##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294748_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3484680
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195157087594843	33.0	33.0	33.0	27.0	33.0
2	31.177588759943525	33.0	33.0	33.0	27.0	33.0
3	31.13886870530436	33.0	33.0	33.0	27.0	33.0
4	34.57989255828369	37.0	37.0	37.0	27.0	37.0
5	34.82031520828311	37.0	37.0	37.0	27.0	37.0
6	35.10184005418001	37.0	37.0	37.0	33.0	37.0
7	35.069513412996315	37.0	37.0	37.0	33.0	37.0
8	34.97739275916297	37.0	37.0	37.0	33.0	37.0
9	34.70361783578406	37.0	37.0	37.0	27.0	37.0
10	34.77266320006428	37.0	37.0	37.0	27.0	37.0
11	35.00166787194233	37.0	37.0	37.0	33.0	37.0
12	34.923307161633204	37.0	37.0	37.0	33.0	37.0
13	34.85989273046592	37.0	37.0	37.0	33.0	37.0
14	36.060934146033496	40.0	37.0	40.0	27.0	40.0
15	36.37534436447536	40.0	37.0	40.0	27.0	40.0
16	36.40411687730294	40.0	37.0	40.0	27.0	40.0
17	36.33690754961718	40.0	37.0	40.0	27.0	40.0
18	36.34343009975091	40.0	37.0	40.0	27.0	40.0
19	36.43595251213885	40.0	37.0	40.0	27.0	40.0
20	36.33048084759576	40.0	37.0	40.0	27.0	40.0
21	36.279749647026414	40.0	37.0	40.0	27.0	40.0
22	36.143763559351214	40.0	37.0	40.0	27.0	40.0
23	35.75606569326308	37.0	37.0	40.0	27.0	40.0
24	36.0655210808453	40.0	37.0	40.0	27.0	40.0
25	34.9611267031693	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.44014674049680735
1101	2	0.5416030473673388
1101	3	0.4961055640952843
1101	4	0.5000730291955762
1101	5	0.43281535192146237
1101	6	0.3845476397113785
1101	7	0.39973758489270494
1101	8	0.5137760375894942
1101	9	0.7399781757210135
1101	10	0.6777841163638527
1101	11	0.4687860234042631
1101	12	0.45328818118217384
1101	13	0.4334068221627092
1101	14	1.0488693245491731
1101	15	0.8818449092801686
1101	16	0.9223063977937542
1101	17	0.8916242780135093
1101	18	0.8333540243672033
1101	19	0.7650043638985906
1101	20	0.8938916096341174
1101	21	0.9207749739395723
1101	22	0.9905531736487205
1101	23	1.2361532277069784
1101	24	1.040661341517506
1101	25	0.9469168591001846
1102	1	0.32196697214011394
1102	2	0.46840534385747645
1102	3	0.3341986975322868
1102	4	0.3012295388970898
1102	5	0.2690965542819157
1102	6	0.29529588549798547
1102	7	0.3590315695869606
1102	8	0.42143492741563904
1102	9	0.5963316576919837
1102	10	0.7156109848672472
1102	11	0.4538602748485516
1102	12	0.5433601104440982
1102	13	0.5185316495711092
1102	14	1.0130376463883763
1102	15	0.8796440014281401
1102	16	0.612238906280723
1102	17	0.8002758221974062
1102	18	0.8217601808005455
1102	19	0.7950507985626203
1102	20	0.861018131292397
1102	21	0.8957124726482277
1102	22	0.9917497488564351
1102	23	1.331042001010772
1102	24	1.151524214178366
1102	25	0.9967157679888885
1103	1	0.28391117644865105
1103	2	0.45243582620939904
1103	3	0.20115562599077208
1103	4	0.12665195735225154
1103	5	0.24954028509942106
1103	6	0.26127036458604636
1103	7	0.33907424907705774
1103	8	0.4355583910974232
1103	9	0.6685038542419122
1103	10	0.5879981528945422
1103	11	0.39447959353542217
