##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294749_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4541597 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20182636196034 33.0 33.0 33.0 33.0 33.0 2 32.261068518408834 33.0 33.0 33.0 33.0 33.0 3 32.348171799479346 33.0 33.0 33.0 33.0 33.0 4 36.12279116795259 37.0 37.0 37.0 33.0 37.0 5 35.89824086108917 37.0 37.0 37.0 33.0 37.0 6 35.88004417829235 37.0 37.0 37.0 33.0 37.0 7 35.830243854749774 37.0 37.0 37.0 33.0 37.0 8 35.870929102692294 37.0 37.0 37.0 33.0 37.0 9 35.833212854421035 37.0 37.0 37.0 33.0 37.0 10 35.82734927823847 37.0 37.0 37.0 33.0 37.0 11 35.853421384592245 37.0 37.0 37.0 33.0 37.0 12 35.81772667191739 37.0 37.0 37.0 33.0 37.0 13 35.72096115089032 37.0 37.0 37.0 33.0 37.0 14 37.56440102457351 40.0 37.0 40.0 33.0 40.0 15 37.50338944648766 40.0 37.0 40.0 33.0 40.0 16 37.45215284403262 40.0 37.0 40.0 33.0 40.0 17 37.519363122707716 40.0 37.0 40.0 33.0 40.0 18 37.355178585858674 40.0 37.0 40.0 33.0 40.0 19 37.39073898454662 40.0 37.0 40.0 33.0 40.0 20 37.37829005964202 40.0 37.0 40.0 33.0 40.0 21 37.4160857953711 40.0 37.0 40.0 33.0 40.0 22 37.385062567198275 40.0 37.0 40.0 33.0 40.0 23 37.43884254811689 40.0 37.0 40.0 33.0 40.0 24 37.45590152538853 40.0 37.0 40.0 33.0 40.0 25 36.496124821290834 40.0 37.0 40.0 33.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2345756810371853 1101 2 0.1905349593372705 1101 3 0.23094189174724988 1101 4 0.24886477872197332 1101 5 0.4573443401167978 1101 6 0.3412326974938438 1101 7 0.4064992418776967 1101 8 0.3326742728897756 1101 9 0.30736521094021185 1101 10 0.37888147650507875 1101 11 0.36442092694230865 1101 12 0.3475498412525653 1101 13 0.41073472432629643 1101 14 0.6973587802719763 1101 15 0.6933575676204171 1101 16 0.6971191846941593 1101 17 0.6652550582489454 1101 18 0.7282730842920913 1101 19 0.7094560149363645 1101 20 0.7560900879304455 1101 21 0.7360471875446706 1101 22 0.7441560567122636 1101 23 0.7500300836727547 1101 24 0.7414563090156534 1101 25 0.8332951396196577 1102 1 0.26162841149233884 1102 2 0.24978016705285455 1102 3 0.22188107868072393 1102 4 0.31775964230455145 1102 5 0.4626605606129175 1102 6 0.30957652808412917 1102 7 0.45085472331456344 1102 8 0.35556413206470694 1102 9 0.39862046609290047 1102 10 0.3996840369719834 1102 11 0.41377332433565783 1102 12 0.39677864889696934 1102 13 0.4243811079499409 1102 14 0.6892450620680961 1102 15 0.6862916401439136 1102 16 0.6764209030779327 1102 17 0.7250697280682985 1102 18 0.7589046825846424 1102 19 0.7582449761568455 1102 20 0.776394171294335 1102 21 0.7311641593557141 1102 22 0.7897777979545566 1102 23 0.5680134670403163 1102 24 0.6586440112493221 1102 25 0.7477087266187823 1103 1 0.19660149807415905 1103 2 0.17945569790450833 1103 3 0.15330273686952012 1103 4 0.17722877728142805 1103 5 0.3799883184263564 1103 6 0.28312569302529766 1103 7 0.2926784152880728 1103 8 0.2477976865744722 1103 9 0.2445447825410625 1103 10 0.30708662577876567 1103 11 0.3497352060425456 1103 12 0.3092567891771054 1103 13 0.3128037150430103 1103 14 0.5150646849002953 1103 15 0.5958165320713391 1103 16 0.6152945886874193 1103 17 0.6016262910265837 1103 18 0.6634361122941144 1103 19 0.5730454244423129 1103 20 0.573635880924968 1103 21 0.5072008412125086 1103 22 0.5673240265084516 1103 23 0.5194153484127284 1103 24 0.5688638402436723 1103 25 0.6740768121440439 1104 1 0.19222762102878477 