FastQCFastQC Report
Wed 25 May 2016
SRR1294748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3484680
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT138300.3968800578532319No Hit
GGTATCAACGCAGAGTACTTTTTTT98270.2820058082808178No Hit
TATCAACGCAGAGTACTTTTTTTTT88140.2529357071524502No Hit
GTACATGGGAAGCAGTGGTATCAAC52260.14997072901959432No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49490.14202164904668435No Hit
CCCATGTACTCTGCGTTGATACCAC47830.137257940470861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC3900.011.2198543
TTAGGGT3250.09.9515234
TCAGGAC5000.09.7027353
AAGACCG900.00110093919.51248555
CGAAAAA900.00111975379.49487814
GTTCTAG3550.09.3825531
GTCTAGG4500.09.305111
TGTGCGC1451.7204093E-69.16746810
GTATTAG5300.09.1574731
GTGTAGG5650.09.0955011
GTCCTAA3250.09.0773621
AGCGCCC950.0018336188.99514710
TACTCCG850.00735891758.9529285
TTAAGAC3350.08.8025983
GTCTTAC3500.08.7008821
ACTAGAC2303.947207E-108.6853123
CCAGGAC8350.08.6580713
CTTAGAC2659.094947E-128.6150813
CTAGAAC4100.08.5844383
GTCTTGG5000.08.564931
Produced by FastQC (version 0.11.5)