1103	12	0.43956659458466874
1103	13	0.44350808991198676
1103	14	1.0178190498619841
1103	15	0.7588805608565963
1103	16	0.4457750695101481
1103	17	0.5679688705631918
1103	18	0.6113535728901454
1103	19	0.6288675675097579
1103	20	0.7132177725904327
1103	21	0.8589504045055776
1103	22	0.7267391525150941
1103	23	1.15757565946938
1103	24	0.8313766049241451
1103	25	0.7512045307581872
1104	1	0.44145291923151575
1104	2	0.5911776923825016
1104	3	0.45199483894601045
1104	4	0.43960707044183067
1104	5	0.42682601082849203
1104	6	0.3280486112221439
1104	7	0.2956810219149162
1104	8	0.5387206407933434
1104	9	0.5957382960424766
1104	10	0.7309192967602058
1104	11	0.4528022718252629
1104	12	0.49659948254339525
1104	13	0.48640258157555394
1104	14	1.024015978716342
1104	15	0.8522760231581898
1104	16	0.8029172137971159
1104	17	0.8184001470296849
1104	18	0.678463418564597
1104	19	0.747499656135922
1104	20	0.8438160860939163
1104	21	0.7890623576886853
1104	22	0.9391465829999035
1104	23	1.418041036217673
1104	24	1.1484952913566033
1104	25	0.849433016090984
1105	1	0.3434509835798245
1105	2	0.33155780515528477
1105	3	0.3801934617014524
1105	4	0.30994939229170626
1105	5	0.3588999914226534
1105	6	0.3551664449021317
1105	7	0.3360749830893255
1105	8	0.452188142559919
1105	9	-1.5232381839556979
1105	10	-0.05600900240573736
1105	11	0.227961587029057
1105	12	0.28432837034266356
1105	13	0.33226856959242923
1105	14	-1.4732280852088877
1105	15	-0.1468063667969517
1105	16	-0.012572269917136225
1105	17	0.25455962928470655
1105	18	0.32482820941820734
1105	19	0.45321260350615233
1105	20	0.5619248221960902
1105	21	0.6696593781658393
1105	22	0.6196189864608712
1105	23	1.0386929570705803
1105	24	0.7272375894153456
1105	25	0.48250352736853586
1106	1	-0.02231156016964775
1106	2	0.05864977795233983
1106	3	0.07788010443583815
1106	4	-1.1034461660181876
1106	5	-0.354188470641013
1106	6	-0.09473205260415085
1106	7	-0.03826420130361896
1106	8	0.0941948225875393
1106	9	0.21909213488366674
1106	10	0.441175326040252
1106	11	0.16635325843678572
1106	12	0.003147633653341586
1106	13	0.1480851620259287
1106	14	0.5153750032648148
1106	15	0.324711341501299
1106	16	0.17004105820186766
1106	17	-0.04543469007489165
1106	18	0.20197153888397423
1106	19	0.12474637378699072
1106	20	0.2275643179429494
1106	21	0.38084567046891493
1106	22	0.4923187260187092
1106	23	0.727840692430533
1106	24	0.3810557212911263
1106	25	0.3647816601581795
1107	1	0.38979803121306134
1107	2	0.3078288374854772
1107	3	0.32680485628937106
1107	4	0.46946549794307657
1107	5	0.41974740801077104
1107	6	0.2149131567013427
1107	7	0.18804011688543198
1107	8	0.32359513024847786
1107	9	0.4213882452086324
1107	10	0.4204345811317509
1107	11	0.2632265778905918
1107	12	0.18852690241092063
1107	13	0.29495930089078115
1107	14	0.6982987628048676
1107	15	0.555376712650073
1107	16	0.565345756981678
1107	17	0.44924201832904487
1107	18	0.559154243836204
1107	19	0.5469166806258343
1107	20	0.636211931136998
1107	21	0.7298342649369403
1107	22	0.5886075620276827