1104 2 0.17233505985837638 1104 3 0.16366069279152384 1104 4 0.13605062210640995 1104 5 0.3694346983478738 1104 6 0.2548197457845447 1104 7 0.2961051187286685 1104 8 0.2847348429430667 1104 9 0.30470417073721023 1104 10 0.24635910323220145 1104 11 0.3020924027688565 1104 12 0.2582899519613946 1104 13 0.2620981655566368 1104 14 0.3794041583909973 1104 15 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-1.9949557166724858 2215 16 -1.107456562316024 2215 17 -0.8352252214382574 2215 18 -1.6278749304342384 2215 19 -1.0103174351927109 2215 20 -1.2946717711051505 2215 21 -0.8967665173931678 2215 22 -0.6835285398027793 2215 23 -0.6961587165195766 2215 24 -0.6781019015923277 2215 25 -0.8540767952588624 2216 1 -1.178127472122128 2216 2 -0.611919950549332 2216 3 -0.3470877463525852 2216 4 -0.2971334958827754 2216 5 -4.709273014365564 2216 6 -2.1924563593856945 2216 7 -2.082229193637481 2216 8 -1.1084022454390237 2216 9 -0.8509373052766236 2216 10 -0.7974372834310799 2216 11 -0.632192499083807 2216 12 -0.777187434882741 2216 13 -0.8285420038011964 2216 14 -1.0953505971412199 2216 15 -1.911567817928109 2216 16 -2.355772620727066 2216 17 -1.9954769629464195 2216 18 -1.9286265895565933 2216 19 -1.4270000075844607 2216 20 -1.8030948041788903 2216 21 -1.881380394330641 2216 22 -1.6665691942961445 2216 23 -2.0893417252276407 2216 24 -2.512283615254894 2216 25 -2.1620743198150976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 10.0 8 74.0 9 118.0 10 155.0 11 335.0 12 718.0 13 1474.0 14 2821.0 15 4704.0 16 6348.0 17 7514.0 18 8459.0 19 9260.0 20 10176.0 21 11580.0 22 13301.0 23 15010.0 24 16992.0 25 19688.0 26 21372.0 27 24607.0 28 28560.0 29 34670.0 30 45538.0 31 62771.0 32 88332.0 33 131882.0 34 199883.0 35 338440.0 36 672102.0 37 2764703.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48669931553682 20.401285042611534 11.734825148206369 25.37719049364528 2 12.993391899280136 21.89289244391834 37.254904551246526 27.858811105555002 3 20.301316915613604 26.90758338971952 28.469478907970036 24.321620786696837 4 12.947692188452653 18.33687577299351 35.110556925240175 33.60487511331367 5 12.555323964869805 38.05849677687843 35.73044403480892 13.655735223442841 6 33.320531967227915 33.82487202671023 17.588350345791028 15.266245660270828 7 24.664011063959276 32.57471552382611 23.596588245578147 19.164685166636463 8 26.048205473286657 36.31072805405115 21.345791544423 16.295274928239188 9 25.491173119161516 16.339577564804774 21.732876071363695 36.436373244670015 10 15.707710214213963 30.582023607001513 33.25995137325846 20.450314805526062 11 30.52122944268171 25.852931915615084 20.545604273412057 23.08023436829114 12 22.406653348285502 29.45488510309048 30.289460107940098 17.849001440683917 13 28.605576408474814 21.43582973125973 24.995766907543757 24.9628269527217 14 20.932933179789455 24.148370944977422 27.42471249055947 27.493983384673648 15 29.190708362205385 25.528421880535646 22.593501965941876 22.687367791317094 16 19.91070541926111 28.824816468744363 30.26785511792438 20.996622994070147 17 23.4275960636754 29.518515183095285 28.76250358629354 18.291385166935772 18 23.98416239926176 26.555196332919895 29.594809931396377 19.86583133642197 19 22.29976281298689 29.96439112164884 29.839808488881804 17.896037576482463 20 23.092625787801076 26.727822834126407 28.737270171703916 21.442281206368598 21 19.86657997175883 29.59615307126546 29.71415121156721 