FastQCFastQC Report
Wed 25 May 2016
SRR1294747_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294747_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4614787
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1671403.6218356340173443No Hit
CCCATGTACTCTGCGTTGATACCAC1590383.4462695678045376No Hit
GAGTACATGGGAAGCAGTGGTATCA751921.6293709763852589No Hit
CATGTACTCTGCGTTGATACCACTG589821.2781088271246321No Hit
GCGTTGATACCACTGCTTCCCATGT514341.114547648678043No Hit
GCTTCCCATGTACTCTGCGTTGATA449480.9739994500287878No Hit
ACGCAGAGTACATGGGAAGCAGTGG428620.9287969303892033No Hit
GTATCAACGCAGAGTACATGGGAAG413250.8954909511533252No Hit
ACTCTGCGTTGATACCACTGCTTCC344830.7472284202932876No Hit
TATCAACGCAGAGTACATGGGAAGC334540.7249305330885261No Hit
GGTATCAACGCAGAGTACATGGGAA279240.6050983501513721No Hit
CAGTGGTATCAACGCAGAGTACATG218750.47401971098557744No Hit
GTGGTATCAACGCAGAGTACATGGG212900.4613430695717917No Hit
GCAGTGGTATCAACGCAGAGTACAT182810.39613962681267845No Hit
ATACCACTGCTTCCCATGTACTCTG177040.3836363411789103No Hit
GTACTCTGCGTTGATACCACTGCTT168010.36406880750942566No Hit
CATGGGAAGCAGTGGTATCAACGCA160070.3468632463426806No Hit
CTGCTTCCCATGTACTCTGCGTTGA158890.34430624858742126No Hit
GTACATGGTAAGCAGTGGTATCAAC157730.3417925897771663No Hit
ACATGGGAAGCAGTGGTATCAACGC155600.33717699213419816No Hit
GGGAAGCAGTGGTATCAACGCAGAG154930.3357251374765509No Hit
TACCACTGCTTCCCATGTACTCTGC140650.3047811307434124No Hit
GATACCACTGCTTCCCATGTACTCT134450.29134605779204975No Hit
CTTCCCATGTACTCTGCGTTGATAC133630.2895691610468696No Hit
GCAGAGTACATGGGAAGCAGTGGTA128070.27752093433564756No Hit
GTTGATACCACTGCTTCCCATGTAC127880.27710921435810576No Hit
AAGCAGTGGTATCAACGCAGAGTAC125040.2709550841674816No Hit
CCACTGCTTCCCATGTACTCTGCGT123800.26826806957720906No Hit
CCATGTACTCTGCGTTGATACCACT117230.2540312261432651No Hit
ACCATGTACTCTGCGTTGATACCAC113960.24694530863504643No Hit
ATGGGAAGCAGTGGTATCAACGCAG103330.22391065936521012No Hit
TTCCCATGTACTCTGCGTTGATACC95960.20794025813109032No Hit
GTACATGGGTAAGCAGTGGTATCAA94550.20488486250828045No Hit
TGATACCACTGCTTCCCATGTACTC87990.1906696885468387No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA74890.1622826795689595No Hit
GGTAAGCAGTGGTATCAACGCAGAG72900.15797045454102215No Hit
CCCCATGTACTCTGCGTTGATACCA71760.1555001346757716No Hit
ATCAACGCAGAGTACATGGGAAGCA68630.14871758978258368No Hit
GCTTACCATGTACTCTGCGTTGATA59440.12880334455306386No Hit
GGAAGCAGTGGTATCAACGCAGAGT50960.11042763187120012No Hit
GAGTACATGGTAAGCAGTGGTATCA50480.10938749719109464No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA49800.10791397306094519No Hit
AAAAAGTACTCTGCGTTGATACCAC49090.10637544051328914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG658.0148526E-411.694711
GTACAAA11050.011.4367391
ACCGTCT855.3509146E-511.17174158
CGCAAAA3350.010.4923042
CTAGGAC2900.09.8268353
GTCTCGC1856.475602E-109.7587611
TAGGACC1856.511982E-109.7556934
GAACAAA20650.09.6630011
AGATCGA800.0045340989.4959798
CTTACAC4600.09.2927683
CTAGTAC1752.6775524E-89.2278653
GATACGA1252.739846E-59.124742518
TGTGCGC950.00183191668.9961918
TTAACGT850.00744603958.9402034
CCGTCTG850.0074659228.9372969
ATACGAC1401.0549524E-58.8418419
CTCTTAG3250.08.7710321
CTAAGAC2950.08.6942513
CTAGACT2950.08.6939694
GTCCTAG3300.08.6381381
Produced by FastQC (version 0.11.5)