1107	23	0.9092050703236154
1107	24	0.8516824698238779
1107	25	0.8036021762281749
1108	1	0.4648705983531052
1108	2	0.3789222316639318
1108	3	0.4833084071013616
1108	4	0.6585590690656318
1108	5	0.4828412819231076
1108	6	0.33463796850819705
1108	7	0.36833737030337943
1108	8	0.5231870608374081
1108	9	0.2173450514225408
1108	10	0.5279168460753496
1108	11	0.41561670626796854
1108	12	0.4109495205121334
1108	13	0.36201347091210323
1108	14	-0.9983347173413932
1108	15	0.015664945719656487
1108	16	0.3800942799313063
1108	17	0.5207922576786714
1108	18	0.6851742114921748
1108	19	0.5965047788336122
1108	20	0.6622795554555339
1108	21	0.7600361311270802
1108	22	0.8409569172480289
1108	23	1.1224305648855406
1108	24	0.9327607477397137
1108	25	0.826415549401375
1109	1	0.29017834990515823
1109	2	0.23205063841935925
1109	3	0.3464627275374248
1109	4	0.4326399418758413
1109	5	0.416085768351401
1109	6	0.22709355569347878
1109	7	0.22539750791396074
1109	8	0.28518254189999226
1109	9	0.40709804170810315
1109	10	0.33100912686220596
1109	11	0.17055873025833534
1109	12	0.18283424080181732
1109	13	0.22092645796377042
1109	14	0.5080102622553113
1109	15	0.35055803583033196
1109	16	0.3218321560307231
1109	17	0.3322662737885551
1109	18	0.3040756246175178
1109	19	0.2732700366300591
1109	20	0.5111097804237303
1109	21	0.4952155057095453
1109	22	0.5741872766780105
1109	23	0.9400843768347684
1109	24	0.6534371416106382
1109	25	0.5205478240608628
1110	1	0.41566206147438933
1110	2	0.2682228928428785
1110	3	0.33952087192060176
1110	4	0.5851259966032245
1110	5	0.5093186973997703
1110	6	0.268819321456526
1110	7	0.3103180225865927
1110	8	0.26187972079797817
1110	9	0.3941514930634469
1110	10	0.4244851805912475
1110	11	0.3060903536650841
1110	12	0.23572686241239893
1110	13	0.19694939792674404
1110	14	0.6799081029088114
1110	15	0.47100831146391897
1110	16	0.3622603507754434
1110	17	0.41005550029468196
1110	18	0.4585973493765536
1110	19	0.4761191760039196
1110	20	0.662871394073612
1110	21	0.6358626804547782
1110	22	0.7437864565307066
1110	23	0.8109470499814009
1110	24	0.7307621325291223
1110	25	0.6272542900196285
1111	1	0.061828053480816436
1111	2	0.036714051363016864
1111	3	0.2470339580800669
1111	4	0.4348542337785304
1111	5	0.2164979982793298
1111	6	0.16001245877901482
1111	7	0.10663122726590757
1111	8	0.1548850449013699
1111	9	0.22915696352386306
1111	10	0.07996440123521609
1111	11	0.14391181146251597
1111	12	0.052680644722791214
1111	13	0.08470823023891683
1111	14	-1.7178216030521085
1111	15	-0.48683904076967366
1111	16	-0.07240784660965716
1111	17	0.10238422468566455
1111	18	0.1931747173981293
1111	19	0.20611550403987877
1111	20	0.1706712291693364
1111	21	0.28105991284023446
1111	22	0.1271108829472709
1111	23	-2.012193820608182
1111	24	-0.37531795866205897
1111	25	-0.13950226072021366
1112	1	-0.1425088191496009
1112	2	-0.2879873661137111
1112	3	-0.08059695789493304
1112	4	0.1500940306337597
1112	5	-0.7269857959504975
1112	6	-1.0209100288993156
1112	7	-1.0142597644594957