20.8231157454085 22 21.32192706662436 28.675705924589963 25.37891406921398 24.623452939571695 23 18.34975670452486 29.11127957852711 28.87561357821929 23.663350138728735 24 20.817699148559416 32.347101691321356 26.598947462753742 20.236251697365486 25 22.01745209154199 26.40992152725623 24.62260182405631 26.95002455714547 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5313.0 1 5313.0 2 5521.0 3 5729.0 4 5729.0 5 5729.0 6 9062.5 7 12396.0 8 12396.0 9 12396.0 10 14015.5 11 15635.0 12 15635.0 13 15635.0 14 21794.5 15 27954.0 16 27954.0 17 27954.0 18 45046.0 19 62138.0 20 62138.0 21 62138.0 22 91958.0 23 121778.0 24 121778.0 25 121778.0 26 160195.5 27 198613.0 28 198613.0 29 198613.0 30 238381.0 31 278149.0 32 278149.0 33 278149.0 34 312836.0 35 347523.0 36 347523.0 37 347523.0 38 378277.5 39 409032.0 40 409032.0 41 409032.0 42 445806.0 43 482580.0 44 482580.0 45 482580.0 46 740681.5 47 998783.0 48 998783.0 49 998783.0 50 870878.0 51 742973.0 52 742973.0 53 742973.0 54 554698.0 55 366423.0 56 366423.0 57 366423.0 58 287275.5 59 208128.0 60 208128.0 61 208128.0 62 170674.5 63 133221.0 64 133221.0 65 133221.0 66 102436.5 67 71652.0 68 71652.0 69 71652.0 70 52439.5 71 33227.0 72 33227.0 73 33227.0 74 23274.5 75 13322.0 76 13322.0 77 13322.0 78 9035.5 79 4749.0 80 4749.0 81 4749.0 82 3193.5 83 1638.0 84 1638.0 85 1638.0 86 1055.0 87 472.0 88 472.0 89 472.0 90 301.5 91 131.0 92 131.0 93 131.0 94 83.0 95 35.0 96 35.0 97 35.0 98 19.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0436190177155745 2 0.0011009343189190939 3 0.0 4 0.0 5 0.21259041698327702 6 0.009137754847028478 7 8.807474551352751E-5 8 0.0026642610517842074 9 0.0015853454192434953 10 0.0013871772418380583 11 5.945045322163107E-4 12 8.807474551352751E-5 13 0.0 14 1.5413080464867315E-4 15 1.7614949102705502E-4 16 0.0 17 0.0 18 0.0 19 7.26616650486602E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.025695807003571648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4541597.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.838515748903404 #Duplication Level Percentage of deduplicated Percentage of total 1 87.68977455034096 50.71846406347685 2 9.062483368491197 10.483211740653067 3 1.692009319828967 2.935899230766572 4 0.5582247415274657 1.2914756201705542 5 0.26929957221357925 0.7787943774324027 6 0.1471918388202844 0.5108014492630242 7 0.10158080705251425 0.41126981759452147 8 0.0765126979800372 0.35403047096875767 9 0.05374323196706061 0.27975858916714236 >10 0.30365488027986975 3.2168848150864044 >50 0.023797809303613658 0.9661398639212183 >100 0.01561504091950636 1.7572924030342483 >500 0.0019100441697795793 0.7660938281070001 >1k 0.0025212582631544823 3.3208077974444894 >5k 4.5841059330081475E-4 1.9147374131827397 >10k+ 0.0012224282488021725 20.294338519731035 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 141311 3.1114825908155215 No Hit GTACATGGGAAGCAGTGGTATCAAC 126281 2.780541734548442 No Hit GAGTACATGGGAAGCAGTGGTATCA 63001 1.3871992605244365 No Hit CATGTACTCTGCGTTGATACCACTG 53554 1.179188730307863 No Hit GCGTTGATACCACTGCTTCCCATGT 44720 0.9846756548412376 No Hit ACGCAGAGTACATGGGAAGCAGTGG 41925 0.9231334264136603 No Hit GCTTCCCATGTACTCTGCGTTGATA 37197 0.8190290772166706 No Hit GTATCAACGCAGAGTACATGGGAAG 35248 0.7761146574652044 No Hit ACTCTGCGTTGATACCACTGCTTCC 31858 0.7014713106424899 No Hit TATCAACGCAGAGTACATGGGAAGC 31234 0.6877316503423795 No Hit