FastQCFastQC Report
Wed 25 May 2016
SRR1294748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3484680
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51730.14844978592008448No Hit
GTACATGGGAAGCAGTGGTATCAAC49000.14061549410562807No Hit
CCCATGTACTCTGCGTTGATACCAC44430.12750094700230724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG802.0063126E-613.0596685
TATACCG802.8751272E-511.8724255
AGTACCG658.042428E-411.6897725
ACCGAGC855.3520896E-511.1713228
GTCTAGG3750.010.3900821
GTTCTAG4150.010.3045911
GCGTAAA750.002658811910.1288111
GTCTTAG3300.010.0790711
TGGACCG856.6104875E-410.0566435
GTCCAGG8250.09.9062871
CTAGGAC3950.09.8601793
GGACTGT4650.09.5985546
ACGGGTC800.00453502969.4956248
GACTGTC3500.09.4956247
GTATTAG4550.09.1898341
AGGACTG7150.09.1658455
GGACCGT1054.5118757E-49.04422956
TCTGTCG2850.08.9958548
TGTCGCC3200.08.90227410
TGCGTTG15600.08.88705712
Produced by FastQC (version 0.11.5)