1112	8	-0.7442644627083013
1112	9	-0.6029068315065516
1112	10	-0.9930658944785478
1112	11	-0.9903650873329894
1112	12	-0.8021291119698617
1112	13	-0.8457535091992625
1112	14	-0.6494764018166705
1112	15	-0.8546207522764036
1112	16	-0.8268595330123887
1112	17	-0.9010408728297961
1112	18	-1.116664384474788
1112	19	-0.8834670681571382
1112	20	-0.9858968556517951
1112	21	-1.8172184524200574
1112	22	-1.7133332055412964
1112	23	-1.4015120097910412
1112	24	-1.5967469612436247
1112	25	-1.9143839999931984
1113	1	-0.011520065967946636
1113	2	-0.17287635949486457
1113	3	-0.18930800876932352
1113	4	0.022941868869672533
1113	5	-0.1234331566791056
1113	6	-0.15444159020864845
1113	7	-0.21046180235205014
1113	8	-0.22987119432920622
1113	9	-0.004722377650821841
1113	10	-0.33907352856060413
1113	11	-0.22922101855080967
1113	12	-0.19030539992260742
1113	13	-0.3448301146729946
1113	14	-0.2160149956091999
1113	15	-0.32848291636013727
1113	16	-0.25385727183047635
1113	17	-0.19830953858159717
1113	18	-0.11705363213675923
1113	19	-0.3672888364806042
1113	20	-0.9253570574042911
1113	21	-0.7244622810027295
1113	22	-0.24180338427964898
1113	23	-0.4512179052712497
1113	24	-0.1870980573542269
1113	25	-1.3671443140818624
1114	1	-0.9094752005967983
1114	2	-0.7582094489356379
1114	3	-0.588122957769869
1114	4	-1.586978011182346
1114	5	-0.8831721419898528
1114	6	-0.6501572884341158
1114	7	-0.5659406734521895
1114	8	-0.5696717144161312
1114	9	-0.5160490306241314
1114	10	-0.5897564199783005
1114	11	-0.5793950092087314
1114	12	-0.661855968609629
1114	13	-0.6258080617895772
1114	14	-0.5954108216021012
1114	15	-1.4819049601829164
1114	16	-0.9590750056744994
1114	17	-0.9688472239387806
1114	18	-0.9687965563434204
1114	19	-0.9691153311254368
1114	20	-2.04989828106541
1114	21	-7.053095004092665
1114	22	-8.034436991092257
1114	23	-8.223392742726634
1114	24	-5.973828595325056
1114	25	-7.538267031293245
1115	1	-1.1983682485839928
1115	2	-1.0845792039727442
1115	3	-1.005153121630343
1115	4	-0.8849418606197901
1115	5	-0.7960430955671214
1115	6	-1.1735941113309067
1115	7	-1.1952594371442231
1115	8	-0.9798352389640073
1115	9	-2.8165435634094216
1115	10	-1.7183564434931995
1115	11	-1.3613033154879304
1115	12	-1.2298089004140138
1115	13	-1.4634444782144982
1115	14	-1.178329404853251
1115	15	-2.1504044454945017
1115	16	-1.522450401888598
1115	17	-3.193458112298181
1115	18	-2.892681314127934
1115	19	-2.2510369305226874
1115	20	-1.9005654084321932
1115	21	-1.4686234775196283
1115	22	-1.1304315394741664
1115	23	-0.5232988270875438
1115	24	-1.524222517922638
1115	25	-1.194770653307991
1116	1	-1.561618364130645
1116	2	-0.8618741782379047
1116	3	-0.7378786916806526
1116	4	-0.35119841532115004
1116	5	-0.3259305011031941
1116	6	-0.384305612517835
1116	7	-0.9244447062166827
1116	8	-1.8404047206132645
1116	9	-0.966529649414845
1116	10	-2.4792055117395293
1116	11	-1.2424317283488193
1116	12	-0.8449636845293114
1116	13	-0.8974755439296942
1116	14	-1.3621451303289263