GGTATCAACGCAGAGTACATGGGAA 24488 0.5391935920338154 No Hit CAGTGGTATCAACGCAGAGTACATG 22039 0.4852698290931582 No Hit GTGGTATCAACGCAGAGTACATGGG 19178 0.42227436736460766 No Hit GCAGTGGTATCAACGCAGAGTACAT 16543 0.3642551287575714 No Hit CATGGGAAGCAGTGGTATCAACGCA 16451 0.3622294096107603 No Hit ACATGGGAAGCAGTGGTATCAACGC 16400 0.3611064566054628 No Hit ATACCACTGCTTCCCATGTACTCTG 15928 0.35071363663486654 No Hit GGGAAGCAGTGGTATCAACGCAGAG 14196 0.31257727182750916 No Hit GTACATGGTAAGCAGTGGTATCAAC 13635 0.30022478876923686 No Hit CTGCTTCCCATGTACTCTGCGTTGA 13538 0.29808897619053387 No Hit GTACTCTGCGTTGATACCACTGCTT 13108 0.28862094104782965 No Hit CTTCCCATGTACTCTGCGTTGATAC 12791 0.2816410174658826 No Hit AAGCAGTGGTATCAACGCAGAGTAC 12267 0.2701032258036105 No Hit GATACCACTGCTTCCCATGTACTCT 12194 0.2684958616979886 No Hit TACCACTGCTTCCCATGTACTCTGC 12015 0.26455451683625825 No Hit ACCATGTACTCTGCGTTGATACCAC 11935 0.26279302192598775 No Hit GCAGAGTACATGGGAAGCAGTGGTA 11474 0.25264240750555367 No Hit CCATGTACTCTGCGTTGATACCACT 11320 0.24925152980328288 No Hit GTATCAACGCAGAGTACTTTTTTTT 11144 0.24537624100068764 No Hit GTTGATACCACTGCTTCCCATGTAC 10713 0.23588618717160503 No Hit CCACTGCTTCCCATGTACTCTGCGT 10607 0.23355220641549657 No Hit ATGGGAAGCAGTGGTATCAACGCAG 10218 0.224986937414306 No Hit GTACATGGGTAAGCAGTGGTATCAA 8997 0.19810212134630176 No Hit TTCCCATGTACTCTGCGTTGATACC 8782 0.19336810377494965 No Hit TATCAACGCAGAGTACTTTTTTTTT 8687 0.19127632856900337 No Hit GGTATCAACGCAGAGTACTTTTTTT 8256 0.1817862747399208 No Hit TGATACCACTGCTTCCCATGTACTC 7877 0.17344119260251403 No Hit GGTAAGCAGTGGTATCAACGCAGAG 7843 0.17269255726564905 No Hit ATCAACGCAGAGTACATGGGAAGCA 6914 0.1522371976201323 No Hit CCCCATGTACTCTGCGTTGATACCA 6511 0.14336366700964442 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5954 0.13109925869688568 No Hit GCTTACCATGTACTCTGCGTTGATA 5913 0.13019649255537202 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5842 0.12863316582250692 No Hit GAGTACATGGTAAGCAGTGGTATCA 5084 0.11194300154769346 No Hit AAAAAGTACTCTGCGTTGATACCAC 4959 0.10919066575039572 No Hit GGAAGCAGTGGTATCAACGCAGAGT 4744 0.10445664817904364 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.605605913514564E-5 0.0 5 0.0 0.0 0.0 6.605605913514564E-5 0.0 6 0.0 0.0 0.0 6.605605913514564E-5 0.0 7 0.0 0.0 0.0 6.605605913514564E-5 0.0 8 2.2018686378381877E-5 0.0 0.0 6.605605913514564E-5 0.0 9 2.2018686378381877E-5 0.0 0.0 8.807474551352751E-5 0.0 10 2.2018686378381877E-5 0.0 0.0 8.807474551352751E-5 0.0 11 4.4037372756763755E-5 0.0 0.0 8.807474551352751E-5 0.0 12 4.4037372756763755E-5 0.0 0.0 1.3211211827029128E-4 0.0 13 4.4037372756763755E-5 0.0 0.0 1.7614949102705502E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGCG 40 0.005299069 14.242735 9 CTACGCA 150 1.2732926E-11 12.049351 4 GTCTACG 80 2.818927E-5 11.895532 1 TTAACGC 80 2.8304083E-5 11.890807 4 TACGCAG 145 9.458745E-11 11.808802 5 ATCGGTC 105 2.7425813E-7 11.755777 14 TCTACGC 140 6.675691E-10 11.55107 3 GTACCGT 60 0.005898064 11.078414 6 AACACCG 105 3.4155255E-6 10.871595 5 CGTGCGG 70 0.0014987218 10.851606 10 AAGACCG 90 9.395824E-5 10.569607 5 TAGACTG 480 0.0 10.3053665 5 GACAGTC 300 0.0 10.128166 7 ATCTACG 155 3.986679E-8 9.819613 2 GTTCTAG 415 0.0 9.63108 1 TAGACAG 485 0.0 9.610714 5 TTCAACG 80 0.0044685523 9.512752 2 TTACGCT 80 0.0044689593 9.512646 4 CCATGCG 90 0.0011195919 9.495155 9 CTAGACT 425 0.0 9.400733 4 >>END_MODULE