.26455451683625825No Hit
ACCATGTACTCTGCGTTGATACCAC119350.26279302192598775No Hit
GCAGAGTACATGGGAAGCAGTGGTA114740.25264240750555367No Hit
CCATGTACTCTGCGTTGATACCACT113200.24925152980328288No Hit
GTATCAACGCAGAGTACTTTTTTTT111440.24537624100068764No Hit
GTTGATACCACTGCTTCCCATGTAC107130.23588618717160503No Hit
CCACTGCTTCCCATGTACTCTGCGT106070.23355220641549657No Hit
ATGGGAAGCAGTGGTATCAACGCAG102180.224986937414306No Hit
GTACATGGGTAAGCAGTGGTATCAA89970.19810212134630176No Hit
TTCCCATGTACTCTGCGTTGATACC87820.19336810377494965No Hit
TATCAACGCAGAGTACTTTTTTTTT86870.19127632856900337No Hit
GGTATCAACGCAGAGTACTTTTTTT82560.1817862747399208No Hit
TGATACCACTGCTTCCCATGTACTC78770.17344119260251403No Hit
GGTAAGCAGTGGTATCAACGCAGAG78430.17269255726564905No Hit
ATCAACGCAGAGTACATGGGAAGCA69140.1522371976201323No Hit
CCCCATGTACTCTGCGTTGATACCA65110.14336366700964442No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59540.13109925869688568No Hit
GCTTACCATGTACTCTGCGTTGATA59130.13019649255537202No Hit
GTACTTTTTTTTTTTTTTTTTTTTT58420.12863316582250692No Hit
GAGTACATGGTAAGCAGTGGTATCA50840.11194300154769346No Hit
AAAAAGTACTCTGCGTTGATACCAC49590.10919066575039572No Hit
GGAAGCAGTGGTATCAACGCAGAGT47440.10445664817904364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG400.00529906914.2427359
CTACGCA1501.2732926E-1112.0493514
GTCTACG802.818927E-511.8955321
TTAACGC802.8304083E-511.8908074
TACGCAG1459.458745E-1111.8088025
ATCGGTC1052.7425813E-711.75577714
TCTACGC1406.675691E-1011.551073
GTACCGT600.00589806411.0784146
AACACCG1053.4155255E-610.8715955
CGTGCGG700.001498721810.85160610
AAGACCG909.395824E-510.5696075
TAGACTG4800.010.30536655
GACAGTC3000.010.1281667
ATCTACG1553.986679E-89.8196132
GTTCTAG4150.09.631081
TAGACAG4850.09.6107145
TTCAACG800.00446855239.5127522
TTACGCT800.00446895939.5126464
CCATGCG900.00111959199.4951559
CTAGACT4250.09.4007334
Produced by FastQC (version 0.11.5)