1116	15	-1.3551957960715058
1116	16	-0.8256240993572206
1116	17	-0.8765465835252542
1116	18	-1.387129606094355
1116	19	-0.847143431866904
1116	20	-1.1977031908560392
1116	21	-0.6588009232973917
1116	22	-0.28243866343939317
1116	23	-0.10120669466503074
1116	24	-0.13226872690357538
1116	25	-0.16934090446164163
1201	1	0.31896406328975857
1201	2	0.32044151987065916
1201	3	0.3077654133966128
1201	4	0.4120981923818192
1201	5	0.5170170151862408
1201	6	0.4138902230149526
1201	7	0.4917060600226577
1201	8	0.5501234919260369
1201	9	0.7383970330015401
1201	10	0.7000040067347513
1201	11	0.5674801297237977
1201	12	0.5275146969094777
1201	13	0.5740101588012649
1201	14	1.232494852311703
1201	15	1.0106923378007053
1201	16	0.9972174451710458
1201	17	0.8335570649482662
1201	18	0.748498614548474
1201	19	0.8367294838148851
1201	20	0.9221329949121895
1201	21	0.9369274052750285
1201	22	0.99069910199173
1201	23	0.7600459173064849
1201	24	0.8830833824350535
1201	25	0.8365456902238648
1202	1	-0.11029733928020846
1202	2	0.16627326167424172
1202	3	0.0834549825941977
1202	4	0.09207126324265147
1202	5	0.2660715173694115
1202	6	0.1841405588695153
1202	7	0.08430118488215044
1202	8	0.3357666173227756
1202	9	0.28942231241946814
1202	10	0.532985000450978
1202	11	0.26537101474804103
1202	12	0.35036831962622017
1202	13	0.42728175141584046
1202	14	0.6649899850414798
1202	15	0.6746146602095422
1202	16	0.595813586786349
1202	17	0.49237684422170247
1202	18	0.4068675996675566
1202	19	0.44822278996990406
1202	20	0.5569112549348816
1202	21	0.6383200355882721
1202	22	0.6148527044402101
1202	23	0.880470478188613
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2211	6	0.17258643685027408
2211	7	0.12484014635134599
2211	8	0.20177343565517702
2211	9	0.40631816419904254
2211	10	0.38635772591673145
2211	11	0.16856394584794998
2211	12	0.19346626388695398
2211	13	0.09336837796757891
2211	14	0.41750319104306044
2211	15	0.30713876831504194
2211	16	0.2273278950448443
2211	17	0.2707689947068772
2211	18	0.34323906627157896
2211	19	0.29566230136290983
2211	20	0.2969487798561232
2211	21	0.34945493858364074
2211	22	0.4468506806242374
2211	23	0.6259801364889697
2211	24	0.5353633374453111
2211	25	0.6787904005780092
2212	1	-0.27000056036862574
2212	2	-0.285444617747622
2212	3	-0.19247465386674634
2212	4	-0.6160399171645494
2212	5	-0.23923545691289405
2212	6	-0.5670295128887943
2212	7	-0.5146783934143144
2212	8	-0.2806022236191197
2212	9	-0.25968428412545563
2212	10	-0.612357639199061
2212	11	-0.4290942922409826
2212	12	-0.6199578437739461
2212	13	-0.6651673343918816
2212	14	-0.43844135064792056
2212	15	-0.6670727584228544
2212	16	-0.4680946534027228
2212	17	-0.5099619723486128
2212	18	-0.42968962341718253
2212	19	-0.49439599752554386
2212	20	-0.5729400975919958
2212	21	-0.48940363226822825
2212	22	-0.5235085758565532
2212	23	-0.009864118692476609
2212	24	-0.30428039701771326
2212	25	-1.0436375920410654
2213	1	-0.1471032468349982
2213	2	-1.2530222220320972
2213	3	-0.29855073640911556
2213	4	0.06490079258196602
2213	5	0.0015375189810669099
2213	6	0.020272202713286447
2213	7	-0.02861629955715017
2213	8	0.05674893474961351
2213	9	0.13759339779426227
2213	10	-0.3790278693083664
2213	11	-0.11954105017089489
2213	12	0.013408560045370166
2213	13	-0.3467738429764111
2213	14	0.003057524586175475
2213	15	-0.16247709074342964
2213	16	-0.16362459707642785
2213	17	-0.01961755475311122
2213	18	0.06224133010140065
2213	19	0.12255716775670322
2213	20	0.08328463917497686
2213	21	-0.4750260537969808
2213	22	-0.8997111888595128
2213	23	-0.08704769965248005
2213	24	0.05532104930543369
2213	25	-0.4558120503706249
2214	1	-0.16795104555297868
2214	2	-0.37304980813047095
2214	3	-0.32724776475388495
2214	4	-0.5730962289709893
2214	5	-0.27072472968281147
2214	6	-0.2858752323823808
2214	7	-0.4955342710058446
2214	8	-0.13757870992958487
2214	9	-0.011950746616101071
2214	10	-1.8548104868729638
2214	11	-0.8494871819558938
2214	12	-0.46685075298186973
2214	13	-0.2956769073798
2214	14	-0.651147920688878
2214	15	-0.5478111027180788
2214	16	-0.7204727054177624
2214	17	-0.4643552826013604
2214	18	-0.4839145907104552
2214	19	-1.4103469083606512
2214	20	-2.335534918329749
2214	21	-1.7948803387539058
2214	22	-1.9515293061991272
2214	23	-2.4797297702736785
2214	24	-1.6146101613183959
2214	25	-1.9428308368484082
2215	1	-0.31578809559940524
2215	2	-0.3690931476997612
2215	3	-0.9381790041761384
2215	4	-0.39269514972535546
2215	5	-0.2001642853351555
2215	6	-0.30427087819570175
2215	7	-0.44277891485932486
2215	8	-0.34842393802759375
2215	9	-0.3117302074420607
2215	10	-0.4377724666820484
2215	11	-0.8041378333248375
2215	12	-0.5501503022420735
2215	13	-0.42687986957353274
2215	14	-0.25288677249699276
2215	15	-0.7614094372746933
2215	16	-0.6469905733460806
2215	17	-0.4691553144109051
2215	18	-0.435369057550119
2215	19	-2.391722224552254
2215	20	-2.5340683078258337
2215	21	-3.4613492227796456
2215	22	-2.3284491706876906
2215	23	-3.5345842538319445
2215	24	-2.4296017560890277
2215	25	-1.8123138841419646
2216	1	-1.5248231208272642
2216	2	-1.1145083138405028
2216	3	-0.7648205065951394
2216	4	-0.8814303543119877
2216	5	-1.776914959781699
2216	6	-1.5881501760127819
2216	7	-1.1698410802472097
2216	8	-5.157152498983152
2216	9	-4.099871879804613
2216	10	-1.9382250474943277
2216	11	-1.0220569392303105
2216	12	-1.1772018604552912
2216	13	-1.964715818119224
2216	14	-3.5908362334634916
2216	15	-2.1707911160473046
2216	16	-2.9715795092080697
2216	17	-2.6750981254855617
2216	18	-2.732626398563795
2216	19	-1.7658071149389656
2216	20	-1.0651469081940874
2216	21	-0.8220697954852056
2216	22	-0.8793407934156505
2216	23	-0.9812275164881044
2216	24	-0.658021423115251
2216	25	-0.43183416774483163
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	55.0
8	271.0
9	743.0
10	1591.0
11	2986.0
12	5198.0
13	7981.0
14	10337.0
15	13208.0
16	15008.0
17	15748.0
18	16844.0
19	17106.0
20	18044.0
21	19333.0
22	20915.0
23	22510.0
24	23707.0
25	26461.0
26	28898.0
27	32767.0
28	38079.0
29	44752.0
30	54279.0
31	67250.0
32	90324.0
33	129900.0
34	189854.0
35	302193.0
36	527567.0
37	1740771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97054321778659	19.881835290497335	13.424284908881932	23.72333658283414
2	15.812996218101624	19.595181602586973	40.516739858373256	24.075082320938147
3	19.463854309908605	24.106837639955533	28.443568453177953	27.98573959695791
4	12.582682042572587	16.390426004289925	37.26868043693719	33.7582115162003
5	13.85118427612645	37.39494556155851	34.866434731992605	13.887435430322439
6	32.95705210882222	37.14961304250247	16.647093499469918	13.246241349205393
7	28.130018446647636	31.747604633357096	21.5649001734467	18.55747674654857
8	24.807182156185036	37.26145284615093	19.777474605669	18.15389039199503
9	24.30608446309898	17.575415121076862	21.16131739063308	36.95718302519108
10	14.848256575596219	30.200435335964865	32.933782730908135	22.017525357530783
11	33.6970726944951	24.905743437338398	22.345793164167986	19.05139070399852
12	21.63825863562245	26.466590789472928	30.71393437024512	21.181216204659503
13	27.369529994025125	22.880341296575725	26.12947161132689	23.620657098072257
14	20.82918299896776	22.60912760760809	27.48644845880185	29.075240934622297
15	23.045794913104828	30.602700280462525	23.41722911748002	22.934275688952624
16	22.363150484676265	30.602446081236433	25.078732271248818	21.955671162838485
17	20.9478510591099	29.602158310750486	27.563316867441873	21.88667376269774
18	21.23147819454062	29.17653027929389	28.451312614987796	21.140678911177694
19	21.747931724896524	28.447338009629043	28.5896758571192	21.215054408355233
20	22.604561013630008	28.009701939699205	28.21078301953933	21.174954027131456
21	22.723121250485384	27.902649905910305	27.793510453261895	21.580718390342422
22	21.883775604157897	27.769407637206005	28.476740359005316	21.87007639963078
23	22.073789046636833	27.90783032149448	28.209990677573277	21.80838995429541
24	22.05853571184644	27.80629975762216	28.258885704758193	21.876278825773205
25	22.26379409469778	27.81801547723784	27.7760249780197	22.14216545004468
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1695.0
1	1695.0
2	2006.5
3	2318.0
4	2318.0
5	2318.0
6	3542.0
7	4766.0
8	4766.0
9	4766.0
10	7693.5
11	10621.0
12	10621.0
13	10621.0
14	17942.5
15	25264.0
16	25264.0
17	25264.0
18	41141.0
19	57018.0
20	57018.0
21	57018.0
22	84242.5
23	111467.0
24	111467.0
25	111467.0
26	147702.0
27	183937.0
28	183937.0
29	183937.0
30	223269.0
31	262601.0
32	262601.0
33	262601.0
34	296436.5
35	330272.0
36	330272.0
37	330272.0
38	355115.0
39	379958.0
40	379958.0
41	379958.0
42	388162.5
43	396367.0
44	396367.0
45	396367.0
46	402211.0
47	408055.0
48	408055.0
49	408055.0
50	394092.0
51	380129.0
52	380129.0
53	380129.0
54	352540.5
55	324952.0
56	324952.0
57	324952.0
58	287984.5
59	251017.0
60	251017.0
61	251017.0
62	211737.0
63	172457.0
64	172457.0
65	172457.0
66	136050.5
67	99644.0
68	99644.0
69	99644.0
70	74506.5
71	49369.0
72	49369.0
73	49369.0
74	35187.0
75	21005.0
76	21005.0
77	21005.0
78	14421.0
79	7837.0
80	7837.0
81	7837.0
82	5327.0
83	2817.0
84	2817.0
85	2817.0
86	1817.5
87	818.0
88	818.0
89	818.0
90	522.0
91	226.0
92	226.0
93	226.0
94	142.5
95	59.0
96	59.0
97	59.0
98	35.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030648438307104238
2	0.012942364865640462
3	0.017993043837597714
4	0.02011662476898883
5	0.018739166867545943
6	0.009125658597059128
7	0.0010330934260821653
8	0.0012626697429893132
9	9.756993468553784E-4
10	4.3045559420090224E-4
11	0.0012052756637625263
12	0.002927098040566135
13	0.0024679454067518397
14	6.026378318812631E-4
15	0.002267066129458085
16	0.0024679454067518397
17	0.0029557950801795286
18	0.002037489812550937
19	3.7306151497411527E-4
20	4.591526338142957E-4
21	0.08310662672038752
22	0.07822812998611063
23	0.07903164709528565
24	0.0010904875053089522
25	0.22105329614196997
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3484680.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.41272608257616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.60235153686347	66.26711391547603
2	10.340641325456334	16.009944688914366
3	2.2699686727785937	5.271733892455146
4	0.7347625712603377	2.2751989465882345
5	0.3419438743264342	1.3235403739423548
6	0.1813924677493561	0.8425251251594059
7	0.1142796843891613	0.6192691333094978
8	0.07520200702665715	0.4657273896651662
9	0.057452497648953875	0.40027990169324856
>10	0.2613575969645716	3.4700215927379423
>50	0.012801718987179406	0.6858066818904359
>100	0.006545887291198549	0.8919455820205355
>500	6.315059406803201E-4	0.34790002095198297
>1k	6.315059107241523E-4	0.9802337250727531
>5k	3.714740651318533E-5	0.1487590301229602
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5173	0.14844978592008448	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4900	0.14061549410562807	No Hit
CCCATGTACTCTGCGTTGATACCAC	4443	0.12750094700230724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.869703961339348E-5	0.0
8	2.869703961339348E-5	0.0	0.0	2.869703961339348E-5	0.0
9	2.869703961339348E-5	0.0	0.0	8.609111884018045E-5	0.0
10	2.869703961339348E-5	0.0	0.0	8.609111884018045E-5	0.0
11	2.869703961339348E-5	0.0	0.0	1.721822376803609E-4	0.0
12	2.869703961339348E-5	2.869703961339348E-5	0.0	1.721822376803609E-4	0.0
13	2.869703961339348E-5	2.869703961339348E-5	0.0	1.721822376803609E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	80	2.0063126E-6	13.059668	5
TATACCG	80	2.8751272E-5	11.872425	5
AGTACCG	65	8.042428E-4	11.689772	5
ACCGAGC	85	5.3520896E-5	11.171322	8
GTCTAGG	375	0.0	10.390082	1
GTTCTAG	415	0.0	10.304591	1
GCGTAAA	75	0.0026588119	10.12881	11
GTCTTAG	330	0.0	10.079071	1
TGGACCG	85	6.6104875E-4	10.056643	5
GTCCAGG	825	0.0	9.906287	1
CTAGGAC	395	0.0	9.860179	3
GGACTGT	465	0.0	9.598554	6
ACGGGTC	80	0.0045350296	9.495624	8
GACTGTC	350	0.0	9.495624	7
GTATTAG	455	0.0	9.189834	1
AGGACTG	715	0.0	9.165845	5
GGACCGT	105	4.5118757E-4	9.0442295	6
TCTGTCG	285	0.0	8.995854	8
TGTCGCC	320	0.0	8.902274	10
TGCGTTG	1560	0.0	8.887057	12
>>END_MODULE